Tomitella sp. HY188

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Corynebacteriales incertae sedis; Tomitella; unclassified Tomitella

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3520 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A516X4E8|A0A516X4E8_9ACTN Uncharacterized protein OS=Tomitella sp. HY188 OX=2597660 GN=FO059_12290 PE=4 SV=1
MM1 pKa = 7.85TDD3 pKa = 3.34TGPQDD8 pKa = 4.26RR9 pKa = 11.84SDD11 pKa = 4.37DD12 pKa = 3.86DD13 pKa = 4.03TPGVSGNRR21 pKa = 11.84GSGGGYY27 pKa = 10.27GDD29 pKa = 5.37ADD31 pKa = 3.93PASGDD36 pKa = 3.75TADD39 pKa = 4.81LDD41 pKa = 3.97SLGRR45 pKa = 11.84RR46 pKa = 11.84GSPYY50 pKa = 10.6DD51 pKa = 3.39DD52 pKa = 3.32TAFGQEE58 pKa = 4.49SVDD61 pKa = 3.4SGGPEE66 pKa = 3.98SGGSASGYY74 pKa = 10.42DD75 pKa = 3.9ADD77 pKa = 4.39EE78 pKa = 4.06AWTIRR83 pKa = 11.84KK84 pKa = 7.91EE85 pKa = 4.36SPGPGWTTQAGAGSGPGWGSAPLYY109 pKa = 10.42EE110 pKa = 5.66SDD112 pKa = 3.4ATPFGTSYY120 pKa = 11.69GEE122 pKa = 3.99TARR125 pKa = 11.84YY126 pKa = 7.99EE127 pKa = 4.13ADD129 pKa = 2.99AVEE132 pKa = 4.62AAEE135 pKa = 4.36SAPVDD140 pKa = 3.77PEE142 pKa = 5.41ADD144 pKa = 3.61DD145 pKa = 4.81VEE147 pKa = 5.49PDD149 pKa = 3.55YY150 pKa = 11.55SDD152 pKa = 5.9PIADD156 pKa = 3.96DD157 pKa = 4.14APRR160 pKa = 11.84HH161 pKa = 5.85DD162 pKa = 4.75ADD164 pKa = 4.95AGAEE168 pKa = 4.41GPGPGTPPPGMPPPPPGMPPPPPGGEE194 pKa = 4.04YY195 pKa = 10.98GPAGYY200 pKa = 9.19GAPGYY205 pKa = 10.12APPGYY210 pKa = 10.41GPAGYY215 pKa = 9.04GAPGYY220 pKa = 10.41GPAGYY225 pKa = 10.49GPGPGMWAPDD235 pKa = 3.21PTAPYY240 pKa = 9.78GRR242 pKa = 11.84HH243 pKa = 5.64PVTGEE248 pKa = 3.8AFSDD252 pKa = 3.73KK253 pKa = 10.59QKK255 pKa = 11.06LVAGLLQIFVGWTGAGRR272 pKa = 11.84FYY274 pKa = 11.07LGDD277 pKa = 3.36NGMGVAQLLVSVFTCGIGALWPLIDD302 pKa = 5.92GILILMGNVRR312 pKa = 11.84DD313 pKa = 4.0SQGRR317 pKa = 11.84PLRR320 pKa = 11.84DD321 pKa = 3.0

Molecular weight: 32.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A516X8C4|A0A516X8C4_9ACTN DUF3043 domain-containing protein OS=Tomitella sp. HY188 OX=2597660 GN=FO059_12025 PE=4 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.53GKK5 pKa = 8.66RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84SIVANRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.63GRR42 pKa = 11.84AALSAA47 pKa = 3.96

Molecular weight: 5.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3520

0

3520

1168249

24

4704

331.9

35.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.916 ± 0.064

0.77 ± 0.012

6.599 ± 0.033

5.395 ± 0.037

2.819 ± 0.023

9.932 ± 0.037

2.179 ± 0.02

3.857 ± 0.028

1.849 ± 0.029

9.303 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.087 ± 0.016

1.753 ± 0.02

5.815 ± 0.027

2.695 ± 0.023

7.84 ± 0.046

5.278 ± 0.028

5.817 ± 0.029

8.742 ± 0.038

1.39 ± 0.015

1.963 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski