Amino acid dipepetide frequency for Nosema ceranae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.293AlaAla: 1.293 ± 0.057
0.735AlaCys: 0.735 ± 0.025
1.559AlaAsp: 1.559 ± 0.044
1.895AlaGlu: 1.895 ± 0.054
1.826AlaPhe: 1.826 ± 0.047
1.219AlaGly: 1.219 ± 0.051
0.518AlaHis: 0.518 ± 0.025
2.771AlaIle: 2.771 ± 0.053
2.709AlaLys: 2.709 ± 0.063
3.486AlaLeu: 3.486 ± 0.073
0.596AlaMet: 0.596 ± 0.028
1.728AlaAsn: 1.728 ± 0.045
0.771AlaPro: 0.771 ± 0.039
0.856AlaGln: 0.856 ± 0.037
1.15AlaArg: 1.15 ± 0.041
2.213AlaSer: 2.213 ± 0.061
1.438AlaThr: 1.438 ± 0.051
1.758AlaVal: 1.758 ± 0.06
0.184AlaTrp: 0.184 ± 0.014
1.446AlaTyr: 1.446 ± 0.041
0.0AlaXaa: 0.0 ± 0.0
Cys
0.64CysAla: 0.64 ± 0.028
0.489CysCys: 0.489 ± 0.029
1.202CysAsp: 1.202 ± 0.04
1.206CysGlu: 1.206 ± 0.038
1.272CysPhe: 1.272 ± 0.041
0.935CysGly: 0.935 ± 0.037
0.306CysHis: 0.306 ± 0.019
2.152CysIle: 2.152 ± 0.059
2.335CysLys: 2.335 ± 0.056
2.018CysLeu: 2.018 ± 0.058
0.409CysMet: 0.409 ± 0.024
1.551CysAsn: 1.551 ± 0.045
0.507CysPro: 0.507 ± 0.03
0.466CysGln: 0.466 ± 0.022
0.737CysArg: 0.737 ± 0.032
1.377CysSer: 1.377 ± 0.04
1.194CysThr: 1.194 ± 0.042
1.075CysVal: 1.075 ± 0.037
0.128CysTrp: 0.128 ± 0.011
0.856CysTyr: 0.856 ± 0.039
0.0CysXaa: 0.0 ± 0.0
Asp
1.653AspAla: 1.653 ± 0.046
1.096AspCys: 1.096 ± 0.035
3.318AspAsp: 3.318 ± 0.073
3.956AspGlu: 3.956 ± 0.086
4.164AspPhe: 4.164 ± 0.079
1.794AspGly: 1.794 ± 0.05
0.678AspHis: 0.678 ± 0.03
6.22AspIle: 6.22 ± 0.09
5.689AspLys: 5.689 ± 0.102
6.434AspLeu: 6.434 ± 0.105
1.093AspMet: 1.093 ± 0.033
4.102AspAsn: 4.102 ± 0.084
1.239AspPro: 1.239 ± 0.042
1.224AspGln: 1.224 ± 0.04
1.617AspArg: 1.617 ± 0.047
3.527AspSer: 3.527 ± 0.078
2.902AspThr: 2.902 ± 0.07
3.306AspVal: 3.306 ± 0.07
0.342AspTrp: 0.342 ± 0.019
2.639AspTyr: 2.639 ± 0.064
0.0AspXaa: 0.0 ± 0.0
Glu
2.083GluAla: 2.083 ± 0.066
1.442GluCys: 1.442 ± 0.044
4.434GluAsp: 4.434 ± 0.085
5.885GluGlu: 5.885 ± 0.143
3.246GluPhe: 3.246 ± 0.064
2.242GluGly: 2.242 ± 0.052
1.226GluHis: 1.226 ± 0.042
7.276GluIle: 7.276 ± 0.114
7.219GluLys: 7.219 ± 0.132
5.665GluLeu: 5.665 ± 0.092
1.645GluMet: 1.645 ± 0.044
6.196GluAsn: 6.196 ± 0.122
1.242GluPro: 1.242 ± 0.041
1.924GluGln: 1.924 ± 0.051
2.289GluArg: 2.289 ± 0.053
4.12GluSer: 4.12 ± 0.091
2.907GluThr: 2.907 ± 0.067
3.206GluVal: 3.206 ± 0.06
0.406GluTrp: 0.406 ± 0.021
3.338GluTyr: 3.338 ± 0.063
0.0GluXaa: 0.0 ± 0.0
Phe
1.584PheAla: 1.584 ± 0.05
1.55PheCys: 1.55 ± 0.045
3.463PheAsp: 3.463 ± 0.066
3.463PheGlu: 3.463 ± 0.072
4.213PhePhe: 4.213 ± 0.092
2.066PheGly: 2.066 ± 0.056
0.773PheHis: 0.773 ± 0.03
6.165PheIle: 6.165 ± 0.114
5.831PheLys: 5.831 ± 0.092
6.801PheLeu: 6.801 ± 0.108
1.226PheMet: 1.226 ± 0.039
4.68PheAsn: 4.68 ± 0.08
1.197PhePro: 1.197 ± 0.036
1.222PheGln: 1.222 ± 0.038
1.837PheArg: 1.837 ± 0.048
4.427PheSer: 4.427 ± 0.087
3.304PheThr: 3.304 ± 0.073
3.267PheVal: 3.267 ± 0.061
0.405PheTrp: 0.405 ± 0.026
3.929PheTyr: 3.929 ± 0.075
0.0PheXaa: 0.0 ± 0.0
Gly
1.177GlyAla: 1.177 ± 0.054
0.806GlyCys: 0.806 ± 0.032
1.906GlyAsp: 1.906 ± 0.052
1.957GlyGlu: 1.957 ± 0.05
2.102GlyPhe: 2.102 ± 0.062
1.683GlyGly: 1.683 ± 0.067
0.783GlyHis: 0.783 ± 0.033
3.392GlyIle: 3.392 ± 0.076
3.265GlyLys: 3.265 ± 0.078
3.326GlyLeu: 3.326 ± 0.1
0.823GlyMet: 0.823 ± 0.035
2.062GlyAsn: 2.062 ± 0.053
0.742GlyPro: 0.742 ± 0.031
0.869GlyGln: 0.869 ± 0.039
1.441GlyArg: 1.441 ± 0.053
2.524GlySer: 2.524 ± 0.077
1.966GlyThr: 1.966 ± 0.053
2.086GlyVal: 2.086 ± 0.056
0.256GlyTrp: 0.256 ± 0.018
1.746GlyTyr: 1.746 ± 0.051
0.0GlyXaa: 0.0 ± 0.0
His
0.491HisAla: 0.491 ± 0.027
0.349HisCys: 0.349 ± 0.022
0.744HisAsp: 0.744 ± 0.028
1.0HisGlu: 1.0 ± 0.036
1.16HisPhe: 1.16 ± 0.037
0.603HisGly: 0.603 ± 0.027
0.306HisHis: 0.306 ± 0.021
1.413HisIle: 1.413 ± 0.049
1.945HisLys: 1.945 ± 0.047
1.729HisLeu: 1.729 ± 0.043
0.322HisMet: 0.322 ± 0.02
1.16HisAsn: 1.16 ± 0.038
0.549HisPro: 0.549 ± 0.03
0.409HisGln: 0.409 ± 0.022
0.669HisArg: 0.669 ± 0.031
1.291HisSer: 1.291 ± 0.039
0.845HisThr: 0.845 ± 0.032
0.902HisVal: 0.902 ± 0.03
0.101HisTrp: 0.101 ± 0.01
0.69HisTyr: 0.69 ± 0.029
0.0HisXaa: 0.0 ± 0.0
Ile
2.618IleAla: 2.618 ± 0.061
2.211IleCys: 2.211 ± 0.058
5.658IleAsp: 5.658 ± 0.075
6.331IleGlu: 6.331 ± 0.094
6.785IlePhe: 6.785 ± 0.107
3.059IleGly: 3.059 ± 0.069
1.567IleHis: 1.567 ± 0.042
8.321IleIle: 8.321 ± 0.116
10.371IleLys: 10.371 ± 0.131
10.54IleLeu: 10.54 ± 0.123
1.549IleMet: 1.549 ± 0.046
8.053IleAsn: 8.053 ± 0.119
2.572IlePro: 2.572 ± 0.056
2.633IleGln: 2.633 ± 0.052
3.147IleArg: 3.147 ± 0.07
7.068IleSer: 7.068 ± 0.086
4.261IleThr: 4.261 ± 0.073
4.508IleVal: 4.508 ± 0.075
0.491IleTrp: 0.491 ± 0.026
4.897IleTyr: 4.897 ± 0.087
0.0IleXaa: 0.0 ± 0.0
Lys
2.889LysAla: 2.889 ± 0.074
1.909LysCys: 1.909 ± 0.044
6.165LysAsp: 6.165 ± 0.109
7.876LysGlu: 7.876 ± 0.133
5.164LysPhe: 5.164 ± 0.087
3.178LysGly: 3.178 ± 0.071
1.919LysHis: 1.919 ± 0.045
11.139LysIle: 11.139 ± 0.128
12.483LysLys: 12.483 ± 0.184
8.935LysLeu: 8.935 ± 0.127
2.763LysMet: 2.763 ± 0.052
10.085LysAsn: 10.085 ± 0.148
2.062LysPro: 2.062 ± 0.056
2.942LysGln: 2.942 ± 0.07
3.88LysArg: 3.88 ± 0.076
6.943LysSer: 6.943 ± 0.123
4.759LysThr: 4.759 ± 0.082
4.481LysVal: 4.481 ± 0.07
0.549LysTrp: 0.549 ± 0.028
5.475LysTyr: 5.475 ± 0.095
0.0LysXaa: 0.0 ± 0.0
Leu
2.99LeuAla: 2.99 ± 0.068
2.122LeuCys: 2.122 ± 0.053
5.58LeuAsp: 5.58 ± 0.087
7.158LeuGlu: 7.158 ± 0.12
6.055LeuPhe: 6.055 ± 0.107
3.27LeuGly: 3.27 ± 0.073
1.823LeuHis: 1.823 ± 0.05
8.944LeuIle: 8.944 ± 0.132
10.524LeuLys: 10.524 ± 0.125
10.176LeuLeu: 10.176 ± 0.151
1.9LeuMet: 1.9 ± 0.048
7.566LeuAsn: 7.566 ± 0.109
2.517LeuPro: 2.517 ± 0.053
3.398LeuGln: 3.398 ± 0.073
3.456LeuArg: 3.456 ± 0.073
7.376LeuSer: 7.376 ± 0.11
4.246LeuThr: 4.246 ± 0.067
4.976LeuVal: 4.976 ± 0.083
0.526LeuTrp: 0.526 ± 0.025
4.889LeuTyr: 4.889 ± 0.098
0.0LeuXaa: 0.0 ± 0.0
Met
0.823MetAla: 0.823 ± 0.033
0.468MetCys: 0.468 ± 0.024
1.019MetAsp: 1.019 ± 0.037
1.137MetGlu: 1.137 ± 0.043
1.352MetPhe: 1.352 ± 0.041
0.78MetGly: 0.78 ± 0.037
0.411MetHis: 0.411 ± 0.023
1.716MetIle: 1.716 ± 0.036
2.046MetLys: 2.046 ± 0.047
1.97MetLeu: 1.97 ± 0.046
0.45MetMet: 0.45 ± 0.023
1.547MetAsn: 1.547 ± 0.045
0.629MetPro: 0.629 ± 0.024
0.567MetGln: 0.567 ± 0.028
0.73MetArg: 0.73 ± 0.029
1.532MetSer: 1.532 ± 0.045
1.01MetThr: 1.01 ± 0.036
0.993MetVal: 0.993 ± 0.037
0.152MetTrp: 0.152 ± 0.013
1.051MetTyr: 1.051 ± 0.035
0.0MetXaa: 0.0 ± 0.0
Asn
2.074AsnAla: 2.074 ± 0.045
1.362AsnCys: 1.362 ± 0.043
4.386AsnAsp: 4.386 ± 0.081
5.414AsnGlu: 5.414 ± 0.102
5.101AsnPhe: 5.101 ± 0.098
2.483AsnGly: 2.483 ± 0.083
1.037AsnHis: 1.037 ± 0.036
9.152AsnIle: 9.152 ± 0.106
8.45AsnLys: 8.45 ± 0.131
8.476AsnLeu: 8.476 ± 0.122
1.747AsnMet: 1.747 ± 0.044
6.935AsnAsn: 6.935 ± 0.136
1.736AsnPro: 1.736 ± 0.056
1.962AsnGln: 1.962 ± 0.058
2.385AsnArg: 2.385 ± 0.061
4.88AsnSer: 4.88 ± 0.081
4.083AsnThr: 4.083 ± 0.089
4.105AsnVal: 4.105 ± 0.076
0.401AsnTrp: 0.401 ± 0.021
3.504AsnTyr: 3.504 ± 0.076
0.0AsnXaa: 0.0 ± 0.0
Pro
0.79ProAla: 0.79 ± 0.04
0.457ProCys: 0.457 ± 0.026
1.275ProAsp: 1.275 ± 0.044
1.608ProGlu: 1.608 ± 0.056
1.578ProPhe: 1.578 ± 0.045
0.971ProGly: 0.971 ± 0.05
0.433ProHis: 0.433 ± 0.025
2.046ProIle: 2.046 ± 0.062
2.047ProLys: 2.047 ± 0.053
2.346ProLeu: 2.346 ± 0.058
0.453ProMet: 0.453 ± 0.027
1.622ProAsn: 1.622 ± 0.048
0.762ProPro: 0.762 ± 0.045
0.789ProGln: 0.789 ± 0.037
0.878ProArg: 0.878 ± 0.034
1.944ProSer: 1.944 ± 0.071
1.16ProThr: 1.16 ± 0.047
1.562ProVal: 1.562 ± 0.051
0.119ProTrp: 0.119 ± 0.012
1.057ProTyr: 1.057 ± 0.038
0.0ProXaa: 0.0 ± 0.0
Gln
0.898GlnAla: 0.898 ± 0.035
0.471GlnCys: 0.471 ± 0.027
1.472GlnAsp: 1.472 ± 0.041
2.074GlnGlu: 2.074 ± 0.054
1.309GlnPhe: 1.309 ± 0.034
0.878GlnGly: 0.878 ± 0.04
0.475GlnHis: 0.475 ± 0.025
2.673GlnIle: 2.673 ± 0.061
3.095GlnLys: 3.095 ± 0.075
2.123GlnLeu: 2.123 ± 0.051
0.64GlnMet: 0.64 ± 0.029
2.499GlnAsn: 2.499 ± 0.061
0.704GlnPro: 0.704 ± 0.041
0.983GlnGln: 0.983 ± 0.042
1.004GlnArg: 1.004 ± 0.037
1.784GlnSer: 1.784 ± 0.057
1.266GlnThr: 1.266 ± 0.038
1.293GlnVal: 1.293 ± 0.039
0.161GlnTrp: 0.161 ± 0.015
1.298GlnTyr: 1.298 ± 0.039
0.0GlnXaa: 0.0 ± 0.0
Arg
1.125ArgAla: 1.125 ± 0.036
0.753ArgCys: 0.753 ± 0.03
1.697ArgAsp: 1.697 ± 0.042
2.227ArgGlu: 2.227 ± 0.063
1.96ArgPhe: 1.96 ± 0.053
1.294ArgGly: 1.294 ± 0.042
0.766ArgHis: 0.766 ± 0.031
3.265ArgIle: 3.265 ± 0.071
3.726ArgLys: 3.726 ± 0.073
3.18ArgLeu: 3.18 ± 0.066
0.847ArgMet: 0.847 ± 0.037
2.675ArgAsn: 2.675 ± 0.053
0.877ArgPro: 0.877 ± 0.043
0.982ArgGln: 0.982 ± 0.033
1.779ArgArg: 1.779 ± 0.063
2.335ArgSer: 2.335 ± 0.06
1.562ArgThr: 1.562 ± 0.042
1.603ArgVal: 1.603 ± 0.051
0.231ArgTrp: 0.231 ± 0.018
1.603ArgTyr: 1.603 ± 0.05
0.0ArgXaa: 0.0 ± 0.0
Ser
2.227SerAla: 2.227 ± 0.057
1.336SerCys: 1.336 ± 0.049
3.991SerAsp: 3.991 ± 0.075
4.509SerGlu: 4.509 ± 0.085
4.246SerPhe: 4.246 ± 0.086
2.549SerGly: 2.549 ± 0.067
1.174SerHis: 1.174 ± 0.039
6.304SerIle: 6.304 ± 0.092
7.23SerLys: 7.23 ± 0.113
7.184SerLeu: 7.184 ± 0.114
1.212SerMet: 1.212 ± 0.04
5.196SerAsn: 5.196 ± 0.106
1.685SerPro: 1.685 ± 0.08
1.861SerGln: 1.861 ± 0.052
2.371SerArg: 2.371 ± 0.057
5.706SerSer: 5.706 ± 0.117
3.898SerThr: 3.898 ± 0.084
3.778SerVal: 3.778 ± 0.076
0.402SerTrp: 0.402 ± 0.022
3.134SerTyr: 3.134 ± 0.076
0.0SerXaa: 0.0 ± 0.0
Thr
1.621ThrAla: 1.621 ± 0.056
0.916ThrCys: 0.916 ± 0.039
2.771ThrAsp: 2.771 ± 0.064
3.298ThrGlu: 3.298 ± 0.076
3.011ThrPhe: 3.011 ± 0.058
2.108ThrGly: 2.108 ± 0.085
0.795ThrHis: 0.795 ± 0.034
4.199ThrIle: 4.199 ± 0.072
4.887ThrLys: 4.887 ± 0.078
4.517ThrLeu: 4.517 ± 0.067
0.744ThrMet: 0.744 ± 0.032
3.835ThrAsn: 3.835 ± 0.081
1.446ThrPro: 1.446 ± 0.047
1.321ThrGln: 1.321 ± 0.041
1.626ThrArg: 1.626 ± 0.045
3.715ThrSer: 3.715 ± 0.079
2.566ThrThr: 2.566 ± 0.075
2.691ThrVal: 2.691 ± 0.066
0.274ThrTrp: 0.274 ± 0.02
1.978ThrTyr: 1.978 ± 0.046
0.0ThrXaa: 0.0 ± 0.0
Val
1.571ValAla: 1.571 ± 0.05
1.198ValCys: 1.198 ± 0.041
3.195ValAsp: 3.195 ± 0.062
3.585ValGlu: 3.585 ± 0.073
3.195ValPhe: 3.195 ± 0.068
1.952ValGly: 1.952 ± 0.06
0.887ValHis: 0.887 ± 0.036
4.455ValIle: 4.455 ± 0.088
5.118ValLys: 5.118 ± 0.084
5.118ValLeu: 5.118 ± 0.072
0.907ValMet: 0.907 ± 0.034
3.696ValAsn: 3.696 ± 0.066
1.452ValPro: 1.452 ± 0.044
1.444ValGln: 1.444 ± 0.038
1.621ValArg: 1.621 ± 0.048
3.555ValSer: 3.555 ± 0.07
2.243ValThr: 2.243 ± 0.055
2.944ValVal: 2.944 ± 0.062
0.373ValTrp: 0.373 ± 0.021
2.571ValTyr: 2.571 ± 0.06
0.0ValXaa: 0.0 ± 0.0
Trp
0.229TrpAla: 0.229 ± 0.017
0.145TrpCys: 0.145 ± 0.014
0.357TrpAsp: 0.357 ± 0.02
0.342TrpGlu: 0.342 ± 0.022
0.354TrpPhe: 0.354 ± 0.021
0.213TrpGly: 0.213 ± 0.015
0.104TrpHis: 0.104 ± 0.013
0.564TrpIle: 0.564 ± 0.029
0.558TrpLys: 0.558 ± 0.028
0.521TrpLeu: 0.521 ± 0.026
0.157TrpMet: 0.157 ± 0.012
0.484TrpAsn: 0.484 ± 0.027
0.143TrpPro: 0.143 ± 0.014
0.122TrpGln: 0.122 ± 0.013
0.273TrpArg: 0.273 ± 0.017
0.405TrpSer: 0.405 ± 0.026
0.268TrpThr: 0.268 ± 0.017
0.267TrpVal: 0.267 ± 0.018
0.049TrpTrp: 0.049 ± 0.008
0.275TrpTyr: 0.275 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.374TyrAla: 1.374 ± 0.041
1.039TyrCys: 1.039 ± 0.035
2.801TyrAsp: 2.801 ± 0.064
3.068TyrGlu: 3.068 ± 0.064
3.31TyrPhe: 3.31 ± 0.068
1.631TyrGly: 1.631 ± 0.043
0.696TyrHis: 0.696 ± 0.032
4.473TyrIle: 4.473 ± 0.074
6.066TyrLys: 6.066 ± 0.096
4.947TyrLeu: 4.947 ± 0.09
0.903TyrMet: 0.903 ± 0.036
4.001TyrAsn: 4.001 ± 0.084
1.069TyrPro: 1.069 ± 0.038
1.124TyrGln: 1.124 ± 0.044
1.597TyrArg: 1.597 ± 0.046
3.271TyrSer: 3.271 ± 0.061
2.443TyrThr: 2.443 ± 0.057
2.281TyrVal: 2.281 ± 0.056
0.281TyrTrp: 0.281 ± 0.018
2.157TyrTyr: 2.157 ± 0.058
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3200 proteins (832824 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski