Amino acid dipepetide frequency for Mycobacterium phage 40AC

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.556AlaAla: 11.556 ± 0.859
0.615AlaCys: 0.615 ± 0.162
5.839AlaAsp: 5.839 ± 0.601
6.884AlaGlu: 6.884 ± 0.805
3.565AlaPhe: 3.565 ± 0.587
8.667AlaGly: 8.667 ± 0.787
1.721AlaHis: 1.721 ± 0.294
4.979AlaIle: 4.979 ± 0.64
5.225AlaLys: 5.225 ± 0.546
8.298AlaLeu: 8.298 ± 0.839
2.643AlaMet: 2.643 ± 0.394
3.012AlaAsn: 3.012 ± 0.418
4.18AlaPro: 4.18 ± 0.601
4.426AlaGln: 4.426 ± 0.631
5.962AlaArg: 5.962 ± 0.69
4.18AlaSer: 4.18 ± 0.507
4.241AlaThr: 4.241 ± 0.513
7.437AlaVal: 7.437 ± 0.656
1.66AlaTrp: 1.66 ± 0.367
2.582AlaTyr: 2.582 ± 0.39
0.0AlaXaa: 0.0 ± 0.0
Cys
0.676CysAla: 0.676 ± 0.17
0.123CysCys: 0.123 ± 0.076
0.553CysAsp: 0.553 ± 0.158
0.492CysGlu: 0.492 ± 0.155
0.369CysPhe: 0.369 ± 0.164
0.676CysGly: 0.676 ± 0.198
0.184CysHis: 0.184 ± 0.128
0.307CysIle: 0.307 ± 0.123
0.369CysLys: 0.369 ± 0.145
0.676CysLeu: 0.676 ± 0.189
0.184CysMet: 0.184 ± 0.105
0.369CysAsn: 0.369 ± 0.127
0.43CysPro: 0.43 ± 0.179
0.307CysGln: 0.307 ± 0.127
0.553CysArg: 0.553 ± 0.18
0.43CysSer: 0.43 ± 0.173
0.615CysThr: 0.615 ± 0.207
0.553CysVal: 0.553 ± 0.157
0.43CysTrp: 0.43 ± 0.178
0.184CysTyr: 0.184 ± 0.101
0.0CysXaa: 0.0 ± 0.0
Asp
7.007AspAla: 7.007 ± 0.687
0.369AspCys: 0.369 ± 0.165
3.627AspAsp: 3.627 ± 0.624
3.749AspGlu: 3.749 ± 0.556
2.889AspPhe: 2.889 ± 0.508
4.917AspGly: 4.917 ± 0.591
1.291AspHis: 1.291 ± 0.286
3.565AspIle: 3.565 ± 0.501
3.135AspLys: 3.135 ± 0.555
5.962AspLeu: 5.962 ± 0.735
1.475AspMet: 1.475 ± 0.284
1.106AspAsn: 1.106 ± 0.262
4.549AspPro: 4.549 ± 0.504
2.028AspGln: 2.028 ± 0.371
3.073AspArg: 3.073 ± 0.433
3.811AspSer: 3.811 ± 0.468
3.749AspThr: 3.749 ± 0.571
4.733AspVal: 4.733 ± 0.511
1.291AspTrp: 1.291 ± 0.25
2.459AspTyr: 2.459 ± 0.306
0.0AspXaa: 0.0 ± 0.0
Glu
6.515GluAla: 6.515 ± 0.678
0.307GluCys: 0.307 ± 0.144
4.18GluAsp: 4.18 ± 0.544
5.532GluGlu: 5.532 ± 0.817
2.95GluPhe: 2.95 ± 0.391
5.102GluGly: 5.102 ± 0.53
1.537GluHis: 1.537 ± 0.295
3.872GluIle: 3.872 ± 0.416
2.889GluLys: 2.889 ± 0.443
7.868GluLeu: 7.868 ± 0.725
1.721GluMet: 1.721 ± 0.297
1.967GluAsn: 1.967 ± 0.329
2.766GluPro: 2.766 ± 0.463
2.274GluGln: 2.274 ± 0.337
4.426GluArg: 4.426 ± 0.509
2.95GluSer: 2.95 ± 0.388
3.504GluThr: 3.504 ± 0.475
4.549GluVal: 4.549 ± 0.538
2.09GluTrp: 2.09 ± 0.347
2.213GluTyr: 2.213 ± 0.304
0.0GluXaa: 0.0 ± 0.0
Phe
2.889PheAla: 2.889 ± 0.406
0.246PheCys: 0.246 ± 0.134
2.95PheAsp: 2.95 ± 0.45
2.643PheGlu: 2.643 ± 0.357
0.492PhePhe: 0.492 ± 0.231
3.012PheGly: 3.012 ± 0.399
0.615PheHis: 0.615 ± 0.163
1.352PheIle: 1.352 ± 0.273
1.352PheLys: 1.352 ± 0.285
2.643PheLeu: 2.643 ± 0.523
0.615PheMet: 0.615 ± 0.221
2.09PheAsn: 2.09 ± 0.341
1.967PhePro: 1.967 ± 0.314
0.799PheGln: 0.799 ± 0.209
2.151PheArg: 2.151 ± 0.375
2.09PheSer: 2.09 ± 0.37
2.397PheThr: 2.397 ± 0.292
2.766PheVal: 2.766 ± 0.323
0.615PheTrp: 0.615 ± 0.178
0.799PheTyr: 0.799 ± 0.19
0.0PheXaa: 0.0 ± 0.0
Gly
6.147GlyAla: 6.147 ± 0.879
0.799GlyCys: 0.799 ± 0.186
6.577GlyAsp: 6.577 ± 1.05
4.979GlyGlu: 4.979 ± 0.5
3.381GlyPhe: 3.381 ± 0.532
7.806GlyGly: 7.806 ± 1.301
2.151GlyHis: 2.151 ± 0.401
4.487GlyIle: 4.487 ± 0.562
3.749GlyLys: 3.749 ± 0.444
7.13GlyLeu: 7.13 ± 0.909
2.459GlyMet: 2.459 ± 0.343
3.258GlyAsn: 3.258 ± 0.327
5.225GlyPro: 5.225 ± 1.371
3.196GlyGln: 3.196 ± 0.438
3.688GlyArg: 3.688 ± 0.462
3.995GlySer: 3.995 ± 0.506
5.348GlyThr: 5.348 ± 0.657
6.761GlyVal: 6.761 ± 0.563
1.352GlyTrp: 1.352 ± 0.308
2.52GlyTyr: 2.52 ± 0.35
0.0GlyXaa: 0.0 ± 0.0
His
1.598HisAla: 1.598 ± 0.289
0.369HisCys: 0.369 ± 0.14
1.229HisAsp: 1.229 ± 0.284
1.721HisGlu: 1.721 ± 0.341
0.676HisPhe: 0.676 ± 0.233
1.721HisGly: 1.721 ± 0.456
0.43HisHis: 0.43 ± 0.164
0.799HisIle: 0.799 ± 0.219
1.045HisLys: 1.045 ± 0.238
1.721HisLeu: 1.721 ± 0.336
0.184HisMet: 0.184 ± 0.113
0.553HisAsn: 0.553 ± 0.179
1.291HisPro: 1.291 ± 0.273
0.861HisGln: 0.861 ± 0.225
1.598HisArg: 1.598 ± 0.315
0.615HisSer: 0.615 ± 0.203
1.106HisThr: 1.106 ± 0.226
1.352HisVal: 1.352 ± 0.319
0.369HisTrp: 0.369 ± 0.167
0.492HisTyr: 0.492 ± 0.214
0.0HisXaa: 0.0 ± 0.0
Ile
4.549IleAla: 4.549 ± 0.457
0.246IleCys: 0.246 ± 0.105
3.688IleAsp: 3.688 ± 0.39
4.794IleGlu: 4.794 ± 0.536
1.537IlePhe: 1.537 ± 0.318
4.61IleGly: 4.61 ± 0.739
0.922IleHis: 0.922 ± 0.208
1.537IleIle: 1.537 ± 0.372
3.073IleLys: 3.073 ± 0.45
3.565IleLeu: 3.565 ± 0.428
0.615IleMet: 0.615 ± 0.211
2.397IleAsn: 2.397 ± 0.344
3.504IlePro: 3.504 ± 0.444
1.106IleGln: 1.106 ± 0.252
4.057IleArg: 4.057 ± 0.496
2.459IleSer: 2.459 ± 0.31
2.643IleThr: 2.643 ± 0.356
2.766IleVal: 2.766 ± 0.372
0.43IleTrp: 0.43 ± 0.163
0.676IleTyr: 0.676 ± 0.175
0.0IleXaa: 0.0 ± 0.0
Lys
5.04LysAla: 5.04 ± 0.477
0.184LysCys: 0.184 ± 0.114
1.967LysAsp: 1.967 ± 0.306
2.827LysGlu: 2.827 ± 0.421
1.291LysPhe: 1.291 ± 0.276
5.225LysGly: 5.225 ± 1.012
0.676LysHis: 0.676 ± 0.185
2.459LysIle: 2.459 ± 0.456
2.52LysLys: 2.52 ± 0.492
3.811LysLeu: 3.811 ± 0.604
1.045LysMet: 1.045 ± 0.227
1.66LysAsn: 1.66 ± 0.371
3.319LysPro: 3.319 ± 0.662
1.905LysGln: 1.905 ± 0.358
3.627LysArg: 3.627 ± 0.464
2.643LysSer: 2.643 ± 0.432
2.52LysThr: 2.52 ± 0.361
3.749LysVal: 3.749 ± 0.508
0.861LysTrp: 0.861 ± 0.211
0.983LysTyr: 0.983 ± 0.276
0.0LysXaa: 0.0 ± 0.0
Leu
8.359LeuAla: 8.359 ± 0.647
0.615LeuCys: 0.615 ± 0.195
5.409LeuAsp: 5.409 ± 0.562
5.04LeuGlu: 5.04 ± 0.596
2.827LeuPhe: 2.827 ± 0.328
6.024LeuGly: 6.024 ± 0.632
1.475LeuHis: 1.475 ± 0.323
4.549LeuIle: 4.549 ± 0.516
3.811LeuLys: 3.811 ± 0.607
5.348LeuLeu: 5.348 ± 0.609
2.213LeuMet: 2.213 ± 0.338
2.827LeuAsn: 2.827 ± 0.47
4.794LeuPro: 4.794 ± 0.521
2.643LeuGln: 2.643 ± 0.493
6.147LeuArg: 6.147 ± 0.796
6.27LeuSer: 6.27 ± 0.522
5.409LeuThr: 5.409 ± 0.632
4.487LeuVal: 4.487 ± 0.577
1.721LeuTrp: 1.721 ± 0.34
1.721LeuTyr: 1.721 ± 0.35
0.0LeuXaa: 0.0 ± 0.0
Met
3.258MetAla: 3.258 ± 0.418
0.123MetCys: 0.123 ± 0.083
0.861MetAsp: 0.861 ± 0.201
1.168MetGlu: 1.168 ± 0.229
0.553MetPhe: 0.553 ± 0.185
1.783MetGly: 1.783 ± 0.322
0.553MetHis: 0.553 ± 0.188
1.414MetIle: 1.414 ± 0.261
1.598MetLys: 1.598 ± 0.283
1.783MetLeu: 1.783 ± 0.304
0.738MetMet: 0.738 ± 0.268
0.676MetAsn: 0.676 ± 0.195
1.229MetPro: 1.229 ± 0.319
0.861MetGln: 0.861 ± 0.313
1.414MetArg: 1.414 ± 0.249
2.028MetSer: 2.028 ± 0.375
2.336MetThr: 2.336 ± 0.326
1.168MetVal: 1.168 ± 0.268
0.307MetTrp: 0.307 ± 0.119
0.676MetTyr: 0.676 ± 0.16
0.0MetXaa: 0.0 ± 0.0
Asn
2.705AsnAla: 2.705 ± 0.39
0.553AsnCys: 0.553 ± 0.182
2.213AsnAsp: 2.213 ± 0.355
2.459AsnGlu: 2.459 ± 0.386
0.738AsnPhe: 0.738 ± 0.261
3.135AsnGly: 3.135 ± 0.508
0.983AsnHis: 0.983 ± 0.219
1.537AsnIle: 1.537 ± 0.28
1.414AsnLys: 1.414 ± 0.352
3.012AsnLeu: 3.012 ± 0.402
0.615AsnMet: 0.615 ± 0.2
0.615AsnAsn: 0.615 ± 0.206
2.582AsnPro: 2.582 ± 0.408
0.922AsnGln: 0.922 ± 0.259
2.151AsnArg: 2.151 ± 0.364
1.783AsnSer: 1.783 ± 0.319
1.352AsnThr: 1.352 ± 0.259
2.213AsnVal: 2.213 ± 0.332
0.799AsnTrp: 0.799 ± 0.254
1.045AsnTyr: 1.045 ± 0.211
0.0AsnXaa: 0.0 ± 0.0
Pro
5.163ProAla: 5.163 ± 0.59
0.492ProCys: 0.492 ± 0.19
4.118ProAsp: 4.118 ± 0.441
5.04ProGlu: 5.04 ± 0.583
1.66ProPhe: 1.66 ± 0.341
4.733ProGly: 4.733 ± 0.556
1.106ProHis: 1.106 ± 0.314
2.274ProIle: 2.274 ± 0.344
3.012ProLys: 3.012 ± 0.716
3.319ProLeu: 3.319 ± 0.397
1.168ProMet: 1.168 ± 0.294
2.643ProAsn: 2.643 ± 0.43
2.274ProPro: 2.274 ± 0.423
2.459ProGln: 2.459 ± 0.395
3.442ProArg: 3.442 ± 0.581
2.52ProSer: 2.52 ± 0.371
3.135ProThr: 3.135 ± 0.436
3.688ProVal: 3.688 ± 0.496
1.106ProTrp: 1.106 ± 0.343
1.352ProTyr: 1.352 ± 0.303
0.0ProXaa: 0.0 ± 0.0
Gln
4.61GlnAla: 4.61 ± 0.627
0.123GlnCys: 0.123 ± 0.086
1.721GlnAsp: 1.721 ± 0.271
2.028GlnGlu: 2.028 ± 0.401
1.229GlnPhe: 1.229 ± 0.242
4.303GlnGly: 4.303 ± 0.976
0.861GlnHis: 0.861 ± 0.189
2.151GlnIle: 2.151 ± 0.324
1.721GlnLys: 1.721 ± 0.378
2.705GlnLeu: 2.705 ± 0.402
0.983GlnMet: 0.983 ± 0.295
0.738GlnAsn: 0.738 ± 0.199
1.414GlnPro: 1.414 ± 0.298
1.967GlnGln: 1.967 ± 0.331
2.52GlnArg: 2.52 ± 0.387
1.598GlnSer: 1.598 ± 0.43
1.844GlnThr: 1.844 ± 0.254
2.397GlnVal: 2.397 ± 0.274
0.492GlnTrp: 0.492 ± 0.168
0.676GlnTyr: 0.676 ± 0.176
0.0GlnXaa: 0.0 ± 0.0
Arg
5.163ArgAla: 5.163 ± 0.518
0.922ArgCys: 0.922 ± 0.272
3.627ArgAsp: 3.627 ± 0.459
4.733ArgGlu: 4.733 ± 0.556
2.705ArgPhe: 2.705 ± 0.468
4.549ArgGly: 4.549 ± 0.715
1.291ArgHis: 1.291 ± 0.27
3.811ArgIle: 3.811 ± 0.455
2.95ArgLys: 2.95 ± 0.481
5.655ArgLeu: 5.655 ± 0.444
1.905ArgMet: 1.905 ± 0.374
1.905ArgAsn: 1.905 ± 0.409
2.766ArgPro: 2.766 ± 0.364
2.028ArgGln: 2.028 ± 0.384
5.409ArgArg: 5.409 ± 0.674
3.258ArgSer: 3.258 ± 0.45
2.889ArgThr: 2.889 ± 0.417
4.487ArgVal: 4.487 ± 0.512
1.475ArgTrp: 1.475 ± 0.285
2.52ArgTyr: 2.52 ± 0.387
0.0ArgXaa: 0.0 ± 0.0
Ser
5.593SerAla: 5.593 ± 0.708
0.246SerCys: 0.246 ± 0.118
3.258SerAsp: 3.258 ± 0.378
3.565SerGlu: 3.565 ± 0.413
2.028SerPhe: 2.028 ± 0.336
4.241SerGly: 4.241 ± 0.58
0.738SerHis: 0.738 ± 0.202
2.336SerIle: 2.336 ± 0.279
2.582SerLys: 2.582 ± 0.398
3.872SerLeu: 3.872 ± 0.604
1.598SerMet: 1.598 ± 0.28
1.598SerAsn: 1.598 ± 0.278
2.827SerPro: 2.827 ± 0.424
2.151SerGln: 2.151 ± 0.331
3.811SerArg: 3.811 ± 0.542
2.766SerSer: 2.766 ± 0.513
3.872SerThr: 3.872 ± 0.452
3.811SerVal: 3.811 ± 0.472
1.229SerTrp: 1.229 ± 0.265
1.414SerTyr: 1.414 ± 0.312
0.0SerXaa: 0.0 ± 0.0
Thr
5.901ThrAla: 5.901 ± 0.468
0.369ThrCys: 0.369 ± 0.131
3.872ThrAsp: 3.872 ± 0.515
4.364ThrGlu: 4.364 ± 0.549
1.905ThrPhe: 1.905 ± 0.411
5.839ThrGly: 5.839 ± 0.886
0.799ThrHis: 0.799 ± 0.207
1.844ThrIle: 1.844 ± 0.357
2.459ThrLys: 2.459 ± 0.388
4.487ThrLeu: 4.487 ± 0.417
1.598ThrMet: 1.598 ± 0.322
1.66ThrAsn: 1.66 ± 0.261
3.995ThrPro: 3.995 ± 0.46
1.967ThrGln: 1.967 ± 0.328
2.827ThrArg: 2.827 ± 0.401
3.135ThrSer: 3.135 ± 0.465
3.565ThrThr: 3.565 ± 0.589
4.671ThrVal: 4.671 ± 0.515
1.229ThrTrp: 1.229 ± 0.26
1.598ThrTyr: 1.598 ± 0.285
0.0ThrXaa: 0.0 ± 0.0
Val
5.962ValAla: 5.962 ± 0.706
1.106ValCys: 1.106 ± 0.252
5.655ValAsp: 5.655 ± 0.612
4.241ValGlu: 4.241 ± 0.4
2.459ValPhe: 2.459 ± 0.465
5.655ValGly: 5.655 ± 0.521
1.229ValHis: 1.229 ± 0.3
3.995ValIle: 3.995 ± 0.514
3.688ValLys: 3.688 ± 0.471
5.655ValLeu: 5.655 ± 0.568
1.783ValMet: 1.783 ± 0.325
2.643ValAsn: 2.643 ± 0.43
2.766ValPro: 2.766 ± 0.523
2.274ValGln: 2.274 ± 0.378
3.995ValArg: 3.995 ± 0.481
3.872ValSer: 3.872 ± 0.495
4.487ValThr: 4.487 ± 0.651
5.225ValVal: 5.225 ± 0.517
1.598ValTrp: 1.598 ± 0.336
1.905ValTyr: 1.905 ± 0.305
0.0ValXaa: 0.0 ± 0.0
Trp
1.905TrpAla: 1.905 ± 0.332
0.43TrpCys: 0.43 ± 0.194
1.66TrpAsp: 1.66 ± 0.314
1.291TrpGlu: 1.291 ± 0.282
0.492TrpPhe: 0.492 ± 0.157
0.983TrpGly: 0.983 ± 0.268
0.738TrpHis: 0.738 ± 0.215
0.922TrpIle: 0.922 ± 0.26
0.861TrpLys: 0.861 ± 0.24
1.475TrpLeu: 1.475 ± 0.262
0.43TrpMet: 0.43 ± 0.169
0.553TrpAsn: 0.553 ± 0.198
1.229TrpPro: 1.229 ± 0.304
0.922TrpGln: 0.922 ± 0.248
1.168TrpArg: 1.168 ± 0.258
1.291TrpSer: 1.291 ± 0.317
1.106TrpThr: 1.106 ± 0.273
1.475TrpVal: 1.475 ± 0.329
0.553TrpTrp: 0.553 ± 0.18
0.676TrpTyr: 0.676 ± 0.203
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.073TyrAla: 3.073 ± 0.452
0.246TyrCys: 0.246 ± 0.118
1.783TyrAsp: 1.783 ± 0.263
1.66TyrGlu: 1.66 ± 0.29
0.799TyrPhe: 0.799 ± 0.207
2.028TyrGly: 2.028 ± 0.35
0.43TyrHis: 0.43 ± 0.174
0.983TyrIle: 0.983 ± 0.235
0.861TyrLys: 0.861 ± 0.263
2.705TyrLeu: 2.705 ± 0.448
0.492TyrMet: 0.492 ± 0.213
0.553TyrAsn: 0.553 ± 0.176
1.598TyrPro: 1.598 ± 0.31
0.983TyrGln: 0.983 ± 0.275
2.028TyrArg: 2.028 ± 0.412
1.844TyrSer: 1.844 ± 0.294
1.967TyrThr: 1.967 ± 0.378
1.905TyrVal: 1.905 ± 0.341
0.553TyrTrp: 0.553 ± 0.215
0.799TyrTyr: 0.799 ± 0.203
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 90 proteins (16270 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski