Pseudomonas sp. ok602

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; unclassified Pseudomonas

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4728 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I4SRV4|A0A1I4SRV4_9PSED Dihydroorotase OS=Pseudomonas sp. ok602 OX=1761898 GN=SAMN04487858_10580 PE=4 SV=1
TT1 pKa = 7.12GPITAGSFTGTYY13 pKa = 7.5GTLVLNANGTYY24 pKa = 9.92TYY26 pKa = 10.14TLHH29 pKa = 6.76PNEE32 pKa = 5.26AGFKK36 pKa = 9.95SLPGNGNGIEE46 pKa = 4.29TFTYY50 pKa = 10.35TLTDD54 pKa = 3.32ADD56 pKa = 4.37GDD58 pKa = 3.79QSIANLVLNVHH69 pKa = 6.31NNDD72 pKa = 4.25DD73 pKa = 4.14PVTLGGIKK81 pKa = 10.55LEE83 pKa = 4.5GSDD86 pKa = 3.22LTVYY90 pKa = 10.06EE91 pKa = 4.81KK92 pKa = 10.74NLPMGSDD99 pKa = 3.85PNNGALTQSGTFAVSAPDD117 pKa = 3.5GLKK120 pKa = 9.31TLTVQGITVVDD131 pKa = 3.94GGVTQTIPQSITTALGNKK149 pKa = 8.12LTITGYY155 pKa = 10.29DD156 pKa = 3.03ASTGVVSYY164 pKa = 10.86SYY166 pKa = 9.73TLTGTEE172 pKa = 3.89VHH174 pKa = 6.21PTGLGPNSIVEE185 pKa = 4.46SFTVIASDD193 pKa = 3.85GDD195 pKa = 3.85SGATNTATGTLEE207 pKa = 3.89VNIIDD212 pKa = 5.12DD213 pKa = 4.71LPTAKK218 pKa = 9.84PDD220 pKa = 3.2TGSAIEE226 pKa = 4.24GATLNLSVLGNDD238 pKa = 3.48IKK240 pKa = 11.27GSDD243 pKa = 3.67GAVVIGVRR251 pKa = 11.84GGTNTGTTASGGLNSNISGAYY272 pKa = 10.03GYY274 pKa = 8.01LTLDD278 pKa = 3.27AAGNAVYY285 pKa = 10.18HH286 pKa = 6.15SNPNSVTTGTVTDD299 pKa = 3.6TFVYY303 pKa = 10.25SIRR306 pKa = 11.84DD307 pKa = 3.19GDD309 pKa = 4.14GDD311 pKa = 3.85VSTTTITITINDD323 pKa = 3.79SGLNAVIDD331 pKa = 3.86NDD333 pKa = 3.54VTVYY337 pKa = 10.72EE338 pKa = 4.5KK339 pKa = 11.12ALDD342 pKa = 3.93LKK344 pKa = 11.4ADD346 pKa = 4.11GLDD349 pKa = 3.64LAPGTVIGSDD359 pKa = 3.42PSNTGEE365 pKa = 4.24TAGGTLVGSVTGGSGPLTYY384 pKa = 10.95SLVGSNAGLFGTIQVNADD402 pKa = 2.97GTYY405 pKa = 10.03TYY407 pKa = 10.02TLTKK411 pKa = 10.27AASTSPNANDD421 pKa = 5.0GVTTLNNADD430 pKa = 3.54SFIYY434 pKa = 10.32KK435 pKa = 9.01ATDD438 pKa = 3.07AQGNFVTGTILINIVDD454 pKa = 4.73DD455 pKa = 4.36VPSATASTRR464 pKa = 11.84SVTTVPISTNLMIVLDD480 pKa = 3.95VSGSMKK486 pKa = 10.16EE487 pKa = 3.92LSGVMINGVNQTRR500 pKa = 11.84LALAKK505 pKa = 10.02QAISALLDD513 pKa = 3.55KK514 pKa = 11.55YY515 pKa = 10.89EE516 pKa = 4.16GLGDD520 pKa = 3.8VMVQLVTFSSTAKK533 pKa = 10.49DD534 pKa = 3.54VTTTWVSAAVAKK546 pKa = 10.47AALTALIADD555 pKa = 4.55GGTNYY560 pKa = 10.46DD561 pKa = 3.28AAVAMMQTAFGQTGKK576 pKa = 10.53LAGAQNVGYY585 pKa = 9.35FFSDD589 pKa = 4.12GEE591 pKa = 4.17PSFGQDD597 pKa = 2.61IAPADD602 pKa = 3.76EE603 pKa = 4.61AALKK607 pKa = 9.94TFLTTNAINNYY618 pKa = 10.1AIGLGGGISGGSLDD632 pKa = 3.59PLAYY636 pKa = 10.58NGATGTNTNAVLVTDD651 pKa = 4.77LSQLNSVLSSSVVGVPVTGSLLEE674 pKa = 4.55GGSFGADD681 pKa = 2.53GGFIKK686 pKa = 10.71SIVVDD691 pKa = 4.09GTTYY695 pKa = 10.3TYY697 pKa = 11.1NPKK700 pKa = 9.1LTGSAALTFAGINHH714 pKa = 7.17GLYY717 pKa = 10.47NATDD721 pKa = 3.41HH722 pKa = 6.74SLTITTNNGGTLVIKK737 pKa = 10.42LDD739 pKa = 3.81SGDD742 pKa = 3.57YY743 pKa = 10.77SYY745 pKa = 11.38ISQKK749 pKa = 9.37YY750 pKa = 4.18TTTLITEE757 pKa = 4.19NFGFTLSDD765 pKa = 3.36NDD767 pKa = 3.62GDD769 pKa = 4.4LASSTLTVKK778 pKa = 10.17VLPNTAPVAVDD789 pKa = 2.89DD790 pKa = 4.69HH791 pKa = 7.15VITNTLFSNLTVPGEE806 pKa = 3.93LLLANDD812 pKa = 4.42SDD814 pKa = 4.69PNGDD818 pKa = 3.57RR819 pKa = 11.84LTSNPANFNTGSDD832 pKa = 3.7FRR834 pKa = 11.84GSGAITTFALPDD846 pKa = 3.48NLAANQNLADD856 pKa = 3.76VRR858 pKa = 11.84NAFAANVATMTAVLVISGYY877 pKa = 10.39LGEE880 pKa = 4.33VSGANANDD888 pKa = 3.49EE889 pKa = 4.6DD890 pKa = 4.77FISVKK895 pKa = 10.51LKK897 pKa = 10.83AGEE900 pKa = 4.23TLTLDD905 pKa = 3.29HH906 pKa = 6.72NLAGGHH912 pKa = 5.37VSMEE916 pKa = 3.93YY917 pKa = 10.6SLNGAGFLPISDD929 pKa = 4.05GGMITALTDD938 pKa = 2.95GTYY941 pKa = 10.15QIHH944 pKa = 5.44VTNITNTNGNNKK956 pKa = 8.87GAAEE960 pKa = 4.32SYY962 pKa = 9.88QLTMTVDD969 pKa = 3.38YY970 pKa = 10.57GAVHH974 pKa = 6.61GTTSDD979 pKa = 3.32YY980 pKa = 11.19HH981 pKa = 5.18GTYY984 pKa = 8.61TASDD988 pKa = 3.38NHH990 pKa = 6.58GGTDD994 pKa = 3.72GANVTLTYY1002 pKa = 10.4QAGHH1006 pKa = 6.42TLTGTSGDD1014 pKa = 4.55DD1015 pKa = 3.12ILVASGDD1022 pKa = 3.82NNLLNGGDD1030 pKa = 4.0GNDD1033 pKa = 3.23VLTVSGINNEE1043 pKa = 4.1LHH1045 pKa = 6.65GGAGNDD1051 pKa = 4.09LLFSGPGADD1060 pKa = 4.88LLDD1063 pKa = 4.41GGTGIDD1069 pKa = 3.47TASYY1073 pKa = 9.24AHH1075 pKa = 6.58ATAGVTVNLGTLIAQNTIGAGTDD1098 pKa = 3.13TLTSIEE1104 pKa = 4.21NLVGSNYY1111 pKa = 10.14NDD1113 pKa = 3.6TLTGDD1118 pKa = 3.39NGNNVINGGLGNDD1131 pKa = 3.86TLNGGGGDD1139 pKa = 4.55DD1140 pKa = 3.95ILIGGLGNNTLSGGAGADD1158 pKa = 3.36TFLWQQGNSGHH1169 pKa = 7.16DD1170 pKa = 3.38VVTDD1174 pKa = 3.95FTLGTDD1180 pKa = 4.27KK1181 pKa = 11.37LDD1183 pKa = 4.71LSQLLQGEE1191 pKa = 4.5NATTASLDD1199 pKa = 3.81DD1200 pKa = 3.89YY1201 pKa = 11.27LHH1203 pKa = 6.67FSVTVVGGAVVSSITVSSIAGAAANQTIDD1232 pKa = 3.48LAGVNLADD1240 pKa = 4.46HH1241 pKa = 6.53YY1242 pKa = 11.4GVTPGAGGAIASGHH1256 pKa = 5.79DD1257 pKa = 3.17TATIITGMLNDD1268 pKa = 4.5HH1269 pKa = 6.1SLKK1272 pKa = 10.51VDD1274 pKa = 3.57TVV1276 pKa = 3.29

Molecular weight: 128.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I4WF25|A0A1I4WF25_9PSED Branched-chain alpha-keto acid dehydrogenase E1 component OS=Pseudomonas sp. ok602 OX=1761898 GN=SAMN04487858_112149 PE=4 SV=1
MM1 pKa = 7.55LLASIFGVVMGVVLGLTGAGGGILAVPALVLGLGWNMTQAAPVALFAVGSAAAVGALDD59 pKa = 3.81GLRR62 pKa = 11.84HH63 pKa = 5.17GLVRR67 pKa = 11.84YY68 pKa = 9.27RR69 pKa = 11.84AALLIAVLGALFSPLGIYY87 pKa = 9.11VAHH90 pKa = 6.2QLPDD94 pKa = 3.63NVLMILFSALMVLVAWRR111 pKa = 11.84MLRR114 pKa = 11.84RR115 pKa = 11.84EE116 pKa = 4.02QPAPGPSDD124 pKa = 3.38HH125 pKa = 6.83GQANWGQKK133 pKa = 9.73NCMLDD138 pKa = 3.29QQTGRR143 pKa = 11.84FSWTAKK149 pKa = 8.94CTATLAGLGAVTGVVSGLLGVGGGFLIVPAFKK181 pKa = 10.63QLTDD185 pKa = 3.22VQMRR189 pKa = 11.84GIVATSLMVISLISAIGVLGAFHH212 pKa = 7.06AGVRR216 pKa = 11.84IDD218 pKa = 3.62SQGVAFIVASILGMIIGRR236 pKa = 11.84RR237 pKa = 11.84LCARR241 pKa = 11.84VPARR245 pKa = 11.84ALQMGFASVCLVVAGYY261 pKa = 8.7MLLKK265 pKa = 10.73AA266 pKa = 4.78

Molecular weight: 27.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4728

0

4728

1540914

39

3116

325.9

35.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.884 ± 0.039

0.993 ± 0.01

5.378 ± 0.027

5.503 ± 0.036

3.634 ± 0.019

7.886 ± 0.038

2.352 ± 0.019

4.859 ± 0.027

3.573 ± 0.03

11.77 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.357 ± 0.017

3.098 ± 0.03

4.908 ± 0.026

4.613 ± 0.026

6.219 ± 0.035

5.79 ± 0.026

5.012 ± 0.034

7.163 ± 0.032

1.433 ± 0.016

2.572 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski