Amino acid dipepetide frequency for Escherichia phage CICC 80001

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.991AlaAla: 8.991 ± 1.455
0.865AlaCys: 0.865 ± 0.264
6.225AlaAsp: 6.225 ± 0.817
4.928AlaGlu: 4.928 ± 0.609
2.939AlaPhe: 2.939 ± 0.42
7.521AlaGly: 7.521 ± 1.013
1.816AlaHis: 1.816 ± 0.356
5.274AlaIle: 5.274 ± 0.983
7.262AlaLys: 7.262 ± 0.753
8.299AlaLeu: 8.299 ± 1.078
3.199AlaMet: 3.199 ± 0.627
3.977AlaAsn: 3.977 ± 0.484
2.248AlaPro: 2.248 ± 0.314
3.372AlaGln: 3.372 ± 0.739
5.447AlaArg: 5.447 ± 0.571
5.533AlaSer: 5.533 ± 0.75
3.804AlaThr: 3.804 ± 0.539
5.101AlaVal: 5.101 ± 0.683
1.816AlaTrp: 1.816 ± 0.405
2.853AlaTyr: 2.853 ± 0.526
0.0AlaXaa: 0.0 ± 0.0
Cys
0.865CysAla: 0.865 ± 0.278
0.086CysCys: 0.086 ± 0.071
1.21CysAsp: 1.21 ± 0.431
0.778CysGlu: 0.778 ± 0.32
0.605CysPhe: 0.605 ± 0.246
0.692CysGly: 0.692 ± 0.323
0.605CysHis: 0.605 ± 0.288
0.692CysIle: 0.692 ± 0.248
0.605CysLys: 0.605 ± 0.25
0.778CysLeu: 0.778 ± 0.257
0.259CysMet: 0.259 ± 0.202
0.173CysAsn: 0.173 ± 0.118
0.346CysPro: 0.346 ± 0.185
0.432CysGln: 0.432 ± 0.175
0.432CysArg: 0.432 ± 0.205
0.692CysSer: 0.692 ± 0.223
0.346CysThr: 0.346 ± 0.182
0.951CysVal: 0.951 ± 0.368
0.346CysTrp: 0.346 ± 0.219
0.259CysTyr: 0.259 ± 0.142
0.0CysXaa: 0.0 ± 0.0
Asp
4.409AspAla: 4.409 ± 0.552
0.519AspCys: 0.519 ± 0.207
4.063AspAsp: 4.063 ± 0.592
4.668AspGlu: 4.668 ± 0.589
2.766AspPhe: 2.766 ± 0.373
5.706AspGly: 5.706 ± 0.549
0.692AspHis: 0.692 ± 0.257
3.631AspIle: 3.631 ± 0.459
4.582AspLys: 4.582 ± 0.586
3.977AspLeu: 3.977 ± 0.824
2.594AspMet: 2.594 ± 0.507
2.334AspAsn: 2.334 ± 0.359
3.199AspPro: 3.199 ± 0.636
1.729AspGln: 1.729 ± 0.354
3.026AspArg: 3.026 ± 0.574
4.582AspSer: 4.582 ± 0.716
4.323AspThr: 4.323 ± 0.589
5.187AspVal: 5.187 ± 0.575
1.037AspTrp: 1.037 ± 0.301
1.729AspTyr: 1.729 ± 0.307
0.0AspXaa: 0.0 ± 0.0
Glu
7.348GluAla: 7.348 ± 1.073
0.432GluCys: 0.432 ± 0.19
4.582GluAsp: 4.582 ± 0.798
7.348GluGlu: 7.348 ± 1.216
2.161GluPhe: 2.161 ± 0.361
5.274GluGly: 5.274 ± 0.759
1.988GluHis: 1.988 ± 0.444
3.372GluIle: 3.372 ± 0.456
3.804GluLys: 3.804 ± 0.457
6.225GluLeu: 6.225 ± 0.61
2.421GluMet: 2.421 ± 0.484
2.939GluAsn: 2.939 ± 0.453
1.643GluPro: 1.643 ± 0.426
3.717GluGln: 3.717 ± 1.005
3.717GluArg: 3.717 ± 0.517
4.496GluSer: 4.496 ± 0.741
3.89GluThr: 3.89 ± 0.51
4.755GluVal: 4.755 ± 0.64
1.037GluTrp: 1.037 ± 0.351
3.458GluTyr: 3.458 ± 0.712
0.0GluXaa: 0.0 ± 0.0
Phe
1.988PheAla: 1.988 ± 0.338
0.519PheCys: 0.519 ± 0.2
3.372PheAsp: 3.372 ± 0.553
2.507PheGlu: 2.507 ± 0.455
0.951PhePhe: 0.951 ± 0.246
3.285PheGly: 3.285 ± 0.523
0.605PheHis: 0.605 ± 0.289
1.729PheIle: 1.729 ± 0.426
2.507PheLys: 2.507 ± 0.359
2.853PheLeu: 2.853 ± 0.47
1.297PheMet: 1.297 ± 0.218
1.47PheAsn: 1.47 ± 0.429
1.47PhePro: 1.47 ± 0.396
1.297PheGln: 1.297 ± 0.305
1.729PheArg: 1.729 ± 0.334
1.729PheSer: 1.729 ± 0.46
2.248PheThr: 2.248 ± 0.414
1.902PheVal: 1.902 ± 0.454
0.259PheTrp: 0.259 ± 0.13
1.124PheTyr: 1.124 ± 0.287
0.0PheXaa: 0.0 ± 0.0
Gly
7.089GlyAla: 7.089 ± 0.951
0.865GlyCys: 0.865 ± 0.304
4.496GlyAsp: 4.496 ± 0.622
4.755GlyGlu: 4.755 ± 0.705
3.199GlyPhe: 3.199 ± 0.521
5.274GlyGly: 5.274 ± 0.597
1.47GlyHis: 1.47 ± 0.324
4.755GlyIle: 4.755 ± 0.753
5.619GlyLys: 5.619 ± 0.877
5.792GlyLeu: 5.792 ± 0.89
2.507GlyMet: 2.507 ± 0.494
2.853GlyAsn: 2.853 ± 0.519
0.692GlyPro: 0.692 ± 0.296
2.853GlyGln: 2.853 ± 0.462
4.496GlyArg: 4.496 ± 0.528
4.755GlySer: 4.755 ± 0.622
3.631GlyThr: 3.631 ± 0.576
3.285GlyVal: 3.285 ± 0.429
1.816GlyTrp: 1.816 ± 0.538
2.766GlyTyr: 2.766 ± 0.496
0.0GlyXaa: 0.0 ± 0.0
His
1.643HisAla: 1.643 ± 0.453
0.346HisCys: 0.346 ± 0.154
1.297HisAsp: 1.297 ± 0.294
1.729HisGlu: 1.729 ± 0.371
0.692HisPhe: 0.692 ± 0.199
1.297HisGly: 1.297 ± 0.249
0.605HisHis: 0.605 ± 0.238
1.47HisIle: 1.47 ± 0.381
1.556HisLys: 1.556 ± 0.351
2.075HisLeu: 2.075 ± 0.574
0.519HisMet: 0.519 ± 0.199
0.778HisAsn: 0.778 ± 0.264
0.259HisPro: 0.259 ± 0.143
0.259HisGln: 0.259 ± 0.186
0.692HisArg: 0.692 ± 0.207
1.037HisSer: 1.037 ± 0.249
0.692HisThr: 0.692 ± 0.244
1.297HisVal: 1.297 ± 0.335
0.519HisTrp: 0.519 ± 0.228
1.037HisTyr: 1.037 ± 0.257
0.0HisXaa: 0.0 ± 0.0
Ile
3.89IleAla: 3.89 ± 0.63
0.519IleCys: 0.519 ± 0.176
4.323IleAsp: 4.323 ± 0.675
4.323IleGlu: 4.323 ± 0.612
0.951IlePhe: 0.951 ± 0.202
3.199IleGly: 3.199 ± 0.402
1.383IleHis: 1.383 ± 0.433
3.112IleIle: 3.112 ± 0.617
4.15IleLys: 4.15 ± 0.564
4.063IleLeu: 4.063 ± 0.604
1.297IleMet: 1.297 ± 0.365
2.68IleAsn: 2.68 ± 0.64
2.594IlePro: 2.594 ± 0.383
1.297IleGln: 1.297 ± 0.426
3.458IleArg: 3.458 ± 0.502
2.939IleSer: 2.939 ± 0.506
3.199IleThr: 3.199 ± 0.417
3.026IleVal: 3.026 ± 0.503
0.605IleTrp: 0.605 ± 0.189
1.816IleTyr: 1.816 ± 0.245
0.0IleXaa: 0.0 ± 0.0
Lys
7.867LysAla: 7.867 ± 0.943
0.778LysCys: 0.778 ± 0.298
3.458LysAsp: 3.458 ± 0.484
5.533LysGlu: 5.533 ± 0.779
2.248LysPhe: 2.248 ± 0.375
4.841LysGly: 4.841 ± 0.716
1.816LysHis: 1.816 ± 0.387
2.421LysIle: 2.421 ± 0.455
4.323LysLys: 4.323 ± 1.019
5.187LysLeu: 5.187 ± 0.848
2.075LysMet: 2.075 ± 0.406
2.594LysAsn: 2.594 ± 0.379
2.594LysPro: 2.594 ± 0.537
2.766LysGln: 2.766 ± 0.595
4.323LysArg: 4.323 ± 0.68
4.323LysSer: 4.323 ± 0.494
3.285LysThr: 3.285 ± 0.552
4.582LysVal: 4.582 ± 0.492
0.692LysTrp: 0.692 ± 0.224
2.766LysTyr: 2.766 ± 0.408
0.0LysXaa: 0.0 ± 0.0
Leu
8.645LeuAla: 8.645 ± 1.086
0.432LeuCys: 0.432 ± 0.234
4.15LeuAsp: 4.15 ± 0.562
6.484LeuGlu: 6.484 ± 0.739
2.248LeuPhe: 2.248 ± 0.434
4.323LeuGly: 4.323 ± 0.742
1.383LeuHis: 1.383 ± 0.382
3.545LeuIle: 3.545 ± 0.618
5.619LeuLys: 5.619 ± 0.707
4.668LeuLeu: 4.668 ± 0.719
2.594LeuMet: 2.594 ± 0.451
3.804LeuAsn: 3.804 ± 0.442
3.026LeuPro: 3.026 ± 0.51
3.717LeuGln: 3.717 ± 0.629
5.619LeuArg: 5.619 ± 0.67
4.409LeuSer: 4.409 ± 0.622
4.582LeuThr: 4.582 ± 0.526
3.977LeuVal: 3.977 ± 0.639
1.47LeuTrp: 1.47 ± 0.428
2.766LeuTyr: 2.766 ± 0.575
0.0LeuXaa: 0.0 ± 0.0
Met
3.199MetAla: 3.199 ± 0.491
0.432MetCys: 0.432 ± 0.208
1.988MetAsp: 1.988 ± 0.432
1.902MetGlu: 1.902 ± 0.44
1.124MetPhe: 1.124 ± 0.303
2.421MetGly: 2.421 ± 0.434
0.519MetHis: 0.519 ± 0.209
1.47MetIle: 1.47 ± 0.349
1.21MetLys: 1.21 ± 0.293
3.631MetLeu: 3.631 ± 0.551
0.865MetMet: 0.865 ± 0.228
1.21MetAsn: 1.21 ± 0.349
1.297MetPro: 1.297 ± 0.335
1.21MetGln: 1.21 ± 0.331
1.643MetArg: 1.643 ± 0.265
1.643MetSer: 1.643 ± 0.478
1.816MetThr: 1.816 ± 0.383
2.939MetVal: 2.939 ± 0.472
0.086MetTrp: 0.086 ± 0.08
0.605MetTyr: 0.605 ± 0.242
0.0MetXaa: 0.0 ± 0.0
Asn
3.285AsnAla: 3.285 ± 0.447
0.778AsnCys: 0.778 ± 0.242
2.68AsnAsp: 2.68 ± 0.406
2.334AsnGlu: 2.334 ± 0.462
1.643AsnPhe: 1.643 ± 0.383
4.409AsnGly: 4.409 ± 0.615
0.778AsnHis: 0.778 ± 0.276
2.68AsnIle: 2.68 ± 0.406
2.68AsnLys: 2.68 ± 0.418
3.372AsnLeu: 3.372 ± 0.551
0.951AsnMet: 0.951 ± 0.367
1.556AsnAsn: 1.556 ± 0.347
2.68AsnPro: 2.68 ± 0.481
1.47AsnGln: 1.47 ± 0.329
2.421AsnArg: 2.421 ± 0.604
2.594AsnSer: 2.594 ± 0.693
2.334AsnThr: 2.334 ± 0.632
2.939AsnVal: 2.939 ± 0.446
0.432AsnTrp: 0.432 ± 0.153
1.643AsnTyr: 1.643 ± 0.339
0.0AsnXaa: 0.0 ± 0.0
Pro
2.853ProAla: 2.853 ± 0.402
0.432ProCys: 0.432 ± 0.22
2.594ProAsp: 2.594 ± 0.486
4.409ProGlu: 4.409 ± 0.825
1.297ProPhe: 1.297 ± 0.307
0.519ProGly: 0.519 ± 0.211
0.692ProHis: 0.692 ± 0.229
1.556ProIle: 1.556 ± 0.337
2.594ProLys: 2.594 ± 0.466
2.248ProLeu: 2.248 ± 0.469
1.297ProMet: 1.297 ± 0.335
2.594ProAsn: 2.594 ± 0.592
0.605ProPro: 0.605 ± 0.206
1.037ProGln: 1.037 ± 0.301
1.47ProArg: 1.47 ± 0.379
2.334ProSer: 2.334 ± 0.392
1.902ProThr: 1.902 ± 0.446
1.988ProVal: 1.988 ± 0.349
0.692ProTrp: 0.692 ± 0.204
1.21ProTyr: 1.21 ± 0.321
0.0ProXaa: 0.0 ± 0.0
Gln
3.717GlnAla: 3.717 ± 0.958
0.173GlnCys: 0.173 ± 0.13
1.988GlnAsp: 1.988 ± 0.384
3.112GlnGlu: 3.112 ± 0.521
1.988GlnPhe: 1.988 ± 0.386
2.161GlnGly: 2.161 ± 0.453
0.346GlnHis: 0.346 ± 0.188
1.383GlnIle: 1.383 ± 0.319
2.075GlnLys: 2.075 ± 0.387
3.977GlnLeu: 3.977 ± 0.542
1.383GlnMet: 1.383 ± 0.316
1.21GlnAsn: 1.21 ± 0.38
1.47GlnPro: 1.47 ± 0.42
1.643GlnGln: 1.643 ± 0.304
2.248GlnArg: 2.248 ± 0.559
1.816GlnSer: 1.816 ± 0.414
1.383GlnThr: 1.383 ± 0.387
2.421GlnVal: 2.421 ± 0.56
1.297GlnTrp: 1.297 ± 0.343
1.037GlnTyr: 1.037 ± 0.373
0.0GlnXaa: 0.0 ± 0.0
Arg
5.879ArgAla: 5.879 ± 0.772
0.951ArgCys: 0.951 ± 0.299
3.89ArgAsp: 3.89 ± 0.639
4.496ArgGlu: 4.496 ± 0.734
2.248ArgPhe: 2.248 ± 0.508
3.112ArgGly: 3.112 ± 0.461
0.865ArgHis: 0.865 ± 0.313
2.939ArgIle: 2.939 ± 0.465
4.236ArgLys: 4.236 ± 0.681
4.15ArgLeu: 4.15 ± 0.511
1.988ArgMet: 1.988 ± 0.466
2.161ArgAsn: 2.161 ± 0.49
1.902ArgPro: 1.902 ± 0.322
1.902ArgGln: 1.902 ± 0.367
2.853ArgArg: 2.853 ± 0.442
4.063ArgSer: 4.063 ± 0.633
2.68ArgThr: 2.68 ± 0.385
3.112ArgVal: 3.112 ± 0.531
0.951ArgTrp: 0.951 ± 0.241
1.902ArgTyr: 1.902 ± 0.46
0.0ArgXaa: 0.0 ± 0.0
Ser
5.014SerAla: 5.014 ± 0.681
0.951SerCys: 0.951 ± 0.365
5.187SerAsp: 5.187 ± 0.638
3.545SerGlu: 3.545 ± 0.431
2.334SerPhe: 2.334 ± 0.378
5.014SerGly: 5.014 ± 0.798
1.21SerHis: 1.21 ± 0.298
3.285SerIle: 3.285 ± 0.559
3.804SerLys: 3.804 ± 0.58
4.15SerLeu: 4.15 ± 0.537
1.556SerMet: 1.556 ± 0.427
3.026SerAsn: 3.026 ± 0.44
1.816SerPro: 1.816 ± 0.407
2.421SerGln: 2.421 ± 0.623
3.285SerArg: 3.285 ± 0.573
3.285SerSer: 3.285 ± 0.571
3.285SerThr: 3.285 ± 0.575
4.582SerVal: 4.582 ± 0.602
0.692SerTrp: 0.692 ± 0.156
2.248SerTyr: 2.248 ± 0.562
0.0SerXaa: 0.0 ± 0.0
Thr
4.841ThrAla: 4.841 ± 0.612
0.692ThrCys: 0.692 ± 0.3
3.199ThrAsp: 3.199 ± 0.465
3.112ThrGlu: 3.112 ± 0.581
1.816ThrPhe: 1.816 ± 0.388
5.965ThrGly: 5.965 ± 0.67
0.865ThrHis: 0.865 ± 0.239
3.199ThrIle: 3.199 ± 0.497
5.014ThrLys: 5.014 ± 0.499
3.804ThrLeu: 3.804 ± 0.586
1.21ThrMet: 1.21 ± 0.353
1.729ThrAsn: 1.729 ± 0.363
2.853ThrPro: 2.853 ± 0.465
1.729ThrGln: 1.729 ± 0.323
2.594ThrArg: 2.594 ± 0.477
3.372ThrSer: 3.372 ± 0.577
2.766ThrThr: 2.766 ± 0.658
3.199ThrVal: 3.199 ± 0.533
0.692ThrTrp: 0.692 ± 0.269
1.383ThrTyr: 1.383 ± 0.332
0.0ThrXaa: 0.0 ± 0.0
Val
5.792ValAla: 5.792 ± 0.668
0.692ValCys: 0.692 ± 0.272
3.026ValAsp: 3.026 ± 0.401
4.668ValGlu: 4.668 ± 0.805
2.334ValPhe: 2.334 ± 0.552
4.496ValGly: 4.496 ± 0.511
1.297ValHis: 1.297 ± 0.32
3.717ValIle: 3.717 ± 0.56
3.804ValLys: 3.804 ± 0.679
3.89ValLeu: 3.89 ± 0.51
1.816ValMet: 1.816 ± 0.378
3.372ValAsn: 3.372 ± 0.591
2.507ValPro: 2.507 ± 0.509
2.421ValGln: 2.421 ± 0.462
4.063ValArg: 4.063 ± 0.512
3.717ValSer: 3.717 ± 0.506
4.668ValThr: 4.668 ± 0.636
4.582ValVal: 4.582 ± 0.684
0.951ValTrp: 0.951 ± 0.215
2.334ValTyr: 2.334 ± 0.53
0.0ValXaa: 0.0 ± 0.0
Trp
0.778TrpAla: 0.778 ± 0.286
0.346TrpCys: 0.346 ± 0.17
0.519TrpAsp: 0.519 ± 0.186
1.47TrpGlu: 1.47 ± 0.277
0.519TrpPhe: 0.519 ± 0.218
0.778TrpGly: 0.778 ± 0.272
0.346TrpHis: 0.346 ± 0.178
0.865TrpIle: 0.865 ± 0.322
1.297TrpLys: 1.297 ± 0.352
1.556TrpLeu: 1.556 ± 0.388
0.346TrpMet: 0.346 ± 0.151
1.297TrpAsn: 1.297 ± 0.342
0.259TrpPro: 0.259 ± 0.132
0.432TrpGln: 0.432 ± 0.205
0.692TrpArg: 0.692 ± 0.212
1.124TrpSer: 1.124 ± 0.365
1.21TrpThr: 1.21 ± 0.302
1.729TrpVal: 1.729 ± 0.482
0.605TrpTrp: 0.605 ± 0.185
0.259TrpTyr: 0.259 ± 0.131
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.372TyrAla: 3.372 ± 0.576
0.346TyrCys: 0.346 ± 0.189
2.507TyrAsp: 2.507 ± 0.449
2.507TyrGlu: 2.507 ± 0.484
0.778TyrPhe: 0.778 ± 0.295
2.68TyrGly: 2.68 ± 0.407
0.519TyrHis: 0.519 ± 0.232
1.816TyrIle: 1.816 ± 0.463
1.729TyrLys: 1.729 ± 0.349
2.766TyrLeu: 2.766 ± 0.445
0.951TyrMet: 0.951 ± 0.296
1.902TyrAsn: 1.902 ± 0.299
0.865TyrPro: 0.865 ± 0.28
1.124TyrGln: 1.124 ± 0.391
2.075TyrArg: 2.075 ± 0.468
2.248TyrSer: 2.248 ± 0.557
1.988TyrThr: 1.988 ± 0.396
2.594TyrVal: 2.594 ± 0.502
0.432TyrTrp: 0.432 ± 0.178
0.865TyrTyr: 0.865 ± 0.279
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 49 proteins (11568 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski