Sulfitobacter phage NYA-2014a

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A088FAY9|A0A088FAY9_9CAUD Uncharacterized protein OS=Sulfitobacter phage NYA-2014a OX=1526550 GN=SUFP_058 PE=4 SV=1
MM1 pKa = 7.26TLYY4 pKa = 10.37TPIHH8 pKa = 6.39AAPLSRR14 pKa = 11.84PPRR17 pKa = 11.84LRR19 pKa = 11.84FIAAMPAEE27 pKa = 5.38DD28 pKa = 4.14SSQDD32 pKa = 3.32ASDD35 pKa = 3.13AWRR38 pKa = 11.84EE39 pKa = 4.03EE40 pKa = 4.08TGEE43 pKa = 4.01DD44 pKa = 4.37AIATAYY50 pKa = 9.57FRR52 pKa = 11.84HH53 pKa = 6.13EE54 pKa = 3.8PDD56 pKa = 4.57GYY58 pKa = 11.67GEE60 pKa = 4.48LCASLIGLSVQTDD73 pKa = 3.04ISLDD77 pKa = 3.43LFTPEE82 pKa = 3.86GATAFMGWEE91 pKa = 3.97TVNRR95 pKa = 11.84IEE97 pKa = 4.96NVQTQAIADD106 pKa = 3.99GVATT110 pKa = 4.39

Molecular weight: 11.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A088F6V5|A0A088F6V5_9CAUD DUF3307-containing protein OS=Sulfitobacter phage NYA-2014a OX=1526550 GN=SUFP_037 PE=4 SV=1
MM1 pKa = 8.21RR2 pKa = 11.84DD3 pKa = 3.58RR4 pKa = 11.84IVASIDD10 pKa = 3.15GLSWRR15 pKa = 11.84TCYY18 pKa = 10.56LVTGLRR24 pKa = 11.84ISWCGLAHH32 pKa = 6.01WAGRR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84VVWVLDD44 pKa = 4.52AILHH48 pKa = 5.44RR49 pKa = 11.84RR50 pKa = 11.84EE51 pKa = 3.88PDD53 pKa = 2.66HH54 pKa = 6.78CRR56 pKa = 11.84RR57 pKa = 11.84SFDD60 pKa = 2.47RR61 pKa = 11.84WYY63 pKa = 11.02

Molecular weight: 7.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

12984

42

935

182.9

19.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.192 ± 0.385

1.086 ± 0.113

6.816 ± 0.273

5.992 ± 0.377

3.05 ± 0.138

8.256 ± 0.49

2.041 ± 0.217

4.698 ± 0.171

4.629 ± 0.258

7.255 ± 0.249

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.765 ± 0.227

3.604 ± 0.203

4.382 ± 0.21

4.028 ± 0.317

6.593 ± 0.393

5.9 ± 0.293

5.977 ± 0.274

6.446 ± 0.306

1.61 ± 0.159

2.68 ± 0.144
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski