Ovis aries (Sheep)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria;

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23135 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W5QBC1|W5QBC1_SHEEP CDP-diacylglycerol synthase OS=Ovis aries OX=9940 GN=TAMM41 PE=3 SV=1
VV1 pKa = 7.35GLRR4 pKa = 11.84QLDD7 pKa = 3.73MSLLCQLYY15 pKa = 10.36SLYY18 pKa = 10.95EE19 pKa = 4.19SIQEE23 pKa = 4.04YY24 pKa = 10.64KK25 pKa = 10.7GACQAAASPDD35 pKa = 3.24STYY38 pKa = 11.63ALEE41 pKa = 4.9NGFFDD46 pKa = 4.92EE47 pKa = 4.81DD48 pKa = 3.59DD49 pKa = 4.62EE50 pKa = 4.95YY51 pKa = 11.39FQEE54 pKa = 4.61QNSLQDD60 pKa = 3.44GKK62 pKa = 11.19EE63 pKa = 3.79RR64 pKa = 11.84GPPRR68 pKa = 11.84DD69 pKa = 3.15LTLPVSPLPSGDD81 pKa = 3.18WVLDD85 pKa = 3.68SMM87 pKa = 5.67

Molecular weight: 9.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W5Q529|W5Q529_SHEEP Uncharacterized protein OS=Ovis aries OX=9940 GN=CDRT4 PE=4 SV=1
RR1 pKa = 7.48GFRR4 pKa = 11.84RR5 pKa = 11.84QPPAAPRR12 pKa = 11.84SPPGAVSGKK21 pKa = 10.13KK22 pKa = 9.92SGGTRR27 pKa = 11.84RR28 pKa = 11.84PGRR31 pKa = 11.84GRR33 pKa = 11.84GRR35 pKa = 11.84RR36 pKa = 11.84GGRR39 pKa = 11.84RR40 pKa = 11.84GGGGQRR46 pKa = 11.84QGAPGPRR53 pKa = 11.84APP55 pKa = 4.76

Molecular weight: 5.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21220

1915

23135

12233952

3

35229

528.8

58.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.93 ± 0.017

2.252 ± 0.012

4.759 ± 0.011

7.018 ± 0.022

3.715 ± 0.01

6.548 ± 0.026

2.56 ± 0.009

4.395 ± 0.016

5.735 ± 0.026

10.044 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.095 ± 0.007

3.557 ± 0.012

6.308 ± 0.027

4.702 ± 0.017

5.672 ± 0.017

8.309 ± 0.021

5.313 ± 0.014

6.12 ± 0.016

1.249 ± 0.005

2.659 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski