Hubei diptera virus 13

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEL1|A0A1L3KEL1_9VIRU RNA-directed RNA polymerase OS=Hubei diptera virus 13 OX=1922874 PE=4 SV=1
MM1 pKa = 7.45SVEE4 pKa = 4.21DD5 pKa = 4.08LVDD8 pKa = 4.34FVGEE12 pKa = 4.06TARR15 pKa = 11.84LEE17 pKa = 4.31VEE19 pKa = 4.39SQVGDD24 pKa = 3.56TLFSRR29 pKa = 11.84NGLKK33 pKa = 10.12EE34 pKa = 3.8RR35 pKa = 11.84AKK37 pKa = 10.62KK38 pKa = 9.82IALSATLLCARR49 pKa = 11.84ALLGTVLVLCCGAWATAAVMALVSVAKK76 pKa = 10.15HH77 pKa = 5.29AMWYY81 pKa = 10.21QYY83 pKa = 11.59DD84 pKa = 3.86SLYY87 pKa = 11.18KK88 pKa = 10.65LIVQSFVPEE97 pKa = 4.39PEE99 pKa = 4.28PEE101 pKa = 3.88PDD103 pKa = 3.61YY104 pKa = 11.17FSGLISDD111 pKa = 3.84VTALVKK117 pKa = 10.56RR118 pKa = 11.84VDD120 pKa = 3.73LTLLSLLLVVLMILASIWISMGYY143 pKa = 9.65VYY145 pKa = 11.22YY146 pKa = 11.01SMRR149 pKa = 11.84RR150 pKa = 11.84VSYY153 pKa = 9.82KK154 pKa = 10.19VRR156 pKa = 11.84GIQYY160 pKa = 9.59EE161 pKa = 3.97AMQAGSEE168 pKa = 4.11FRR170 pKa = 11.84EE171 pKa = 4.49GEE173 pKa = 4.08VPKK176 pKa = 10.65FQVRR180 pKa = 11.84VLLPGVFVNSHH191 pKa = 4.53QGYY194 pKa = 9.5GIRR197 pKa = 11.84VEE199 pKa = 3.97NWLVLPTHH207 pKa = 6.38VLTGSEE213 pKa = 3.84ILLEE217 pKa = 4.28GSRR220 pKa = 11.84GRR222 pKa = 11.84VVIPTDD228 pKa = 3.52HH229 pKa = 6.92EE230 pKa = 4.27PSKK233 pKa = 10.43LHH235 pKa = 7.04PDD237 pKa = 3.25VAYY240 pKa = 10.76VWLEE244 pKa = 3.67DD245 pKa = 4.13SQWTTLGTRR254 pKa = 11.84SAKK257 pKa = 9.62TIPITSCHH265 pKa = 5.28VRR267 pKa = 11.84CTGQKK272 pKa = 8.49GYY274 pKa = 11.99SMGTVNKK281 pKa = 9.18TNIIGVLRR289 pKa = 11.84YY290 pKa = 9.93SGSTVPGMSGAAYY303 pKa = 8.9VAQDD307 pKa = 3.55RR308 pKa = 11.84VVGMHH313 pKa = 6.78TGASMEE319 pKa = 4.28KK320 pKa = 9.88NLGVAAALFVNEE332 pKa = 4.62LKK334 pKa = 10.9KK335 pKa = 10.69KK336 pKa = 9.83IKK338 pKa = 10.6NEE340 pKa = 4.28AIFLPSSNNSEE351 pKa = 3.93DD352 pKa = 3.51AAYY355 pKa = 10.69QMKK358 pKa = 10.44SFKK361 pKa = 8.53NTKK364 pKa = 8.7WDD366 pKa = 3.57AKK368 pKa = 10.57LLEE371 pKa = 4.55ALAEE375 pKa = 4.32NADD378 pKa = 4.18DD379 pKa = 4.28MDD381 pKa = 4.65LGSWGGNVDD390 pKa = 6.25FDD392 pKa = 5.12PDD394 pKa = 3.4WKK396 pKa = 11.08AEE398 pKa = 4.13SAEE401 pKa = 4.19EE402 pKa = 3.93PKK404 pKa = 10.51KK405 pKa = 10.76QNPIEE410 pKa = 4.14LATLIMKK417 pKa = 8.52QHH419 pKa = 6.05GPSGEE424 pKa = 4.02EE425 pKa = 3.7TGFSLLNTKK434 pKa = 9.01VLEE437 pKa = 4.94DD438 pKa = 3.39IKK440 pKa = 11.06DD441 pKa = 3.59LQEE444 pKa = 3.88RR445 pKa = 11.84LTIVEE450 pKa = 4.69EE451 pKa = 4.29YY452 pKa = 10.38IKK454 pKa = 10.21TKK456 pKa = 10.11ISKK459 pKa = 10.1KK460 pKa = 10.14KK461 pKa = 8.11EE462 pKa = 3.68PKK464 pKa = 10.18PIVTYY469 pKa = 10.57DD470 pKa = 3.63CEE472 pKa = 4.11EE473 pKa = 4.69CGAKK477 pKa = 7.53TTSEE481 pKa = 4.05SDD483 pKa = 3.51LTKK486 pKa = 10.67HH487 pKa = 4.98QAKK490 pKa = 9.66HH491 pKa = 5.52VKK493 pKa = 9.26HH494 pKa = 6.24KK495 pKa = 11.27CEE497 pKa = 3.96ICDD500 pKa = 3.45ITCQTALRR508 pKa = 11.84LANHH512 pKa = 6.29VANAHH517 pKa = 5.43RR518 pKa = 11.84TAKK521 pKa = 10.39PEE523 pKa = 3.76SAYY526 pKa = 10.17PGDD529 pKa = 3.52ARR531 pKa = 11.84VVVQTAPEE539 pKa = 4.12TQAFLDD545 pKa = 4.0PRR547 pKa = 11.84SNSPKK552 pKa = 9.9RR553 pKa = 11.84KK554 pKa = 8.52NRR556 pKa = 11.84SSKK559 pKa = 10.23RR560 pKa = 11.84SSSTSSKK567 pKa = 10.59KK568 pKa = 9.86QDD570 pKa = 3.33SLPWEE575 pKa = 4.07KK576 pKa = 10.72LLYY579 pKa = 10.78QMVQSQNRR587 pKa = 11.84LEE589 pKa = 4.15EE590 pKa = 4.07YY591 pKa = 10.42LRR593 pKa = 11.84SEE595 pKa = 4.53RR596 pKa = 11.84RR597 pKa = 11.84AMAGPASATQPKK609 pKa = 9.85

Molecular weight: 67.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEK5|A0A1L3KEK5_9VIRU C2H2-type domain-containing protein OS=Hubei diptera virus 13 OX=1922874 PE=4 SV=1
MM1 pKa = 7.33VKK3 pKa = 10.12GKK5 pKa = 10.55SVVCPQCGRR14 pKa = 11.84RR15 pKa = 11.84FASQKK20 pKa = 10.83ALAQHH25 pKa = 6.87RR26 pKa = 11.84LAAHH30 pKa = 6.65AQPAPALRR38 pKa = 11.84RR39 pKa = 11.84AAKK42 pKa = 9.69RR43 pKa = 11.84VVRR46 pKa = 11.84SKK48 pKa = 10.84RR49 pKa = 11.84AGSSLSVQTVNGNDD63 pKa = 4.04RR64 pKa = 11.84IGHH67 pKa = 4.85WEE69 pKa = 3.81IPNNAGSGSLFSVIPIAPYY88 pKa = 9.29MLNDD92 pKa = 3.47TRR94 pKa = 11.84LQAHH98 pKa = 5.9SLLWARR104 pKa = 11.84WRR106 pKa = 11.84PVALKK111 pKa = 10.73VRR113 pKa = 11.84VSLAGASTTFGSVCVGWSPEE133 pKa = 3.89PDD135 pKa = 3.28FSKK138 pKa = 11.01ASSNSLNAQRR148 pKa = 11.84VLSLKK153 pKa = 9.89PSKK156 pKa = 9.66EE157 pKa = 3.53VRR159 pKa = 11.84AWEE162 pKa = 4.1TVEE165 pKa = 4.01MVLPVATDD173 pKa = 3.61RR174 pKa = 11.84KK175 pKa = 8.38WYY177 pKa = 7.75HH178 pKa = 4.68TTGMHH183 pKa = 5.92QEE185 pKa = 4.05ASHH188 pKa = 6.38GCLVVTIDD196 pKa = 4.0SAFGGYY202 pKa = 7.53TGKK205 pKa = 10.25IGFTAHH211 pKa = 7.27LMWTVQFEE219 pKa = 4.4GVEE222 pKa = 4.16LDD224 pKa = 3.89SVAGPVSKK232 pKa = 9.95DD233 pKa = 3.24TIQADD238 pKa = 3.98SGWSHH243 pKa = 7.3FFTTSDD249 pKa = 3.7SGWDD253 pKa = 3.36ATRR256 pKa = 11.84LTFKK260 pKa = 10.26EE261 pKa = 4.16SSGGSMVPFSSAKK274 pKa = 9.99PNFIYY279 pKa = 10.75EE280 pKa = 4.42PDD282 pKa = 3.25SSTRR286 pKa = 11.84VHH288 pKa = 6.21YY289 pKa = 10.67VRR291 pKa = 11.84EE292 pKa = 4.26DD293 pKa = 3.32GKK295 pKa = 11.09EE296 pKa = 3.5ADD298 pKa = 2.89AKK300 pKa = 10.56YY301 pKa = 9.88FVRR304 pKa = 11.84IQDD307 pKa = 3.35YY308 pKa = 7.52STPGLALCASIADD321 pKa = 3.58AKK323 pKa = 11.08AYY325 pKa = 9.54IQTGDD330 pKa = 3.44TTKK333 pKa = 11.09LLPYY337 pKa = 9.62KK338 pKa = 10.34SASSWVTPDD347 pKa = 3.3DD348 pKa = 3.86PKK350 pKa = 11.21FVGKK354 pKa = 9.07PAGSYY359 pKa = 8.11TAVRR363 pKa = 11.84PEE365 pKa = 3.95EE366 pKa = 3.77QDD368 pKa = 3.34LQSEE372 pKa = 4.59IEE374 pKa = 4.14RR375 pKa = 11.84LRR377 pKa = 11.84QQLQLLEE384 pKa = 4.54AGNEE388 pKa = 3.7KK389 pKa = 10.27WEE391 pKa = 4.23VLGSPP396 pKa = 4.47

Molecular weight: 43.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1443

396

609

481.0

53.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.524 ± 0.623

1.455 ± 0.1

5.128 ± 0.512

6.306 ± 0.589

3.049 ± 0.485

6.514 ± 0.348

2.356 ± 0.116

4.019 ± 0.393

6.999 ± 0.52

8.801 ± 0.574

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.772 ± 0.582

2.772 ± 0.271

4.92 ± 0.278

3.534 ± 0.418

5.821 ± 0.651

8.108 ± 1.319

5.336 ± 0.666

8.039 ± 0.215

2.356 ± 0.376

3.188 ± 0.254
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski