Amino acid dipepetide frequency for Bacillus pumilus (strain SAFR-032)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.701AlaAla: 6.701 ± 0.105	0.674AlaCys: 0.674 ± 0.027	3.688AlaAsp: 3.688 ± 0.06	4.708AlaGlu: 4.708 ± 0.076	3.681AlaPhe: 3.681 ± 0.07	5.538AlaGly: 5.538 ± 0.088	1.645AlaHis: 1.645 ± 0.041	5.704AlaIle: 5.704 ± 0.085	5.141AlaLys: 5.141 ± 0.081	7.713AlaLeu: 7.713 ± 0.106	2.095AlaMet: 2.095 ± 0.054	2.557AlaAsn: 2.557 ± 0.052	2.166AlaPro: 2.166 ± 0.046	2.434AlaGln: 2.434 ± 0.054	2.594AlaArg: 2.594 ± 0.05	4.611AlaSer: 4.611 ± 0.064	3.322AlaThr: 3.322 ± 0.156	5.805AlaVal: 5.805 ± 0.094	0.556AlaTrp: 0.556 ± 0.026	2.488AlaTyr: 2.488 ± 0.058	0.0AlaXaa: 0.0 ± 0.0
Cys	0.505CysAla: 0.505 ± 0.023	0.093CysCys: 0.093 ± 0.01	0.368CysAsp: 0.368 ± 0.018	0.439CysGlu: 0.439 ± 0.02	0.339CysPhe: 0.339 ± 0.017	0.651CysGly: 0.651 ± 0.025	0.257CysHis: 0.257 ± 0.016	0.522CysIle: 0.522 ± 0.021	0.299CysLys: 0.299 ± 0.016	0.813CysLeu: 0.813 ± 0.03	0.217CysMet: 0.217 ± 0.014	0.194CysAsn: 0.194 ± 0.014	0.335CysPro: 0.335 ± 0.018	0.298CysGln: 0.298 ± 0.016	0.27CysArg: 0.27 ± 0.018	0.451CysSer: 0.451 ± 0.018	0.378CysThr: 0.378 ± 0.021	0.452CysVal: 0.452 ± 0.021	0.069CysTrp: 0.069 ± 0.007	0.241CysTyr: 0.241 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.591AspAla: 3.591 ± 0.067	0.348AspCys: 0.348 ± 0.019	2.708AspAsp: 2.708 ± 0.062	4.598AspGlu: 4.598 ± 0.074	2.215AspPhe: 2.215 ± 0.042	3.411AspGly: 3.411 ± 0.061	1.563AspHis: 1.563 ± 0.039	4.193AspIle: 4.193 ± 0.078	2.745AspLys: 2.745 ± 0.054	4.957AspLeu: 4.957 ± 0.078	1.567AspMet: 1.567 ± 0.041	1.252AspAsn: 1.252 ± 0.04	2.002AspPro: 2.002 ± 0.038	3.007AspGln: 3.007 ± 0.06	2.368AspArg: 2.368 ± 0.047	2.289AspSer: 2.289 ± 0.052	2.549AspThr: 2.549 ± 0.049	3.988AspVal: 3.988 ± 0.073	0.585AspTrp: 0.585 ± 0.025	1.895AspTyr: 1.895 ± 0.05	0.0AspXaa: 0.0 ± 0.0
Glu	5.942GluAla: 5.942 ± 0.096	0.367GluCys: 0.367 ± 0.018	3.758GluAsp: 3.758 ± 0.072	6.896GluGlu: 6.896 ± 0.107	2.269GluPhe: 2.269 ± 0.05	4.193GluGly: 4.193 ± 0.061	1.827GluHis: 1.827 ± 0.048	4.513GluIle: 4.513 ± 0.07	6.761GluLys: 6.761 ± 0.085	6.96GluLeu: 6.96 ± 0.093	2.186GluMet: 2.186 ± 0.046	2.873GluAsn: 2.873 ± 0.051	2.026GluPro: 2.026 ± 0.042	3.84GluGln: 3.84 ± 0.074	3.621GluArg: 3.621 ± 0.064	3.418GluSer: 3.418 ± 0.057	4.116GluThr: 4.116 ± 0.064	4.696GluVal: 4.696 ± 0.07	0.821GluTrp: 0.821 ± 0.03	2.019GluTyr: 2.019 ± 0.044	0.0GluXaa: 0.0 ± 0.0
Phe	3.011PheAla: 3.011 ± 0.055	0.363PheCys: 0.363 ± 0.017	2.494PheAsp: 2.494 ± 0.05	2.891PheGlu: 2.891 ± 0.052	2.484PhePhe: 2.484 ± 0.058	3.217PheGly: 3.217 ± 0.068	1.285PheHis: 1.285 ± 0.037	3.717PheIle: 3.717 ± 0.077	2.383PheLys: 2.383 ± 0.05	4.49PheLeu: 4.49 ± 0.095	1.194PheMet: 1.194 ± 0.036	1.447PheAsn: 1.447 ± 0.036	1.561PhePro: 1.561 ± 0.04	2.064PheGln: 2.064 ± 0.048	1.504PheArg: 1.504 ± 0.037	3.235PheSer: 3.235 ± 0.066	2.615PheThr: 2.615 ± 0.047	3.128PheVal: 3.128 ± 0.055	0.431PheTrp: 0.431 ± 0.02	1.686PheTyr: 1.686 ± 0.047	0.0PheXaa: 0.0 ± 0.0
Gly	5.253GlyAla: 5.253 ± 0.163	0.588GlyCys: 0.588 ± 0.025	3.295GlyAsp: 3.295 ± 0.062	4.291GlyGlu: 4.291 ± 0.065	3.409GlyPhe: 3.409 ± 0.054	4.812GlyGly: 4.812 ± 0.095	1.554GlyHis: 1.554 ± 0.045	5.62GlyIle: 5.62 ± 0.124	4.896GlyLys: 4.896 ± 0.077	6.537GlyLeu: 6.537 ± 0.085	2.141GlyMet: 2.141 ± 0.056	2.208GlyAsn: 2.208 ± 0.051	1.788GlyPro: 1.788 ± 0.096	2.478GlyGln: 2.478 ± 0.056	2.676GlyArg: 2.676 ± 0.055	3.916GlySer: 3.916 ± 0.106	4.168GlyThr: 4.168 ± 0.066	4.993GlyVal: 4.993 ± 0.075	0.753GlyTrp: 0.753 ± 0.03	2.536GlyTyr: 2.536 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	1.676HisAla: 1.676 ± 0.036	0.223HisCys: 0.223 ± 0.014	1.283HisAsp: 1.283 ± 0.032	1.813HisGlu: 1.813 ± 0.042	1.266HisPhe: 1.266 ± 0.036	1.48HisGly: 1.48 ± 0.038	0.937HisHis: 0.937 ± 0.036	1.925HisIle: 1.925 ± 0.046	1.053HisLys: 1.053 ± 0.031	2.641HisLeu: 2.641 ± 0.057	0.742HisMet: 0.742 ± 0.026	0.635HisAsn: 0.635 ± 0.024	1.37HisPro: 1.37 ± 0.044	1.301HisGln: 1.301 ± 0.038	0.934HisArg: 0.934 ± 0.03	1.346HisSer: 1.346 ± 0.037	1.341HisThr: 1.341 ± 0.033	1.824HisVal: 1.824 ± 0.043	0.238HisTrp: 0.238 ± 0.018	0.966HisTyr: 0.966 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	5.802IleAla: 5.802 ± 0.09	0.687IleCys: 0.687 ± 0.026	4.279IleAsp: 4.279 ± 0.06	5.708IleGlu: 5.708 ± 0.083	3.014IlePhe: 3.014 ± 0.068	5.993IleGly: 5.993 ± 0.098	1.918IleHis: 1.918 ± 0.046	4.953IleIle: 4.953 ± 0.082	4.089IleLys: 4.089 ± 0.062	6.629IleLeu: 6.629 ± 0.092	1.674IleMet: 1.674 ± 0.042	2.426IleAsn: 2.426 ± 0.046	3.137IlePro: 3.137 ± 0.059	3.287IleGln: 3.287 ± 0.053	2.898IleArg: 2.898 ± 0.057	4.78IleSer: 4.78 ± 0.069	3.942IleThr: 3.942 ± 0.117	5.328IleVal: 5.328 ± 0.09	0.61IleTrp: 0.61 ± 0.027	2.24IleTyr: 2.24 ± 0.057	0.0IleXaa: 0.0 ± 0.0
Lys	5.279LysAla: 5.279 ± 0.07	0.277LysCys: 0.277 ± 0.017	4.06LysAsp: 4.06 ± 0.072	7.257LysGlu: 7.257 ± 0.096	1.477LysPhe: 1.477 ± 0.044	4.988LysGly: 4.988 ± 0.074	1.567LysHis: 1.567 ± 0.044	3.916LysIle: 3.916 ± 0.055	6.678LysLys: 6.678 ± 0.104	5.472LysLeu: 5.472 ± 0.085	2.211LysMet: 2.211 ± 0.045	2.85LysAsn: 2.85 ± 0.059	2.34LysPro: 2.34 ± 0.049	4.071LysGln: 4.071 ± 0.068	3.744LysArg: 3.744 ± 0.061	3.364LysSer: 3.364 ± 0.063	3.998LysThr: 3.998 ± 0.063	4.269LysVal: 4.269 ± 0.076	0.812LysTrp: 0.812 ± 0.027	1.688LysTyr: 1.688 ± 0.039	0.0LysXaa: 0.0 ± 0.0
Leu	7.217LeuAla: 7.217 ± 0.079	0.743LeuCys: 0.743 ± 0.028	4.8LeuAsp: 4.8 ± 0.075	6.148LeuGlu: 6.148 ± 0.089	5.094LeuPhe: 5.094 ± 0.093	6.024LeuGly: 6.024 ± 0.094	2.333LeuHis: 2.333 ± 0.053	7.09LeuIle: 7.09 ± 0.101	7.232LeuLys: 7.232 ± 0.088	10.142LeuLeu: 10.142 ± 0.136	2.702LeuMet: 2.702 ± 0.051	3.925LeuAsn: 3.925 ± 0.066	3.931LeuPro: 3.931 ± 0.066	3.733LeuGln: 3.733 ± 0.07	3.467LeuArg: 3.467 ± 0.067	7.299LeuSer: 7.299 ± 0.097	6.091LeuThr: 6.091 ± 0.082	6.048LeuVal: 6.048 ± 0.086	0.758LeuTrp: 0.758 ± 0.028	3.185LeuTyr: 3.185 ± 0.064	0.0LeuXaa: 0.0 ± 0.0
Met	2.028MetAla: 2.028 ± 0.046	0.168MetCys: 0.168 ± 0.013	1.437MetAsp: 1.437 ± 0.036	1.632MetGlu: 1.632 ± 0.036	1.137MetPhe: 1.137 ± 0.032	1.657MetGly: 1.657 ± 0.046	0.47MetHis: 0.47 ± 0.021	2.24MetIle: 2.24 ± 0.054	3.0MetLys: 3.0 ± 0.054	2.894MetLeu: 2.894 ± 0.057	1.021MetMet: 1.021 ± 0.035	1.657MetAsn: 1.657 ± 0.035	1.056MetPro: 1.056 ± 0.035	0.973MetGln: 0.973 ± 0.031	1.168MetArg: 1.168 ± 0.035	1.943MetSer: 1.943 ± 0.039	2.072MetThr: 2.072 ± 0.049	1.623MetVal: 1.623 ± 0.04	0.192MetTrp: 0.192 ± 0.013	0.8MetTyr: 0.8 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	2.424AsnAla: 2.424 ± 0.052	0.231AsnCys: 0.231 ± 0.017	1.953AsnAsp: 1.953 ± 0.04	3.267AsnGlu: 3.267 ± 0.053	1.191AsnPhe: 1.191 ± 0.035	3.036AsnGly: 3.036 ± 0.063	0.913AsnHis: 0.913 ± 0.034	2.75AsnIle: 2.75 ± 0.056	2.273AsnLys: 2.273 ± 0.054	2.926AsnLeu: 2.926 ± 0.053	1.105AsnMet: 1.105 ± 0.037	1.298AsnAsn: 1.298 ± 0.045	1.736AsnPro: 1.736 ± 0.043	1.966AsnGln: 1.966 ± 0.045	1.831AsnArg: 1.831 ± 0.046	1.794AsnSer: 1.794 ± 0.048	1.782AsnThr: 1.782 ± 0.037	2.592AsnVal: 2.592 ± 0.051	0.424AsnTrp: 0.424 ± 0.019	1.123AsnTyr: 1.123 ± 0.037	0.0AsnXaa: 0.0 ± 0.0
Pro	2.634ProAla: 2.634 ± 0.047	0.202ProCys: 0.202 ± 0.014	1.994ProAsp: 1.994 ± 0.05	2.786ProGlu: 2.786 ± 0.067	2.116ProPhe: 2.116 ± 0.047	2.102ProGly: 2.102 ± 0.045	1.027ProHis: 1.027 ± 0.032	2.621ProIle: 2.621 ± 0.044	2.3ProLys: 2.3 ± 0.051	3.596ProLeu: 3.596 ± 0.063	0.814ProMet: 0.814 ± 0.031	1.537ProAsn: 1.537 ± 0.041	0.974ProPro: 0.974 ± 0.037	1.181ProGln: 1.181 ± 0.035	1.046ProArg: 1.046 ± 0.038	2.398ProSer: 2.398 ± 0.043	1.812ProThr: 1.812 ± 0.096	3.066ProVal: 3.066 ± 0.052	0.322ProTrp: 0.322 ± 0.018	1.485ProTyr: 1.485 ± 0.041	0.0ProXaa: 0.0 ± 0.0
Gln	3.281GlnAla: 3.281 ± 0.056	0.203GlnCys: 0.203 ± 0.015	1.98GlnAsp: 1.98 ± 0.047	3.128GlnGlu: 3.128 ± 0.065	1.766GlnPhe: 1.766 ± 0.039	2.245GlnGly: 2.245 ± 0.04	1.26GlnHis: 1.26 ± 0.038	2.836GlnIle: 2.836 ± 0.058	3.44GlnLys: 3.44 ± 0.062	4.795GlnLeu: 4.795 ± 0.08	1.499GlnMet: 1.499 ± 0.035	1.605GlnAsn: 1.605 ± 0.037	1.489GlnPro: 1.489 ± 0.046	2.309GlnGln: 2.309 ± 0.061	1.587GlnArg: 1.587 ± 0.037	2.576GlnSer: 2.576 ± 0.059	2.78GlnThr: 2.78 ± 0.062	2.782GlnVal: 2.782 ± 0.054	0.355GlnTrp: 0.355 ± 0.02	1.449GlnTyr: 1.449 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	2.51ArgAla: 2.51 ± 0.055	0.23ArgCys: 0.23 ± 0.014	1.96ArgAsp: 1.96 ± 0.044	2.947ArgGlu: 2.947 ± 0.057	1.976ArgPhe: 1.976 ± 0.044	2.23ArgGly: 2.23 ± 0.044	1.009ArgHis: 1.009 ± 0.032	2.99ArgIle: 2.99 ± 0.055	3.054ArgLys: 3.054 ± 0.062	4.232ArgLeu: 4.232 ± 0.071	1.385ArgMet: 1.385 ± 0.038	1.515ArgAsn: 1.515 ± 0.042	1.362ArgPro: 1.362 ± 0.034	1.744ArgGln: 1.744 ± 0.042	1.761ArgArg: 1.761 ± 0.045	2.361ArgSer: 2.361 ± 0.047	2.208ArgThr: 2.208 ± 0.049	2.534ArgVal: 2.534 ± 0.05	0.378ArgTrp: 0.378 ± 0.016	1.528ArgTyr: 1.528 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	4.225SerAla: 4.225 ± 0.067	0.437SerCys: 0.437 ± 0.021	2.849SerAsp: 2.849 ± 0.064	3.73SerGlu: 3.73 ± 0.062	3.565SerPhe: 3.565 ± 0.069	4.408SerGly: 4.408 ± 0.076	1.396SerHis: 1.396 ± 0.04	4.942SerIle: 4.942 ± 0.076	3.76SerLys: 3.76 ± 0.062	6.239SerLeu: 6.239 ± 0.084	1.953SerMet: 1.953 ± 0.041	2.116SerAsn: 2.116 ± 0.047	2.141SerPro: 2.141 ± 0.046	2.271SerGln: 2.271 ± 0.047	2.166SerArg: 2.166 ± 0.045	4.21SerSer: 4.21 ± 0.086	3.222SerThr: 3.222 ± 0.101	4.161SerVal: 4.161 ± 0.069	0.556SerTrp: 0.556 ± 0.023	2.355SerTyr: 2.355 ± 0.053	0.0SerXaa: 0.0 ± 0.0
Thr	4.322ThrAla: 4.322 ± 0.068	0.4ThrCys: 0.4 ± 0.02	2.897ThrAsp: 2.897 ± 0.051	3.397ThrGlu: 3.397 ± 0.064	2.844ThrPhe: 2.844 ± 0.052	4.816ThrGly: 4.816 ± 0.453	1.295ThrHis: 1.295 ± 0.038	4.359ThrIle: 4.359 ± 0.063	3.627ThrLys: 3.627 ± 0.059	5.494ThrLeu: 5.494 ± 0.068	1.337ThrMet: 1.337 ± 0.035	2.303ThrAsn: 2.303 ± 0.048	2.325ThrPro: 2.325 ± 0.045	1.577ThrGln: 1.577 ± 0.043	1.77ThrArg: 1.77 ± 0.044	3.546ThrSer: 3.546 ± 0.056	2.842ThrThr: 2.842 ± 0.058	4.193ThrVal: 4.193 ± 0.062	0.497ThrTrp: 0.497 ± 0.021	2.129ThrTyr: 2.129 ± 0.051	0.0ThrXaa: 0.0 ± 0.0
Val	4.65ValAla: 4.65 ± 0.081	0.621ValCys: 0.621 ± 0.024	3.512ValAsp: 3.512 ± 0.06	4.255ValGlu: 4.255 ± 0.076	3.158ValPhe: 3.158 ± 0.068	4.216ValGly: 4.216 ± 0.075	1.571ValHis: 1.571 ± 0.043	5.467ValIle: 5.467 ± 0.074	5.125ValLys: 5.125 ± 0.082	7.118ValLeu: 7.118 ± 0.088	2.012ValMet: 2.012 ± 0.04	2.707ValAsn: 2.707 ± 0.053	2.65ValPro: 2.65 ± 0.054	2.699ValGln: 2.699 ± 0.052	2.603ValArg: 2.603 ± 0.054	4.767ValSer: 4.767 ± 0.074	4.293ValThr: 4.293 ± 0.072	4.886ValVal: 4.886 ± 0.085	0.629ValTrp: 0.629 ± 0.026	2.341ValTyr: 2.341 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	0.535TrpAla: 0.535 ± 0.023	0.077TrpCys: 0.077 ± 0.008	0.476TrpAsp: 0.476 ± 0.019	0.549TrpGlu: 0.549 ± 0.021	0.505TrpPhe: 0.505 ± 0.025	0.584TrpGly: 0.584 ± 0.027	0.228TrpHis: 0.228 ± 0.016	0.748TrpIle: 0.748 ± 0.029	0.683TrpLys: 0.683 ± 0.028	1.103TrpLeu: 1.103 ± 0.034	0.372TrpMet: 0.372 ± 0.017	0.458TrpAsn: 0.458 ± 0.023	0.237TrpPro: 0.237 ± 0.015	0.317TrpGln: 0.317 ± 0.018	0.36TrpArg: 0.36 ± 0.018	0.572TrpSer: 0.572 ± 0.024	0.598TrpThr: 0.598 ± 0.023	0.608TrpVal: 0.608 ± 0.025	0.123TrpTrp: 0.123 ± 0.012	0.343TrpTyr: 0.343 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.234TyrAla: 2.234 ± 0.047	0.256TyrCys: 0.256 ± 0.017	2.041TyrAsp: 2.041 ± 0.049	2.662TyrGlu: 2.662 ± 0.063	1.694TyrPhe: 1.694 ± 0.04	2.308TyrGly: 2.308 ± 0.05	0.941TyrHis: 0.941 ± 0.032	2.288TyrIle: 2.288 ± 0.046	1.775TyrLys: 1.775 ± 0.042	3.213TyrLeu: 3.213 ± 0.056	0.938TyrMet: 0.938 ± 0.027	1.054TyrAsn: 1.054 ± 0.036	1.389TyrPro: 1.389 ± 0.036	1.814TyrGln: 1.814 ± 0.045	1.548TyrArg: 1.548 ± 0.037	1.826TyrSer: 1.826 ± 0.044	1.842TyrThr: 1.842 ± 0.046	2.303TyrVal: 2.303 ± 0.051	0.368TyrTrp: 0.368 ± 0.02	1.282TyrTyr: 1.282 ± 0.038	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3700 proteins (1071977 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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