Anncaliia algerae PRA109

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Microsporidia; Tubulinosematoidea; Tubulinosematidae; Anncaliia; Anncaliia algerae

Average proteome isoelectric point is 7.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5143 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A059EI14|A0A059EI14_9MICR Uncharacterized protein (Fragment) OS=Anncaliia algerae PRA109 OX=1240240 GN=H311_04519 PE=4 SV=1
MM1 pKa = 7.78PEE3 pKa = 3.78MVIILSTDD11 pKa = 3.21NEE13 pKa = 4.41ILTSHH18 pKa = 7.36AANIKK23 pKa = 10.48DD24 pKa = 4.03EE25 pKa = 4.44EE26 pKa = 4.18EE27 pKa = 3.84MEE29 pKa = 4.23YY30 pKa = 10.21MNEE33 pKa = 4.57LIDD36 pKa = 3.68EE37 pKa = 4.37TLIMIKK43 pKa = 10.1NAPIPEE49 pKa = 5.0DD50 pKa = 3.61DD51 pKa = 3.39QKK53 pKa = 11.53FFYY56 pKa = 10.53GFNNTSPLVSVYY68 pKa = 10.72VLSDD72 pKa = 3.05GMKK75 pKa = 9.83ILLFTEE81 pKa = 4.94DD82 pKa = 3.58KK83 pKa = 11.32NSDD86 pKa = 3.34EE87 pKa = 5.15VYY89 pKa = 11.07DD90 pKa = 4.75LLLDD94 pKa = 5.48LYY96 pKa = 11.43DD97 pKa = 4.45CFKK100 pKa = 11.27NDD102 pKa = 3.3YY103 pKa = 11.13FMDD106 pKa = 4.37NDD108 pKa = 4.45DD109 pKa = 4.86FPDD112 pKa = 4.0NEE114 pKa = 4.36PSNDD118 pKa = 3.44QGPHH122 pKa = 5.24YY123 pKa = 10.73GVARR127 pKa = 4.06

Molecular weight: 14.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A059EIG8|A0A059EIG8_9MICR Uncharacterized protein (Fragment) OS=Anncaliia algerae PRA109 OX=1240240 GN=H311_04416 PE=4 SV=1
MM1 pKa = 7.93LIHH4 pKa = 7.29LISVLLCFLNEE15 pKa = 3.71ASAVCGTRR23 pKa = 11.84HH24 pKa = 4.62HH25 pKa = 6.8HH26 pKa = 5.9KK27 pKa = 9.95HH28 pKa = 6.03HH29 pKa = 6.26SHH31 pKa = 6.51HH32 pKa = 6.83SSHH35 pKa = 6.76HH36 pKa = 4.96SHH38 pKa = 7.25HH39 pKa = 6.81SRR41 pKa = 11.84DD42 pKa = 3.42PRR44 pKa = 11.84KK45 pKa = 9.28KK46 pKa = 9.72VLVIKK51 pKa = 10.05CVSSSSSSSSSSSSSSSSSSSSSSSSSSSSDD82 pKa = 3.3SNSICHH88 pKa = 6.7PCRR91 pKa = 11.84SSSSSSSSSSSSSSSSSSSSDD112 pKa = 3.0SDD114 pKa = 3.62SSSICHH120 pKa = 6.27RR121 pKa = 11.84RR122 pKa = 11.84RR123 pKa = 11.84SSRR126 pKa = 11.84RR127 pKa = 11.84CRR129 pKa = 11.84PSRR132 pKa = 11.84RR133 pKa = 11.84SHH135 pKa = 5.18FSEE138 pKa = 3.72RR139 pKa = 11.84KK140 pKa = 7.68YY141 pKa = 11.01VRR143 pKa = 11.84TVIHH147 pKa = 6.35FLKK150 pKa = 10.26KK151 pKa = 9.76VYY153 pKa = 8.56WVKK156 pKa = 9.56GTKK159 pKa = 9.75HH160 pKa = 6.25IPDD163 pKa = 3.41EE164 pKa = 4.3RR165 pKa = 11.84RR166 pKa = 11.84VKK168 pKa = 10.67GMLRR172 pKa = 11.84YY173 pKa = 9.38FYY175 pKa = 10.81RR176 pKa = 11.84EE177 pKa = 4.05FGRR180 pKa = 11.84NPHH183 pKa = 6.43LLIIFTANTIFCTSGYY199 pKa = 8.17TRR201 pKa = 4.27

Molecular weight: 22.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5143

0

5143

993622

22

843

193.2

22.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.953 ± 0.03

1.867 ± 0.019

5.037 ± 0.036

7.565 ± 0.051

6.356 ± 0.05

3.462 ± 0.035

1.82 ± 0.018

9.891 ± 0.041

10.502 ± 0.055

10.014 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.092 ± 0.016

8.069 ± 0.045

2.425 ± 0.024

2.588 ± 0.023

3.58 ± 0.027

7.211 ± 0.037

4.546 ± 0.025

4.708 ± 0.032

0.564 ± 0.009

4.748 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski