Amino acid dipepetide frequency for Vogesella sp. EB

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.989AlaAla: 17.989 ± 0.213	1.29AlaCys: 1.29 ± 0.042	6.613AlaAsp: 6.613 ± 0.089	7.016AlaGlu: 7.016 ± 0.096	3.873AlaPhe: 3.873 ± 0.071	10.135AlaGly: 10.135 ± 0.117	2.318AlaHis: 2.318 ± 0.057	5.786AlaIle: 5.786 ± 0.085	3.718AlaLys: 3.718 ± 0.078	14.916AlaLeu: 14.916 ± 0.157	3.422AlaMet: 3.422 ± 0.065	3.415AlaAsn: 3.415 ± 0.063	4.985AlaPro: 4.985 ± 0.102	5.345AlaGln: 5.345 ± 0.089	8.113AlaArg: 8.113 ± 0.116	6.461AlaSer: 6.461 ± 0.096	5.56AlaThr: 5.56 ± 0.081	8.27AlaVal: 8.27 ± 0.104	1.605AlaTrp: 1.605 ± 0.049	2.408AlaTyr: 2.408 ± 0.049	0.0AlaXaa: 0.0 ± 0.0
Cys	1.05CysAla: 1.05 ± 0.034	0.157CysCys: 0.157 ± 0.014	0.577CysAsp: 0.577 ± 0.024	0.485CysGlu: 0.485 ± 0.023	0.316CysPhe: 0.316 ± 0.018	1.074CysGly: 1.074 ± 0.033	0.371CysHis: 0.371 ± 0.018	0.397CysIle: 0.397 ± 0.02	0.274CysLys: 0.274 ± 0.017	0.971CysLeu: 0.971 ± 0.034	0.188CysMet: 0.188 ± 0.014	0.279CysAsn: 0.279 ± 0.017	0.52CysPro: 0.52 ± 0.025	0.407CysGln: 0.407 ± 0.02	0.698CysArg: 0.698 ± 0.029	0.542CysSer: 0.542 ± 0.022	0.448CysThr: 0.448 ± 0.021	0.681CysVal: 0.681 ± 0.029	0.139CysTrp: 0.139 ± 0.011	0.298CysTyr: 0.298 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	6.713AspAla: 6.713 ± 0.087	0.564AspCys: 0.564 ± 0.023	3.13AspAsp: 3.13 ± 0.061	3.318AspGlu: 3.318 ± 0.068	2.169AspPhe: 2.169 ± 0.047	4.974AspGly: 4.974 ± 0.085	1.033AspHis: 1.033 ± 0.03	3.119AspIle: 3.119 ± 0.061	2.259AspLys: 2.259 ± 0.062	4.731AspLeu: 4.731 ± 0.068	1.376AspMet: 1.376 ± 0.038	1.636AspAsn: 1.636 ± 0.042	2.33AspPro: 2.33 ± 0.049	1.471AspGln: 1.471 ± 0.04	2.668AspArg: 2.668 ± 0.062	3.125AspSer: 3.125 ± 0.06	2.636AspThr: 2.636 ± 0.057	3.809AspVal: 3.809 ± 0.074	1.003AspTrp: 1.003 ± 0.039	1.709AspTyr: 1.709 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	6.474GluAla: 6.474 ± 0.091	0.433GluCys: 0.433 ± 0.023	2.206GluAsp: 2.206 ± 0.046	2.555GluGlu: 2.555 ± 0.062	1.765GluPhe: 1.765 ± 0.046	3.284GluGly: 3.284 ± 0.062	1.397GluHis: 1.397 ± 0.04	2.77GluIle: 2.77 ± 0.067	1.972GluLys: 1.972 ± 0.05	6.666GluLeu: 6.666 ± 0.094	1.354GluMet: 1.354 ± 0.043	1.338GluAsn: 1.338 ± 0.043	1.982GluPro: 1.982 ± 0.054	3.27GluGln: 3.27 ± 0.064	4.137GluArg: 4.137 ± 0.071	2.351GluSer: 2.351 ± 0.055	2.404GluThr: 2.404 ± 0.048	4.293GluVal: 4.293 ± 0.077	0.69GluTrp: 0.69 ± 0.03	1.146GluTyr: 1.146 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	4.416PheAla: 4.416 ± 0.064	0.448PheCys: 0.448 ± 0.021	2.529PheAsp: 2.529 ± 0.051	1.884PheGlu: 1.884 ± 0.052	1.351PhePhe: 1.351 ± 0.043	3.253PheGly: 3.253 ± 0.065	0.788PheHis: 0.788 ± 0.029	1.765PheIle: 1.765 ± 0.049	1.165PheLys: 1.165 ± 0.036	3.125PheLeu: 3.125 ± 0.057	0.857PheMet: 0.857 ± 0.034	1.238PheAsn: 1.238 ± 0.037	1.414PhePro: 1.414 ± 0.032	1.08PheGln: 1.08 ± 0.029	1.91PheArg: 1.91 ± 0.044	2.315PheSer: 2.315 ± 0.054	1.7PheThr: 1.7 ± 0.047	2.725PheVal: 2.725 ± 0.048	0.524PheTrp: 0.524 ± 0.025	1.009PheTyr: 1.009 ± 0.032	0.0PheXaa: 0.0 ± 0.0
Gly	7.6GlyAla: 7.6 ± 0.108	0.95GlyCys: 0.95 ± 0.035	4.221GlyAsp: 4.221 ± 0.071	4.745GlyGlu: 4.745 ± 0.075	3.205GlyPhe: 3.205 ± 0.066	6.382GlyGly: 6.382 ± 0.099	2.005GlyHis: 2.005 ± 0.045	4.324GlyIle: 4.324 ± 0.073	3.786GlyLys: 3.786 ± 0.071	8.344GlyLeu: 8.344 ± 0.102	2.309GlyMet: 2.309 ± 0.052	2.372GlyAsn: 2.372 ± 0.053	2.085GlyPro: 2.085 ± 0.056	3.405GlyGln: 3.405 ± 0.062	4.988GlyArg: 4.988 ± 0.075	4.652GlySer: 4.652 ± 0.082	3.441GlyThr: 3.441 ± 0.066	6.237GlyVal: 6.237 ± 0.079	1.348GlyTrp: 1.348 ± 0.042	2.416GlyTyr: 2.416 ± 0.06	0.0GlyXaa: 0.0 ± 0.0
His	2.616HisAla: 2.616 ± 0.062	0.341HisCys: 0.341 ± 0.018	1.416HisAsp: 1.416 ± 0.042	1.149HisGlu: 1.149 ± 0.037	1.001HisPhe: 1.001 ± 0.034	2.227HisGly: 2.227 ± 0.057	0.718HisHis: 0.718 ± 0.028	1.138HisIle: 1.138 ± 0.036	0.653HisLys: 0.653 ± 0.028	2.266HisLeu: 2.266 ± 0.056	0.462HisMet: 0.462 ± 0.022	0.643HisAsn: 0.643 ± 0.029	1.469HisPro: 1.469 ± 0.038	0.932HisGln: 0.932 ± 0.031	1.293HisArg: 1.293 ± 0.04	1.233HisSer: 1.233 ± 0.041	1.059HisThr: 1.059 ± 0.033	1.451HisVal: 1.451 ± 0.041	0.394HisTrp: 0.394 ± 0.019	0.815HisTyr: 0.815 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	6.449IleAla: 6.449 ± 0.09	0.452IleCys: 0.452 ± 0.022	3.275IleAsp: 3.275 ± 0.058	3.031IleGlu: 3.031 ± 0.066	1.305IlePhe: 1.305 ± 0.043	4.433IleGly: 4.433 ± 0.067	1.016IleHis: 1.016 ± 0.032	2.049IleIle: 2.049 ± 0.054	1.843IleLys: 1.843 ± 0.049	3.868IleLeu: 3.868 ± 0.069	0.906IleMet: 0.906 ± 0.03	1.729IleAsn: 1.729 ± 0.045	2.088IlePro: 2.088 ± 0.045	1.414IleGln: 1.414 ± 0.042	3.02IleArg: 3.02 ± 0.06	2.834IleSer: 2.834 ± 0.056	2.592IleThr: 2.592 ± 0.064	3.62IleVal: 3.62 ± 0.067	0.456IleTrp: 0.456 ± 0.02	1.067IleTyr: 1.067 ± 0.033	0.0IleXaa: 0.0 ± 0.0
Lys	3.861LysAla: 3.861 ± 0.09	0.169LysCys: 0.169 ± 0.013	1.696LysAsp: 1.696 ± 0.049	1.684LysGlu: 1.684 ± 0.048	0.866LysPhe: 0.866 ± 0.031	2.425LysGly: 2.425 ± 0.068	0.782LysHis: 0.782 ± 0.028	1.692LysIle: 1.692 ± 0.046	1.525LysLys: 1.525 ± 0.045	4.159LysLeu: 4.159 ± 0.075	0.87LysMet: 0.87 ± 0.034	1.044LysAsn: 1.044 ± 0.042	2.036LysPro: 2.036 ± 0.048	1.782LysGln: 1.782 ± 0.048	2.216LysArg: 2.216 ± 0.052	1.899LysSer: 1.899 ± 0.048	1.909LysThr: 1.909 ± 0.046	2.811LysVal: 2.811 ± 0.064	0.352LysTrp: 0.352 ± 0.019	0.771LysTyr: 0.771 ± 0.032	0.0LysXaa: 0.0 ± 0.0
Leu	17.155LeuAla: 17.155 ± 0.201	1.152LeuCys: 1.152 ± 0.034	6.37LeuAsp: 6.37 ± 0.087	5.215LeuGlu: 5.215 ± 0.085	4.034LeuPhe: 4.034 ± 0.076	8.408LeuGly: 8.408 ± 0.103	2.464LeuHis: 2.464 ± 0.055	4.93LeuIle: 4.93 ± 0.081	3.982LeuLys: 3.982 ± 0.068	15.031LeuLeu: 15.031 ± 0.251	2.634LeuMet: 2.634 ± 0.05	3.103LeuAsn: 3.103 ± 0.06	6.922LeuPro: 6.922 ± 0.105	5.796LeuGln: 5.796 ± 0.111	7.126LeuArg: 7.126 ± 0.105	6.919LeuSer: 6.919 ± 0.101	5.707LeuThr: 5.707 ± 0.083	7.358LeuVal: 7.358 ± 0.093	1.401LeuTrp: 1.401 ± 0.05	2.471LeuTyr: 2.471 ± 0.052	0.0LeuXaa: 0.0 ± 0.0
Met	3.031MetAla: 3.031 ± 0.053	0.165MetCys: 0.165 ± 0.013	1.098MetAsp: 1.098 ± 0.036	1.029MetGlu: 1.029 ± 0.034	0.716MetPhe: 0.716 ± 0.027	1.734MetGly: 1.734 ± 0.044	0.507MetHis: 0.507 ± 0.024	1.071MetIle: 1.071 ± 0.032	1.07MetLys: 1.07 ± 0.034	2.947MetLeu: 2.947 ± 0.065	0.653MetMet: 0.653 ± 0.028	0.853MetAsn: 0.853 ± 0.032	1.241MetPro: 1.241 ± 0.039	1.246MetGln: 1.246 ± 0.04	1.415MetArg: 1.415 ± 0.042	1.724MetSer: 1.724 ± 0.043	1.461MetThr: 1.461 ± 0.042	1.76MetVal: 1.76 ± 0.045	0.216MetTrp: 0.216 ± 0.014	0.401MetTyr: 0.401 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	3.125AsnAla: 3.125 ± 0.057	0.255AsnCys: 0.255 ± 0.016	1.468AsnAsp: 1.468 ± 0.04	1.358AsnGlu: 1.358 ± 0.041	0.921AsnPhe: 0.921 ± 0.033	2.419AsnGly: 2.419 ± 0.055	0.637AsnHis: 0.637 ± 0.024	1.475AsnIle: 1.475 ± 0.038	1.019AsnLys: 1.019 ± 0.037	3.085AsnLeu: 3.085 ± 0.054	0.682AsnMet: 0.682 ± 0.028	0.88AsnAsn: 0.88 ± 0.037	1.884AsnPro: 1.884 ± 0.043	1.059AsnGln: 1.059 ± 0.036	1.858AsnArg: 1.858 ± 0.044	1.408AsnSer: 1.408 ± 0.044	1.422AsnThr: 1.422 ± 0.038	2.11AsnVal: 2.11 ± 0.051	0.438AsnTrp: 0.438 ± 0.02	0.74AsnTyr: 0.74 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	6.534ProAla: 6.534 ± 0.105	0.386ProCys: 0.386 ± 0.02	2.895ProAsp: 2.895 ± 0.058	2.918ProGlu: 2.918 ± 0.057	1.779ProPhe: 1.779 ± 0.042	3.58ProGly: 3.58 ± 0.071	1.076ProHis: 1.076 ± 0.037	1.579ProIle: 1.579 ± 0.044	1.357ProLys: 1.357 ± 0.04	5.952ProLeu: 5.952 ± 0.1	1.052ProMet: 1.052 ± 0.031	1.111ProAsn: 1.111 ± 0.036	2.248ProPro: 2.248 ± 0.06	2.656ProGln: 2.656 ± 0.059	2.404ProArg: 2.404 ± 0.058	2.195ProSer: 2.195 ± 0.048	1.942ProThr: 1.942 ± 0.041	3.879ProVal: 3.879 ± 0.075	0.675ProTrp: 0.675 ± 0.03	1.233ProTyr: 1.233 ± 0.04	0.0ProXaa: 0.0 ± 0.0
Gln	5.758GlnAla: 5.758 ± 0.097	0.318GlnCys: 0.318 ± 0.019	1.899GlnAsp: 1.899 ± 0.052	1.815GlnGlu: 1.815 ± 0.049	1.468GlnPhe: 1.468 ± 0.039	3.171GlnGly: 3.171 ± 0.064	1.439GlnHis: 1.439 ± 0.043	1.974GlnIle: 1.974 ± 0.043	1.294GlnLys: 1.294 ± 0.043	6.485GlnLeu: 6.485 ± 0.11	1.028GlnMet: 1.028 ± 0.034	0.97GlnAsn: 0.97 ± 0.034	2.617GlnPro: 2.617 ± 0.056	3.851GlnGln: 3.851 ± 0.087	3.813GlnArg: 3.813 ± 0.078	2.093GlnSer: 2.093 ± 0.049	1.886GlnThr: 1.886 ± 0.048	3.048GlnVal: 3.048 ± 0.052	0.751GlnTrp: 0.751 ± 0.031	0.97GlnTyr: 0.97 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	6.268ArgAla: 6.268 ± 0.087	0.572ArgCys: 0.572 ± 0.024	3.738ArgAsp: 3.738 ± 0.071	3.991ArgGlu: 3.991 ± 0.077	2.84ArgPhe: 2.84 ± 0.054	4.028ArgGly: 4.028 ± 0.069	1.982ArgHis: 1.982 ± 0.055	3.431ArgIle: 3.431 ± 0.065	2.074ArgLys: 2.074 ± 0.049	8.155ArgLeu: 8.155 ± 0.117	1.57ArgMet: 1.57 ± 0.041	1.741ArgAsn: 1.741 ± 0.046	2.691ArgPro: 2.691 ± 0.05	3.728ArgGln: 3.728 ± 0.07	4.323ArgArg: 4.323 ± 0.078	3.018ArgSer: 3.018 ± 0.052	2.387ArgThr: 2.387 ± 0.05	4.469ArgVal: 4.469 ± 0.072	0.993ArgTrp: 0.993 ± 0.037	2.071ArgTyr: 2.071 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	5.943SerAla: 5.943 ± 0.087	0.583SerCys: 0.583 ± 0.027	2.731SerAsp: 2.731 ± 0.058	2.771SerGlu: 2.771 ± 0.052	2.172SerPhe: 2.172 ± 0.048	5.334SerGly: 5.334 ± 0.087	1.357SerHis: 1.357 ± 0.036	2.495SerIle: 2.495 ± 0.058	1.783SerLys: 1.783 ± 0.046	6.364SerLeu: 6.364 ± 0.09	1.291SerMet: 1.291 ± 0.037	1.6SerAsn: 1.6 ± 0.048	2.546SerPro: 2.546 ± 0.049	2.227SerGln: 2.227 ± 0.052	3.565SerArg: 3.565 ± 0.064	3.188SerSer: 3.188 ± 0.076	2.511SerThr: 2.511 ± 0.057	3.846SerVal: 3.846 ± 0.063	0.804SerTrp: 0.804 ± 0.028	1.499SerTyr: 1.499 ± 0.047	0.0SerXaa: 0.0 ± 0.0
Thr	5.446ThrAla: 5.446 ± 0.08	0.391ThrCys: 0.391 ± 0.02	2.171ThrAsp: 2.171 ± 0.042	2.14ThrGlu: 2.14 ± 0.056	1.601ThrPhe: 1.601 ± 0.037	3.934ThrGly: 3.934 ± 0.076	0.936ThrHis: 0.936 ± 0.032	2.203ThrIle: 2.203 ± 0.049	1.081ThrLys: 1.081 ± 0.041	7.086ThrLeu: 7.086 ± 0.099	0.972ThrMet: 0.972 ± 0.031	1.054ThrAsn: 1.054 ± 0.037	3.18ThrPro: 3.18 ± 0.062	1.712ThrGln: 1.712 ± 0.039	3.085ThrArg: 3.085 ± 0.059	2.243ThrSer: 2.243 ± 0.049	2.328ThrThr: 2.328 ± 0.055	3.896ThrVal: 3.896 ± 0.067	0.452ThrTrp: 0.452 ± 0.021	0.949ThrTyr: 0.949 ± 0.031	0.0ThrXaa: 0.0 ± 0.0
Val	9.242ValAla: 9.242 ± 0.116	0.802ValCys: 0.802 ± 0.029	3.884ValAsp: 3.884 ± 0.068	3.83ValGlu: 3.83 ± 0.064	2.586ValPhe: 2.586 ± 0.052	5.058ValGly: 5.058 ± 0.081	1.377ValHis: 1.377 ± 0.036	3.835ValIle: 3.835 ± 0.07	2.617ValLys: 2.617 ± 0.067	8.45ValLeu: 8.45 ± 0.105	1.939ValMet: 1.939 ± 0.048	2.19ValAsn: 2.19 ± 0.051	3.198ValPro: 3.198 ± 0.062	2.604ValGln: 2.604 ± 0.049	4.358ValArg: 4.358 ± 0.076	4.467ValSer: 4.467 ± 0.074	3.801ValThr: 3.801 ± 0.065	5.685ValVal: 5.685 ± 0.098	0.858ValTrp: 0.858 ± 0.034	1.586ValTyr: 1.586 ± 0.038	0.0ValXaa: 0.0 ± 0.0
Trp	1.01TrpAla: 1.01 ± 0.037	0.175TrpCys: 0.175 ± 0.014	0.536TrpAsp: 0.536 ± 0.024	0.469TrpGlu: 0.469 ± 0.02	0.515TrpPhe: 0.515 ± 0.024	0.873TrpGly: 0.873 ± 0.029	0.42TrpHis: 0.42 ± 0.023	0.489TrpIle: 0.489 ± 0.021	0.381TrpLys: 0.381 ± 0.019	2.588TrpLeu: 2.588 ± 0.071	0.332TrpMet: 0.332 ± 0.021	0.341TrpAsn: 0.341 ± 0.019	0.722TrpPro: 0.722 ± 0.033	1.305TrpGln: 1.305 ± 0.046	1.094TrpArg: 1.094 ± 0.037	0.634TrpSer: 0.634 ± 0.028	0.482TrpThr: 0.482 ± 0.026	0.827TrpVal: 0.827 ± 0.031	0.258TrpTrp: 0.258 ± 0.019	0.286TrpTyr: 0.286 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.559TyrAla: 2.559 ± 0.055	0.268TyrCys: 0.268 ± 0.017	1.318TyrAsp: 1.318 ± 0.034	1.083TyrGlu: 1.083 ± 0.033	1.014TyrPhe: 1.014 ± 0.036	2.092TyrGly: 2.092 ± 0.049	0.653TyrHis: 0.653 ± 0.025	0.925TyrIle: 0.925 ± 0.028	0.696TyrLys: 0.696 ± 0.03	2.985TyrLeu: 2.985 ± 0.055	0.443TyrMet: 0.443 ± 0.025	0.704TyrAsn: 0.704 ± 0.03	1.294TyrPro: 1.294 ± 0.036	1.357TyrGln: 1.357 ± 0.039	1.986TyrArg: 1.986 ± 0.048	1.308TyrSer: 1.308 ± 0.037	1.189TyrThr: 1.189 ± 0.038	1.591TyrVal: 1.591 ± 0.04	0.378TyrTrp: 0.378 ± 0.018	0.71TyrTyr: 0.71 ± 0.033	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3143 proteins (978495 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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