Amino acid dipepetide frequency for Pseudomonas phage phi2954

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.884AlaAla: 9.884 ± 2.29
1.13AlaCys: 1.13 ± 0.458
5.366AlaAsp: 5.366 ± 1.335
3.106AlaGlu: 3.106 ± 0.637
5.648AlaPhe: 5.648 ± 0.975
7.343AlaGly: 7.343 ± 1.156
1.412AlaHis: 1.412 ± 0.801
5.083AlaIle: 5.083 ± 0.887
7.625AlaLys: 7.625 ± 1.188
7.907AlaLeu: 7.907 ± 1.974
3.389AlaMet: 3.389 ± 1.005
3.671AlaAsn: 3.671 ± 0.661
2.259AlaPro: 2.259 ± 0.562
4.236AlaGln: 4.236 ± 1.462
6.778AlaArg: 6.778 ± 1.43
5.931AlaSer: 5.931 ± 1.386
8.19AlaThr: 8.19 ± 1.296
9.602AlaVal: 9.602 ± 2.112
0.565AlaTrp: 0.565 ± 0.346
3.389AlaTyr: 3.389 ± 0.751
0.0AlaXaa: 0.0 ± 0.0
Cys
0.0CysAla: 0.0 ± 0.0
0.0CysCys: 0.0 ± 0.0
0.847CysAsp: 0.847 ± 0.716
0.847CysGlu: 0.847 ± 0.473
0.0CysPhe: 0.0 ± 0.0
1.13CysGly: 1.13 ± 0.406
0.0CysHis: 0.0 ± 0.0
0.282CysIle: 0.282 ± 0.285
0.282CysLys: 0.282 ± 0.342
1.13CysLeu: 1.13 ± 0.618
0.0CysMet: 0.0 ± 0.0
0.282CysAsn: 0.282 ± 0.24
0.565CysPro: 0.565 ± 0.272
0.0CysGln: 0.0 ± 0.0
0.565CysArg: 0.565 ± 0.417
0.565CysSer: 0.565 ± 0.417
0.282CysThr: 0.282 ± 0.24
1.412CysVal: 1.412 ± 0.752
0.282CysTrp: 0.282 ± 0.24
0.565CysTyr: 0.565 ± 0.359
0.0CysXaa: 0.0 ± 0.0
Asp
5.931AspAla: 5.931 ± 0.941
0.565AspCys: 0.565 ± 0.272
3.389AspAsp: 3.389 ± 0.591
3.671AspGlu: 3.671 ± 1.012
3.671AspPhe: 3.671 ± 0.813
3.106AspGly: 3.106 ± 0.793
0.847AspHis: 0.847 ± 0.427
2.824AspIle: 2.824 ± 0.666
3.954AspLys: 3.954 ± 0.681
5.366AspLeu: 5.366 ± 0.818
2.542AspMet: 2.542 ± 0.804
1.694AspAsn: 1.694 ± 0.781
4.801AspPro: 4.801 ± 1.355
2.542AspGln: 2.542 ± 0.985
3.954AspArg: 3.954 ± 1.258
3.106AspSer: 3.106 ± 1.048
3.106AspThr: 3.106 ± 0.866
4.518AspVal: 4.518 ± 1.532
0.0AspTrp: 0.0 ± 0.0
1.694AspTyr: 1.694 ± 0.614
0.0AspXaa: 0.0 ± 0.0
Glu
4.801GluAla: 4.801 ± 1.121
0.282GluCys: 0.282 ± 0.325
3.389GluAsp: 3.389 ± 0.84
3.954GluGlu: 3.954 ± 1.915
2.259GluPhe: 2.259 ± 0.425
2.259GluGly: 2.259 ± 1.095
0.847GluHis: 0.847 ± 0.753
4.236GluIle: 4.236 ± 1.161
1.977GluLys: 1.977 ± 1.094
4.236GluLeu: 4.236 ± 1.217
1.412GluMet: 1.412 ± 0.651
1.412GluAsn: 1.412 ± 0.457
0.847GluPro: 0.847 ± 0.402
4.236GluGln: 4.236 ± 1.391
4.518GluArg: 4.518 ± 1.278
1.694GluSer: 1.694 ± 0.749
4.236GluThr: 4.236 ± 1.238
5.083GluVal: 5.083 ± 0.717
0.0GluTrp: 0.0 ± 0.0
1.694GluTyr: 1.694 ± 0.436
0.0GluXaa: 0.0 ± 0.0
Phe
5.083PheAla: 5.083 ± 1.115
0.282PheCys: 0.282 ± 0.23
3.671PheAsp: 3.671 ± 1.374
3.671PheGlu: 3.671 ± 0.956
2.259PhePhe: 2.259 ± 0.722
3.671PheGly: 3.671 ± 1.19
0.565PheHis: 0.565 ± 0.49
2.542PheIle: 2.542 ± 1.179
1.13PheLys: 1.13 ± 0.385
3.106PheLeu: 3.106 ± 0.986
1.13PheMet: 1.13 ± 0.394
2.259PheAsn: 2.259 ± 0.571
1.13PhePro: 1.13 ± 0.509
1.694PheGln: 1.694 ± 0.549
1.694PheArg: 1.694 ± 0.631
1.694PheSer: 1.694 ± 0.748
1.694PheThr: 1.694 ± 0.585
2.542PheVal: 2.542 ± 0.768
0.565PheTrp: 0.565 ± 0.357
1.412PheTyr: 1.412 ± 0.712
0.0PheXaa: 0.0 ± 0.0
Gly
6.213GlyAla: 6.213 ± 1.445
1.13GlyCys: 1.13 ± 0.643
4.518GlyAsp: 4.518 ± 1.507
5.366GlyGlu: 5.366 ± 2.125
2.824GlyPhe: 2.824 ± 1.035
3.671GlyGly: 3.671 ± 0.711
0.565GlyHis: 0.565 ± 0.35
1.977GlyIle: 1.977 ± 0.762
4.518GlyLys: 4.518 ± 0.897
9.319GlyLeu: 9.319 ± 1.56
3.106GlyMet: 3.106 ± 1.004
2.824GlyAsn: 2.824 ± 1.037
1.977GlyPro: 1.977 ± 0.729
1.694GlyGln: 1.694 ± 0.953
3.106GlyArg: 3.106 ± 0.908
5.931GlySer: 5.931 ± 0.737
5.648GlyThr: 5.648 ± 1.057
6.495GlyVal: 6.495 ± 1.147
1.694GlyTrp: 1.694 ± 0.621
2.542GlyTyr: 2.542 ± 0.593
0.0GlyXaa: 0.0 ± 0.0
His
1.694HisAla: 1.694 ± 0.587
0.0HisCys: 0.0 ± 0.0
0.282HisAsp: 0.282 ± 0.346
0.847HisGlu: 0.847 ± 0.507
0.565HisPhe: 0.565 ± 0.417
0.847HisGly: 0.847 ± 0.511
1.13HisHis: 1.13 ± 0.58
0.847HisIle: 0.847 ± 0.43
0.565HisLys: 0.565 ± 0.346
1.694HisLeu: 1.694 ± 0.969
0.0HisMet: 0.0 ± 0.0
0.565HisAsn: 0.565 ± 0.346
0.847HisPro: 0.847 ± 0.478
0.282HisGln: 0.282 ± 0.346
1.412HisArg: 1.412 ± 0.671
1.694HisSer: 1.694 ± 0.987
1.412HisThr: 1.412 ± 0.778
1.694HisVal: 1.694 ± 0.71
0.0HisTrp: 0.0 ± 0.0
0.847HisTyr: 0.847 ± 0.436
0.0HisXaa: 0.0 ± 0.0
Ile
9.037IleAla: 9.037 ± 1.125
0.0IleCys: 0.0 ± 0.0
3.954IleAsp: 3.954 ± 0.823
2.259IleGlu: 2.259 ± 0.753
2.259IlePhe: 2.259 ± 0.733
4.518IleGly: 4.518 ± 1.103
0.847IleHis: 0.847 ± 0.864
1.977IleIle: 1.977 ± 1.154
2.259IleLys: 2.259 ± 0.616
1.694IleLeu: 1.694 ± 0.73
1.13IleMet: 1.13 ± 0.386
3.106IleAsn: 3.106 ± 0.67
1.694IlePro: 1.694 ± 0.44
1.13IleGln: 1.13 ± 0.716
2.259IleArg: 2.259 ± 0.777
3.671IleSer: 3.671 ± 1.001
3.671IleThr: 3.671 ± 0.96
4.518IleVal: 4.518 ± 1.468
0.565IleTrp: 0.565 ± 0.393
1.13IleTyr: 1.13 ± 0.5
0.0IleXaa: 0.0 ± 0.0
Lys
5.366LysAla: 5.366 ± 1.462
0.282LysCys: 0.282 ± 0.307
4.236LysAsp: 4.236 ± 1.037
1.13LysGlu: 1.13 ± 0.752
2.824LysPhe: 2.824 ± 0.66
1.977LysGly: 1.977 ± 0.881
0.565LysHis: 0.565 ± 0.368
3.389LysIle: 3.389 ± 1.243
1.694LysLys: 1.694 ± 0.761
3.671LysLeu: 3.671 ± 0.638
1.977LysMet: 1.977 ± 0.432
2.542LysAsn: 2.542 ± 0.695
3.671LysPro: 3.671 ± 0.962
1.13LysGln: 1.13 ± 0.556
1.694LysArg: 1.694 ± 0.707
3.954LysSer: 3.954 ± 1.079
3.954LysThr: 3.954 ± 1.55
6.213LysVal: 6.213 ± 1.193
0.565LysTrp: 0.565 ± 0.475
1.13LysTyr: 1.13 ± 0.407
0.0LysXaa: 0.0 ± 0.0
Leu
9.602LeuAla: 9.602 ± 1.509
1.13LeuCys: 1.13 ± 0.638
5.083LeuAsp: 5.083 ± 1.003
4.236LeuGlu: 4.236 ± 0.785
2.824LeuPhe: 2.824 ± 0.713
8.19LeuGly: 8.19 ± 1.996
1.694LeuHis: 1.694 ± 0.776
5.083LeuIle: 5.083 ± 1.766
3.954LeuLys: 3.954 ± 1.126
9.319LeuLeu: 9.319 ± 2.112
3.671LeuMet: 3.671 ± 0.851
3.954LeuAsn: 3.954 ± 1.108
4.518LeuPro: 4.518 ± 0.853
2.824LeuGln: 2.824 ± 0.889
2.824LeuArg: 2.824 ± 0.786
5.366LeuSer: 5.366 ± 1.208
5.083LeuThr: 5.083 ± 1.645
4.518LeuVal: 4.518 ± 0.922
0.0LeuTrp: 0.0 ± 0.0
1.13LeuTyr: 1.13 ± 0.441
0.0LeuXaa: 0.0 ± 0.0
Met
2.824MetAla: 2.824 ± 1.013
0.282MetCys: 0.282 ± 0.24
1.13MetAsp: 1.13 ± 0.717
0.847MetGlu: 0.847 ± 0.478
1.412MetPhe: 1.412 ± 0.545
3.106MetGly: 3.106 ± 0.776
0.565MetHis: 0.565 ± 0.29
1.977MetIle: 1.977 ± 0.5
1.694MetLys: 1.694 ± 0.598
1.694MetLeu: 1.694 ± 0.553
1.13MetMet: 1.13 ± 0.43
1.13MetAsn: 1.13 ± 0.621
2.259MetPro: 2.259 ± 0.723
1.694MetGln: 1.694 ± 0.461
1.694MetArg: 1.694 ± 0.604
2.542MetSer: 2.542 ± 0.621
2.542MetThr: 2.542 ± 0.862
3.671MetVal: 3.671 ± 0.703
0.565MetTrp: 0.565 ± 0.397
0.565MetTyr: 0.565 ± 0.272
0.0MetXaa: 0.0 ± 0.0
Asn
3.389AsnAla: 3.389 ± 1.187
0.847AsnCys: 0.847 ± 0.458
3.106AsnAsp: 3.106 ± 0.948
1.977AsnGlu: 1.977 ± 0.563
0.565AsnPhe: 0.565 ± 0.405
3.106AsnGly: 3.106 ± 0.642
0.282AsnHis: 0.282 ± 0.346
0.847AsnIle: 0.847 ± 0.365
2.259AsnLys: 2.259 ± 0.63
2.259AsnLeu: 2.259 ± 0.673
0.565AsnMet: 0.565 ± 0.29
0.565AsnAsn: 0.565 ± 0.272
3.106AsnPro: 3.106 ± 0.991
1.977AsnGln: 1.977 ± 0.742
1.412AsnArg: 1.412 ± 0.645
3.106AsnSer: 3.106 ± 0.775
3.671AsnThr: 3.671 ± 1.082
2.259AsnVal: 2.259 ± 0.645
0.565AsnTrp: 0.565 ± 0.417
1.412AsnTyr: 1.412 ± 0.523
0.0AsnXaa: 0.0 ± 0.0
Pro
3.106ProAla: 3.106 ± 1.181
1.13ProCys: 1.13 ± 0.541
2.824ProAsp: 2.824 ± 0.85
1.977ProGlu: 1.977 ± 0.804
0.847ProPhe: 0.847 ± 0.414
4.518ProGly: 4.518 ± 0.88
0.847ProHis: 0.847 ± 0.564
1.13ProIle: 1.13 ± 0.502
2.824ProLys: 2.824 ± 0.832
3.671ProLeu: 3.671 ± 1.098
0.282ProMet: 0.282 ± 0.238
0.565ProAsn: 0.565 ± 0.26
0.847ProPro: 0.847 ± 0.491
1.13ProGln: 1.13 ± 0.42
2.542ProArg: 2.542 ± 0.969
3.671ProSer: 3.671 ± 0.629
1.412ProThr: 1.412 ± 0.489
5.366ProVal: 5.366 ± 1.238
0.282ProTrp: 0.282 ± 0.23
0.847ProTyr: 0.847 ± 0.243
0.0ProXaa: 0.0 ± 0.0
Gln
3.671GlnAla: 3.671 ± 1.224
0.282GlnCys: 0.282 ± 0.325
1.977GlnAsp: 1.977 ± 1.01
1.977GlnGlu: 1.977 ± 1.037
1.694GlnPhe: 1.694 ± 0.754
1.977GlnGly: 1.977 ± 0.56
1.412GlnHis: 1.412 ± 0.525
0.565GlnIle: 0.565 ± 0.479
1.977GlnLys: 1.977 ± 0.837
2.259GlnLeu: 2.259 ± 0.474
1.977GlnMet: 1.977 ± 0.927
1.412GlnAsn: 1.412 ± 0.559
0.847GlnPro: 0.847 ± 0.362
0.847GlnGln: 0.847 ± 0.487
1.977GlnArg: 1.977 ± 0.977
3.389GlnSer: 3.389 ± 1.099
2.259GlnThr: 2.259 ± 0.58
3.671GlnVal: 3.671 ± 0.79
0.0GlnTrp: 0.0 ± 0.0
1.694GlnTyr: 1.694 ± 0.653
0.0GlnXaa: 0.0 ± 0.0
Arg
5.366ArgAla: 5.366 ± 1.229
0.565ArgCys: 0.565 ± 0.417
3.389ArgAsp: 3.389 ± 0.925
3.954ArgGlu: 3.954 ± 1.059
1.694ArgPhe: 1.694 ± 0.465
3.106ArgGly: 3.106 ± 0.806
0.0ArgHis: 0.0 ± 0.0
2.824ArgIle: 2.824 ± 0.991
1.412ArgLys: 1.412 ± 0.712
3.671ArgLeu: 3.671 ± 1.414
2.542ArgMet: 2.542 ± 0.837
1.977ArgAsn: 1.977 ± 0.797
2.542ArgPro: 2.542 ± 0.926
3.106ArgGln: 3.106 ± 1.159
1.977ArgArg: 1.977 ± 0.619
5.083ArgSer: 5.083 ± 0.945
2.542ArgThr: 2.542 ± 0.979
2.824ArgVal: 2.824 ± 1.021
1.412ArgTrp: 1.412 ± 0.714
0.847ArgTyr: 0.847 ± 0.679
0.0ArgXaa: 0.0 ± 0.0
Ser
7.06SerAla: 7.06 ± 1.211
0.282SerCys: 0.282 ± 0.307
4.801SerAsp: 4.801 ± 1.195
2.259SerGlu: 2.259 ± 0.908
2.542SerPhe: 2.542 ± 0.739
6.213SerGly: 6.213 ± 1.987
1.977SerHis: 1.977 ± 1.295
4.518SerIle: 4.518 ± 0.82
3.106SerLys: 3.106 ± 0.58
5.083SerLeu: 5.083 ± 1.433
2.824SerMet: 2.824 ± 0.854
1.412SerAsn: 1.412 ± 0.4
1.977SerPro: 1.977 ± 0.741
2.259SerGln: 2.259 ± 0.808
3.389SerArg: 3.389 ± 0.768
5.083SerSer: 5.083 ± 1.608
5.083SerThr: 5.083 ± 1.068
5.931SerVal: 5.931 ± 1.015
1.412SerTrp: 1.412 ± 0.611
1.694SerTyr: 1.694 ± 0.762
0.0SerXaa: 0.0 ± 0.0
Thr
5.931ThrAla: 5.931 ± 1.203
0.282ThrCys: 0.282 ± 0.325
2.824ThrAsp: 2.824 ± 0.94
4.236ThrGlu: 4.236 ± 0.816
3.389ThrPhe: 3.389 ± 0.743
5.648ThrGly: 5.648 ± 1.129
1.412ThrHis: 1.412 ± 0.567
4.236ThrIle: 4.236 ± 1.36
4.236ThrLys: 4.236 ± 1.284
6.778ThrLeu: 6.778 ± 0.993
2.259ThrMet: 2.259 ± 0.796
2.824ThrAsn: 2.824 ± 1.175
1.977ThrPro: 1.977 ± 0.722
1.13ThrGln: 1.13 ± 0.457
2.542ThrArg: 2.542 ± 0.602
3.954ThrSer: 3.954 ± 1.172
4.518ThrThr: 4.518 ± 0.949
4.518ThrVal: 4.518 ± 0.954
0.282ThrTrp: 0.282 ± 0.23
1.977ThrTyr: 1.977 ± 0.512
0.0ThrXaa: 0.0 ± 0.0
Val
9.037ValAla: 9.037 ± 1.908
0.282ValCys: 0.282 ± 0.238
4.518ValAsp: 4.518 ± 1.324
5.931ValGlu: 5.931 ± 2.146
2.824ValPhe: 2.824 ± 0.749
7.06ValGly: 7.06 ± 1.069
1.412ValHis: 1.412 ± 0.674
6.213ValIle: 6.213 ± 0.888
4.236ValLys: 4.236 ± 0.943
8.755ValLeu: 8.755 ± 1.418
2.259ValMet: 2.259 ± 0.603
3.389ValAsn: 3.389 ± 0.712
1.412ValPro: 1.412 ± 0.573
1.977ValGln: 1.977 ± 0.982
3.954ValArg: 3.954 ± 0.882
6.495ValSer: 6.495 ± 1.275
4.236ValThr: 4.236 ± 1.148
5.366ValVal: 5.366 ± 1.734
1.694ValTrp: 1.694 ± 0.92
0.847ValTyr: 0.847 ± 0.52
0.0ValXaa: 0.0 ± 0.0
Trp
0.282TrpAla: 0.282 ± 0.23
0.282TrpCys: 0.282 ± 0.23
0.847TrpAsp: 0.847 ± 0.689
0.0TrpGlu: 0.0 ± 0.0
0.847TrpPhe: 0.847 ± 0.349
1.412TrpGly: 1.412 ± 0.51
0.282TrpHis: 0.282 ± 0.23
0.0TrpIle: 0.0 ± 0.0
0.847TrpLys: 0.847 ± 0.545
1.694TrpLeu: 1.694 ± 0.861
0.0TrpMet: 0.0 ± 0.0
0.847TrpAsn: 0.847 ± 0.43
0.282TrpPro: 0.282 ± 0.23
0.282TrpGln: 0.282 ± 0.23
0.565TrpArg: 0.565 ± 0.26
0.847TrpSer: 0.847 ± 0.243
0.847TrpThr: 0.847 ± 0.513
0.282TrpVal: 0.282 ± 0.24
0.0TrpTrp: 0.0 ± 0.0
0.565TrpTyr: 0.565 ± 0.402
0.0TrpXaa: 0.0 ± 0.0
Tyr
4.518TyrAla: 4.518 ± 1.173
0.0TyrCys: 0.0 ± 0.0
1.13TyrAsp: 1.13 ± 0.543
1.13TyrGlu: 1.13 ± 0.745
0.847TyrPhe: 0.847 ± 0.375
2.259TyrGly: 2.259 ± 0.648
0.565TyrHis: 0.565 ± 0.347
1.13TyrIle: 1.13 ± 0.534
1.412TyrLys: 1.412 ± 0.72
1.694TyrLeu: 1.694 ± 0.63
0.847TyrMet: 0.847 ± 0.44
0.847TyrAsn: 0.847 ± 0.464
2.259TyrPro: 2.259 ± 0.613
1.694TyrGln: 1.694 ± 0.527
1.977TyrArg: 1.977 ± 0.759
1.13TyrSer: 1.13 ± 0.394
0.565TyrThr: 0.565 ± 0.475
1.412TyrVal: 1.412 ± 0.786
0.565TyrTrp: 0.565 ± 0.272
1.13TyrTyr: 1.13 ± 0.51
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 14 proteins (3542 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski