Amino acid dipepetide frequency for Roseobacter phage CRP-5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.57AlaAla: 7.57 ± 1.337
0.637AlaCys: 0.637 ± 0.233
5.657AlaAsp: 5.657 ± 0.602
6.932AlaGlu: 6.932 ± 0.627
2.629AlaPhe: 2.629 ± 0.33
5.418AlaGly: 5.418 ± 0.763
1.434AlaHis: 1.434 ± 0.458
4.303AlaIle: 4.303 ± 0.7
5.976AlaLys: 5.976 ± 0.864
6.215AlaLeu: 6.215 ± 0.751
1.594AlaMet: 1.594 ± 0.41
4.382AlaAsn: 4.382 ± 0.63
2.948AlaPro: 2.948 ± 0.458
3.187AlaGln: 3.187 ± 0.645
3.506AlaArg: 3.506 ± 0.612
5.657AlaSer: 5.657 ± 0.963
6.693AlaThr: 6.693 ± 0.723
5.179AlaVal: 5.179 ± 1.081
1.514AlaTrp: 1.514 ± 0.3
3.028AlaTyr: 3.028 ± 0.415
0.0AlaXaa: 0.0 ± 0.0
Cys
0.478CysAla: 0.478 ± 0.253
0.0CysCys: 0.0 ± 0.0
0.558CysAsp: 0.558 ± 0.214
0.797CysGlu: 0.797 ± 0.336
0.478CysPhe: 0.478 ± 0.21
0.478CysGly: 0.478 ± 0.275
0.637CysHis: 0.637 ± 0.299
0.319CysIle: 0.319 ± 0.18
1.116CysLys: 1.116 ± 0.419
0.478CysLeu: 0.478 ± 0.208
0.08CysMet: 0.08 ± 0.067
0.398CysAsn: 0.398 ± 0.178
0.159CysPro: 0.159 ± 0.121
0.239CysGln: 0.239 ± 0.144
0.239CysArg: 0.239 ± 0.145
0.558CysSer: 0.558 ± 0.333
0.239CysThr: 0.239 ± 0.203
0.637CysVal: 0.637 ± 0.287
0.08CysTrp: 0.08 ± 0.094
0.637CysTyr: 0.637 ± 0.265
0.0CysXaa: 0.0 ± 0.0
Asp
4.622AspAla: 4.622 ± 0.48
0.478AspCys: 0.478 ± 0.226
4.143AspAsp: 4.143 ± 0.646
4.701AspGlu: 4.701 ± 0.645
3.108AspPhe: 3.108 ± 0.608
5.259AspGly: 5.259 ± 0.862
1.355AspHis: 1.355 ± 0.365
4.701AspIle: 4.701 ± 0.567
4.143AspLys: 4.143 ± 0.684
5.179AspLeu: 5.179 ± 0.642
1.673AspMet: 1.673 ± 0.406
3.745AspAsn: 3.745 ± 0.677
3.108AspPro: 3.108 ± 0.514
2.311AspGln: 2.311 ± 0.581
2.709AspArg: 2.709 ± 0.538
3.984AspSer: 3.984 ± 0.618
3.665AspThr: 3.665 ± 0.636
3.825AspVal: 3.825 ± 0.593
1.116AspTrp: 1.116 ± 0.342
2.151AspTyr: 2.151 ± 0.409
0.0AspXaa: 0.0 ± 0.0
Glu
6.614GluAla: 6.614 ± 0.709
0.478GluCys: 0.478 ± 0.208
5.418GluAsp: 5.418 ± 0.68
5.339GluGlu: 5.339 ± 0.872
2.55GluPhe: 2.55 ± 0.324
3.665GluGly: 3.665 ± 0.529
1.434GluHis: 1.434 ± 0.343
3.108GluIle: 3.108 ± 0.573
3.825GluLys: 3.825 ± 0.423
6.056GluLeu: 6.056 ± 0.761
2.231GluMet: 2.231 ± 0.463
2.948GluAsn: 2.948 ± 0.521
1.992GluPro: 1.992 ± 0.437
3.506GluGln: 3.506 ± 0.588
3.108GluArg: 3.108 ± 0.462
3.267GluSer: 3.267 ± 0.475
4.382GluThr: 4.382 ± 0.479
3.904GluVal: 3.904 ± 0.473
1.514GluTrp: 1.514 ± 0.463
2.789GluTyr: 2.789 ± 0.448
0.0GluXaa: 0.0 ± 0.0
Phe
3.187PheAla: 3.187 ± 0.751
0.398PheCys: 0.398 ± 0.22
2.311PheAsp: 2.311 ± 0.364
2.629PheGlu: 2.629 ± 0.534
1.116PhePhe: 1.116 ± 0.288
3.347PheGly: 3.347 ± 0.572
0.876PheHis: 0.876 ± 0.374
1.992PheIle: 1.992 ± 0.345
2.948PheLys: 2.948 ± 0.466
2.869PheLeu: 2.869 ± 0.439
1.036PheMet: 1.036 ± 0.279
2.311PheAsn: 2.311 ± 0.357
1.195PhePro: 1.195 ± 0.171
1.116PheGln: 1.116 ± 0.263
2.151PheArg: 2.151 ± 0.422
3.028PheSer: 3.028 ± 0.535
2.39PheThr: 2.39 ± 0.43
2.709PheVal: 2.709 ± 0.366
0.717PheTrp: 0.717 ± 0.325
1.594PheTyr: 1.594 ± 0.335
0.0PheXaa: 0.0 ± 0.0
Gly
4.701GlyAla: 4.701 ± 0.862
0.319GlyCys: 0.319 ± 0.19
4.223GlyAsp: 4.223 ± 0.619
4.143GlyGlu: 4.143 ± 0.827
3.665GlyPhe: 3.665 ± 0.516
4.223GlyGly: 4.223 ± 0.616
0.956GlyHis: 0.956 ± 0.355
4.143GlyIle: 4.143 ± 0.784
4.542GlyLys: 4.542 ± 0.59
4.861GlyLeu: 4.861 ± 0.863
1.434GlyMet: 1.434 ± 0.384
4.223GlyAsn: 4.223 ± 0.744
1.514GlyPro: 1.514 ± 0.382
2.311GlyGln: 2.311 ± 0.441
3.347GlyArg: 3.347 ± 0.526
4.861GlySer: 4.861 ± 0.652
5.578GlyThr: 5.578 ± 1.251
5.578GlyVal: 5.578 ± 0.719
1.195GlyTrp: 1.195 ± 0.291
2.231GlyTyr: 2.231 ± 0.396
0.0GlyXaa: 0.0 ± 0.0
His
1.036HisAla: 1.036 ± 0.396
0.398HisCys: 0.398 ± 0.17
0.637HisAsp: 0.637 ± 0.224
1.116HisGlu: 1.116 ± 0.308
0.956HisPhe: 0.956 ± 0.288
0.876HisGly: 0.876 ± 0.336
0.239HisHis: 0.239 ± 0.165
1.275HisIle: 1.275 ± 0.357
1.116HisLys: 1.116 ± 0.347
1.355HisLeu: 1.355 ± 0.37
0.398HisMet: 0.398 ± 0.165
0.478HisAsn: 0.478 ± 0.205
0.637HisPro: 0.637 ± 0.267
0.319HisGln: 0.319 ± 0.17
1.116HisArg: 1.116 ± 0.3
1.195HisSer: 1.195 ± 0.299
1.036HisThr: 1.036 ± 0.292
1.116HisVal: 1.116 ± 0.381
0.319HisTrp: 0.319 ± 0.172
0.637HisTyr: 0.637 ± 0.252
0.0HisXaa: 0.0 ± 0.0
Ile
4.622IleAla: 4.622 ± 0.552
0.637IleCys: 0.637 ± 0.232
3.665IleAsp: 3.665 ± 0.65
4.781IleGlu: 4.781 ± 0.823
2.39IlePhe: 2.39 ± 0.378
3.028IleGly: 3.028 ± 0.437
0.797IleHis: 0.797 ± 0.257
2.629IleIle: 2.629 ± 0.46
3.267IleLys: 3.267 ± 0.564
3.347IleLeu: 3.347 ± 0.522
1.275IleMet: 1.275 ± 0.321
2.789IleAsn: 2.789 ± 0.573
1.673IlePro: 1.673 ± 0.351
2.47IleGln: 2.47 ± 0.41
3.426IleArg: 3.426 ± 0.513
4.462IleSer: 4.462 ± 0.537
3.825IleThr: 3.825 ± 0.856
4.143IleVal: 4.143 ± 0.635
0.637IleTrp: 0.637 ± 0.24
1.594IleTyr: 1.594 ± 0.39
0.0IleXaa: 0.0 ± 0.0
Lys
5.737LysAla: 5.737 ± 0.672
0.717LysCys: 0.717 ± 0.388
5.737LysAsp: 5.737 ± 0.69
3.825LysGlu: 3.825 ± 0.639
1.912LysPhe: 1.912 ± 0.361
4.542LysGly: 4.542 ± 0.778
1.594LysHis: 1.594 ± 0.485
3.187LysIle: 3.187 ± 0.471
4.94LysLys: 4.94 ± 1.111
5.578LysLeu: 5.578 ± 0.745
1.355LysMet: 1.355 ± 0.28
2.55LysAsn: 2.55 ± 0.457
2.311LysPro: 2.311 ± 0.395
3.028LysGln: 3.028 ± 0.676
2.47LysArg: 2.47 ± 0.544
3.347LysSer: 3.347 ± 0.558
5.179LysThr: 5.179 ± 0.543
4.781LysVal: 4.781 ± 0.55
0.876LysTrp: 0.876 ± 0.281
2.151LysTyr: 2.151 ± 0.598
0.0LysXaa: 0.0 ± 0.0
Leu
5.657LeuAla: 5.657 ± 0.615
0.558LeuCys: 0.558 ± 0.232
5.976LeuAsp: 5.976 ± 0.791
5.179LeuGlu: 5.179 ± 0.532
3.187LeuPhe: 3.187 ± 0.436
6.135LeuGly: 6.135 ± 0.956
0.398LeuHis: 0.398 ± 0.154
3.347LeuIle: 3.347 ± 0.49
4.303LeuLys: 4.303 ± 0.496
6.454LeuLeu: 6.454 ± 0.681
2.151LeuMet: 2.151 ± 0.411
4.382LeuAsn: 4.382 ± 0.4
1.992LeuPro: 1.992 ± 0.443
2.55LeuGln: 2.55 ± 0.463
3.506LeuArg: 3.506 ± 0.495
6.693LeuSer: 6.693 ± 0.788
7.092LeuThr: 7.092 ± 0.69
5.02LeuVal: 5.02 ± 0.623
0.876LeuTrp: 0.876 ± 0.25
2.39LeuTyr: 2.39 ± 0.393
0.0LeuXaa: 0.0 ± 0.0
Met
2.948MetAla: 2.948 ± 0.501
0.558MetCys: 0.558 ± 0.215
1.833MetAsp: 1.833 ± 0.405
1.514MetGlu: 1.514 ± 0.344
1.036MetPhe: 1.036 ± 0.233
1.355MetGly: 1.355 ± 0.431
0.239MetHis: 0.239 ± 0.16
1.594MetIle: 1.594 ± 0.397
1.434MetLys: 1.434 ± 0.336
2.151MetLeu: 2.151 ± 0.423
0.478MetMet: 0.478 ± 0.229
0.717MetAsn: 0.717 ± 0.319
1.036MetPro: 1.036 ± 0.267
0.876MetGln: 0.876 ± 0.254
1.673MetArg: 1.673 ± 0.321
1.673MetSer: 1.673 ± 0.378
1.992MetThr: 1.992 ± 0.418
1.434MetVal: 1.434 ± 0.319
0.398MetTrp: 0.398 ± 0.177
0.558MetTyr: 0.558 ± 0.218
0.0MetXaa: 0.0 ± 0.0
Asn
4.382AsnAla: 4.382 ± 0.452
0.637AsnCys: 0.637 ± 0.218
2.948AsnAsp: 2.948 ± 0.439
1.753AsnGlu: 1.753 ± 0.302
1.673AsnPhe: 1.673 ± 0.415
3.426AsnGly: 3.426 ± 0.714
0.717AsnHis: 0.717 ± 0.327
3.426AsnIle: 3.426 ± 0.53
3.665AsnLys: 3.665 ± 0.633
4.223AsnLeu: 4.223 ± 0.463
1.753AsnMet: 1.753 ± 0.399
1.912AsnAsn: 1.912 ± 0.454
2.629AsnPro: 2.629 ± 0.387
1.594AsnGln: 1.594 ± 0.302
2.869AsnArg: 2.869 ± 0.499
2.231AsnSer: 2.231 ± 0.473
3.586AsnThr: 3.586 ± 0.635
3.028AsnVal: 3.028 ± 0.513
0.797AsnTrp: 0.797 ± 0.233
1.594AsnTyr: 1.594 ± 0.355
0.0AsnXaa: 0.0 ± 0.0
Pro
3.028ProAla: 3.028 ± 0.491
0.319ProCys: 0.319 ± 0.173
2.39ProAsp: 2.39 ± 0.345
3.108ProGlu: 3.108 ± 0.43
1.195ProPhe: 1.195 ± 0.305
0.478ProGly: 0.478 ± 0.174
0.637ProHis: 0.637 ± 0.227
1.116ProIle: 1.116 ± 0.272
2.789ProLys: 2.789 ± 0.435
1.833ProLeu: 1.833 ± 0.387
1.116ProMet: 1.116 ± 0.356
1.195ProAsn: 1.195 ± 0.351
0.717ProPro: 0.717 ± 0.233
1.116ProGln: 1.116 ± 0.299
1.275ProArg: 1.275 ± 0.365
3.506ProSer: 3.506 ± 0.417
2.869ProThr: 2.869 ± 0.387
2.311ProVal: 2.311 ± 0.4
0.637ProTrp: 0.637 ± 0.245
1.434ProTyr: 1.434 ± 0.253
0.0ProXaa: 0.0 ± 0.0
Gln
4.861GlnAla: 4.861 ± 0.734
0.159GlnCys: 0.159 ± 0.128
1.673GlnAsp: 1.673 ± 0.339
2.789GlnGlu: 2.789 ± 0.4
1.673GlnPhe: 1.673 ± 0.328
2.709GlnGly: 2.709 ± 0.443
0.558GlnHis: 0.558 ± 0.194
2.072GlnIle: 2.072 ± 0.534
2.47GlnLys: 2.47 ± 0.518
4.303GlnLeu: 4.303 ± 0.651
1.116GlnMet: 1.116 ± 0.325
1.833GlnAsn: 1.833 ± 0.393
0.956GlnPro: 0.956 ± 0.282
1.833GlnGln: 1.833 ± 0.488
1.594GlnArg: 1.594 ± 0.407
2.072GlnSer: 2.072 ± 0.443
2.231GlnThr: 2.231 ± 0.428
2.311GlnVal: 2.311 ± 0.42
0.398GlnTrp: 0.398 ± 0.182
1.355GlnTyr: 1.355 ± 0.459
0.0GlnXaa: 0.0 ± 0.0
Arg
3.187ArgAla: 3.187 ± 0.489
0.319ArgCys: 0.319 ± 0.175
2.709ArgAsp: 2.709 ± 0.373
2.47ArgGlu: 2.47 ± 0.504
2.311ArgPhe: 2.311 ± 0.48
2.629ArgGly: 2.629 ± 0.517
0.956ArgHis: 0.956 ± 0.329
3.267ArgIle: 3.267 ± 0.718
3.745ArgLys: 3.745 ± 0.644
4.462ArgLeu: 4.462 ± 0.436
1.833ArgMet: 1.833 ± 0.327
1.912ArgAsn: 1.912 ± 0.387
1.912ArgPro: 1.912 ± 0.361
1.912ArgGln: 1.912 ± 0.404
2.231ArgArg: 2.231 ± 0.366
3.028ArgSer: 3.028 ± 0.354
2.629ArgThr: 2.629 ± 0.408
3.347ArgVal: 3.347 ± 0.564
0.797ArgTrp: 0.797 ± 0.204
2.39ArgTyr: 2.39 ± 0.519
0.0ArgXaa: 0.0 ± 0.0
Ser
6.614SerAla: 6.614 ± 0.784
0.478SerCys: 0.478 ± 0.225
4.94SerAsp: 4.94 ± 0.723
4.064SerGlu: 4.064 ± 0.67
3.665SerPhe: 3.665 ± 0.517
7.012SerGly: 7.012 ± 1.181
0.717SerHis: 0.717 ± 0.265
3.745SerIle: 3.745 ± 0.663
4.143SerLys: 4.143 ± 0.63
5.896SerLeu: 5.896 ± 0.695
1.275SerMet: 1.275 ± 0.336
3.347SerAsn: 3.347 ± 0.492
2.151SerPro: 2.151 ± 0.336
2.151SerGln: 2.151 ± 0.457
3.108SerArg: 3.108 ± 0.553
5.179SerSer: 5.179 ± 1.049
4.064SerThr: 4.064 ± 0.742
3.347SerVal: 3.347 ± 0.631
0.637SerTrp: 0.637 ± 0.204
1.992SerTyr: 1.992 ± 0.395
0.0SerXaa: 0.0 ± 0.0
Thr
5.737ThrAla: 5.737 ± 0.822
0.319ThrCys: 0.319 ± 0.165
3.665ThrAsp: 3.665 ± 0.568
5.179ThrGlu: 5.179 ± 0.75
2.231ThrPhe: 2.231 ± 0.407
5.179ThrGly: 5.179 ± 0.768
1.116ThrHis: 1.116 ± 0.296
5.498ThrIle: 5.498 ± 0.668
4.542ThrLys: 4.542 ± 0.704
5.02ThrLeu: 5.02 ± 0.732
1.912ThrMet: 1.912 ± 0.415
3.825ThrAsn: 3.825 ± 0.574
2.151ThrPro: 2.151 ± 0.396
3.506ThrGln: 3.506 ± 0.539
4.303ThrArg: 4.303 ± 0.645
4.94ThrSer: 4.94 ± 0.853
5.179ThrThr: 5.179 ± 0.602
4.382ThrVal: 4.382 ± 0.524
1.116ThrTrp: 1.116 ± 0.337
2.151ThrTyr: 2.151 ± 0.417
0.0ThrXaa: 0.0 ± 0.0
Val
5.1ValAla: 5.1 ± 0.697
0.478ValCys: 0.478 ± 0.193
4.143ValAsp: 4.143 ± 0.545
4.462ValGlu: 4.462 ± 0.516
2.072ValPhe: 2.072 ± 0.382
4.781ValGly: 4.781 ± 0.607
0.797ValHis: 0.797 ± 0.356
3.426ValIle: 3.426 ± 0.494
4.861ValLys: 4.861 ± 0.642
4.143ValLeu: 4.143 ± 0.563
1.434ValMet: 1.434 ± 0.35
3.426ValAsn: 3.426 ± 0.419
2.231ValPro: 2.231 ± 0.369
2.47ValGln: 2.47 ± 0.458
3.108ValArg: 3.108 ± 0.436
5.976ValSer: 5.976 ± 0.69
4.701ValThr: 4.701 ± 0.521
3.665ValVal: 3.665 ± 0.622
0.797ValTrp: 0.797 ± 0.278
2.231ValTyr: 2.231 ± 0.467
0.0ValXaa: 0.0 ± 0.0
Trp
1.116TrpAla: 1.116 ± 0.257
0.159TrpCys: 0.159 ± 0.1
1.434TrpAsp: 1.434 ± 0.428
0.797TrpGlu: 0.797 ± 0.27
0.637TrpPhe: 0.637 ± 0.208
1.116TrpGly: 1.116 ± 0.333
0.398TrpHis: 0.398 ± 0.169
0.159TrpIle: 0.159 ± 0.094
0.876TrpLys: 0.876 ± 0.243
1.355TrpLeu: 1.355 ± 0.324
0.478TrpMet: 0.478 ± 0.164
0.637TrpAsn: 0.637 ± 0.217
0.478TrpPro: 0.478 ± 0.226
0.797TrpGln: 0.797 ± 0.235
0.717TrpArg: 0.717 ± 0.286
0.956TrpSer: 0.956 ± 0.234
1.195TrpThr: 1.195 ± 0.44
1.036TrpVal: 1.036 ± 0.24
0.319TrpTrp: 0.319 ± 0.128
0.637TrpTyr: 0.637 ± 0.214
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.347TyrAla: 3.347 ± 0.429
0.558TyrCys: 0.558 ± 0.245
1.912TyrAsp: 1.912 ± 0.386
2.55TyrGlu: 2.55 ± 0.479
1.434TyrPhe: 1.434 ± 0.317
2.39TyrGly: 2.39 ± 0.473
0.398TyrHis: 0.398 ± 0.185
2.151TyrIle: 2.151 ± 0.328
1.116TyrLys: 1.116 ± 0.262
2.072TyrLeu: 2.072 ± 0.357
0.797TyrMet: 0.797 ± 0.261
1.833TyrAsn: 1.833 ± 0.515
1.275TyrPro: 1.275 ± 0.445
1.673TyrGln: 1.673 ± 0.333
1.594TyrArg: 1.594 ± 0.335
1.912TyrSer: 1.912 ± 0.427
3.426TyrThr: 3.426 ± 0.576
2.55TyrVal: 2.55 ± 0.465
0.558TyrTrp: 0.558 ± 0.224
1.434TyrTyr: 1.434 ± 0.445
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (12551 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski