Amino acid dipepetide frequency for Rhodococcus phage ReqiDocB7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.1AlaAla: 11.1 ± 3.019
0.68AlaCys: 0.68 ± 0.193
5.316AlaAsp: 5.316 ± 0.418
6.465AlaGlu: 6.465 ± 0.506
2.637AlaPhe: 2.637 ± 0.35
7.06AlaGly: 7.06 ± 0.879
1.403AlaHis: 1.403 ± 0.226
4.721AlaIle: 4.721 ± 0.868
5.231AlaLys: 5.231 ± 0.61
7.06AlaLeu: 7.06 ± 0.945
2.764AlaMet: 2.764 ± 0.466
3.147AlaAsn: 3.147 ± 0.337
3.615AlaPro: 3.615 ± 0.446
4.04AlaGln: 4.04 ± 0.599
5.656AlaArg: 5.656 ± 0.46
5.444AlaSer: 5.444 ± 0.831
6.592AlaThr: 6.592 ± 1.1
5.954AlaVal: 5.954 ± 0.54
1.659AlaTrp: 1.659 ± 0.233
2.424AlaTyr: 2.424 ± 0.315
0.0AlaXaa: 0.0 ± 0.0
Cys
0.68CysAla: 0.68 ± 0.19
0.213CysCys: 0.213 ± 0.1
0.68CysAsp: 0.68 ± 0.197
0.468CysGlu: 0.468 ± 0.144
0.085CysPhe: 0.085 ± 0.068
0.68CysGly: 0.68 ± 0.204
0.468CysHis: 0.468 ± 0.159
0.17CysIle: 0.17 ± 0.095
0.595CysLys: 0.595 ± 0.182
0.638CysLeu: 0.638 ± 0.163
0.34CysMet: 0.34 ± 0.127
0.34CysAsn: 0.34 ± 0.127
0.595CysPro: 0.595 ± 0.186
0.213CysGln: 0.213 ± 0.1
0.68CysArg: 0.68 ± 0.216
0.595CysSer: 0.595 ± 0.181
0.978CysThr: 0.978 ± 0.238
0.51CysVal: 0.51 ± 0.146
0.17CysTrp: 0.17 ± 0.118
0.255CysTyr: 0.255 ± 0.106
0.0CysXaa: 0.0 ± 0.0
Asp
5.997AspAla: 5.997 ± 0.623
0.723AspCys: 0.723 ± 0.168
3.232AspAsp: 3.232 ± 0.424
4.04AspGlu: 4.04 ± 0.478
2.084AspPhe: 2.084 ± 0.287
5.359AspGly: 5.359 ± 0.489
1.489AspHis: 1.489 ± 0.288
3.53AspIle: 3.53 ± 0.352
3.147AspLys: 3.147 ± 0.395
4.508AspLeu: 4.508 ± 0.429
1.871AspMet: 1.871 ± 0.295
2.297AspAsn: 2.297 ± 0.25
3.913AspPro: 3.913 ± 0.535
2.041AspGln: 2.041 ± 0.26
3.615AspArg: 3.615 ± 0.444
3.7AspSer: 3.7 ± 0.467
2.807AspThr: 2.807 ± 0.33
3.487AspVal: 3.487 ± 0.364
1.403AspTrp: 1.403 ± 0.266
1.999AspTyr: 1.999 ± 0.314
0.0AspXaa: 0.0 ± 0.0
Glu
5.742GluAla: 5.742 ± 0.664
0.553GluCys: 0.553 ± 0.174
3.7GluAsp: 3.7 ± 0.426
5.274GluGlu: 5.274 ± 0.725
2.849GluPhe: 2.849 ± 0.366
4.891GluGly: 4.891 ± 0.514
1.148GluHis: 1.148 ± 0.238
3.445GluIle: 3.445 ± 0.379
3.913GluLys: 3.913 ± 0.368
5.869GluLeu: 5.869 ± 0.448
2.084GluMet: 2.084 ± 0.28
3.062GluAsn: 3.062 ± 0.31
2.594GluPro: 2.594 ± 0.266
3.232GluGln: 3.232 ± 0.414
3.402GluArg: 3.402 ± 0.496
3.02GluSer: 3.02 ± 0.343
4.168GluThr: 4.168 ± 0.479
4.508GluVal: 4.508 ± 0.589
1.489GluTrp: 1.489 ± 0.32
2.339GluTyr: 2.339 ± 0.388
0.0GluXaa: 0.0 ± 0.0
Phe
2.637PheAla: 2.637 ± 0.323
0.298PheCys: 0.298 ± 0.118
2.382PheAsp: 2.382 ± 0.338
2.424PheGlu: 2.424 ± 0.354
1.276PhePhe: 1.276 ± 0.323
3.062PheGly: 3.062 ± 0.507
0.851PheHis: 0.851 ± 0.18
1.914PheIle: 1.914 ± 0.288
2.041PheLys: 2.041 ± 0.344
2.424PheLeu: 2.424 ± 0.376
1.276PheMet: 1.276 ± 0.24
1.531PheAsn: 1.531 ± 0.27
1.616PhePro: 1.616 ± 0.312
1.786PheGln: 1.786 ± 0.271
1.574PheArg: 1.574 ± 0.264
2.594PheSer: 2.594 ± 0.385
2.126PheThr: 2.126 ± 0.276
1.956PheVal: 1.956 ± 0.299
0.383PheTrp: 0.383 ± 0.125
0.766PheTyr: 0.766 ± 0.207
0.0PheXaa: 0.0 ± 0.0
Gly
6.039GlyAla: 6.039 ± 1.074
0.893GlyCys: 0.893 ± 0.294
4.636GlyAsp: 4.636 ± 0.319
4.21GlyGlu: 4.21 ± 0.524
3.02GlyPhe: 3.02 ± 0.35
6.082GlyGly: 6.082 ± 0.749
1.616GlyHis: 1.616 ± 0.379
4.806GlyIle: 4.806 ± 0.417
4.763GlyLys: 4.763 ± 0.504
5.997GlyLeu: 5.997 ± 0.535
2.212GlyMet: 2.212 ± 0.38
3.487GlyAsn: 3.487 ± 0.55
3.19GlyPro: 3.19 ± 0.374
2.339GlyGln: 2.339 ± 0.307
3.572GlyArg: 3.572 ± 0.352
5.104GlySer: 5.104 ± 0.499
6.209GlyThr: 6.209 ± 0.637
5.019GlyVal: 5.019 ± 0.558
1.956GlyTrp: 1.956 ± 0.238
2.297GlyTyr: 2.297 ± 0.27
0.0GlyXaa: 0.0 ± 0.0
His
1.701HisAla: 1.701 ± 0.262
0.213HisCys: 0.213 ± 0.126
1.021HisAsp: 1.021 ± 0.252
1.361HisGlu: 1.361 ± 0.305
0.68HisPhe: 0.68 ± 0.195
1.446HisGly: 1.446 ± 0.337
0.383HisHis: 0.383 ± 0.106
1.531HisIle: 1.531 ± 0.274
0.766HisLys: 0.766 ± 0.257
1.786HisLeu: 1.786 ± 0.355
0.425HisMet: 0.425 ± 0.132
0.978HisAsn: 0.978 ± 0.181
0.978HisPro: 0.978 ± 0.194
0.978HisGln: 0.978 ± 0.23
1.021HisArg: 1.021 ± 0.242
1.106HisSer: 1.106 ± 0.194
1.191HisThr: 1.191 ± 0.216
1.361HisVal: 1.361 ± 0.256
0.34HisTrp: 0.34 ± 0.128
0.808HisTyr: 0.808 ± 0.2
0.0HisXaa: 0.0 ± 0.0
Ile
5.699IleAla: 5.699 ± 0.514
0.595IleCys: 0.595 ± 0.173
3.19IleAsp: 3.19 ± 0.383
4.423IleGlu: 4.423 ± 0.4
1.574IlePhe: 1.574 ± 0.264
3.572IleGly: 3.572 ± 0.358
1.318IleHis: 1.318 ± 0.197
2.169IleIle: 2.169 ± 0.289
3.36IleLys: 3.36 ± 0.347
4.253IleLeu: 4.253 ± 0.442
1.403IleMet: 1.403 ± 0.244
2.594IleAsn: 2.594 ± 0.326
2.041IlePro: 2.041 ± 0.322
1.489IleGln: 1.489 ± 0.225
2.849IleArg: 2.849 ± 0.404
3.36IleSer: 3.36 ± 0.527
3.402IleThr: 3.402 ± 0.472
3.7IleVal: 3.7 ± 0.447
1.063IleTrp: 1.063 ± 0.227
1.489IleTyr: 1.489 ± 0.267
0.0IleXaa: 0.0 ± 0.0
Lys
5.401LysAla: 5.401 ± 0.59
0.298LysCys: 0.298 ± 0.152
3.572LysAsp: 3.572 ± 0.402
3.232LysGlu: 3.232 ± 0.323
2.212LysPhe: 2.212 ± 0.373
3.913LysGly: 3.913 ± 0.432
0.851LysHis: 0.851 ± 0.223
3.147LysIle: 3.147 ± 0.374
3.7LysLys: 3.7 ± 0.473
4.678LysLeu: 4.678 ± 0.442
1.574LysMet: 1.574 ± 0.217
2.892LysAsn: 2.892 ± 0.379
2.552LysPro: 2.552 ± 0.395
1.999LysGln: 1.999 ± 0.294
3.062LysArg: 3.062 ± 0.357
3.913LysSer: 3.913 ± 0.483
2.849LysThr: 2.849 ± 0.308
4.125LysVal: 4.125 ± 0.455
0.638LysTrp: 0.638 ± 0.142
1.786LysTyr: 1.786 ± 0.348
0.0LysXaa: 0.0 ± 0.0
Leu
6.762LeuAla: 6.762 ± 0.92
0.851LeuCys: 0.851 ± 0.25
5.784LeuAsp: 5.784 ± 0.487
6.124LeuGlu: 6.124 ± 0.562
2.339LeuPhe: 2.339 ± 0.285
6.337LeuGly: 6.337 ± 0.565
0.978LeuHis: 0.978 ± 0.225
3.487LeuIle: 3.487 ± 0.42
4.253LeuLys: 4.253 ± 0.555
5.359LeuLeu: 5.359 ± 0.523
1.914LeuMet: 1.914 ± 0.266
3.19LeuAsn: 3.19 ± 0.359
4.295LeuPro: 4.295 ± 0.373
2.424LeuGln: 2.424 ± 0.357
4.678LeuArg: 4.678 ± 0.502
4.848LeuSer: 4.848 ± 0.406
4.466LeuThr: 4.466 ± 0.441
4.551LeuVal: 4.551 ± 0.471
1.318LeuTrp: 1.318 ± 0.191
2.254LeuTyr: 2.254 ± 0.333
0.0LeuXaa: 0.0 ± 0.0
Met
2.552MetAla: 2.552 ± 0.355
0.128MetCys: 0.128 ± 0.076
1.914MetAsp: 1.914 ± 0.288
1.446MetGlu: 1.446 ± 0.251
1.063MetPhe: 1.063 ± 0.208
2.084MetGly: 2.084 ± 0.383
0.808MetHis: 0.808 ± 0.208
1.403MetIle: 1.403 ± 0.226
1.531MetLys: 1.531 ± 0.256
2.126MetLeu: 2.126 ± 0.294
0.553MetMet: 0.553 ± 0.142
0.936MetAsn: 0.936 ± 0.205
1.744MetPro: 1.744 ± 0.3
1.063MetGln: 1.063 ± 0.241
2.169MetArg: 2.169 ± 0.375
2.382MetSer: 2.382 ± 0.324
2.339MetThr: 2.339 ± 0.317
1.956MetVal: 1.956 ± 0.32
0.085MetTrp: 0.085 ± 0.058
0.808MetTyr: 0.808 ± 0.227
0.0MetXaa: 0.0 ± 0.0
Asn
3.615AsnAla: 3.615 ± 0.719
0.383AsnCys: 0.383 ± 0.166
2.297AsnAsp: 2.297 ± 0.262
2.637AsnGlu: 2.637 ± 0.365
1.744AsnPhe: 1.744 ± 0.283
4.083AsnGly: 4.083 ± 0.388
0.723AsnHis: 0.723 ± 0.201
2.892AsnIle: 2.892 ± 0.435
2.084AsnLys: 2.084 ± 0.355
3.913AsnLeu: 3.913 ± 0.369
1.106AsnMet: 1.106 ± 0.202
1.318AsnAsn: 1.318 ± 0.23
3.572AsnPro: 3.572 ± 0.302
1.999AsnGln: 1.999 ± 0.314
1.914AsnArg: 1.914 ± 0.349
2.169AsnSer: 2.169 ± 0.248
2.594AsnThr: 2.594 ± 0.396
2.552AsnVal: 2.552 ± 0.404
0.68AsnTrp: 0.68 ± 0.163
1.148AsnTyr: 1.148 ± 0.255
0.0AsnXaa: 0.0 ± 0.0
Pro
4.508ProAla: 4.508 ± 0.602
0.298ProCys: 0.298 ± 0.129
3.317ProAsp: 3.317 ± 0.463
3.913ProGlu: 3.913 ± 0.472
1.403ProPhe: 1.403 ± 0.203
4.083ProGly: 4.083 ± 0.395
1.106ProHis: 1.106 ± 0.232
2.297ProIle: 2.297 ± 0.287
2.382ProLys: 2.382 ± 0.467
3.19ProLeu: 3.19 ± 0.406
1.276ProMet: 1.276 ± 0.212
2.382ProAsn: 2.382 ± 0.297
1.446ProPro: 1.446 ± 0.248
2.126ProGln: 2.126 ± 0.296
2.084ProArg: 2.084 ± 0.332
3.53ProSer: 3.53 ± 0.336
2.467ProThr: 2.467 ± 0.402
3.19ProVal: 3.19 ± 0.408
1.106ProTrp: 1.106 ± 0.23
1.531ProTyr: 1.531 ± 0.341
0.0ProXaa: 0.0 ± 0.0
Gln
4.295GlnAla: 4.295 ± 0.408
0.128GlnCys: 0.128 ± 0.07
2.126GlnAsp: 2.126 ± 0.288
2.424GlnGlu: 2.424 ± 0.364
1.361GlnPhe: 1.361 ± 0.255
2.382GlnGly: 2.382 ± 0.396
0.51GlnHis: 0.51 ± 0.167
2.212GlnIle: 2.212 ± 0.308
2.467GlnLys: 2.467 ± 0.429
2.977GlnLeu: 2.977 ± 0.282
1.446GlnMet: 1.446 ± 0.194
1.701GlnAsn: 1.701 ± 0.211
2.084GlnPro: 2.084 ± 0.331
1.403GlnGln: 1.403 ± 0.318
1.786GlnArg: 1.786 ± 0.297
2.126GlnSer: 2.126 ± 0.313
2.382GlnThr: 2.382 ± 0.317
2.382GlnVal: 2.382 ± 0.409
0.468GlnTrp: 0.468 ± 0.128
1.446GlnTyr: 1.446 ± 0.271
0.0GlnXaa: 0.0 ± 0.0
Arg
5.146ArgAla: 5.146 ± 0.73
0.68ArgCys: 0.68 ± 0.173
3.572ArgAsp: 3.572 ± 0.527
3.828ArgGlu: 3.828 ± 0.532
1.914ArgPhe: 1.914 ± 0.226
3.02ArgGly: 3.02 ± 0.291
1.446ArgHis: 1.446 ± 0.314
3.658ArgIle: 3.658 ± 0.578
3.36ArgLys: 3.36 ± 0.454
3.785ArgLeu: 3.785 ± 0.473
1.744ArgMet: 1.744 ± 0.272
2.254ArgAsn: 2.254 ± 0.309
2.467ArgPro: 2.467 ± 0.364
1.871ArgGln: 1.871 ± 0.327
4.168ArgArg: 4.168 ± 0.381
2.722ArgSer: 2.722 ± 0.387
2.424ArgThr: 2.424 ± 0.326
4.593ArgVal: 4.593 ± 0.46
0.893ArgTrp: 0.893 ± 0.21
1.403ArgTyr: 1.403 ± 0.252
0.0ArgXaa: 0.0 ± 0.0
Ser
4.976SerAla: 4.976 ± 0.587
0.595SerCys: 0.595 ± 0.191
3.7SerAsp: 3.7 ± 0.42
3.317SerGlu: 3.317 ± 0.374
2.637SerPhe: 2.637 ± 0.284
5.444SerGly: 5.444 ± 0.582
0.936SerHis: 0.936 ± 0.188
3.87SerIle: 3.87 ± 0.343
3.232SerLys: 3.232 ± 0.405
4.168SerLeu: 4.168 ± 0.432
2.169SerMet: 2.169 ± 0.372
2.849SerAsn: 2.849 ± 0.312
2.892SerPro: 2.892 ± 0.35
1.914SerGln: 1.914 ± 0.357
3.53SerArg: 3.53 ± 0.351
4.593SerSer: 4.593 ± 0.669
3.572SerThr: 3.572 ± 0.374
4.253SerVal: 4.253 ± 0.508
0.978SerTrp: 0.978 ± 0.175
2.424SerTyr: 2.424 ± 0.353
0.0SerXaa: 0.0 ± 0.0
Thr
5.486ThrAla: 5.486 ± 0.796
0.468ThrCys: 0.468 ± 0.138
3.658ThrAsp: 3.658 ± 0.442
4.168ThrGlu: 4.168 ± 0.407
2.424ThrPhe: 2.424 ± 0.342
5.529ThrGly: 5.529 ± 0.525
1.446ThrHis: 1.446 ± 0.255
2.892ThrIle: 2.892 ± 0.372
3.36ThrLys: 3.36 ± 0.353
4.848ThrLeu: 4.848 ± 0.499
1.446ThrMet: 1.446 ± 0.203
2.807ThrAsn: 2.807 ± 0.361
3.275ThrPro: 3.275 ± 0.6
2.764ThrGln: 2.764 ± 0.323
3.19ThrArg: 3.19 ± 0.346
3.658ThrSer: 3.658 ± 0.528
3.828ThrThr: 3.828 ± 0.439
4.125ThrVal: 4.125 ± 0.385
0.723ThrTrp: 0.723 ± 0.207
2.424ThrTyr: 2.424 ± 0.312
0.0ThrXaa: 0.0 ± 0.0
Val
6.762ValAla: 6.762 ± 0.537
0.595ValCys: 0.595 ± 0.188
3.743ValAsp: 3.743 ± 0.312
4.593ValGlu: 4.593 ± 0.434
2.212ValPhe: 2.212 ± 0.388
4.253ValGly: 4.253 ± 0.471
1.403ValHis: 1.403 ± 0.245
3.02ValIle: 3.02 ± 0.361
3.36ValLys: 3.36 ± 0.397
4.848ValLeu: 4.848 ± 0.433
2.297ValMet: 2.297 ± 0.348
3.062ValAsn: 3.062 ± 0.304
3.105ValPro: 3.105 ± 0.323
2.594ValGln: 2.594 ± 0.308
3.232ValArg: 3.232 ± 0.519
4.04ValSer: 4.04 ± 0.42
5.444ValThr: 5.444 ± 0.535
4.338ValVal: 4.338 ± 0.409
0.638ValTrp: 0.638 ± 0.134
1.871ValTyr: 1.871 ± 0.302
0.0ValXaa: 0.0 ± 0.0
Trp
1.403TrpAla: 1.403 ± 0.237
0.34TrpCys: 0.34 ± 0.123
1.148TrpAsp: 1.148 ± 0.239
1.191TrpGlu: 1.191 ± 0.242
0.383TrpPhe: 0.383 ± 0.135
1.446TrpGly: 1.446 ± 0.238
0.425TrpHis: 0.425 ± 0.155
0.978TrpIle: 0.978 ± 0.192
1.106TrpLys: 1.106 ± 0.261
1.574TrpLeu: 1.574 ± 0.242
0.468TrpMet: 0.468 ± 0.139
1.148TrpAsn: 1.148 ± 0.201
0.34TrpPro: 0.34 ± 0.108
0.468TrpGln: 0.468 ± 0.145
0.893TrpArg: 0.893 ± 0.187
1.106TrpSer: 1.106 ± 0.208
0.553TrpThr: 0.553 ± 0.146
0.893TrpVal: 0.893 ± 0.199
0.17TrpTrp: 0.17 ± 0.081
0.383TrpTyr: 0.383 ± 0.12
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.467TyrAla: 2.467 ± 0.297
0.425TyrCys: 0.425 ± 0.136
2.254TyrAsp: 2.254 ± 0.322
1.744TyrGlu: 1.744 ± 0.277
1.021TyrPhe: 1.021 ± 0.186
2.722TyrGly: 2.722 ± 0.326
0.766TyrHis: 0.766 ± 0.18
1.403TyrIle: 1.403 ± 0.23
1.744TyrLys: 1.744 ± 0.321
2.212TyrLeu: 2.212 ± 0.268
0.68TyrMet: 0.68 ± 0.171
1.446TyrAsn: 1.446 ± 0.253
1.318TyrPro: 1.318 ± 0.292
1.361TyrGln: 1.361 ± 0.248
1.871TyrArg: 1.871 ± 0.392
2.041TyrSer: 2.041 ± 0.298
2.126TyrThr: 2.126 ± 0.376
1.956TyrVal: 1.956 ± 0.308
0.298TyrTrp: 0.298 ± 0.108
1.106TyrTyr: 1.106 ± 0.251
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 105 proteins (23514 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski