Amino acid dipepetide frequency for Pseudomonas sp. ok602

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.095AlaAla: 12.095 ± 0.14
1.194AlaCys: 1.194 ± 0.034
5.856AlaAsp: 5.856 ± 0.067
6.251AlaGlu: 6.251 ± 0.068
3.774AlaPhe: 3.774 ± 0.051
8.683AlaGly: 8.683 ± 0.091
2.323AlaHis: 2.323 ± 0.04
5.26AlaIle: 5.26 ± 0.064
3.984AlaLys: 3.984 ± 0.075
13.653AlaLeu: 13.653 ± 0.124
2.928AlaMet: 2.928 ± 0.049
3.133AlaAsn: 3.133 ± 0.052
4.855AlaPro: 4.855 ± 0.074
5.513AlaGln: 5.513 ± 0.073
6.639AlaArg: 6.639 ± 0.077
6.146AlaSer: 6.146 ± 0.064
5.047AlaThr: 5.047 ± 0.067
7.356AlaVal: 7.356 ± 0.077
1.546AlaTrp: 1.546 ± 0.033
2.608AlaTyr: 2.608 ± 0.041
0.0AlaXaa: 0.0 ± 0.0
Cys
1.101CysAla: 1.101 ± 0.032
0.135CysCys: 0.135 ± 0.011
0.54CysAsp: 0.54 ± 0.02
0.543CysGlu: 0.543 ± 0.02
0.332CysPhe: 0.332 ± 0.014
0.951CysGly: 0.951 ± 0.026
0.296CysHis: 0.296 ± 0.016
0.43CysIle: 0.43 ± 0.018
0.286CysLys: 0.286 ± 0.012
1.13CysLeu: 1.13 ± 0.029
0.199CysMet: 0.199 ± 0.01
0.274CysAsn: 0.274 ± 0.015
0.486CysPro: 0.486 ± 0.019
0.4CysGln: 0.4 ± 0.016
0.597CysArg: 0.597 ± 0.021
0.587CysSer: 0.587 ± 0.021
0.516CysThr: 0.516 ± 0.018
0.722CysVal: 0.722 ± 0.022
0.164CysTrp: 0.164 ± 0.01
0.239CysTyr: 0.239 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
5.627AspAla: 5.627 ± 0.058
0.552AspCys: 0.552 ± 0.02
2.863AspAsp: 2.863 ± 0.043
3.376AspGlu: 3.376 ± 0.047
2.037AspPhe: 2.037 ± 0.04
4.403AspGly: 4.403 ± 0.063
1.201AspHis: 1.201 ± 0.026
2.696AspIle: 2.696 ± 0.042
2.024AspLys: 2.024 ± 0.043
5.915AspLeu: 5.915 ± 0.075
1.26AspMet: 1.26 ± 0.029
1.701AspAsn: 1.701 ± 0.037
2.907AspPro: 2.907 ± 0.047
2.279AspGln: 2.279 ± 0.044
2.955AspArg: 2.955 ± 0.05
2.984AspSer: 2.984 ± 0.045
2.625AspThr: 2.625 ± 0.047
3.682AspVal: 3.682 ± 0.053
0.935AspTrp: 0.935 ± 0.026
1.758AspTyr: 1.758 ± 0.035
0.0AspXaa: 0.0 ± 0.0
Glu
5.969GluAla: 5.969 ± 0.067
0.444GluCys: 0.444 ± 0.02
2.43GluAsp: 2.43 ± 0.038
2.798GluGlu: 2.798 ± 0.057
1.841GluPhe: 1.841 ± 0.035
3.803GluGly: 3.803 ± 0.053
1.808GluHis: 1.808 ± 0.043
2.639GluIle: 2.639 ± 0.044
2.038GluLys: 2.038 ± 0.04
6.759GluLeu: 6.759 ± 0.073
1.334GluMet: 1.334 ± 0.031
1.445GluAsn: 1.445 ± 0.026
2.543GluPro: 2.543 ± 0.048
3.628GluGln: 3.628 ± 0.056
4.517GluArg: 4.517 ± 0.061
2.655GluSer: 2.655 ± 0.046
2.465GluThr: 2.465 ± 0.044
4.057GluVal: 4.057 ± 0.062
0.69GluTrp: 0.69 ± 0.023
1.162GluTyr: 1.162 ± 0.029
0.0GluXaa: 0.0 ± 0.0
Phe
3.987PheAla: 3.987 ± 0.05
0.445PheCys: 0.445 ± 0.017
2.424PheAsp: 2.424 ± 0.039
2.109PheGlu: 2.109 ± 0.04
1.467PhePhe: 1.467 ± 0.036
3.156PheGly: 3.156 ± 0.057
0.726PheHis: 0.726 ± 0.019
1.963PheIle: 1.963 ± 0.038
1.404PheLys: 1.404 ± 0.03
3.175PheLeu: 3.175 ± 0.055
0.843PheMet: 0.843 ± 0.028
1.432PheAsn: 1.432 ± 0.031
1.493PhePro: 1.493 ± 0.03
1.214PheGln: 1.214 ± 0.027
1.746PheArg: 1.746 ± 0.039
2.583PheSer: 2.583 ± 0.046
1.942PheThr: 1.942 ± 0.032
2.545PheVal: 2.545 ± 0.04
0.574PheTrp: 0.574 ± 0.024
1.116PheTyr: 1.116 ± 0.029
0.0PheXaa: 0.0 ± 0.0
Gly
7.339GlyAla: 7.339 ± 0.086
0.938GlyCys: 0.938 ± 0.027
3.967GlyAsp: 3.967 ± 0.056
4.517GlyGlu: 4.517 ± 0.054
3.353GlyPhe: 3.353 ± 0.053
6.013GlyGly: 6.013 ± 0.109
1.896GlyHis: 1.896 ± 0.04
4.105GlyIle: 4.105 ± 0.058
3.479GlyLys: 3.479 ± 0.052
9.282GlyLeu: 9.282 ± 0.081
2.262GlyMet: 2.262 ± 0.04
2.429GlyAsn: 2.429 ± 0.055
2.707GlyPro: 2.707 ± 0.042
3.735GlyGln: 3.735 ± 0.051
4.55GlyArg: 4.55 ± 0.056
4.534GlySer: 4.534 ± 0.077
3.92GlyThr: 3.92 ± 0.078
6.041GlyVal: 6.041 ± 0.069
1.376GlyTrp: 1.376 ± 0.035
2.421GlyTyr: 2.421 ± 0.037
0.0GlyXaa: 0.0 ± 0.0
His
2.371HisAla: 2.371 ± 0.043
0.327HisCys: 0.327 ± 0.015
1.236HisAsp: 1.236 ± 0.031
1.207HisGlu: 1.207 ± 0.029
1.013HisPhe: 1.013 ± 0.028
1.994HisGly: 1.994 ± 0.035
0.655HisHis: 0.655 ± 0.023
1.042HisIle: 1.042 ± 0.027
0.709HisLys: 0.709 ± 0.021
2.832HisLeu: 2.832 ± 0.048
0.527HisMet: 0.527 ± 0.02
0.692HisAsn: 0.692 ± 0.022
1.463HisPro: 1.463 ± 0.035
0.973HisGln: 0.973 ± 0.023
1.314HisArg: 1.314 ± 0.032
1.296HisSer: 1.296 ± 0.034
1.084HisThr: 1.084 ± 0.029
1.547HisVal: 1.547 ± 0.036
0.465HisTrp: 0.465 ± 0.018
0.77HisTyr: 0.77 ± 0.024
0.0HisXaa: 0.0 ± 0.0
Ile
5.902IleAla: 5.902 ± 0.059
0.509IleCys: 0.509 ± 0.015
3.336IleAsp: 3.336 ± 0.051
3.351IleGlu: 3.351 ± 0.051
1.471IlePhe: 1.471 ± 0.036
4.534IleGly: 4.534 ± 0.066
1.024IleHis: 1.024 ± 0.026
2.18IleIle: 2.18 ± 0.041
1.935IleLys: 1.935 ± 0.039
4.083IleLeu: 4.083 ± 0.063
0.841IleMet: 0.841 ± 0.025
1.898IleAsn: 1.898 ± 0.042
2.261IlePro: 2.261 ± 0.041
1.64IleGln: 1.64 ± 0.035
2.785IleArg: 2.785 ± 0.046
3.108IleSer: 3.108 ± 0.045
2.735IleThr: 2.735 ± 0.046
3.355IleVal: 3.355 ± 0.046
0.486IleTrp: 0.486 ± 0.016
1.158IleTyr: 1.158 ± 0.026
0.0IleXaa: 0.0 ± 0.0
Lys
4.273LysAla: 4.273 ± 0.07
0.198LysCys: 0.198 ± 0.012
1.956LysAsp: 1.956 ± 0.04
1.707LysGlu: 1.707 ± 0.04
0.898LysPhe: 0.898 ± 0.026
2.736LysGly: 2.736 ± 0.045
0.89LysHis: 0.89 ± 0.024
1.724LysIle: 1.724 ± 0.039
1.359LysLys: 1.359 ± 0.041
3.856LysLeu: 3.856 ± 0.056
0.774LysMet: 0.774 ± 0.023
1.132LysAsn: 1.132 ± 0.026
2.131LysPro: 2.131 ± 0.047
1.64LysGln: 1.64 ± 0.033
2.459LysArg: 2.459 ± 0.039
1.941LysSer: 1.941 ± 0.04
2.016LysThr: 2.016 ± 0.036
2.917LysVal: 2.917 ± 0.041
0.374LysTrp: 0.374 ± 0.015
0.75LysTyr: 0.75 ± 0.024
0.0LysXaa: 0.0 ± 0.0
Leu
13.827LeuAla: 13.827 ± 0.121
1.236LeuCys: 1.236 ± 0.034
6.882LeuAsp: 6.882 ± 0.087
6.272LeuGlu: 6.272 ± 0.071
4.153LeuPhe: 4.153 ± 0.058
9.21LeuGly: 9.21 ± 0.104
2.544LeuHis: 2.544 ± 0.046
5.806LeuIle: 5.806 ± 0.07
4.704LeuLys: 4.704 ± 0.065
13.364LeuLeu: 13.364 ± 0.144
2.652LeuMet: 2.652 ± 0.045
3.918LeuAsn: 3.918 ± 0.077
6.185LeuPro: 6.185 ± 0.075
4.578LeuGln: 4.578 ± 0.072
7.12LeuArg: 7.12 ± 0.084
7.488LeuSer: 7.488 ± 0.081
6.007LeuThr: 6.007 ± 0.08
7.751LeuVal: 7.751 ± 0.085
1.391LeuTrp: 1.391 ± 0.036
2.614LeuTyr: 2.614 ± 0.043
0.0LeuXaa: 0.0 ± 0.0
Met
2.669MetAla: 2.669 ± 0.04
0.184MetCys: 0.184 ± 0.011
1.042MetAsp: 1.042 ± 0.033
0.962MetGlu: 0.962 ± 0.025
0.735MetPhe: 0.735 ± 0.021
1.767MetGly: 1.767 ± 0.037
0.504MetHis: 0.504 ± 0.019
1.217MetIle: 1.217 ± 0.031
0.893MetLys: 0.893 ± 0.028
2.752MetLeu: 2.752 ± 0.046
0.523MetMet: 0.523 ± 0.021
0.896MetAsn: 0.896 ± 0.024
1.385MetPro: 1.385 ± 0.031
1.016MetGln: 1.016 ± 0.025
1.471MetArg: 1.471 ± 0.032
1.807MetSer: 1.807 ± 0.035
1.517MetThr: 1.517 ± 0.034
1.641MetVal: 1.641 ± 0.036
0.193MetTrp: 0.193 ± 0.012
0.396MetTyr: 0.396 ± 0.016
0.0MetXaa: 0.0 ± 0.0
Asn
3.336AsnAla: 3.336 ± 0.059
0.294AsnCys: 0.294 ± 0.014
1.668AsnAsp: 1.668 ± 0.039
1.509AsnGlu: 1.509 ± 0.031
1.023AsnPhe: 1.023 ± 0.03
2.724AsnGly: 2.724 ± 0.059
0.674AsnHis: 0.674 ± 0.021
1.48AsnIle: 1.48 ± 0.037
0.999AsnLys: 0.999 ± 0.027
3.522AsnLeu: 3.522 ± 0.063
0.6AsnMet: 0.6 ± 0.02
1.04AsnAsn: 1.04 ± 0.039
2.086AsnPro: 2.086 ± 0.037
1.383AsnGln: 1.383 ± 0.031
1.894AsnArg: 1.894 ± 0.033
1.624AsnSer: 1.624 ± 0.037
1.644AsnThr: 1.644 ± 0.044
2.125AsnVal: 2.125 ± 0.045
0.488AsnTrp: 0.488 ± 0.016
0.87AsnTyr: 0.87 ± 0.025
0.0AsnXaa: 0.0 ± 0.0
Pro
5.711ProAla: 5.711 ± 0.077
0.373ProCys: 0.373 ± 0.017
2.873ProAsp: 2.873 ± 0.046
3.095ProGlu: 3.095 ± 0.038
1.784ProPhe: 1.784 ± 0.037
3.913ProGly: 3.913 ± 0.051
1.134ProHis: 1.134 ± 0.033
2.136ProIle: 2.136 ± 0.033
1.694ProLys: 1.694 ± 0.036
5.701ProLeu: 5.701 ± 0.071
1.224ProMet: 1.224 ± 0.029
1.463ProAsn: 1.463 ± 0.034
2.018ProPro: 2.018 ± 0.046
2.417ProGln: 2.417 ± 0.042
2.535ProArg: 2.535 ± 0.039
2.7ProSer: 2.7 ± 0.043
2.431ProThr: 2.431 ± 0.041
3.873ProVal: 3.873 ± 0.054
0.76ProTrp: 0.76 ± 0.024
1.25ProTyr: 1.25 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
5.957GlnAla: 5.957 ± 0.079
0.334GlnCys: 0.334 ± 0.013
1.935GlnAsp: 1.935 ± 0.038
1.809GlnGlu: 1.809 ± 0.038
1.412GlnPhe: 1.412 ± 0.031
3.465GlnGly: 3.465 ± 0.052
1.244GlnHis: 1.244 ± 0.035
2.112GlnIle: 2.112 ± 0.037
1.298GlnLys: 1.298 ± 0.032
5.669GlnLeu: 5.669 ± 0.076
1.092GlnMet: 1.092 ± 0.029
1.088GlnAsn: 1.088 ± 0.025
2.431GlnPro: 2.431 ± 0.046
2.728GlnGln: 2.728 ± 0.065
3.694GlnArg: 3.694 ± 0.059
2.297GlnSer: 2.297 ± 0.044
2.069GlnThr: 2.069 ± 0.044
3.668GlnVal: 3.668 ± 0.055
0.783GlnTrp: 0.783 ± 0.024
1.045GlnTyr: 1.045 ± 0.025
0.0GlnXaa: 0.0 ± 0.0
Arg
5.856ArgAla: 5.856 ± 0.071
0.604ArgCys: 0.604 ± 0.021
3.36ArgAsp: 3.36 ± 0.051
4.028ArgGlu: 4.028 ± 0.06
2.609ArgPhe: 2.609 ± 0.045
3.894ArgGly: 3.894 ± 0.052
1.649ArgHis: 1.649 ± 0.037
3.22ArgIle: 3.22 ± 0.041
2.034ArgLys: 2.034 ± 0.039
7.976ArgLeu: 7.976 ± 0.091
1.556ArgMet: 1.556 ± 0.03
1.9ArgAsn: 1.9 ± 0.034
2.694ArgPro: 2.694 ± 0.046
3.281ArgGln: 3.281 ± 0.049
3.976ArgArg: 3.976 ± 0.055
3.274ArgSer: 3.274 ± 0.038
2.771ArgThr: 2.771 ± 0.044
4.458ArgVal: 4.458 ± 0.058
1.029ArgTrp: 1.029 ± 0.024
2.023ArgTyr: 2.023 ± 0.036
0.0ArgXaa: 0.0 ± 0.0
Ser
6.189SerAla: 6.189 ± 0.071
0.471SerCys: 0.471 ± 0.018
2.915SerAsp: 2.915 ± 0.044
3.035SerGlu: 3.035 ± 0.048
2.203SerPhe: 2.203 ± 0.038
5.107SerGly: 5.107 ± 0.079
1.353SerHis: 1.353 ± 0.03
2.841SerIle: 2.841 ± 0.046
1.913SerLys: 1.913 ± 0.041
7.128SerLeu: 7.128 ± 0.068
1.442SerMet: 1.442 ± 0.029
1.794SerAsn: 1.794 ± 0.045
2.749SerPro: 2.749 ± 0.039
2.445SerGln: 2.445 ± 0.039
3.533SerArg: 3.533 ± 0.045
3.42SerSer: 3.42 ± 0.061
3.037SerThr: 3.037 ± 0.051
4.118SerVal: 4.118 ± 0.053
0.77SerTrp: 0.77 ± 0.023
1.438SerTyr: 1.438 ± 0.034
0.0SerXaa: 0.0 ± 0.0
Thr
5.287ThrAla: 5.287 ± 0.059
0.495ThrCys: 0.495 ± 0.019
2.524ThrAsp: 2.524 ± 0.042
2.389ThrGlu: 2.389 ± 0.043
1.962ThrPhe: 1.962 ± 0.036
4.186ThrGly: 4.186 ± 0.064
1.177ThrHis: 1.177 ± 0.026
2.081ThrIle: 2.081 ± 0.043
1.184ThrLys: 1.184 ± 0.026
7.027ThrLeu: 7.027 ± 0.097
0.855ThrMet: 0.855 ± 0.023
1.234ThrAsn: 1.234 ± 0.033
3.258ThrPro: 3.258 ± 0.05
2.259ThrGln: 2.259 ± 0.039
3.126ThrArg: 3.126 ± 0.048
2.693ThrSer: 2.693 ± 0.045
2.544ThrThr: 2.544 ± 0.043
3.728ThrVal: 3.728 ± 0.057
0.768ThrTrp: 0.768 ± 0.024
1.344ThrTyr: 1.344 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
7.594ValAla: 7.594 ± 0.076
0.737ValCys: 0.737 ± 0.022
3.972ValAsp: 3.972 ± 0.052
4.286ValGlu: 4.286 ± 0.057
2.717ValPhe: 2.717 ± 0.046
5.301ValGly: 5.301 ± 0.067
1.466ValHis: 1.466 ± 0.035
3.954ValIle: 3.954 ± 0.06
2.505ValLys: 2.505 ± 0.043
8.475ValLeu: 8.475 ± 0.089
1.807ValMet: 1.807 ± 0.034
2.284ValAsn: 2.284 ± 0.048
3.404ValPro: 3.404 ± 0.042
2.789ValGln: 2.789 ± 0.047
4.26ValArg: 4.26 ± 0.054
4.402ValSer: 4.402 ± 0.06
3.759ValThr: 3.759 ± 0.059
5.411ValVal: 5.411 ± 0.071
0.856ValTrp: 0.856 ± 0.022
1.653ValTyr: 1.653 ± 0.034
0.0ValXaa: 0.0 ± 0.0
Trp
1.188TrpAla: 1.188 ± 0.03
0.167TrpCys: 0.167 ± 0.011
0.642TrpAsp: 0.642 ± 0.022
0.586TrpGlu: 0.586 ± 0.022
0.526TrpPhe: 0.526 ± 0.016
0.955TrpGly: 0.955 ± 0.032
0.368TrpHis: 0.368 ± 0.016
0.668TrpIle: 0.668 ± 0.02
0.485TrpLys: 0.485 ± 0.015
2.216TrpLeu: 2.216 ± 0.039
0.38TrpMet: 0.38 ± 0.017
0.446TrpAsn: 0.446 ± 0.017
0.701TrpPro: 0.701 ± 0.023
0.888TrpGln: 0.888 ± 0.029
1.043TrpArg: 1.043 ± 0.032
0.827TrpSer: 0.827 ± 0.025
0.658TrpThr: 0.658 ± 0.021
0.994TrpVal: 0.994 ± 0.025
0.24TrpTrp: 0.24 ± 0.012
0.351TrpTyr: 0.351 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.568TyrAla: 2.568 ± 0.041
0.289TyrCys: 0.289 ± 0.014
1.359TyrAsp: 1.359 ± 0.033
1.196TyrGlu: 1.196 ± 0.027
1.033TyrPhe: 1.033 ± 0.025
2.069TyrGly: 2.069 ± 0.039
0.586TyrHis: 0.586 ± 0.018
1.037TyrIle: 1.037 ± 0.027
0.801TyrLys: 0.801 ± 0.022
3.191TyrLeu: 3.191 ± 0.05
0.472TyrMet: 0.472 ± 0.019
0.781TyrAsn: 0.781 ± 0.026
1.328TyrPro: 1.328 ± 0.03
1.325TyrGln: 1.325 ± 0.032
1.977TyrArg: 1.977 ± 0.039
1.535TyrSer: 1.535 ± 0.031
1.334TyrThr: 1.334 ± 0.032
1.644TyrVal: 1.644 ± 0.034
0.441TyrTrp: 0.441 ± 0.019
0.755TyrTyr: 0.755 ± 0.028
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4728 proteins (1540914 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski