Pseudomonas phage phi2954

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Vidaverviricetes; Mindivirales; Cystoviridae; Cystovirus; Pseudomonas virus phi2954

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C0KIT5|C0KIT5_9VIRU p15 OS=Pseudomonas phage phi2954 OX=593131 PE=4 SV=1
MM1 pKa = 7.53LKK3 pKa = 9.59VQCYY7 pKa = 7.69EE8 pKa = 3.9CGVIFNYY15 pKa = 10.54YY16 pKa = 10.4NEE18 pKa = 5.31DD19 pKa = 3.96DD20 pKa = 4.8NPPCDD25 pKa = 5.22SEE27 pKa = 4.75TCDD30 pKa = 3.1ACLDD34 pKa = 3.59KK35 pKa = 11.34FEE37 pKa = 4.72EE38 pKa = 4.23EE39 pKa = 5.03RR40 pKa = 11.84ITEE43 pKa = 3.82EE44 pKa = 4.95DD45 pKa = 3.59GCLKK49 pKa = 10.83

Molecular weight: 5.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C0KIU2|C0KIU2_9VIRU p6 OS=Pseudomonas phage phi2954 OX=593131 PE=4 SV=1
MM1 pKa = 6.95AHH3 pKa = 6.53ARR5 pKa = 11.84ISTSTRR11 pKa = 11.84PTFSRR16 pKa = 11.84SLDD19 pKa = 3.47VAHH22 pKa = 6.51QSSGFHH28 pKa = 6.85DD29 pKa = 3.82RR30 pKa = 11.84LFVAGVRR37 pKa = 11.84MWNHH41 pKa = 5.67EE42 pKa = 4.17HH43 pKa = 6.86TSMQRR48 pKa = 11.84ALRR51 pKa = 11.84RR52 pKa = 11.84LHH54 pKa = 6.2LASSRR59 pKa = 11.84VINFSVFLLLGSGAGTLLMM78 pKa = 5.42

Molecular weight: 8.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14

0

14

3542

42

711

253.0

27.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.277 ± 0.677

0.903 ± 0.208

6.126 ± 0.434

5.336 ± 0.39

4.178 ± 0.301

7.51 ± 0.556

1.722 ± 0.425

5.167 ± 0.478

5.195 ± 0.315

8.498 ± 0.274

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.331 ± 0.265

3.727 ± 0.374

3.981 ± 0.364

3.586 ± 0.311

5.082 ± 0.304

6.889 ± 0.539

6.494 ± 0.509

8.187 ± 0.382

1.101 ± 0.248

2.71 ± 0.323
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski