Amino acid dipepetide frequency for Weissella phage PWc

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.978AlaAla: 2.978 ± 1.315
0.175AlaCys: 0.175 ± 0.14
4.379AlaAsp: 4.379 ± 0.599
4.204AlaGlu: 4.204 ± 0.597
3.504AlaPhe: 3.504 ± 0.575
5.343AlaGly: 5.343 ± 1.349
1.051AlaHis: 1.051 ± 0.323
4.993AlaIle: 4.993 ± 0.629
4.117AlaLys: 4.117 ± 0.621
6.482AlaLeu: 6.482 ± 1.107
2.628AlaMet: 2.628 ± 0.914
4.467AlaAsn: 4.467 ± 0.649
2.452AlaPro: 2.452 ± 0.392
4.117AlaGln: 4.117 ± 0.7
2.803AlaArg: 2.803 ± 0.556
4.117AlaSer: 4.117 ± 0.866
4.642AlaThr: 4.642 ± 0.616
3.854AlaVal: 3.854 ± 0.569
0.876AlaTrp: 0.876 ± 0.239
2.89AlaTyr: 2.89 ± 0.383
0.0AlaXaa: 0.0 ± 0.0
Cys
0.35CysAla: 0.35 ± 0.165
0.0CysCys: 0.0 ± 0.0
0.175CysAsp: 0.175 ± 0.129
0.438CysGlu: 0.438 ± 0.217
0.0CysPhe: 0.0 ± 0.0
0.0CysGly: 0.0 ± 0.0
0.088CysHis: 0.088 ± 0.084
0.175CysIle: 0.175 ± 0.124
0.175CysLys: 0.175 ± 0.147
0.088CysLeu: 0.088 ± 0.094
0.175CysMet: 0.175 ± 0.134
0.175CysAsn: 0.175 ± 0.119
0.0CysPro: 0.0 ± 0.0
0.175CysGln: 0.175 ± 0.126
0.175CysArg: 0.175 ± 0.136
0.175CysSer: 0.175 ± 0.118
0.175CysThr: 0.175 ± 0.117
0.263CysVal: 0.263 ± 0.197
0.088CysTrp: 0.088 ± 0.086
0.175CysTyr: 0.175 ± 0.123
0.0CysXaa: 0.0 ± 0.0
Asp
5.08AspAla: 5.08 ± 0.743
0.263AspCys: 0.263 ± 0.206
3.854AspAsp: 3.854 ± 0.795
5.168AspGlu: 5.168 ± 0.826
3.241AspPhe: 3.241 ± 0.574
4.379AspGly: 4.379 ± 0.722
0.35AspHis: 0.35 ± 0.175
5.693AspIle: 5.693 ± 0.7
5.781AspLys: 5.781 ± 0.757
5.606AspLeu: 5.606 ± 0.792
2.365AspMet: 2.365 ± 0.426
4.73AspAsn: 4.73 ± 0.647
1.664AspPro: 1.664 ± 0.513
1.577AspGln: 1.577 ± 0.302
1.839AspArg: 1.839 ± 0.439
2.452AspSer: 2.452 ± 0.626
3.941AspThr: 3.941 ± 0.602
4.117AspVal: 4.117 ± 0.643
0.438AspTrp: 0.438 ± 0.224
2.54AspTyr: 2.54 ± 0.521
0.0AspXaa: 0.0 ± 0.0
Glu
5.255GluAla: 5.255 ± 0.991
0.35GluCys: 0.35 ± 0.16
3.328GluAsp: 3.328 ± 0.669
5.255GluGlu: 5.255 ± 0.826
2.715GluPhe: 2.715 ± 0.47
3.766GluGly: 3.766 ± 0.548
1.314GluHis: 1.314 ± 0.299
5.168GluIle: 5.168 ± 0.846
5.693GluLys: 5.693 ± 0.935
7.708GluLeu: 7.708 ± 0.943
2.452GluMet: 2.452 ± 0.458
4.555GluAsn: 4.555 ± 0.668
1.489GluPro: 1.489 ± 0.379
3.241GluGln: 3.241 ± 0.506
2.803GluArg: 2.803 ± 0.512
2.54GluSer: 2.54 ± 0.459
4.379GluThr: 4.379 ± 0.62
5.08GluVal: 5.08 ± 0.659
1.752GluTrp: 1.752 ± 0.415
3.591GluTyr: 3.591 ± 0.71
0.0GluXaa: 0.0 ± 0.0
Phe
2.19PheAla: 2.19 ± 0.385
0.0PheCys: 0.0 ± 0.0
2.89PheAsp: 2.89 ± 0.409
3.153PheGlu: 3.153 ± 0.52
0.701PhePhe: 0.701 ± 0.262
3.504PheGly: 3.504 ± 0.553
0.526PheHis: 0.526 ± 0.183
2.365PheIle: 2.365 ± 0.314
2.803PheLys: 2.803 ± 0.554
2.452PheLeu: 2.452 ± 0.448
1.401PheMet: 1.401 ± 0.366
3.679PheAsn: 3.679 ± 0.455
1.226PhePro: 1.226 ± 0.359
0.526PheGln: 0.526 ± 0.209
0.963PheArg: 0.963 ± 0.243
2.19PheSer: 2.19 ± 0.342
3.328PheThr: 3.328 ± 0.547
2.452PheVal: 2.452 ± 0.388
0.263PheTrp: 0.263 ± 0.138
1.489PheTyr: 1.489 ± 0.371
0.0PheXaa: 0.0 ± 0.0
Gly
4.905GlyAla: 4.905 ± 1.279
0.088GlyCys: 0.088 ± 0.086
4.817GlyAsp: 4.817 ± 0.881
3.328GlyGlu: 3.328 ± 0.741
2.89GlyPhe: 2.89 ± 0.566
5.693GlyGly: 5.693 ± 0.88
0.963GlyHis: 0.963 ± 0.323
4.73GlyIle: 4.73 ± 1.057
6.482GlyLys: 6.482 ± 0.856
6.394GlyLeu: 6.394 ± 0.713
1.839GlyMet: 1.839 ± 0.357
4.73GlyAsn: 4.73 ± 0.599
0.175GlyPro: 0.175 ± 0.111
1.664GlyGln: 1.664 ± 0.422
2.277GlyArg: 2.277 ± 0.558
5.08GlySer: 5.08 ± 0.666
5.343GlyThr: 5.343 ± 0.817
6.044GlyVal: 6.044 ± 0.763
0.876GlyTrp: 0.876 ± 0.242
3.153GlyTyr: 3.153 ± 0.497
0.0GlyXaa: 0.0 ± 0.0
His
0.963HisAla: 0.963 ± 0.314
0.0HisCys: 0.0 ± 0.0
0.788HisAsp: 0.788 ± 0.298
0.963HisGlu: 0.963 ± 0.287
0.526HisPhe: 0.526 ± 0.251
1.401HisGly: 1.401 ± 0.282
0.175HisHis: 0.175 ± 0.119
0.963HisIle: 0.963 ± 0.25
1.314HisLys: 1.314 ± 0.278
1.314HisLeu: 1.314 ± 0.391
0.526HisMet: 0.526 ± 0.182
0.963HisAsn: 0.963 ± 0.286
0.438HisPro: 0.438 ± 0.223
0.175HisGln: 0.175 ± 0.126
0.35HisArg: 0.35 ± 0.167
0.526HisSer: 0.526 ± 0.226
1.226HisThr: 1.226 ± 0.333
0.963HisVal: 0.963 ± 0.244
0.175HisTrp: 0.175 ± 0.128
0.438HisTyr: 0.438 ± 0.178
0.0HisXaa: 0.0 ± 0.0
Ile
5.168IleAla: 5.168 ± 0.929
0.35IleCys: 0.35 ± 0.165
4.379IleAsp: 4.379 ± 0.476
5.868IleGlu: 5.868 ± 0.653
1.314IlePhe: 1.314 ± 0.366
5.956IleGly: 5.956 ± 1.232
0.788IleHis: 0.788 ± 0.257
4.029IleIle: 4.029 ± 0.609
5.08IleLys: 5.08 ± 0.708
4.117IleLeu: 4.117 ± 0.544
2.277IleMet: 2.277 ± 0.435
4.204IleAsn: 4.204 ± 0.811
1.927IlePro: 1.927 ± 0.444
1.839IleGln: 1.839 ± 0.38
1.489IleArg: 1.489 ± 0.321
5.255IleSer: 5.255 ± 0.771
4.467IleThr: 4.467 ± 0.407
5.255IleVal: 5.255 ± 0.705
0.526IleTrp: 0.526 ± 0.248
3.153IleTyr: 3.153 ± 0.643
0.0IleXaa: 0.0 ± 0.0
Lys
5.781LysAla: 5.781 ± 0.974
0.0LysCys: 0.0 ± 0.0
5.518LysAsp: 5.518 ± 0.726
5.255LysGlu: 5.255 ± 0.88
3.066LysPhe: 3.066 ± 0.584
4.905LysGly: 4.905 ± 0.732
0.876LysHis: 0.876 ± 0.325
4.204LysIle: 4.204 ± 0.492
5.343LysLys: 5.343 ± 0.705
5.693LysLeu: 5.693 ± 0.88
1.927LysMet: 1.927 ± 0.325
4.467LysAsn: 4.467 ± 0.724
2.452LysPro: 2.452 ± 0.445
2.803LysGln: 2.803 ± 0.51
2.715LysArg: 2.715 ± 0.507
4.73LysSer: 4.73 ± 0.7
4.204LysThr: 4.204 ± 0.493
5.168LysVal: 5.168 ± 0.618
1.226LysTrp: 1.226 ± 0.382
3.153LysTyr: 3.153 ± 0.426
0.0LysXaa: 0.0 ± 0.0
Leu
5.255LeuAla: 5.255 ± 0.781
0.175LeuCys: 0.175 ± 0.14
6.131LeuAsp: 6.131 ± 0.724
4.555LeuGlu: 4.555 ± 0.612
3.416LeuPhe: 3.416 ± 0.584
5.43LeuGly: 5.43 ± 0.96
1.051LeuHis: 1.051 ± 0.39
5.781LeuIle: 5.781 ± 0.774
5.43LeuLys: 5.43 ± 0.647
5.606LeuLeu: 5.606 ± 0.72
1.752LeuMet: 1.752 ± 0.517
4.993LeuAsn: 4.993 ± 0.681
3.328LeuPro: 3.328 ± 0.417
2.89LeuGln: 2.89 ± 0.439
3.241LeuArg: 3.241 ± 0.519
6.482LeuSer: 6.482 ± 0.934
4.642LeuThr: 4.642 ± 0.534
5.343LeuVal: 5.343 ± 0.576
1.051LeuTrp: 1.051 ± 0.327
3.679LeuTyr: 3.679 ± 0.474
0.0LeuXaa: 0.0 ± 0.0
Met
2.89MetAla: 2.89 ± 0.683
0.088MetCys: 0.088 ± 0.092
1.839MetAsp: 1.839 ± 0.403
1.752MetGlu: 1.752 ± 0.439
1.051MetPhe: 1.051 ± 0.314
1.664MetGly: 1.664 ± 0.396
0.175MetHis: 0.175 ± 0.163
1.489MetIle: 1.489 ± 0.35
2.628MetLys: 2.628 ± 0.542
2.452MetLeu: 2.452 ± 0.495
0.788MetMet: 0.788 ± 0.226
1.839MetAsn: 1.839 ± 0.364
0.788MetPro: 0.788 ± 0.285
1.401MetGln: 1.401 ± 0.507
1.139MetArg: 1.139 ± 0.25
1.577MetSer: 1.577 ± 0.368
2.277MetThr: 2.277 ± 0.391
2.365MetVal: 2.365 ± 0.502
0.263MetTrp: 0.263 ± 0.149
1.314MetTyr: 1.314 ± 0.394
0.0MetXaa: 0.0 ± 0.0
Asn
4.555AsnAla: 4.555 ± 0.677
0.438AsnCys: 0.438 ± 0.189
2.978AsnAsp: 2.978 ± 0.442
5.606AsnGlu: 5.606 ± 0.681
1.927AsnPhe: 1.927 ± 0.347
5.343AsnGly: 5.343 ± 0.719
1.139AsnHis: 1.139 ± 0.263
4.117AsnIle: 4.117 ± 0.885
4.029AsnLys: 4.029 ± 0.855
5.343AsnLeu: 5.343 ± 0.518
1.664AsnMet: 1.664 ± 0.399
5.43AsnAsn: 5.43 ± 0.822
3.241AsnPro: 3.241 ± 0.518
2.102AsnGln: 2.102 ± 0.488
2.54AsnArg: 2.54 ± 0.548
3.153AsnSer: 3.153 ± 0.497
4.467AsnThr: 4.467 ± 0.532
3.941AsnVal: 3.941 ± 0.546
0.788AsnTrp: 0.788 ± 0.283
2.978AsnTyr: 2.978 ± 0.422
0.0AsnXaa: 0.0 ± 0.0
Pro
1.664ProAla: 1.664 ± 0.462
0.088ProCys: 0.088 ± 0.086
2.277ProAsp: 2.277 ± 0.486
2.277ProGlu: 2.277 ± 0.404
1.664ProPhe: 1.664 ± 0.388
0.263ProGly: 0.263 ± 0.156
0.526ProHis: 0.526 ± 0.241
1.927ProIle: 1.927 ± 0.426
2.102ProLys: 2.102 ± 0.415
2.102ProLeu: 2.102 ± 0.407
0.788ProMet: 0.788 ± 0.292
2.803ProAsn: 2.803 ± 0.537
0.35ProPro: 0.35 ± 0.177
0.963ProGln: 0.963 ± 0.386
1.401ProArg: 1.401 ± 0.289
1.226ProSer: 1.226 ± 0.27
1.314ProThr: 1.314 ± 0.351
3.416ProVal: 3.416 ± 0.509
0.526ProTrp: 0.526 ± 0.249
0.788ProTyr: 0.788 ± 0.305
0.0ProXaa: 0.0 ± 0.0
Gln
3.766GlnAla: 3.766 ± 0.966
0.0GlnCys: 0.0 ± 0.0
1.839GlnAsp: 1.839 ± 0.423
2.015GlnGlu: 2.015 ± 0.556
0.876GlnPhe: 0.876 ± 0.263
3.328GlnGly: 3.328 ± 0.554
0.35GlnHis: 0.35 ± 0.178
2.19GlnIle: 2.19 ± 0.509
1.664GlnLys: 1.664 ± 0.381
2.54GlnLeu: 2.54 ± 0.421
1.226GlnMet: 1.226 ± 0.335
1.401GlnAsn: 1.401 ± 0.269
0.613GlnPro: 0.613 ± 0.232
1.401GlnGln: 1.401 ± 0.432
1.314GlnArg: 1.314 ± 0.35
1.577GlnSer: 1.577 ± 0.428
2.19GlnThr: 2.19 ± 0.408
2.54GlnVal: 2.54 ± 0.412
0.526GlnTrp: 0.526 ± 0.245
1.489GlnTyr: 1.489 ± 0.366
0.0GlnXaa: 0.0 ± 0.0
Arg
1.489ArgAla: 1.489 ± 0.42
0.175ArgCys: 0.175 ± 0.136
1.927ArgAsp: 1.927 ± 0.353
3.679ArgGlu: 3.679 ± 0.582
1.489ArgPhe: 1.489 ± 0.378
3.416ArgGly: 3.416 ± 0.565
0.35ArgHis: 0.35 ± 0.151
2.277ArgIle: 2.277 ± 0.35
2.365ArgLys: 2.365 ± 0.38
3.679ArgLeu: 3.679 ± 0.637
0.876ArgMet: 0.876 ± 0.26
2.978ArgAsn: 2.978 ± 0.572
0.788ArgPro: 0.788 ± 0.307
0.788ArgGln: 0.788 ± 0.311
1.664ArgArg: 1.664 ± 0.491
1.401ArgSer: 1.401 ± 0.269
1.927ArgThr: 1.927 ± 0.404
2.715ArgVal: 2.715 ± 0.494
0.088ArgTrp: 0.088 ± 0.086
2.19ArgTyr: 2.19 ± 0.62
0.0ArgXaa: 0.0 ± 0.0
Ser
4.029SerAla: 4.029 ± 0.723
0.088SerCys: 0.088 ± 0.094
4.467SerAsp: 4.467 ± 0.59
4.905SerGlu: 4.905 ± 0.779
1.927SerPhe: 1.927 ± 0.341
4.379SerGly: 4.379 ± 0.915
0.963SerHis: 0.963 ± 0.265
5.168SerIle: 5.168 ± 0.612
4.204SerLys: 4.204 ± 0.573
3.766SerLeu: 3.766 ± 0.561
1.839SerMet: 1.839 ± 0.56
2.978SerAsn: 2.978 ± 0.592
1.752SerPro: 1.752 ± 0.407
1.139SerGln: 1.139 ± 0.253
2.54SerArg: 2.54 ± 0.544
2.715SerSer: 2.715 ± 0.714
2.978SerThr: 2.978 ± 0.511
4.117SerVal: 4.117 ± 0.615
1.051SerTrp: 1.051 ± 0.329
1.664SerTyr: 1.664 ± 0.315
0.0SerXaa: 0.0 ± 0.0
Thr
5.956ThrAla: 5.956 ± 1.273
0.175ThrCys: 0.175 ± 0.135
4.555ThrAsp: 4.555 ± 0.749
3.504ThrGlu: 3.504 ± 0.608
2.452ThrPhe: 2.452 ± 0.412
4.905ThrGly: 4.905 ± 0.599
1.226ThrHis: 1.226 ± 0.393
3.679ThrIle: 3.679 ± 0.466
4.642ThrLys: 4.642 ± 0.449
6.131ThrLeu: 6.131 ± 0.952
1.489ThrMet: 1.489 ± 0.359
3.241ThrAsn: 3.241 ± 0.43
1.927ThrPro: 1.927 ± 0.305
2.015ThrGln: 2.015 ± 0.491
2.715ThrArg: 2.715 ± 0.448
3.153ThrSer: 3.153 ± 0.617
4.642ThrThr: 4.642 ± 0.835
4.905ThrVal: 4.905 ± 0.636
0.613ThrTrp: 0.613 ± 0.243
2.54ThrTyr: 2.54 ± 0.602
0.0ThrXaa: 0.0 ± 0.0
Val
4.555ValAla: 4.555 ± 0.612
0.088ValCys: 0.088 ± 0.093
4.555ValAsp: 4.555 ± 0.712
6.394ValGlu: 6.394 ± 0.662
3.153ValPhe: 3.153 ± 0.676
4.467ValGly: 4.467 ± 0.81
0.963ValHis: 0.963 ± 0.31
5.255ValIle: 5.255 ± 0.655
5.868ValLys: 5.868 ± 0.703
4.379ValLeu: 4.379 ± 0.588
2.19ValMet: 2.19 ± 0.397
4.905ValAsn: 4.905 ± 0.444
2.628ValPro: 2.628 ± 0.373
1.839ValGln: 1.839 ± 0.365
2.19ValArg: 2.19 ± 0.438
4.993ValSer: 4.993 ± 0.604
4.817ValThr: 4.817 ± 0.48
6.306ValVal: 6.306 ± 0.721
1.401ValTrp: 1.401 ± 0.32
3.066ValTyr: 3.066 ± 0.506
0.0ValXaa: 0.0 ± 0.0
Trp
0.701TrpAla: 0.701 ± 0.216
0.175TrpCys: 0.175 ± 0.136
0.876TrpAsp: 0.876 ± 0.278
0.876TrpGlu: 0.876 ± 0.278
0.701TrpPhe: 0.701 ± 0.255
0.963TrpGly: 0.963 ± 0.253
0.088TrpHis: 0.088 ± 0.084
0.438TrpIle: 0.438 ± 0.216
0.701TrpLys: 0.701 ± 0.193
1.577TrpLeu: 1.577 ± 0.369
0.35TrpMet: 0.35 ± 0.187
0.963TrpAsn: 0.963 ± 0.324
0.0TrpPro: 0.0 ± 0.0
0.526TrpGln: 0.526 ± 0.209
0.876TrpArg: 0.876 ± 0.255
0.701TrpSer: 0.701 ± 0.214
0.701TrpThr: 0.701 ± 0.248
0.963TrpVal: 0.963 ± 0.293
0.088TrpTrp: 0.088 ± 0.087
0.438TrpTyr: 0.438 ± 0.181
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.54TyrAla: 2.54 ± 0.462
0.35TyrCys: 0.35 ± 0.184
3.504TyrAsp: 3.504 ± 0.65
3.241TyrGlu: 3.241 ± 0.664
1.489TyrPhe: 1.489 ± 0.366
2.365TyrGly: 2.365 ± 0.558
1.226TyrHis: 1.226 ± 0.423
2.803TyrIle: 2.803 ± 0.599
3.066TyrLys: 3.066 ± 0.423
2.803TyrLeu: 2.803 ± 0.445
1.139TyrMet: 1.139 ± 0.326
2.365TyrAsn: 2.365 ± 0.432
1.226TyrPro: 1.226 ± 0.429
1.664TyrGln: 1.664 ± 0.374
1.226TyrArg: 1.226 ± 0.308
2.628TyrSer: 2.628 ± 0.461
2.803TyrThr: 2.803 ± 0.497
4.204TyrVal: 4.204 ± 0.697
0.088TyrTrp: 0.088 ± 0.092
2.715TyrTyr: 2.715 ± 0.603
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (11418 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski