Wenzhou yanvirus-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KL64|A0A1L3KL64_9VIRU RNA-dependent RNA polymerase OS=Wenzhou yanvirus-like virus 1 OX=1923683 PE=4 SV=1
MM1 pKa = 7.71VDD3 pKa = 2.92QCRR6 pKa = 11.84FLGLSDD12 pKa = 3.53FPIRR16 pKa = 11.84VMGPVGTVSHH26 pKa = 5.74YY27 pKa = 10.8VCGKK31 pKa = 8.86PRR33 pKa = 11.84IDD35 pKa = 3.98GACLLYY41 pKa = 10.44IPARR45 pKa = 11.84PPLVPLAHH53 pKa = 6.0WTIAVGGQSYY63 pKa = 10.87DD64 pKa = 3.69YY65 pKa = 10.93ADD67 pKa = 3.97PAALGGVVLYY77 pKa = 11.07SDD79 pKa = 4.98IPPISYY85 pKa = 9.64NPHH88 pKa = 5.18VFWHH92 pKa = 6.51AGSCKK97 pKa = 10.23SATLQFWMRR106 pKa = 11.84AGNGCRR112 pKa = 11.84CKK114 pKa = 10.4RR115 pKa = 11.84FCRR118 pKa = 11.84HH119 pKa = 4.35QFMWEE124 pKa = 4.12YY125 pKa = 10.84NHH127 pKa = 6.54EE128 pKa = 4.1LANRR132 pKa = 11.84GLPAGTGPVAFMLSCRR148 pKa = 11.84IHH150 pKa = 6.19PRR152 pKa = 11.84KK153 pKa = 9.93LYY155 pKa = 10.3NPRR158 pKa = 11.84TVRR161 pKa = 11.84FGSGRR166 pKa = 11.84PGQYY170 pKa = 8.51VAHH173 pKa = 7.75ILRR176 pKa = 11.84CPYY179 pKa = 10.05SDD181 pKa = 3.55YY182 pKa = 11.36DD183 pKa = 3.82VRR185 pKa = 11.84ISNCTTVQIVTSRR198 pKa = 11.84GIVYY202 pKa = 9.5SQNKK206 pKa = 6.48ATSMEE211 pKa = 4.46FLNKK215 pKa = 8.6THH217 pKa = 6.85LCVPTGEE224 pKa = 3.95FMQWGGVALEE234 pKa = 4.41LLRR237 pKa = 11.84IEE239 pKa = 4.29PQPVISFTPSPRR251 pKa = 11.84TRR253 pKa = 11.84GRR255 pKa = 11.84FYY257 pKa = 11.21LVVGVAKK264 pKa = 10.21AVSSMFSRR272 pKa = 11.84NPLVRR277 pKa = 11.84YY278 pKa = 8.61HH279 pKa = 3.92VTYY282 pKa = 10.69RR283 pKa = 11.84RR284 pKa = 11.84FASASTFCAIGVAGALMFEE303 pKa = 4.64LRR305 pKa = 11.84SIARR309 pKa = 11.84SLFNMRR315 pKa = 11.84SQQRR319 pKa = 11.84FFPMQVEE326 pKa = 4.15YY327 pKa = 10.97DD328 pKa = 3.72VATFAPYY335 pKa = 10.38NIQLPFEE342 pKa = 4.28QEE344 pKa = 3.47LFMRR348 pKa = 11.84MAVKK352 pKa = 10.31RR353 pKa = 11.84DD354 pKa = 3.15ITDD357 pKa = 2.4AWIRR361 pKa = 11.84DD362 pKa = 3.88VITRR366 pKa = 11.84LAHH369 pKa = 5.15QNDD372 pKa = 3.11WRR374 pKa = 11.84GVLNRR379 pKa = 11.84HH380 pKa = 6.27EE381 pKa = 4.36LNQWISRR388 pKa = 11.84VVTEE392 pKa = 5.21PGLVWEE398 pKa = 4.41VQKK401 pKa = 11.39DD402 pKa = 3.78GLRR405 pKa = 11.84NNACYY410 pKa = 9.45TCLSIGRR417 pKa = 11.84KK418 pKa = 6.85MRR420 pKa = 11.84RR421 pKa = 11.84NEE423 pKa = 3.82CAQCRR428 pKa = 11.84TQRR431 pKa = 11.84NLSPLYY437 pKa = 7.53FTPIPYY443 pKa = 9.27TPFAHH448 pKa = 6.11VGLVGIYY455 pKa = 9.84SRR457 pKa = 11.84PPLLPPATYY466 pKa = 10.23YY467 pKa = 10.53HH468 pKa = 6.24SCEE471 pKa = 4.06EE472 pKa = 4.08RR473 pKa = 11.84YY474 pKa = 10.11LPYY477 pKa = 10.44KK478 pKa = 10.22SGGWYY483 pKa = 9.29PGRR486 pKa = 11.84PFEE489 pKa = 5.16FIYY492 pKa = 10.48HH493 pKa = 6.59CSYY496 pKa = 10.15KK497 pKa = 10.82GKK499 pKa = 10.11IVNDD503 pKa = 3.87PEE505 pKa = 3.97TAYY508 pKa = 10.45AIYY511 pKa = 10.69NSFGPQPRR519 pKa = 11.84CRR521 pKa = 11.84GKK523 pKa = 10.81LCGPMLAGAHH533 pKa = 5.17MQCFTSGTEE542 pKa = 3.83TALLAFVGRR551 pKa = 11.84MGAVSPEE558 pKa = 3.92YY559 pKa = 10.34QHH561 pKa = 6.42YY562 pKa = 10.78LSLGVNHH569 pKa = 6.35SVYY572 pKa = 11.23NDD574 pKa = 3.16IAKK577 pKa = 10.47VNARR581 pKa = 11.84HH582 pKa = 6.02KK583 pKa = 10.55FDD585 pKa = 3.73YY586 pKa = 10.74LLEE589 pKa = 4.34IFKK592 pKa = 10.86HH593 pKa = 5.27VGAAAYY599 pKa = 10.72YY600 pKa = 10.82DD601 pKa = 3.87GDD603 pKa = 3.77NDD605 pKa = 4.71AGGLRR610 pKa = 11.84SKK612 pKa = 10.1YY613 pKa = 8.72IPSRR617 pKa = 11.84EE618 pKa = 4.07VVPWTPQQVIDD629 pKa = 4.54HH630 pKa = 6.31QPDD633 pKa = 3.33SAKK636 pKa = 10.17RR637 pKa = 11.84RR638 pKa = 11.84IMIEE642 pKa = 3.83AYY644 pKa = 10.21RR645 pKa = 11.84DD646 pKa = 3.15IDD648 pKa = 3.92EE649 pKa = 5.48GFLTPTPKK657 pKa = 10.4LYY659 pKa = 10.06KK660 pKa = 10.34AKK662 pKa = 10.57AIAKK666 pKa = 9.26GEE668 pKa = 3.82KK669 pKa = 10.09SMAWDD674 pKa = 3.5HH675 pKa = 7.15DD676 pKa = 3.58ISEE679 pKa = 4.27TLIYY683 pKa = 10.17KK684 pKa = 10.02SKK686 pKa = 10.2QVPRR690 pKa = 11.84FISACSPHH698 pKa = 6.34TNALIAPYY706 pKa = 9.57IAAAEE711 pKa = 4.12EE712 pKa = 4.37FVKK715 pKa = 10.22EE716 pKa = 4.36FCSSSASVAYY726 pKa = 10.09ASGMNPEE733 pKa = 4.91QLNEE737 pKa = 3.96WLNTYY742 pKa = 10.55AVDD745 pKa = 3.74RR746 pKa = 11.84LIIEE750 pKa = 4.61GDD752 pKa = 3.12ISSADD757 pKa = 3.39SAIGVEE763 pKa = 3.79AHH765 pKa = 6.31YY766 pKa = 11.04LFFYY770 pKa = 10.6FLTWLFPEE778 pKa = 4.95LPEE781 pKa = 4.97SDD783 pKa = 3.37VWHH786 pKa = 6.63VIQALSYY793 pKa = 10.4IYY795 pKa = 10.66VDD797 pKa = 3.27RR798 pKa = 11.84DD799 pKa = 3.03GSKK802 pKa = 10.49FVAGPVNVSGSRR814 pKa = 11.84YY815 pKa = 6.98TSCIVTIWVMVANVTSSAFSCLSDD839 pKa = 3.83YY840 pKa = 9.25RR841 pKa = 11.84TLSQADD847 pKa = 4.29RR848 pKa = 11.84IGMVKK853 pKa = 10.5GLLQSGNAATITCSDD868 pKa = 4.69DD869 pKa = 3.0IYY871 pKa = 10.68TAVNTSFSEE880 pKa = 3.75WHH882 pKa = 5.7FMRR885 pKa = 11.84NISPYY890 pKa = 9.89LRR892 pKa = 11.84AYY894 pKa = 9.37TYY896 pKa = 9.92EE897 pKa = 4.42CSVDD901 pKa = 3.93SAWLRR906 pKa = 11.84RR907 pKa = 11.84FCDD910 pKa = 3.28TMRR913 pKa = 11.84TEE915 pKa = 4.37FFLDD919 pKa = 3.26VRR921 pKa = 11.84PNKK924 pKa = 9.89CRR926 pKa = 11.84LFPAAEE932 pKa = 3.64WRR934 pKa = 11.84LATFLAMRR942 pKa = 11.84PVWSGSRR949 pKa = 11.84YY950 pKa = 7.5EE951 pKa = 4.34WGPEE955 pKa = 2.86ICRR958 pKa = 11.84RR959 pKa = 11.84MRR961 pKa = 11.84SAYY964 pKa = 8.71WMFDD968 pKa = 3.15KK969 pKa = 10.48EE970 pKa = 4.7HH971 pKa = 7.14PPMAWGRR978 pKa = 11.84GISAALLVAGRR989 pKa = 11.84HH990 pKa = 4.53VPVLRR995 pKa = 11.84DD996 pKa = 2.77ICTWHH1001 pKa = 6.98LSVTHH1006 pKa = 6.07GPIMKK1011 pKa = 10.36LPFTNIWSTFYY1022 pKa = 10.32RR1023 pKa = 11.84YY1024 pKa = 9.01EE1025 pKa = 3.91VSGEE1029 pKa = 3.89FTDD1032 pKa = 4.31RR1033 pKa = 11.84GLNEE1037 pKa = 3.96FLADD1041 pKa = 3.8YY1042 pKa = 10.36DD1043 pKa = 3.82ISTSEE1048 pKa = 4.0YY1049 pKa = 10.66LDD1051 pKa = 4.09FVDD1054 pKa = 5.38KK1055 pKa = 11.3LKK1057 pKa = 11.38GCMDD1061 pKa = 4.27PFINIQHH1068 pKa = 5.86SVIQKK1073 pKa = 10.15LLMRR1077 pKa = 11.84EE1078 pKa = 4.05

Molecular weight:
122.67 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KL64|A0A1L3KL64_9VIRU RNA-dependent RNA polymerase OS=Wenzhou yanvirus-like virus 1 OX=1923683 PE=4 SV=1
MM1 pKa = 6.33QQKK4 pKa = 9.0TSILPSTHH12 pKa = 6.65PVRR15 pKa = 11.84SLAGLARR22 pKa = 11.84QIALPHH28 pKa = 6.06EE29 pKa = 4.76FAPEE33 pKa = 4.18RR34 pKa = 11.84FPSFPALEE42 pKa = 4.15RR43 pKa = 11.84TAVMGFNVPSTWDD56 pKa = 3.48LPAASTVKK64 pKa = 10.78AILTRR69 pKa = 11.84QATFPFWFEE78 pKa = 3.94KK79 pKa = 9.28STSGATFRR87 pKa = 11.84VTWRR91 pKa = 11.84FPRR94 pKa = 11.84APGSNTTAYY103 pKa = 10.76DD104 pKa = 3.58PVINAAGTGNVAQTINQVGVTNGNNGGIASYY135 pKa = 9.68PILGLDD141 pKa = 4.25GMGTCPYY148 pKa = 10.09MYY150 pKa = 10.4IPDD153 pKa = 3.61NWTALMVVSLDD164 pKa = 3.62VAPATAIDD172 pKa = 3.69CTVIYY177 pKa = 9.77EE178 pKa = 4.43RR179 pKa = 11.84WNSPGQASVNSVVAGSTIAGNAGCSIPISMPSSEE213 pKa = 4.45STWIRR218 pKa = 11.84IVSVSLSTSTAIALGATNVTVVASSGTLAYY248 pKa = 9.42TPGVLAGNVQVTPAATVALLPGAYY272 pKa = 8.71PVEE275 pKa = 4.55FANSQLPWYY284 pKa = 7.41STRR287 pKa = 11.84TTAAAVLCTNVSQVLNKK304 pKa = 10.5GGTVLAGRR312 pKa = 11.84IAPQVVNPWNVSEE325 pKa = 4.3TYY327 pKa = 10.65INGLHH332 pKa = 6.3PAEE335 pKa = 4.6KK336 pKa = 10.49AFLPLEE342 pKa = 4.39TGLYY346 pKa = 9.76SYY348 pKa = 10.26CPPSTDD354 pKa = 5.79LVDD357 pKa = 4.2FWDD360 pKa = 3.8YY361 pKa = 10.69TLNSTPFFTLPATPLYY377 pKa = 10.68RR378 pKa = 11.84LDD380 pKa = 3.46NTSLVNVMFVTASAVAEE397 pKa = 4.11TLAVNVDD404 pKa = 2.69WHH406 pKa = 7.65IEE408 pKa = 3.85FRR410 pKa = 11.84TSSSLFPIGLSAVTLEE426 pKa = 4.51TLHH429 pKa = 5.81QAQLALVSAGFFFEE443 pKa = 4.42NPEE446 pKa = 4.16HH447 pKa = 6.84KK448 pKa = 10.32SVLSKK453 pKa = 11.45VMTAVKK459 pKa = 10.12RR460 pKa = 11.84YY461 pKa = 9.92GPTALGAVNPAAGRR475 pKa = 11.84VARR478 pKa = 11.84TMIQLSSKK486 pKa = 9.2PASRR490 pKa = 11.84MKK492 pKa = 9.27PTSASASGFNGNKK505 pKa = 9.6KK506 pKa = 9.89PPRR509 pKa = 11.84PTPKK513 pKa = 9.87KK514 pKa = 10.61GKK516 pKa = 8.61ATSGKK521 pKa = 9.64KK522 pKa = 9.85KK523 pKa = 10.29KK524 pKa = 10.41

Molecular weight:
55.44 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1602

524

1078

801.0

89.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.176 ± 1.481

2.31 ± 0.756

3.62 ± 0.848

3.87 ± 0.562

4.744 ± 0.411

6.492 ± 0.211

2.31 ± 0.649

5.056 ± 0.585

3.371 ± 0.142

7.553 ± 0.151

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.559 ± 0.363

4.057 ± 0.717

6.866 ± 0.747

2.871 ± 0.111

6.117 ± 1.389

8.052 ± 0.724

6.617 ± 1.95

7.615 ± 0.755

2.185 ± 0.154

4.557 ± 1.051

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski