Mycobacterium phage 40AC

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 90 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W8EHN8|W8EHN8_9CAUD Uncharacterized protein OS=Mycobacterium phage 40AC OX=1458717 GN=40AC_87 PE=4 SV=1
MM1 pKa = 7.46TKK3 pKa = 10.44FLLSLAAVGAIFGGMYY19 pKa = 10.12VFNDD23 pKa = 3.5AAEE26 pKa = 4.42AAPTVPFSQADD37 pKa = 3.96FPCQEE42 pKa = 4.63DD43 pKa = 4.07EE44 pKa = 4.53VLGYY48 pKa = 10.81APEE51 pKa = 4.63FGPDD55 pKa = 3.18SVGCIHH61 pKa = 7.76IDD63 pKa = 3.49LLKK66 pKa = 10.96GG67 pKa = 3.32

Molecular weight: 7.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W8EHG6|W8EHG6_9CAUD Terminase small subunit OS=Mycobacterium phage 40AC OX=1458717 GN=40AC_6 PE=4 SV=1
MM1 pKa = 7.18SRR3 pKa = 11.84HH4 pKa = 6.29RR5 pKa = 11.84IKK7 pKa = 9.72TQEE10 pKa = 3.82RR11 pKa = 11.84PNRR14 pKa = 11.84YY15 pKa = 6.82WWEE18 pKa = 4.21CPHH21 pKa = 6.54CGLRR25 pKa = 11.84MGHH28 pKa = 6.14PMKK31 pKa = 10.52ILARR35 pKa = 11.84VARR38 pKa = 11.84FNHH41 pKa = 5.77VGNCEE46 pKa = 3.82YY47 pKa = 10.64RR48 pKa = 11.84SYY50 pKa = 12.14NMM52 pKa = 4.53

Molecular weight: 6.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

90

0

90

16270

38

912

180.8

19.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.766 ± 0.324

0.848 ± 0.104

6.478 ± 0.164

6.921 ± 0.246

3.454 ± 0.187

8.5 ± 0.464

1.973 ± 0.14

4.917 ± 0.185

4.831 ± 0.245

8.168 ± 0.223

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.452 ± 0.144

3.282 ± 0.168

5.47 ± 0.237

3.608 ± 0.197

6.404 ± 0.334

5.353 ± 0.263

5.808 ± 0.246

7.019 ± 0.251

2.016 ± 0.135

2.729 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski