Amino acid dipepetide frequency for Bacillus smithii

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.109AlaAla: 6.109 ± 0.118
0.615AlaCys: 0.615 ± 0.026
3.37AlaAsp: 3.37 ± 0.065
4.7AlaGlu: 4.7 ± 0.083
3.361AlaPhe: 3.361 ± 0.073
5.205AlaGly: 5.205 ± 0.092
1.292AlaHis: 1.292 ± 0.041
5.64AlaIle: 5.64 ± 0.09
5.092AlaLys: 5.092 ± 0.075
6.978AlaLeu: 6.978 ± 0.108
1.91AlaMet: 1.91 ± 0.048
2.699AlaAsn: 2.699 ± 0.053
2.013AlaPro: 2.013 ± 0.056
2.108AlaGln: 2.108 ± 0.056
2.9AlaArg: 2.9 ± 0.054
3.884AlaSer: 3.884 ± 0.078
2.917AlaThr: 2.917 ± 0.066
5.686AlaVal: 5.686 ± 0.102
0.522AlaTrp: 0.522 ± 0.027
2.258AlaTyr: 2.258 ± 0.055
0.0AlaXaa: 0.0 ± 0.0
Cys
0.473CysAla: 0.473 ± 0.025
0.128CysCys: 0.128 ± 0.012
0.371CysAsp: 0.371 ± 0.02
0.507CysGlu: 0.507 ± 0.026
0.424CysPhe: 0.424 ± 0.023
0.743CysGly: 0.743 ± 0.032
0.238CysHis: 0.238 ± 0.017
0.537CysIle: 0.537 ± 0.025
0.403CysLys: 0.403 ± 0.024
0.831CysLeu: 0.831 ± 0.029
0.201CysMet: 0.201 ± 0.015
0.249CysAsn: 0.249 ± 0.018
0.421CysPro: 0.421 ± 0.025
0.29CysGln: 0.29 ± 0.02
0.368CysArg: 0.368 ± 0.02
0.568CysSer: 0.568 ± 0.029
0.39CysThr: 0.39 ± 0.022
0.425CysVal: 0.425 ± 0.024
0.075CysTrp: 0.075 ± 0.008
0.293CysTyr: 0.293 ± 0.018
0.0CysXaa: 0.0 ± 0.0
Asp
2.985AspAla: 2.985 ± 0.068
0.442AspCys: 0.442 ± 0.025
2.195AspAsp: 2.195 ± 0.054
4.041AspGlu: 4.041 ± 0.077
2.37AspPhe: 2.37 ± 0.059
3.135AspGly: 3.135 ± 0.065
1.432AspHis: 1.432 ± 0.043
3.849AspIle: 3.849 ± 0.079
2.765AspLys: 2.765 ± 0.072
4.864AspLeu: 4.864 ± 0.087
1.208AspMet: 1.208 ± 0.035
1.326AspAsn: 1.326 ± 0.044
2.26AspPro: 2.26 ± 0.042
2.126AspGln: 2.126 ± 0.05
2.459AspArg: 2.459 ± 0.054
2.502AspSer: 2.502 ± 0.057
2.018AspThr: 2.018 ± 0.049
3.492AspVal: 3.492 ± 0.063
0.629AspTrp: 0.629 ± 0.027
1.995AspTyr: 1.995 ± 0.051
0.0AspXaa: 0.0 ± 0.0
Glu
5.23GluAla: 5.23 ± 0.077
0.397GluCys: 0.397 ± 0.025
3.347GluAsp: 3.347 ± 0.06
6.963GluGlu: 6.963 ± 0.117
2.523GluPhe: 2.523 ± 0.051
4.133GluGly: 4.133 ± 0.084
1.497GluHis: 1.497 ± 0.049
5.773GluIle: 5.773 ± 0.091
7.843GluLys: 7.843 ± 0.096
6.72GluLeu: 6.72 ± 0.097
2.43GluMet: 2.43 ± 0.06
3.713GluAsn: 3.713 ± 0.068
2.037GluPro: 2.037 ± 0.052
3.26GluGln: 3.26 ± 0.066
3.834GluArg: 3.834 ± 0.072
3.308GluSer: 3.308 ± 0.064
3.813GluThr: 3.813 ± 0.079
4.61GluVal: 4.61 ± 0.085
0.926GluTrp: 0.926 ± 0.034
2.073GluTyr: 2.073 ± 0.054
0.0GluXaa: 0.0 ± 0.0
Phe
3.145PheAla: 3.145 ± 0.064
0.423PheCys: 0.423 ± 0.019
2.179PheAsp: 2.179 ± 0.047
2.629PheGlu: 2.629 ± 0.055
2.692PhePhe: 2.692 ± 0.072
3.309PheGly: 3.309 ± 0.063
1.387PheHis: 1.387 ± 0.042
3.676PheIle: 3.676 ± 0.075
2.162PheLys: 2.162 ± 0.056
5.304PheLeu: 5.304 ± 0.104
1.256PheMet: 1.256 ± 0.036
1.391PheAsn: 1.391 ± 0.035
1.864PhePro: 1.864 ± 0.051
2.044PheGln: 2.044 ± 0.055
1.869PheArg: 1.869 ± 0.046
3.535PheSer: 3.535 ± 0.073
2.332PheThr: 2.332 ± 0.055
3.254PheVal: 3.254 ± 0.071
0.528PheTrp: 0.528 ± 0.028
1.901PheTyr: 1.901 ± 0.057
0.0PheXaa: 0.0 ± 0.0
Gly
4.657GlyAla: 4.657 ± 0.088
0.668GlyCys: 0.668 ± 0.028
3.044GlyAsp: 3.044 ± 0.064
4.193GlyGlu: 4.193 ± 0.062
3.378GlyPhe: 3.378 ± 0.069
4.59GlyGly: 4.59 ± 0.101
1.423GlyHis: 1.423 ± 0.047
6.07GlyIle: 6.07 ± 0.088
5.627GlyLys: 5.627 ± 0.094
6.409GlyLeu: 6.409 ± 0.091
2.093GlyMet: 2.093 ± 0.047
2.533GlyAsn: 2.533 ± 0.061
1.763GlyPro: 1.763 ± 0.051
2.166GlyGln: 2.166 ± 0.051
2.944GlyArg: 2.944 ± 0.068
3.765GlySer: 3.765 ± 0.074
3.987GlyThr: 3.987 ± 0.07
4.84GlyVal: 4.84 ± 0.073
0.773GlyTrp: 0.773 ± 0.036
2.68GlyTyr: 2.68 ± 0.059
0.0GlyXaa: 0.0 ± 0.0
His
1.267HisAla: 1.267 ± 0.043
0.27HisCys: 0.27 ± 0.017
1.069HisAsp: 1.069 ± 0.033
1.471HisGlu: 1.471 ± 0.041
1.315HisPhe: 1.315 ± 0.042
1.43HisGly: 1.43 ± 0.044
0.833HisHis: 0.833 ± 0.037
1.668HisIle: 1.668 ± 0.046
1.162HisLys: 1.162 ± 0.033
2.383HisLeu: 2.383 ± 0.064
0.543HisMet: 0.543 ± 0.026
0.748HisAsn: 0.748 ± 0.03
1.426HisPro: 1.426 ± 0.041
1.01HisGln: 1.01 ± 0.033
1.04HisArg: 1.04 ± 0.033
1.498HisSer: 1.498 ± 0.042
1.038HisThr: 1.038 ± 0.037
1.535HisVal: 1.535 ± 0.042
0.279HisTrp: 0.279 ± 0.017
0.972HisTyr: 0.972 ± 0.035
0.0HisXaa: 0.0 ± 0.0
Ile
5.878IleAla: 5.878 ± 0.097
0.701IleCys: 0.701 ± 0.029
3.985IleAsp: 3.985 ± 0.069
5.473IleGlu: 5.473 ± 0.091
3.382IlePhe: 3.382 ± 0.077
6.037IleGly: 6.037 ± 0.101
2.074IleHis: 2.074 ± 0.05
5.433IleIle: 5.433 ± 0.095
4.462IleLys: 4.462 ± 0.081
7.233IleLeu: 7.233 ± 0.105
1.747IleMet: 1.747 ± 0.044
2.616IleAsn: 2.616 ± 0.048
3.532IlePro: 3.532 ± 0.063
3.429IleGln: 3.429 ± 0.062
3.797IleArg: 3.797 ± 0.068
4.931IleSer: 4.931 ± 0.087
3.767IleThr: 3.767 ± 0.066
5.722IleVal: 5.722 ± 0.106
0.677IleTrp: 0.677 ± 0.029
2.365IleTyr: 2.365 ± 0.056
0.0IleXaa: 0.0 ± 0.0
Lys
5.212LysAla: 5.212 ± 0.085
0.361LysCys: 0.361 ± 0.021
3.746LysAsp: 3.746 ± 0.075
7.799LysGlu: 7.799 ± 0.11
2.002LysPhe: 2.002 ± 0.056
5.062LysGly: 5.062 ± 0.087
1.322LysHis: 1.322 ± 0.045
5.309LysIle: 5.309 ± 0.084
7.362LysLys: 7.362 ± 0.138
5.964LysLeu: 5.964 ± 0.09
2.495LysMet: 2.495 ± 0.061
3.596LysAsn: 3.596 ± 0.065
2.428LysPro: 2.428 ± 0.056
3.248LysGln: 3.248 ± 0.063
3.998LysArg: 3.998 ± 0.07
3.422LysSer: 3.422 ± 0.062
3.994LysThr: 3.994 ± 0.061
4.723LysVal: 4.723 ± 0.085
0.976LysTrp: 0.976 ± 0.032
2.147LysTyr: 2.147 ± 0.061
0.0LysXaa: 0.0 ± 0.0
Leu
7.294LeuAla: 7.294 ± 0.098
0.734LeuCys: 0.734 ± 0.03
4.723LeuAsp: 4.723 ± 0.082
6.846LeuGlu: 6.846 ± 0.09
5.207LeuPhe: 5.207 ± 0.11
6.026LeuGly: 6.026 ± 0.105
2.104LeuHis: 2.104 ± 0.05
6.825LeuIle: 6.825 ± 0.106
7.506LeuLys: 7.506 ± 0.115
10.291LeuLeu: 10.291 ± 0.138
2.517LeuMet: 2.517 ± 0.062
4.08LeuAsn: 4.08 ± 0.073
4.152LeuPro: 4.152 ± 0.073
3.538LeuGln: 3.538 ± 0.073
3.838LeuArg: 3.838 ± 0.085
6.787LeuSer: 6.787 ± 0.098
5.159LeuThr: 5.159 ± 0.088
6.012LeuVal: 6.012 ± 0.098
0.884LeuTrp: 0.884 ± 0.036
3.274LeuTyr: 3.274 ± 0.07
0.0LeuXaa: 0.0 ± 0.0
Met
2.183MetAla: 2.183 ± 0.049
0.186MetCys: 0.186 ± 0.015
1.618MetAsp: 1.618 ± 0.042
2.215MetGlu: 2.215 ± 0.051
1.071MetPhe: 1.071 ± 0.04
1.784MetGly: 1.784 ± 0.048
0.403MetHis: 0.403 ± 0.022
2.341MetIle: 2.341 ± 0.055
2.739MetLys: 2.739 ± 0.052
2.307MetLeu: 2.307 ± 0.052
0.966MetMet: 0.966 ± 0.041
1.523MetAsn: 1.523 ± 0.044
0.977MetPro: 0.977 ± 0.033
0.719MetGln: 0.719 ± 0.029
1.137MetArg: 1.137 ± 0.03
1.528MetSer: 1.528 ± 0.047
1.613MetThr: 1.613 ± 0.044
1.73MetVal: 1.73 ± 0.046
0.169MetTrp: 0.169 ± 0.015
0.659MetTyr: 0.659 ± 0.028
0.0MetXaa: 0.0 ± 0.0
Asn
2.545AsnAla: 2.545 ± 0.055
0.315AsnCys: 0.315 ± 0.016
1.944AsnAsp: 1.944 ± 0.051
3.299AsnGlu: 3.299 ± 0.067
1.393AsnPhe: 1.393 ± 0.041
3.476AsnGly: 3.476 ± 0.064
1.107AsnHis: 1.107 ± 0.04
3.148AsnIle: 3.148 ± 0.057
2.53AsnLys: 2.53 ± 0.057
3.52AsnLeu: 3.52 ± 0.064
1.119AsnMet: 1.119 ± 0.036
1.505AsnAsn: 1.505 ± 0.051
2.084AsnPro: 2.084 ± 0.054
1.893AsnGln: 1.893 ± 0.055
2.198AsnArg: 2.198 ± 0.062
2.148AsnSer: 2.148 ± 0.058
1.833AsnThr: 1.833 ± 0.05
2.76AsnVal: 2.76 ± 0.054
0.509AsnTrp: 0.509 ± 0.024
1.237AsnTyr: 1.237 ± 0.04
0.0AsnXaa: 0.0 ± 0.0
Pro
2.422ProAla: 2.422 ± 0.045
0.256ProCys: 0.256 ± 0.015
2.193ProAsp: 2.193 ± 0.053
3.102ProGlu: 3.102 ± 0.065
2.285ProPhe: 2.285 ± 0.057
2.407ProGly: 2.407 ± 0.059
0.9ProHis: 0.9 ± 0.033
2.958ProIle: 2.958 ± 0.062
2.586ProLys: 2.586 ± 0.057
3.756ProLeu: 3.756 ± 0.062
0.836ProMet: 0.836 ± 0.033
1.796ProAsn: 1.796 ± 0.054
1.18ProPro: 1.18 ± 0.038
1.202ProGln: 1.202 ± 0.037
1.227ProArg: 1.227 ± 0.042
2.459ProSer: 2.459 ± 0.058
1.836ProThr: 1.836 ± 0.046
3.068ProVal: 3.068 ± 0.058
0.392ProTrp: 0.392 ± 0.024
1.53ProTyr: 1.53 ± 0.046
0.0ProXaa: 0.0 ± 0.0
Gln
2.562GlnAla: 2.562 ± 0.062
0.251GlnCys: 0.251 ± 0.017
1.481GlnAsp: 1.481 ± 0.045
2.881GlnGlu: 2.881 ± 0.061
1.735GlnPhe: 1.735 ± 0.045
2.006GlnGly: 2.006 ± 0.045
0.795GlnHis: 0.795 ± 0.029
2.719GlnIle: 2.719 ± 0.06
3.463GlnLys: 3.463 ± 0.072
4.197GlnLeu: 4.197 ± 0.078
1.25GlnMet: 1.25 ± 0.038
1.817GlnAsn: 1.817 ± 0.047
1.388GlnPro: 1.388 ± 0.046
1.763GlnGln: 1.763 ± 0.053
1.565GlnArg: 1.565 ± 0.041
2.296GlnSer: 2.296 ± 0.045
2.1GlnThr: 2.1 ± 0.052
2.169GlnVal: 2.169 ± 0.05
0.451GlnTrp: 0.451 ± 0.027
1.435GlnTyr: 1.435 ± 0.045
0.0GlnXaa: 0.0 ± 0.0
Arg
2.521ArgAla: 2.521 ± 0.054
0.331ArgCys: 0.331 ± 0.018
2.027ArgAsp: 2.027 ± 0.055
3.423ArgGlu: 3.423 ± 0.07
2.292ArgPhe: 2.292 ± 0.052
2.478ArgGly: 2.478 ± 0.054
1.07ArgHis: 1.07 ± 0.037
3.333ArgIle: 3.333 ± 0.071
4.008ArgLys: 4.008 ± 0.064
4.651ArgLeu: 4.651 ± 0.07
1.502ArgMet: 1.502 ± 0.041
2.004ArgAsn: 2.004 ± 0.052
1.612ArgPro: 1.612 ± 0.049
1.731ArgGln: 1.731 ± 0.046
2.232ArgArg: 2.232 ± 0.06
2.527ArgSer: 2.527 ± 0.057
2.176ArgThr: 2.176 ± 0.053
2.636ArgVal: 2.636 ± 0.056
0.498ArgTrp: 0.498 ± 0.027
1.728ArgTyr: 1.728 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
3.703SerAla: 3.703 ± 0.07
0.492SerCys: 0.492 ± 0.028
2.662SerAsp: 2.662 ± 0.053
3.68SerGlu: 3.68 ± 0.059
3.375SerPhe: 3.375 ± 0.071
4.206SerGly: 4.206 ± 0.08
1.38SerHis: 1.38 ± 0.041
4.785SerIle: 4.785 ± 0.076
3.985SerLys: 3.985 ± 0.077
6.167SerLeu: 6.167 ± 0.099
1.585SerMet: 1.585 ± 0.04
2.249SerAsn: 2.249 ± 0.053
2.332SerPro: 2.332 ± 0.052
2.081SerGln: 2.081 ± 0.05
2.526SerArg: 2.526 ± 0.068
3.888SerSer: 3.888 ± 0.083
2.85SerThr: 2.85 ± 0.065
4.169SerVal: 4.169 ± 0.07
0.613SerTrp: 0.613 ± 0.023
2.149SerTyr: 2.149 ± 0.053
0.0SerXaa: 0.0 ± 0.0
Thr
3.802ThrAla: 3.802 ± 0.062
0.345ThrCys: 0.345 ± 0.021
2.374ThrAsp: 2.374 ± 0.053
3.198ThrGlu: 3.198 ± 0.066
2.472ThrPhe: 2.472 ± 0.053
3.994ThrGly: 3.994 ± 0.073
0.996ThrHis: 0.996 ± 0.033
4.404ThrIle: 4.404 ± 0.07
3.491ThrLys: 3.491 ± 0.069
4.633ThrLeu: 4.633 ± 0.088
1.254ThrMet: 1.254 ± 0.038
2.174ThrAsn: 2.174 ± 0.059
2.232ThrPro: 2.232 ± 0.053
1.18ThrGln: 1.18 ± 0.035
1.809ThrArg: 1.809 ± 0.049
2.882ThrSer: 2.882 ± 0.062
2.569ThrThr: 2.569 ± 0.059
4.133ThrVal: 4.133 ± 0.072
0.453ThrTrp: 0.453 ± 0.024
1.722ThrTyr: 1.722 ± 0.046
0.0ThrXaa: 0.0 ± 0.0
Val
4.711ValAla: 4.711 ± 0.089
0.602ValCys: 0.602 ± 0.029
3.488ValAsp: 3.488 ± 0.066
4.757ValGlu: 4.757 ± 0.083
3.246ValPhe: 3.246 ± 0.066
4.384ValGly: 4.384 ± 0.076
1.541ValHis: 1.541 ± 0.047
5.515ValIle: 5.515 ± 0.084
4.955ValLys: 4.955 ± 0.081
6.936ValLeu: 6.936 ± 0.102
1.75ValMet: 1.75 ± 0.052
2.774ValAsn: 2.774 ± 0.054
2.904ValPro: 2.904 ± 0.063
2.586ValGln: 2.586 ± 0.053
2.874ValArg: 2.874 ± 0.066
4.346ValSer: 4.346 ± 0.07
3.529ValThr: 3.529 ± 0.066
4.99ValVal: 4.99 ± 0.091
0.624ValTrp: 0.624 ± 0.028
2.269ValTyr: 2.269 ± 0.053
0.0ValXaa: 0.0 ± 0.0
Trp
0.603TrpAla: 0.603 ± 0.03
0.083TrpCys: 0.083 ± 0.01
0.495TrpAsp: 0.495 ± 0.024
0.721TrpGlu: 0.721 ± 0.029
0.523TrpPhe: 0.523 ± 0.024
0.652TrpGly: 0.652 ± 0.029
0.222TrpHis: 0.222 ± 0.016
0.963TrpIle: 0.963 ± 0.033
0.965TrpLys: 0.965 ± 0.032
1.201TrpLeu: 1.201 ± 0.041
0.377TrpMet: 0.377 ± 0.019
0.567TrpAsn: 0.567 ± 0.025
0.291TrpPro: 0.291 ± 0.018
0.351TrpGln: 0.351 ± 0.021
0.433TrpArg: 0.433 ± 0.021
0.558TrpSer: 0.558 ± 0.025
0.54TrpThr: 0.54 ± 0.028
0.59TrpVal: 0.59 ± 0.026
0.123TrpTrp: 0.123 ± 0.013
0.308TrpTyr: 0.308 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.958TyrAla: 1.958 ± 0.054
0.335TyrCys: 0.335 ± 0.02
1.783TyrAsp: 1.783 ± 0.043
2.533TyrGlu: 2.533 ± 0.059
1.834TyrPhe: 1.834 ± 0.054
2.545TyrGly: 2.545 ± 0.061
0.941TyrHis: 0.941 ± 0.034
2.293TyrIle: 2.293 ± 0.057
2.058TyrLys: 2.058 ± 0.061
3.45TyrLeu: 3.45 ± 0.063
0.828TyrMet: 0.828 ± 0.033
1.203TyrAsn: 1.203 ± 0.04
1.494TyrPro: 1.494 ± 0.046
1.598TyrGln: 1.598 ± 0.048
1.734TyrArg: 1.734 ± 0.047
2.046TyrSer: 2.046 ± 0.052
1.656TyrThr: 1.656 ± 0.039
2.236TyrVal: 2.236 ± 0.051
0.468TyrTrp: 0.468 ± 0.024
1.374TyrTyr: 1.374 ± 0.044
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3481 proteins (854256 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski