Amino acid dipepetide frequency for Bacillus sp. UMB0899

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.684AlaAla: 4.684 ± 0.08
0.545AlaCys: 0.545 ± 0.019
2.904AlaAsp: 2.904 ± 0.043
4.243AlaGlu: 4.243 ± 0.062
3.001AlaPhe: 3.001 ± 0.051
4.549AlaGly: 4.549 ± 0.066
1.154AlaHis: 1.154 ± 0.029
5.618AlaIle: 5.618 ± 0.068
4.408AlaLys: 4.408 ± 0.064
6.453AlaLeu: 6.453 ± 0.08
1.772AlaMet: 1.772 ± 0.038
2.738AlaAsn: 2.738 ± 0.044
1.807AlaPro: 1.807 ± 0.041
1.939AlaGln: 1.939 ± 0.043
2.141AlaArg: 2.141 ± 0.039
3.769AlaSer: 3.769 ± 0.053
3.256AlaThr: 3.256 ± 0.055
4.733AlaVal: 4.733 ± 0.053
0.585AlaTrp: 0.585 ± 0.022
2.113AlaTyr: 2.113 ± 0.043
0.0AlaXaa: 0.0 ± 0.0
Cys
0.406CysAla: 0.406 ± 0.017
0.105CysCys: 0.105 ± 0.009
0.386CysAsp: 0.386 ± 0.018
0.51CysGlu: 0.51 ± 0.019
0.363CysPhe: 0.363 ± 0.015
0.631CysGly: 0.631 ± 0.021
0.209CysHis: 0.209 ± 0.012
0.6CysIle: 0.6 ± 0.021
0.408CysLys: 0.408 ± 0.018
0.715CysLeu: 0.715 ± 0.025
0.185CysMet: 0.185 ± 0.012
0.307CysAsn: 0.307 ± 0.015
0.334CysPro: 0.334 ± 0.018
0.262CysGln: 0.262 ± 0.013
0.267CysArg: 0.267 ± 0.014
0.526CysSer: 0.526 ± 0.019
0.384CysThr: 0.384 ± 0.017
0.425CysVal: 0.425 ± 0.018
0.081CysTrp: 0.081 ± 0.007
0.278CysTyr: 0.278 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
2.829AspAla: 2.829 ± 0.047
0.372AspCys: 0.372 ± 0.016
2.408AspAsp: 2.408 ± 0.045
4.429AspGlu: 4.429 ± 0.059
2.41AspPhe: 2.41 ± 0.039
3.172AspGly: 3.172 ± 0.051
1.312AspHis: 1.312 ± 0.031
4.321AspIle: 4.321 ± 0.059
3.221AspLys: 3.221 ± 0.044
5.134AspLeu: 5.134 ± 0.061
1.334AspMet: 1.334 ± 0.031
1.879AspAsn: 1.879 ± 0.038
1.973AspPro: 1.973 ± 0.031
2.291AspGln: 2.291 ± 0.037
2.093AspArg: 2.093 ± 0.038
2.76AspSer: 2.76 ± 0.048
2.496AspThr: 2.496 ± 0.038
3.764AspVal: 3.764 ± 0.054
0.663AspTrp: 0.663 ± 0.02
2.226AspTyr: 2.226 ± 0.036
0.0AspXaa: 0.0 ± 0.0
Glu
4.901GluAla: 4.901 ± 0.071
0.429GluCys: 0.429 ± 0.016
4.002GluAsp: 4.002 ± 0.063
7.184GluGlu: 7.184 ± 0.1
2.817GluPhe: 2.817 ± 0.039
4.248GluGly: 4.248 ± 0.052
1.585GluHis: 1.585 ± 0.038
5.973GluIle: 5.973 ± 0.072
6.861GluLys: 6.861 ± 0.071
7.389GluLeu: 7.389 ± 0.078
2.199GluMet: 2.199 ± 0.039
4.184GluAsn: 4.184 ± 0.06
1.847GluPro: 1.847 ± 0.04
3.404GluGln: 3.404 ± 0.05
3.172GluArg: 3.172 ± 0.051
3.66GluSer: 3.66 ± 0.053
3.844GluThr: 3.844 ± 0.057
5.169GluVal: 5.169 ± 0.063
0.831GluTrp: 0.831 ± 0.022
2.493GluTyr: 2.493 ± 0.042
0.0GluXaa: 0.0 ± 0.0
Phe
2.774PheAla: 2.774 ± 0.044
0.366PheCys: 0.366 ± 0.015
2.345PheAsp: 2.345 ± 0.037
2.955PheGlu: 2.955 ± 0.045
2.422PhePhe: 2.422 ± 0.053
3.214PheGly: 3.214 ± 0.057
1.108PheHis: 1.108 ± 0.03
4.284PheIle: 4.284 ± 0.069
2.661PheLys: 2.661 ± 0.04
4.879PheLeu: 4.879 ± 0.072
1.14PheMet: 1.14 ± 0.026
2.077PheAsn: 2.077 ± 0.036
1.673PhePro: 1.673 ± 0.031
1.63PheGln: 1.63 ± 0.032
1.449PheArg: 1.449 ± 0.029
3.395PheSer: 3.395 ± 0.054
2.691PheThr: 2.691 ± 0.042
3.098PheVal: 3.098 ± 0.047
0.501PheTrp: 0.501 ± 0.019
1.792PheTyr: 1.792 ± 0.039
0.0PheXaa: 0.0 ± 0.0
Gly
4.247GlyAla: 4.247 ± 0.078
0.618GlyCys: 0.618 ± 0.021
3.06GlyAsp: 3.06 ± 0.055
4.389GlyGlu: 4.389 ± 0.052
3.367GlyPhe: 3.367 ± 0.052
4.425GlyGly: 4.425 ± 0.074
1.345GlyHis: 1.345 ± 0.029
5.98GlyIle: 5.98 ± 0.066
4.856GlyLys: 4.856 ± 0.06
6.281GlyLeu: 6.281 ± 0.082
1.936GlyMet: 1.936 ± 0.037
2.817GlyAsn: 2.817 ± 0.047
1.652GlyPro: 1.652 ± 0.034
2.11GlyGln: 2.11 ± 0.043
2.313GlyArg: 2.313 ± 0.04
3.908GlySer: 3.908 ± 0.052
3.788GlyThr: 3.788 ± 0.052
4.768GlyVal: 4.768 ± 0.052
0.763GlyTrp: 0.763 ± 0.023
2.765GlyTyr: 2.765 ± 0.041
0.0GlyXaa: 0.0 ± 0.0
His
1.194HisAla: 1.194 ± 0.03
0.192HisCys: 0.192 ± 0.011
1.084HisAsp: 1.084 ± 0.028
1.477HisGlu: 1.477 ± 0.034
1.12HisPhe: 1.12 ± 0.027
1.299HisGly: 1.299 ± 0.029
0.707HisHis: 0.707 ± 0.026
1.615HisIle: 1.615 ± 0.036
1.178HisLys: 1.178 ± 0.031
2.103HisLeu: 2.103 ± 0.037
0.522HisMet: 0.522 ± 0.019
0.904HisAsn: 0.904 ± 0.025
1.069HisPro: 1.069 ± 0.03
0.917HisGln: 0.917 ± 0.024
0.843HisArg: 0.843 ± 0.021
1.369HisSer: 1.369 ± 0.033
1.092HisThr: 1.092 ± 0.025
1.432HisVal: 1.432 ± 0.03
0.237HisTrp: 0.237 ± 0.01
0.964HisTyr: 0.964 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
5.474IleAla: 5.474 ± 0.071
0.715IleCys: 0.715 ± 0.022
4.63IleAsp: 4.63 ± 0.059
6.193IleGlu: 6.193 ± 0.075
3.655IlePhe: 3.655 ± 0.069
6.071IleGly: 6.071 ± 0.079
1.789IleHis: 1.789 ± 0.034
6.508IleIle: 6.508 ± 0.097
5.16IleLys: 5.16 ± 0.059
7.558IleLeu: 7.558 ± 0.085
1.972IleMet: 1.972 ± 0.037
3.868IleAsn: 3.868 ± 0.051
3.452IlePro: 3.452 ± 0.046
3.061IleGln: 3.061 ± 0.047
2.951IleArg: 2.951 ± 0.047
5.628IleSer: 5.628 ± 0.064
4.678IleThr: 4.678 ± 0.053
5.732IleVal: 5.732 ± 0.06
0.71IleTrp: 0.71 ± 0.023
2.659IleTyr: 2.659 ± 0.044
0.0IleXaa: 0.0 ± 0.0
Lys
4.358LysAla: 4.358 ± 0.058
0.345LysCys: 0.345 ± 0.016
3.997LysAsp: 3.997 ± 0.051
7.284LysGlu: 7.284 ± 0.082
2.041LysPhe: 2.041 ± 0.035
4.572LysGly: 4.572 ± 0.061
1.45LysHis: 1.45 ± 0.031
5.165LysIle: 5.165 ± 0.049
6.182LysLys: 6.182 ± 0.08
6.215LysLeu: 6.215 ± 0.068
2.189LysMet: 2.189 ± 0.034
3.774LysAsn: 3.774 ± 0.061
2.19LysPro: 2.19 ± 0.043
3.224LysGln: 3.224 ± 0.049
3.198LysArg: 3.198 ± 0.048
3.882LysSer: 3.882 ± 0.045
3.76LysThr: 3.76 ± 0.053
4.926LysVal: 4.926 ± 0.057
0.848LysTrp: 0.848 ± 0.025
2.336LysTyr: 2.336 ± 0.041
0.0LysXaa: 0.0 ± 0.0
Leu
6.564LeuAla: 6.564 ± 0.073
0.685LeuCys: 0.685 ± 0.022
4.903LeuAsp: 4.903 ± 0.064
6.586LeuGlu: 6.586 ± 0.075
4.98LeuPhe: 4.98 ± 0.067
6.117LeuGly: 6.117 ± 0.07
2.013LeuHis: 2.013 ± 0.041
7.634LeuIle: 7.634 ± 0.093
6.863LeuLys: 6.863 ± 0.066
10.204LeuLeu: 10.204 ± 0.116
2.364LeuMet: 2.364 ± 0.041
4.626LeuAsn: 4.626 ± 0.054
3.766LeuPro: 3.766 ± 0.051
3.628LeuGln: 3.628 ± 0.058
3.423LeuArg: 3.423 ± 0.053
7.233LeuSer: 7.233 ± 0.076
5.781LeuThr: 5.781 ± 0.063
6.298LeuVal: 6.298 ± 0.067
0.823LeuTrp: 0.823 ± 0.025
3.326LeuTyr: 3.326 ± 0.042
0.0LeuXaa: 0.0 ± 0.0
Met
1.732MetAla: 1.732 ± 0.034
0.154MetCys: 0.154 ± 0.011
1.326MetAsp: 1.326 ± 0.03
1.857MetGlu: 1.857 ± 0.038
1.082MetPhe: 1.082 ± 0.022
1.631MetGly: 1.631 ± 0.033
0.379MetHis: 0.379 ± 0.017
2.294MetIle: 2.294 ± 0.042
2.552MetLys: 2.552 ± 0.043
2.509MetLeu: 2.509 ± 0.04
0.836MetMet: 0.836 ± 0.022
1.621MetAsn: 1.621 ± 0.029
0.925MetPro: 0.925 ± 0.025
0.795MetGln: 0.795 ± 0.026
0.979MetArg: 0.979 ± 0.026
1.728MetSer: 1.728 ± 0.035
1.57MetThr: 1.57 ± 0.026
1.735MetVal: 1.735 ± 0.034
0.188MetTrp: 0.188 ± 0.01
0.77MetTyr: 0.77 ± 0.024
0.0MetXaa: 0.0 ± 0.0
Asn
2.522AsnAla: 2.522 ± 0.044
0.343AsnCys: 0.343 ± 0.015
2.466AsnAsp: 2.466 ± 0.04
4.114AsnGlu: 4.114 ± 0.053
1.761AsnPhe: 1.761 ± 0.027
3.415AsnGly: 3.415 ± 0.057
1.117AsnHis: 1.117 ± 0.03
3.869AsnIle: 3.869 ± 0.046
3.5AsnLys: 3.5 ± 0.046
4.091AsnLeu: 4.091 ± 0.056
1.259AsnMet: 1.259 ± 0.027
2.379AsnAsn: 2.379 ± 0.049
2.153AsnPro: 2.153 ± 0.039
2.204AsnGln: 2.204 ± 0.039
2.055AsnArg: 2.055 ± 0.037
2.778AsnSer: 2.778 ± 0.041
2.399AsnThr: 2.399 ± 0.043
3.228AsnVal: 3.228 ± 0.046
0.613AsnTrp: 0.613 ± 0.022
1.743AsnTyr: 1.743 ± 0.041
0.0AsnXaa: 0.0 ± 0.0
Pro
1.999ProAla: 1.999 ± 0.04
0.221ProCys: 0.221 ± 0.012
1.928ProAsp: 1.928 ± 0.036
2.791ProGlu: 2.791 ± 0.049
2.018ProPhe: 2.018 ± 0.038
2.061ProGly: 2.061 ± 0.041
0.774ProHis: 0.774 ± 0.023
2.997ProIle: 2.997 ± 0.045
2.167ProLys: 2.167 ± 0.037
3.402ProLeu: 3.402 ± 0.05
0.826ProMet: 0.826 ± 0.024
1.784ProAsn: 1.784 ± 0.035
0.906ProPro: 0.906 ± 0.028
1.074ProGln: 1.074 ± 0.023
0.983ProArg: 0.983 ± 0.027
2.256ProSer: 2.256 ± 0.038
1.988ProThr: 1.988 ± 0.033
2.566ProVal: 2.566 ± 0.045
0.386ProTrp: 0.386 ± 0.017
1.417ProTyr: 1.417 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
2.482GlnAla: 2.482 ± 0.038
0.211GlnCys: 0.211 ± 0.012
1.797GlnAsp: 1.797 ± 0.034
2.951GlnGlu: 2.951 ± 0.045
1.698GlnPhe: 1.698 ± 0.034
2.077GlnGly: 2.077 ± 0.034
0.926GlnHis: 0.926 ± 0.025
2.757GlnIle: 2.757 ± 0.044
2.868GlnLys: 2.868 ± 0.047
4.174GlnLeu: 4.174 ± 0.059
1.018GlnMet: 1.018 ± 0.029
1.79GlnAsn: 1.79 ± 0.033
1.159GlnPro: 1.159 ± 0.03
1.925GlnGln: 1.925 ± 0.063
1.381GlnArg: 1.381 ± 0.031
2.303GlnSer: 2.303 ± 0.049
2.087GlnThr: 2.087 ± 0.039
2.372GlnVal: 2.372 ± 0.036
0.406GlnTrp: 0.406 ± 0.015
1.472GlnTyr: 1.472 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
2.104ArgAla: 2.104 ± 0.038
0.231ArgCys: 0.231 ± 0.012
1.983ArgAsp: 1.983 ± 0.04
2.993ArgGlu: 2.993 ± 0.044
1.78ArgPhe: 1.78 ± 0.033
2.131ArgGly: 2.131 ± 0.046
0.73ArgHis: 0.73 ± 0.018
2.856ArgIle: 2.856 ± 0.043
3.117ArgLys: 3.117 ± 0.049
3.589ArgLeu: 3.589 ± 0.042
1.157ArgMet: 1.157 ± 0.028
1.948ArgAsn: 1.948 ± 0.035
1.184ArgPro: 1.184 ± 0.032
1.358ArgGln: 1.358 ± 0.031
1.607ArgArg: 1.607 ± 0.035
2.139ArgSer: 2.139 ± 0.039
1.886ArgThr: 1.886 ± 0.035
2.416ArgVal: 2.416 ± 0.041
0.362ArgTrp: 0.362 ± 0.016
1.523ArgTyr: 1.523 ± 0.03
0.0ArgXaa: 0.0 ± 0.0
Ser
3.539SerAla: 3.539 ± 0.052
0.449SerCys: 0.449 ± 0.016
2.974SerAsp: 2.974 ± 0.051
4.27SerGlu: 4.27 ± 0.054
3.515SerPhe: 3.515 ± 0.047
4.181SerGly: 4.181 ± 0.056
1.252SerHis: 1.252 ± 0.031
5.727SerIle: 5.727 ± 0.065
4.254SerLys: 4.254 ± 0.057
6.472SerLeu: 6.472 ± 0.075
1.706SerMet: 1.706 ± 0.036
3.024SerAsn: 3.024 ± 0.045
2.096SerPro: 2.096 ± 0.033
2.209SerGln: 2.209 ± 0.038
2.163SerArg: 2.163 ± 0.038
4.434SerSer: 4.434 ± 0.069
3.492SerThr: 3.492 ± 0.051
4.157SerVal: 4.157 ± 0.054
0.66SerTrp: 0.66 ± 0.018
2.486SerTyr: 2.486 ± 0.043
0.0SerXaa: 0.0 ± 0.0
Thr
3.531ThrAla: 3.531 ± 0.051
0.41ThrCys: 0.41 ± 0.017
2.732ThrAsp: 2.732 ± 0.049
3.586ThrGlu: 3.586 ± 0.056
2.803ThrPhe: 2.803 ± 0.05
3.922ThrGly: 3.922 ± 0.059
1.019ThrHis: 1.019 ± 0.026
4.944ThrIle: 4.944 ± 0.059
3.578ThrLys: 3.578 ± 0.053
5.32ThrLeu: 5.32 ± 0.062
1.252ThrMet: 1.252 ± 0.03
2.734ThrAsn: 2.734 ± 0.04
2.226ThrPro: 2.226 ± 0.041
1.505ThrGln: 1.505 ± 0.034
1.752ThrArg: 1.752 ± 0.031
3.671ThrSer: 3.671 ± 0.047
3.039ThrThr: 3.039 ± 0.05
3.95ThrVal: 3.95 ± 0.051
0.572ThrTrp: 0.572 ± 0.02
2.091ThrTyr: 2.091 ± 0.04
0.0ThrXaa: 0.0 ± 0.0
Val
4.546ValAla: 4.546 ± 0.061
0.568ValCys: 0.568 ± 0.02
3.619ValAsp: 3.619 ± 0.048
5.009ValGlu: 5.009 ± 0.07
3.107ValPhe: 3.107 ± 0.049
4.47ValGly: 4.47 ± 0.063
1.33ValHis: 1.33 ± 0.03
5.699ValIle: 5.699 ± 0.061
4.903ValLys: 4.903 ± 0.057
6.562ValLeu: 6.562 ± 0.068
1.76ValMet: 1.76 ± 0.033
3.294ValAsn: 3.294 ± 0.041
2.506ValPro: 2.506 ± 0.041
2.338ValGln: 2.338 ± 0.035
2.403ValArg: 2.403 ± 0.041
4.627ValSer: 4.627 ± 0.051
3.992ValThr: 3.992 ± 0.052
4.885ValVal: 4.885 ± 0.058
0.621ValTrp: 0.621 ± 0.022
2.347ValTyr: 2.347 ± 0.041
0.0ValXaa: 0.0 ± 0.0
Trp
0.583TrpAla: 0.583 ± 0.022
0.093TrpCys: 0.093 ± 0.007
0.55TrpAsp: 0.55 ± 0.021
0.7TrpGlu: 0.7 ± 0.023
0.536TrpPhe: 0.536 ± 0.017
0.685TrpGly: 0.685 ± 0.022
0.217TrpHis: 0.217 ± 0.013
0.891TrpIle: 0.891 ± 0.023
0.782TrpLys: 0.782 ± 0.025
1.103TrpLeu: 1.103 ± 0.028
0.346TrpMet: 0.346 ± 0.015
0.611TrpAsn: 0.611 ± 0.02
0.228TrpPro: 0.228 ± 0.012
0.361TrpGln: 0.361 ± 0.016
0.377TrpArg: 0.377 ± 0.016
0.639TrpSer: 0.639 ± 0.023
0.519TrpThr: 0.519 ± 0.017
0.645TrpVal: 0.645 ± 0.02
0.142TrpTrp: 0.142 ± 0.011
0.384TrpTyr: 0.384 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.944TyrAla: 1.944 ± 0.04
0.33TyrCys: 0.33 ± 0.014
1.992TyrAsp: 1.992 ± 0.039
2.669TyrGlu: 2.669 ± 0.039
1.975TyrPhe: 1.975 ± 0.037
2.514TyrGly: 2.514 ± 0.038
0.902TyrHis: 0.902 ± 0.024
2.732TyrIle: 2.732 ± 0.046
2.42TyrLys: 2.42 ± 0.043
3.665TyrLeu: 3.665 ± 0.054
0.887TyrMet: 0.887 ± 0.023
1.65TyrAsn: 1.65 ± 0.033
1.395TyrPro: 1.395 ± 0.033
1.621TyrGln: 1.621 ± 0.032
1.542TyrArg: 1.542 ± 0.035
2.356TyrSer: 2.356 ± 0.041
1.897TyrThr: 1.897 ± 0.037
2.289TyrVal: 2.289 ± 0.039
0.402TyrTrp: 0.402 ± 0.017
1.523TyrTyr: 1.523 ± 0.029
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5279 proteins (1537910 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski