Amino acid dipepetide frequency for Muribaculum intestinale

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.683AlaAla: 7.683 ± 0.12
1.034AlaCys: 1.034 ± 0.035
5.873AlaAsp: 5.873 ± 0.093
4.9AlaGlu: 4.9 ± 0.072
3.112AlaPhe: 3.112 ± 0.075
6.084AlaGly: 6.084 ± 0.108
1.402AlaHis: 1.402 ± 0.037
5.743AlaIle: 5.743 ± 0.1
3.712AlaLys: 3.712 ± 0.067
7.435AlaLeu: 7.435 ± 0.096
2.745AlaMet: 2.745 ± 0.065
3.228AlaAsn: 3.228 ± 0.081
3.217AlaPro: 3.217 ± 0.061
2.454AlaGln: 2.454 ± 0.054
4.349AlaArg: 4.349 ± 0.079
5.379AlaSer: 5.379 ± 0.081
5.048AlaThr: 5.048 ± 0.08
5.955AlaVal: 5.955 ± 0.093
0.972AlaTrp: 0.972 ± 0.035
2.887AlaTyr: 2.887 ± 0.061
0.0AlaXaa: 0.0 ± 0.0
Cys
0.969CysAla: 0.969 ± 0.035
0.201CysCys: 0.201 ± 0.014
0.761CysAsp: 0.761 ± 0.031
0.64CysGlu: 0.64 ± 0.022
0.51CysPhe: 0.51 ± 0.026
1.196CysGly: 1.196 ± 0.041
0.342CysHis: 0.342 ± 0.019
0.804CysIle: 0.804 ± 0.028
0.503CysLys: 0.503 ± 0.026
0.904CysLeu: 0.904 ± 0.03
0.325CysMet: 0.325 ± 0.021
0.628CysAsn: 0.628 ± 0.028
0.584CysPro: 0.584 ± 0.026
0.318CysGln: 0.318 ± 0.02
0.867CysArg: 0.867 ± 0.029
0.816CysSer: 0.816 ± 0.027
0.675CysThr: 0.675 ± 0.029
0.869CysVal: 0.869 ± 0.032
0.15CysTrp: 0.15 ± 0.014
0.518CysTyr: 0.518 ± 0.027
0.0CysXaa: 0.0 ± 0.0
Asp
4.985AspAla: 4.985 ± 0.081
0.756AspCys: 0.756 ± 0.033
3.575AspAsp: 3.575 ± 0.068
3.688AspGlu: 3.688 ± 0.066
3.016AspPhe: 3.016 ± 0.058
5.108AspGly: 5.108 ± 0.088
0.979AspHis: 0.979 ± 0.038
4.981AspIle: 4.981 ± 0.073
3.202AspLys: 3.202 ± 0.071
4.479AspLeu: 4.479 ± 0.071
2.106AspMet: 2.106 ± 0.051
3.21AspAsn: 3.21 ± 0.063
2.211AspPro: 2.211 ± 0.052
1.122AspGln: 1.122 ± 0.037
3.31AspArg: 3.31 ± 0.057
4.066AspSer: 4.066 ± 0.074
3.579AspThr: 3.579 ± 0.065
3.833AspVal: 3.833 ± 0.068
0.844AspTrp: 0.844 ± 0.031
2.97AspTyr: 2.97 ± 0.062
0.0AspXaa: 0.0 ± 0.0
Glu
4.957GluAla: 4.957 ± 0.09
0.692GluCys: 0.692 ± 0.029
2.655GluAsp: 2.655 ± 0.06
3.739GluGlu: 3.739 ± 0.069
2.329GluPhe: 2.329 ± 0.053
3.887GluGly: 3.887 ± 0.074
1.187GluHis: 1.187 ± 0.037
4.41GluIle: 4.41 ± 0.081
3.57GluLys: 3.57 ± 0.072
5.251GluLeu: 5.251 ± 0.083
1.831GluMet: 1.831 ± 0.047
2.714GluAsn: 2.714 ± 0.059
1.975GluPro: 1.975 ± 0.052
1.951GluGln: 1.951 ± 0.045
3.523GluArg: 3.523 ± 0.065
3.42GluSer: 3.42 ± 0.06
2.783GluThr: 2.783 ± 0.057
3.851GluVal: 3.851 ± 0.075
0.871GluTrp: 0.871 ± 0.036
2.544GluTyr: 2.544 ± 0.057
0.0GluXaa: 0.0 ± 0.0
Phe
3.132PheAla: 3.132 ± 0.062
0.61PheCys: 0.61 ± 0.025
2.914PheAsp: 2.914 ± 0.067
2.07PheGlu: 2.07 ± 0.05
1.837PhePhe: 1.837 ± 0.051
3.197PheGly: 3.197 ± 0.053
0.814PheHis: 0.814 ± 0.031
2.845PheIle: 2.845 ± 0.061
1.806PheLys: 1.806 ± 0.047
3.275PheLeu: 3.275 ± 0.07
1.186PheMet: 1.186 ± 0.042
2.161PheAsn: 2.161 ± 0.063
1.801PhePro: 1.801 ± 0.047
0.926PheGln: 0.926 ± 0.035
2.259PheArg: 2.259 ± 0.05
3.09PheSer: 3.09 ± 0.062
2.712PheThr: 2.712 ± 0.061
2.539PheVal: 2.539 ± 0.055
0.531PheTrp: 0.531 ± 0.02
1.582PheTyr: 1.582 ± 0.045
0.0PheXaa: 0.0 ± 0.0
Gly
5.664GlyAla: 5.664 ± 0.087
1.112GlyCys: 1.112 ± 0.041
4.091GlyAsp: 4.091 ± 0.08
3.973GlyGlu: 3.973 ± 0.067
3.093GlyPhe: 3.093 ± 0.063
5.121GlyGly: 5.121 ± 0.093
1.608GlyHis: 1.608 ± 0.045
5.475GlyIle: 5.475 ± 0.091
4.201GlyLys: 4.201 ± 0.07
5.651GlyLeu: 5.651 ± 0.081
2.347GlyMet: 2.347 ± 0.052
3.575GlyAsn: 3.575 ± 0.079
1.677GlyPro: 1.677 ± 0.046
1.943GlyGln: 1.943 ± 0.048
4.079GlyArg: 4.079 ± 0.084
4.405GlySer: 4.405 ± 0.078
4.327GlyThr: 4.327 ± 0.083
5.35GlyVal: 5.35 ± 0.083
1.094GlyTrp: 1.094 ± 0.035
3.322GlyTyr: 3.322 ± 0.072
0.0GlyXaa: 0.0 ± 0.0
His
1.377HisAla: 1.377 ± 0.04
0.288HisCys: 0.288 ± 0.017
1.282HisAsp: 1.282 ± 0.04
0.951HisGlu: 0.951 ± 0.035
0.907HisPhe: 0.907 ± 0.031
1.497HisGly: 1.497 ± 0.045
0.536HisHis: 0.536 ± 0.034
1.498HisIle: 1.498 ± 0.051
0.93HisLys: 0.93 ± 0.032
1.662HisLeu: 1.662 ± 0.044
0.503HisMet: 0.503 ± 0.025
0.996HisAsn: 0.996 ± 0.033
1.144HisPro: 1.144 ± 0.03
0.512HisGln: 0.512 ± 0.023
1.15HisArg: 1.15 ± 0.04
1.248HisSer: 1.248 ± 0.04
1.203HisThr: 1.203 ± 0.041
1.149HisVal: 1.149 ± 0.036
0.257HisTrp: 0.257 ± 0.017
0.843HisTyr: 0.843 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
6.162IleAla: 6.162 ± 0.098
0.919IleCys: 0.919 ± 0.033
5.152IleAsp: 5.152 ± 0.083
4.284IleGlu: 4.284 ± 0.072
2.685IlePhe: 2.685 ± 0.064
4.792IleGly: 4.792 ± 0.073
1.26IleHis: 1.26 ± 0.033
4.596IleIle: 4.596 ± 0.091
3.33IleLys: 3.33 ± 0.07
5.23IleLeu: 5.23 ± 0.084
1.66IleMet: 1.66 ± 0.042
3.257IleAsn: 3.257 ± 0.068
3.233IlePro: 3.233 ± 0.066
1.652IleGln: 1.652 ± 0.045
3.289IleArg: 3.289 ± 0.061
4.785IleSer: 4.785 ± 0.069
4.069IleThr: 4.069 ± 0.073
4.79IleVal: 4.79 ± 0.087
0.706IleTrp: 0.706 ± 0.028
2.455IleTyr: 2.455 ± 0.056
0.0IleXaa: 0.0 ± 0.0
Lys
4.471LysAla: 4.471 ± 0.083
0.529LysCys: 0.529 ± 0.025
2.764LysAsp: 2.764 ± 0.061
3.66LysGlu: 3.66 ± 0.079
1.803LysPhe: 1.803 ± 0.044
3.643LysGly: 3.643 ± 0.067
0.946LysHis: 0.946 ± 0.034
3.373LysIle: 3.373 ± 0.06
3.194LysLys: 3.194 ± 0.067
3.799LysLeu: 3.799 ± 0.069
1.607LysMet: 1.607 ± 0.042
2.286LysAsn: 2.286 ± 0.053
1.949LysPro: 1.949 ± 0.05
1.432LysGln: 1.432 ± 0.045
2.657LysArg: 2.657 ± 0.05
3.076LysSer: 3.076 ± 0.057
2.629LysThr: 2.629 ± 0.055
3.48LysVal: 3.48 ± 0.064
0.711LysTrp: 0.711 ± 0.028
2.188LysTyr: 2.188 ± 0.047
0.0LysXaa: 0.0 ± 0.0
Leu
6.947LeuAla: 6.947 ± 0.102
1.177LeuCys: 1.177 ± 0.035
5.282LeuAsp: 5.282 ± 0.097
4.378LeuGlu: 4.378 ± 0.073
3.519LeuPhe: 3.519 ± 0.06
5.789LeuGly: 5.789 ± 0.09
1.724LeuHis: 1.724 ± 0.041
5.022LeuIle: 5.022 ± 0.093
4.326LeuLys: 4.326 ± 0.077
7.407LeuLeu: 7.407 ± 0.121
2.366LeuMet: 2.366 ± 0.053
3.926LeuAsn: 3.926 ± 0.062
4.225LeuPro: 4.225 ± 0.071
2.484LeuGln: 2.484 ± 0.055
5.417LeuArg: 5.417 ± 0.082
6.517LeuSer: 6.517 ± 0.095
5.491LeuThr: 5.491 ± 0.085
5.012LeuVal: 5.012 ± 0.079
1.03LeuTrp: 1.03 ± 0.037
3.253LeuTyr: 3.253 ± 0.059
0.0LeuXaa: 0.0 ± 0.0
Met
2.71MetAla: 2.71 ± 0.056
0.296MetCys: 0.296 ± 0.018
1.305MetAsp: 1.305 ± 0.04
1.703MetGlu: 1.703 ± 0.046
1.055MetPhe: 1.055 ± 0.036
1.847MetGly: 1.847 ± 0.05
0.598MetHis: 0.598 ± 0.025
1.678MetIle: 1.678 ± 0.045
1.924MetLys: 1.924 ± 0.048
2.997MetLeu: 2.997 ± 0.066
0.923MetMet: 0.923 ± 0.033
1.269MetAsn: 1.269 ± 0.039
1.475MetPro: 1.475 ± 0.039
0.942MetGln: 0.942 ± 0.033
1.881MetArg: 1.881 ± 0.041
2.027MetSer: 2.027 ± 0.054
1.77MetThr: 1.77 ± 0.04
1.68MetVal: 1.68 ± 0.04
0.349MetTrp: 0.349 ± 0.02
0.85MetTyr: 0.85 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
3.676AsnAla: 3.676 ± 0.069
0.516AsnCys: 0.516 ± 0.03
2.591AsnAsp: 2.591 ± 0.06
2.308AsnGlu: 2.308 ± 0.053
1.843AsnPhe: 1.843 ± 0.049
3.635AsnGly: 3.635 ± 0.073
0.895AsnHis: 0.895 ± 0.029
3.389AsnIle: 3.389 ± 0.073
2.101AsnLys: 2.101 ± 0.05
3.822AsnLeu: 3.822 ± 0.07
1.18AsnMet: 1.18 ± 0.036
2.238AsnAsn: 2.238 ± 0.062
2.862AsnPro: 2.862 ± 0.054
1.192AsnGln: 1.192 ± 0.041
2.624AsnArg: 2.624 ± 0.057
2.739AsnSer: 2.739 ± 0.06
2.575AsnThr: 2.575 ± 0.056
3.223AsnVal: 3.223 ± 0.067
0.546AsnTrp: 0.546 ± 0.026
1.851AsnTyr: 1.851 ± 0.056
0.0AsnXaa: 0.0 ± 0.0
Pro
3.907ProAla: 3.907 ± 0.068
0.405ProCys: 0.405 ± 0.022
3.511ProAsp: 3.511 ± 0.055
3.419ProGlu: 3.419 ± 0.069
1.646ProPhe: 1.646 ± 0.043
3.167ProGly: 3.167 ± 0.057
0.814ProHis: 0.814 ± 0.035
2.142ProIle: 2.142 ± 0.049
1.807ProLys: 1.807 ± 0.051
3.368ProLeu: 3.368 ± 0.062
1.147ProMet: 1.147 ± 0.037
1.417ProAsn: 1.417 ± 0.041
1.041ProPro: 1.041 ± 0.042
1.436ProGln: 1.436 ± 0.043
1.986ProArg: 1.986 ± 0.045
2.897ProSer: 2.897 ± 0.054
2.265ProThr: 2.265 ± 0.05
3.576ProVal: 3.576 ± 0.067
0.526ProTrp: 0.526 ± 0.024
1.756ProTyr: 1.756 ± 0.043
0.0ProXaa: 0.0 ± 0.0
Gln
2.254GlnAla: 2.254 ± 0.058
0.318GlnCys: 0.318 ± 0.021
1.167GlnAsp: 1.167 ± 0.04
1.637GlnGlu: 1.637 ± 0.045
1.181GlnPhe: 1.181 ± 0.032
1.777GlnGly: 1.777 ± 0.038
0.534GlnHis: 0.534 ± 0.029
1.942GlnIle: 1.942 ± 0.046
1.548GlnLys: 1.548 ± 0.045
2.664GlnLeu: 2.664 ± 0.055
0.938GlnMet: 0.938 ± 0.038
1.235GlnAsn: 1.235 ± 0.043
1.229GlnPro: 1.229 ± 0.044
1.254GlnGln: 1.254 ± 0.045
1.702GlnArg: 1.702 ± 0.045
1.845GlnSer: 1.845 ± 0.045
1.507GlnThr: 1.507 ± 0.042
1.584GlnVal: 1.584 ± 0.042
0.517GlnTrp: 0.517 ± 0.023
1.172GlnTyr: 1.172 ± 0.038
0.0GlnXaa: 0.0 ± 0.0
Arg
3.772ArgAla: 3.772 ± 0.071
0.638ArgCys: 0.638 ± 0.024
3.15ArgAsp: 3.15 ± 0.067
3.377ArgGlu: 3.377 ± 0.075
2.534ArgPhe: 2.534 ± 0.053
3.229ArgGly: 3.229 ± 0.061
1.521ArgHis: 1.521 ± 0.042
4.079ArgIle: 4.079 ± 0.062
2.992ArgLys: 2.992 ± 0.06
5.561ArgLeu: 5.561 ± 0.079
1.884ArgMet: 1.884 ± 0.041
2.756ArgAsn: 2.756 ± 0.06
2.224ArgPro: 2.224 ± 0.054
2.082ArgGln: 2.082 ± 0.05
3.956ArgArg: 3.956 ± 0.088
2.904ArgSer: 2.904 ± 0.056
2.794ArgThr: 2.794 ± 0.057
3.369ArgVal: 3.369 ± 0.065
0.856ArgTrp: 0.856 ± 0.032
2.564ArgTyr: 2.564 ± 0.063
0.0ArgXaa: 0.0 ± 0.0
Ser
5.442SerAla: 5.442 ± 0.094
0.776SerCys: 0.776 ± 0.027
4.209SerAsp: 4.209 ± 0.064
3.48SerGlu: 3.48 ± 0.055
2.756SerPhe: 2.756 ± 0.061
5.219SerGly: 5.219 ± 0.084
1.374SerHis: 1.374 ± 0.039
4.308SerIle: 4.308 ± 0.067
2.808SerLys: 2.808 ± 0.058
6.154SerLeu: 6.154 ± 0.092
1.807SerMet: 1.807 ± 0.045
2.53SerAsn: 2.53 ± 0.058
2.88SerPro: 2.88 ± 0.063
1.862SerGln: 1.862 ± 0.045
3.808SerArg: 3.808 ± 0.068
4.32SerSer: 4.32 ± 0.078
3.78SerThr: 3.78 ± 0.07
4.741SerVal: 4.741 ± 0.074
0.842SerTrp: 0.842 ± 0.032
2.542SerTyr: 2.542 ± 0.061
0.0SerXaa: 0.0 ± 0.0
Thr
4.838ThrAla: 4.838 ± 0.084
0.602ThrCys: 0.602 ± 0.025
3.989ThrAsp: 3.989 ± 0.065
3.055ThrGlu: 3.055 ± 0.059
2.607ThrPhe: 2.607 ± 0.058
4.501ThrGly: 4.501 ± 0.073
1.116ThrHis: 1.116 ± 0.039
3.975ThrIle: 3.975 ± 0.072
2.127ThrLys: 2.127 ± 0.048
5.646ThrLeu: 5.646 ± 0.088
1.306ThrMet: 1.306 ± 0.037
2.081ThrAsn: 2.081 ± 0.056
3.417ThrPro: 3.417 ± 0.06
1.5ThrGln: 1.5 ± 0.044
2.599ThrArg: 2.599 ± 0.05
3.779ThrSer: 3.779 ± 0.068
3.459ThrThr: 3.459 ± 0.075
4.684ThrVal: 4.684 ± 0.083
0.714ThrTrp: 0.714 ± 0.026
2.259ThrTyr: 2.259 ± 0.057
0.0ThrXaa: 0.0 ± 0.0
Val
6.288ValAla: 6.288 ± 0.1
0.96ValCys: 0.96 ± 0.034
4.392ValAsp: 4.392 ± 0.075
4.064ValGlu: 4.064 ± 0.069
2.538ValPhe: 2.538 ± 0.052
4.419ValGly: 4.419 ± 0.074
1.066ValHis: 1.066 ± 0.034
4.481ValIle: 4.481 ± 0.076
3.708ValLys: 3.708 ± 0.064
5.393ValLeu: 5.393 ± 0.085
1.972ValMet: 1.972 ± 0.048
3.355ValAsn: 3.355 ± 0.062
2.88ValPro: 2.88 ± 0.057
1.438ValGln: 1.438 ± 0.032
3.578ValArg: 3.578 ± 0.067
4.8ValSer: 4.8 ± 0.071
4.298ValThr: 4.298 ± 0.078
4.986ValVal: 4.986 ± 0.075
0.794ValTrp: 0.794 ± 0.029
2.552ValTyr: 2.552 ± 0.052
0.0ValXaa: 0.0 ± 0.0
Trp
0.894TrpAla: 0.894 ± 0.028
0.181TrpCys: 0.181 ± 0.014
0.714TrpAsp: 0.714 ± 0.027
0.761TrpGlu: 0.761 ± 0.028
0.5TrpPhe: 0.5 ± 0.027
0.989TrpGly: 0.989 ± 0.037
0.36TrpHis: 0.36 ± 0.021
0.885TrpIle: 0.885 ± 0.039
0.617TrpLys: 0.617 ± 0.023
1.246TrpLeu: 1.246 ± 0.039
0.433TrpMet: 0.433 ± 0.024
0.738TrpAsn: 0.738 ± 0.028
0.38TrpPro: 0.38 ± 0.019
0.493TrpGln: 0.493 ± 0.023
0.769TrpArg: 0.769 ± 0.031
0.844TrpSer: 0.844 ± 0.032
0.758TrpThr: 0.758 ± 0.028
0.794TrpVal: 0.794 ± 0.033
0.257TrpTrp: 0.257 ± 0.019
0.471TrpTyr: 0.471 ± 0.025
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.084TyrAla: 3.084 ± 0.054
0.573TyrCys: 0.573 ± 0.025
2.645TyrAsp: 2.645 ± 0.052
2.053TyrGlu: 2.053 ± 0.047
1.815TyrPhe: 1.815 ± 0.04
3.013TyrGly: 3.013 ± 0.059
0.857TyrHis: 0.857 ± 0.031
2.678TyrIle: 2.678 ± 0.06
1.792TyrLys: 1.792 ± 0.043
3.349TyrLeu: 3.349 ± 0.06
1.013TyrMet: 1.013 ± 0.037
2.207TyrAsn: 2.207 ± 0.063
1.83TyrPro: 1.83 ± 0.046
1.01TyrGln: 1.01 ± 0.034
2.439TyrArg: 2.439 ± 0.059
2.678TyrSer: 2.678 ± 0.056
2.533TyrThr: 2.533 ± 0.055
2.495TyrVal: 2.495 ± 0.055
0.516TyrTrp: 0.516 ± 0.024
1.84TyrTyr: 1.84 ± 0.05
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2553 proteins (928564 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski