Amino acid dipepetide frequency for Gordonia phage Jumbo

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.333AlaAla: 13.333 ± 3.258
0.621AlaCys: 0.621 ± 0.171
5.59AlaAsp: 5.59 ± 0.463
5.963AlaGlu: 5.963 ± 0.62
2.567AlaPhe: 2.567 ± 0.358
8.157AlaGly: 8.157 ± 1.28
1.284AlaHis: 1.284 ± 0.243
5.217AlaIle: 5.217 ± 0.564
4.265AlaLys: 4.265 ± 0.556
6.584AlaLeu: 6.584 ± 0.632
2.526AlaMet: 2.526 ± 0.442
3.561AlaAsn: 3.561 ± 0.347
5.052AlaPro: 5.052 ± 0.741
4.141AlaGln: 4.141 ± 0.735
5.052AlaArg: 5.052 ± 0.546
4.928AlaSer: 4.928 ± 0.476
5.342AlaThr: 5.342 ± 0.59
6.501AlaVal: 6.501 ± 0.666
1.118AlaTrp: 1.118 ± 0.23
1.863AlaTyr: 1.863 ± 0.279
0.0AlaXaa: 0.0 ± 0.0
Cys
1.077CysAla: 1.077 ± 0.279
0.166CysCys: 0.166 ± 0.093
0.745CysAsp: 0.745 ± 0.208
0.497CysGlu: 0.497 ± 0.148
0.497CysPhe: 0.497 ± 0.158
0.704CysGly: 0.704 ± 0.184
0.207CysHis: 0.207 ± 0.09
0.497CysIle: 0.497 ± 0.159
0.621CysLys: 0.621 ± 0.193
0.538CysLeu: 0.538 ± 0.163
0.124CysMet: 0.124 ± 0.061
0.207CysAsn: 0.207 ± 0.098
0.745CysPro: 0.745 ± 0.209
0.166CysGln: 0.166 ± 0.081
0.455CysArg: 0.455 ± 0.127
0.621CysSer: 0.621 ± 0.16
0.455CysThr: 0.455 ± 0.149
0.497CysVal: 0.497 ± 0.167
0.166CysTrp: 0.166 ± 0.096
0.29CysTyr: 0.29 ± 0.137
0.0CysXaa: 0.0 ± 0.0
Asp
5.673AspAla: 5.673 ± 0.459
0.745AspCys: 0.745 ± 0.181
3.768AspAsp: 3.768 ± 0.593
3.644AspGlu: 3.644 ± 0.484
2.899AspPhe: 2.899 ± 0.409
4.555AspGly: 4.555 ± 0.542
1.242AspHis: 1.242 ± 0.262
4.513AspIle: 4.513 ± 0.395
2.857AspLys: 2.857 ± 0.333
4.306AspLeu: 4.306 ± 0.333
1.615AspMet: 1.615 ± 0.235
2.981AspAsn: 2.981 ± 0.574
4.596AspPro: 4.596 ± 0.5
2.195AspGln: 2.195 ± 0.319
3.354AspArg: 3.354 ± 0.491
4.679AspSer: 4.679 ± 0.593
3.354AspThr: 3.354 ± 0.375
2.981AspVal: 2.981 ± 0.289
1.159AspTrp: 1.159 ± 0.206
2.65AspTyr: 2.65 ± 0.433
0.0AspXaa: 0.0 ± 0.0
Glu
4.555GluAla: 4.555 ± 0.465
0.538GluCys: 0.538 ± 0.163
3.851GluAsp: 3.851 ± 0.481
4.431GluGlu: 4.431 ± 0.602
2.609GluPhe: 2.609 ± 0.425
3.147GluGly: 3.147 ± 0.55
1.242GluHis: 1.242 ± 0.214
5.3GluIle: 5.3 ± 0.728
4.265GluLys: 4.265 ± 0.46
5.01GluLeu: 5.01 ± 0.54
2.07GluMet: 2.07 ± 0.283
3.395GluAsn: 3.395 ± 0.458
2.981GluPro: 2.981 ± 0.511
2.236GluGln: 2.236 ± 0.28
2.981GluArg: 2.981 ± 0.415
3.892GluSer: 3.892 ± 0.499
4.141GluThr: 4.141 ± 0.367
2.981GluVal: 2.981 ± 0.284
1.035GluTrp: 1.035 ± 0.184
1.988GluTyr: 1.988 ± 0.314
0.0GluXaa: 0.0 ± 0.0
Phe
2.774PheAla: 2.774 ± 0.414
0.455PheCys: 0.455 ± 0.143
2.94PheAsp: 2.94 ± 0.379
2.774PheGlu: 2.774 ± 0.364
1.615PhePhe: 1.615 ± 0.281
3.188PheGly: 3.188 ± 0.309
0.538PheHis: 0.538 ± 0.158
2.195PheIle: 2.195 ± 0.282
1.905PheLys: 1.905 ± 0.281
2.402PheLeu: 2.402 ± 0.423
1.118PheMet: 1.118 ± 0.224
1.739PheAsn: 1.739 ± 0.212
1.698PhePro: 1.698 ± 0.292
1.449PheGln: 1.449 ± 0.214
2.153PheArg: 2.153 ± 0.271
2.774PheSer: 2.774 ± 0.405
2.236PheThr: 2.236 ± 0.231
2.153PheVal: 2.153 ± 0.281
0.745PheTrp: 0.745 ± 0.179
0.994PheTyr: 0.994 ± 0.263
0.0PheXaa: 0.0 ± 0.0
Gly
6.667GlyAla: 6.667 ± 0.869
0.331GlyCys: 0.331 ± 0.114
3.975GlyAsp: 3.975 ± 0.749
4.72GlyGlu: 4.72 ± 0.529
2.857GlyPhe: 2.857 ± 0.4
7.66GlyGly: 7.66 ± 1.649
1.573GlyHis: 1.573 ± 0.271
5.383GlyIle: 5.383 ± 0.57
4.886GlyLys: 4.886 ± 0.5
5.839GlyLeu: 5.839 ± 0.57
2.319GlyMet: 2.319 ± 0.341
4.638GlyAsn: 4.638 ± 0.635
3.23GlyPro: 3.23 ± 0.344
2.567GlyGln: 2.567 ± 0.296
3.975GlyArg: 3.975 ± 0.389
5.59GlySer: 5.59 ± 0.796
5.259GlyThr: 5.259 ± 0.477
5.756GlyVal: 5.756 ± 0.459
1.325GlyTrp: 1.325 ± 0.259
3.023GlyTyr: 3.023 ± 0.368
0.0GlyXaa: 0.0 ± 0.0
His
1.201HisAla: 1.201 ± 0.218
0.373HisCys: 0.373 ± 0.153
1.366HisAsp: 1.366 ± 0.335
0.911HisGlu: 0.911 ± 0.223
0.704HisPhe: 0.704 ± 0.193
1.532HisGly: 1.532 ± 0.277
0.58HisHis: 0.58 ± 0.16
0.538HisIle: 0.538 ± 0.154
0.952HisLys: 0.952 ± 0.245
1.242HisLeu: 1.242 ± 0.285
0.455HisMet: 0.455 ± 0.139
0.952HisAsn: 0.952 ± 0.242
1.035HisPro: 1.035 ± 0.224
0.745HisGln: 0.745 ± 0.219
1.035HisArg: 1.035 ± 0.21
1.573HisSer: 1.573 ± 0.325
1.449HisThr: 1.449 ± 0.268
1.408HisVal: 1.408 ± 0.242
0.29HisTrp: 0.29 ± 0.106
0.58HisTyr: 0.58 ± 0.165
0.0HisXaa: 0.0 ± 0.0
Ile
5.342IleAla: 5.342 ± 0.533
0.911IleCys: 0.911 ± 0.226
3.934IleAsp: 3.934 ± 0.385
4.224IleGlu: 4.224 ± 0.408
1.863IlePhe: 1.863 ± 0.279
3.437IleGly: 3.437 ± 0.508
1.201IleHis: 1.201 ± 0.233
3.602IleIle: 3.602 ± 0.378
3.23IleLys: 3.23 ± 0.454
4.845IleLeu: 4.845 ± 0.456
0.994IleMet: 0.994 ± 0.175
2.526IleAsn: 2.526 ± 0.273
3.106IlePro: 3.106 ± 0.303
2.319IleGln: 2.319 ± 0.307
2.816IleArg: 2.816 ± 0.324
3.851IleSer: 3.851 ± 0.466
3.892IleThr: 3.892 ± 0.375
3.561IleVal: 3.561 ± 0.333
1.077IleTrp: 1.077 ± 0.21
2.112IleTyr: 2.112 ± 0.346
0.0IleXaa: 0.0 ± 0.0
Lys
4.306LysAla: 4.306 ± 0.69
0.331LysCys: 0.331 ± 0.124
3.437LysAsp: 3.437 ± 0.445
2.774LysGlu: 2.774 ± 0.316
2.402LysPhe: 2.402 ± 0.338
3.313LysGly: 3.313 ± 0.462
0.994LysHis: 0.994 ± 0.183
3.313LysIle: 3.313 ± 0.397
3.934LysLys: 3.934 ± 0.505
4.141LysLeu: 4.141 ± 0.404
1.905LysMet: 1.905 ± 0.264
2.402LysAsn: 2.402 ± 0.309
2.609LysPro: 2.609 ± 0.367
2.029LysGln: 2.029 ± 0.287
3.064LysArg: 3.064 ± 0.329
3.023LysSer: 3.023 ± 0.312
2.857LysThr: 2.857 ± 0.349
3.354LysVal: 3.354 ± 0.319
0.911LysTrp: 0.911 ± 0.171
1.656LysTyr: 1.656 ± 0.287
0.0LysXaa: 0.0 ± 0.0
Leu
6.625LeuAla: 6.625 ± 0.664
0.663LeuCys: 0.663 ± 0.219
5.135LeuAsp: 5.135 ± 0.496
4.596LeuGlu: 4.596 ± 0.359
2.484LeuPhe: 2.484 ± 0.286
5.88LeuGly: 5.88 ± 0.434
1.118LeuHis: 1.118 ± 0.313
3.644LeuIle: 3.644 ± 0.43
3.934LeuLys: 3.934 ± 0.426
5.342LeuLeu: 5.342 ± 0.444
1.739LeuMet: 1.739 ± 0.312
3.81LeuAsn: 3.81 ± 0.459
3.727LeuPro: 3.727 ± 0.365
2.029LeuGln: 2.029 ± 0.256
3.23LeuArg: 3.23 ± 0.375
4.845LeuSer: 4.845 ± 0.512
5.217LeuThr: 5.217 ± 0.404
4.803LeuVal: 4.803 ± 0.479
0.704LeuTrp: 0.704 ± 0.15
2.029LeuTyr: 2.029 ± 0.291
0.0LeuXaa: 0.0 ± 0.0
Met
2.65MetAla: 2.65 ± 0.316
0.207MetCys: 0.207 ± 0.079
1.035MetAsp: 1.035 ± 0.288
1.491MetGlu: 1.491 ± 0.233
0.87MetPhe: 0.87 ± 0.178
1.781MetGly: 1.781 ± 0.242
0.373MetHis: 0.373 ± 0.147
1.573MetIle: 1.573 ± 0.278
1.408MetLys: 1.408 ± 0.218
1.698MetLeu: 1.698 ± 0.217
0.331MetMet: 0.331 ± 0.137
1.449MetAsn: 1.449 ± 0.256
1.242MetPro: 1.242 ± 0.228
0.994MetGln: 0.994 ± 0.261
1.698MetArg: 1.698 ± 0.237
2.484MetSer: 2.484 ± 0.259
2.112MetThr: 2.112 ± 0.328
1.615MetVal: 1.615 ± 0.252
0.248MetTrp: 0.248 ± 0.086
0.414MetTyr: 0.414 ± 0.121
0.0MetXaa: 0.0 ± 0.0
Asn
3.437AsnAla: 3.437 ± 0.491
0.166AsnCys: 0.166 ± 0.087
2.94AsnAsp: 2.94 ± 0.453
2.153AsnGlu: 2.153 ± 0.32
2.277AsnPhe: 2.277 ± 0.321
4.058AsnGly: 4.058 ± 0.492
0.952AsnHis: 0.952 ± 0.195
2.857AsnIle: 2.857 ± 0.417
2.236AsnLys: 2.236 ± 0.285
3.437AsnLeu: 3.437 ± 0.44
1.077AsnMet: 1.077 ± 0.202
2.277AsnAsn: 2.277 ± 0.349
2.816AsnPro: 2.816 ± 0.304
1.573AsnGln: 1.573 ± 0.267
2.774AsnArg: 2.774 ± 0.34
2.94AsnSer: 2.94 ± 0.439
3.064AsnThr: 3.064 ± 0.299
2.774AsnVal: 2.774 ± 0.375
0.704AsnTrp: 0.704 ± 0.187
1.201AsnTyr: 1.201 ± 0.159
0.0AsnXaa: 0.0 ± 0.0
Pro
6.211ProAla: 6.211 ± 0.814
0.331ProCys: 0.331 ± 0.126
3.23ProAsp: 3.23 ± 0.377
4.638ProGlu: 4.638 ± 0.582
1.698ProPhe: 1.698 ± 0.276
4.472ProGly: 4.472 ± 0.392
1.284ProHis: 1.284 ± 0.256
2.07ProIle: 2.07 ± 0.33
2.195ProLys: 2.195 ± 0.321
3.106ProLeu: 3.106 ± 0.411
1.201ProMet: 1.201 ± 0.222
2.236ProAsn: 2.236 ± 0.326
2.899ProPro: 2.899 ± 0.464
1.532ProGln: 1.532 ± 0.241
2.526ProArg: 2.526 ± 0.319
2.981ProSer: 2.981 ± 0.378
3.395ProThr: 3.395 ± 0.502
3.478ProVal: 3.478 ± 0.401
0.994ProTrp: 0.994 ± 0.202
1.284ProTyr: 1.284 ± 0.259
0.0ProXaa: 0.0 ± 0.0
Gln
3.768GlnAla: 3.768 ± 0.716
0.29GlnCys: 0.29 ± 0.139
2.277GlnAsp: 2.277 ± 0.281
2.36GlnGlu: 2.36 ± 0.306
1.449GlnPhe: 1.449 ± 0.237
2.609GlnGly: 2.609 ± 0.291
0.787GlnHis: 0.787 ± 0.185
2.195GlnIle: 2.195 ± 0.328
2.112GlnLys: 2.112 ± 0.386
2.484GlnLeu: 2.484 ± 0.263
1.035GlnMet: 1.035 ± 0.202
1.615GlnAsn: 1.615 ± 0.226
1.739GlnPro: 1.739 ± 0.299
1.739GlnGln: 1.739 ± 0.314
1.822GlnArg: 1.822 ± 0.263
2.07GlnSer: 2.07 ± 0.276
1.905GlnThr: 1.905 ± 0.323
2.112GlnVal: 2.112 ± 0.327
0.704GlnTrp: 0.704 ± 0.186
1.656GlnTyr: 1.656 ± 0.19
0.0GlnXaa: 0.0 ± 0.0
Arg
4.679ArgAla: 4.679 ± 0.465
0.663ArgCys: 0.663 ± 0.191
3.727ArgAsp: 3.727 ± 0.417
3.81ArgGlu: 3.81 ± 0.452
2.236ArgPhe: 2.236 ± 0.275
3.81ArgGly: 3.81 ± 0.402
1.035ArgHis: 1.035 ± 0.226
3.437ArgIle: 3.437 ± 0.342
3.106ArgLys: 3.106 ± 0.452
3.602ArgLeu: 3.602 ± 0.372
1.698ArgMet: 1.698 ± 0.292
3.023ArgAsn: 3.023 ± 0.342
1.863ArgPro: 1.863 ± 0.325
1.781ArgGln: 1.781 ± 0.198
3.354ArgArg: 3.354 ± 0.522
2.443ArgSer: 2.443 ± 0.399
2.981ArgThr: 2.981 ± 0.275
3.81ArgVal: 3.81 ± 0.519
1.242ArgTrp: 1.242 ± 0.236
1.615ArgTyr: 1.615 ± 0.302
0.0ArgXaa: 0.0 ± 0.0
Ser
5.839SerAla: 5.839 ± 0.497
0.745SerCys: 0.745 ± 0.188
3.81SerAsp: 3.81 ± 0.378
3.023SerGlu: 3.023 ± 0.462
2.816SerPhe: 2.816 ± 0.381
7.371SerGly: 7.371 ± 0.735
1.366SerHis: 1.366 ± 0.24
3.147SerIle: 3.147 ± 0.479
2.609SerLys: 2.609 ± 0.307
5.342SerLeu: 5.342 ± 0.446
1.615SerMet: 1.615 ± 0.253
2.402SerAsn: 2.402 ± 0.326
3.064SerPro: 3.064 ± 0.408
1.988SerGln: 1.988 ± 0.278
3.478SerArg: 3.478 ± 0.429
3.768SerSer: 3.768 ± 0.359
3.52SerThr: 3.52 ± 0.433
4.472SerVal: 4.472 ± 0.483
0.911SerTrp: 0.911 ± 0.199
1.491SerTyr: 1.491 ± 0.293
0.0SerXaa: 0.0 ± 0.0
Thr
6.791ThrAla: 6.791 ± 0.948
0.58ThrCys: 0.58 ± 0.189
4.017ThrAsp: 4.017 ± 0.513
3.685ThrGlu: 3.685 ± 0.413
1.781ThrPhe: 1.781 ± 0.239
6.915ThrGly: 6.915 ± 0.606
0.828ThrHis: 0.828 ± 0.17
2.733ThrIle: 2.733 ± 0.295
2.774ThrLys: 2.774 ± 0.295
4.017ThrLeu: 4.017 ± 0.421
1.449ThrMet: 1.449 ± 0.248
1.863ThrAsn: 1.863 ± 0.263
3.851ThrPro: 3.851 ± 0.5
2.65ThrGln: 2.65 ± 0.281
2.816ThrArg: 2.816 ± 0.331
3.064ThrSer: 3.064 ± 0.384
3.354ThrThr: 3.354 ± 0.407
4.679ThrVal: 4.679 ± 0.484
1.242ThrTrp: 1.242 ± 0.207
2.112ThrTyr: 2.112 ± 0.321
0.0ThrXaa: 0.0 ± 0.0
Val
5.424ValAla: 5.424 ± 0.534
0.663ValCys: 0.663 ± 0.169
4.348ValAsp: 4.348 ± 0.453
4.058ValGlu: 4.058 ± 0.436
2.36ValPhe: 2.36 ± 0.316
4.389ValGly: 4.389 ± 0.477
1.449ValHis: 1.449 ± 0.263
3.934ValIle: 3.934 ± 0.393
2.899ValLys: 2.899 ± 0.301
4.224ValLeu: 4.224 ± 0.457
1.408ValMet: 1.408 ± 0.229
2.774ValAsn: 2.774 ± 0.295
3.851ValPro: 3.851 ± 0.415
2.857ValGln: 2.857 ± 0.387
3.975ValArg: 3.975 ± 0.511
4.099ValSer: 4.099 ± 0.499
4.017ValThr: 4.017 ± 0.51
4.099ValVal: 4.099 ± 0.358
0.787ValTrp: 0.787 ± 0.197
2.402ValTyr: 2.402 ± 0.305
0.0ValXaa: 0.0 ± 0.0
Trp
1.159TrpAla: 1.159 ± 0.234
0.207TrpCys: 0.207 ± 0.085
1.449TrpAsp: 1.449 ± 0.216
1.035TrpGlu: 1.035 ± 0.238
0.58TrpPhe: 0.58 ± 0.134
1.118TrpGly: 1.118 ± 0.215
0.455TrpHis: 0.455 ± 0.158
0.911TrpIle: 0.911 ± 0.185
0.745TrpLys: 0.745 ± 0.152
1.284TrpLeu: 1.284 ± 0.214
0.331TrpMet: 0.331 ± 0.128
0.994TrpAsn: 0.994 ± 0.18
0.497TrpPro: 0.497 ± 0.14
0.621TrpGln: 0.621 ± 0.171
1.077TrpArg: 1.077 ± 0.199
1.366TrpSer: 1.366 ± 0.216
0.621TrpThr: 0.621 ± 0.189
0.828TrpVal: 0.828 ± 0.18
0.207TrpTrp: 0.207 ± 0.104
0.621TrpTyr: 0.621 ± 0.161
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.153TyrAla: 2.153 ± 0.329
0.29TyrCys: 0.29 ± 0.13
2.195TyrAsp: 2.195 ± 0.347
1.905TyrGlu: 1.905 ± 0.304
1.284TyrPhe: 1.284 ± 0.258
3.478TyrGly: 3.478 ± 0.292
0.414TyrHis: 0.414 ± 0.144
1.781TyrIle: 1.781 ± 0.274
1.822TyrLys: 1.822 ± 0.249
2.112TyrLeu: 2.112 ± 0.339
0.621TyrMet: 0.621 ± 0.193
0.87TyrAsn: 0.87 ± 0.219
1.159TyrPro: 1.159 ± 0.275
1.118TyrGln: 1.118 ± 0.197
2.277TyrArg: 2.277 ± 0.292
1.781TyrSer: 1.781 ± 0.299
2.029TyrThr: 2.029 ± 0.313
2.153TyrVal: 2.153 ± 0.336
0.538TyrTrp: 0.538 ± 0.119
1.284TyrTyr: 1.284 ± 0.264
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 102 proteins (24151 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski