Microviridae Fen7940_21

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G2UG71|A0A0G2UG71_9VIRU Uncharacterized protein OS=Microviridae Fen7940_21 OX=1655662 PE=4 SV=1
MM1 pKa = 7.37EE2 pKa = 4.23ATVRR6 pKa = 11.84ILRR9 pKa = 11.84KK10 pKa = 9.21IAVFDD15 pKa = 4.32GPVDD19 pKa = 3.62ADD21 pKa = 3.84GVQVSSGQDD30 pKa = 3.08EE31 pKa = 4.63NGRR34 pKa = 11.84EE35 pKa = 4.18IPDD38 pKa = 3.76PVPFAVPTDD47 pKa = 3.81LKK49 pKa = 11.15RR50 pKa = 11.84PDD52 pKa = 3.78TLAEE56 pKa = 3.98MIKK59 pKa = 10.51RR60 pKa = 11.84MVRR63 pKa = 11.84SEE65 pKa = 4.17TLNAAIASEE74 pKa = 4.32DD75 pKa = 3.49FDD77 pKa = 4.41TFDD80 pKa = 3.56EE81 pKa = 4.89ASDD84 pKa = 3.96FDD86 pKa = 5.23VEE88 pKa = 5.56DD89 pKa = 5.3DD90 pKa = 4.34PLDD93 pKa = 3.52PHH95 pKa = 6.41TPYY98 pKa = 10.77EE99 pKa = 4.2AVFDD103 pKa = 5.5PIPDD107 pKa = 4.26PAPAAPAAAPAAPTGGCRR125 pKa = 11.84GNPPLRR131 pKa = 11.84LPLLRR136 pKa = 11.84PPLRR140 pKa = 11.84LLWCLL145 pKa = 3.46

Molecular weight: 15.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G2UK68|A0A0G2UK68_9VIRU Major capsid protein VP1 OS=Microviridae Fen7940_21 OX=1655662 PE=3 SV=1
MM1 pKa = 7.54GLFSKK6 pKa = 9.23YY7 pKa = 7.37TYY9 pKa = 11.09KK10 pKa = 10.17MPEE13 pKa = 3.95RR14 pKa = 11.84LSADD18 pKa = 3.53EE19 pKa = 3.74ARR21 pKa = 11.84QRR23 pKa = 11.84RR24 pKa = 11.84FFAEE28 pKa = 4.52LSRR31 pKa = 11.84ARR33 pKa = 11.84AEE35 pKa = 3.9LEE37 pKa = 3.7ARR39 pKa = 11.84NDD41 pKa = 3.71RR42 pKa = 11.84LSPANKK48 pKa = 9.42FHH50 pKa = 6.61LTQLKK55 pKa = 9.08EE56 pKa = 4.15FQVKK60 pKa = 10.19FSDD63 pKa = 3.49EE64 pKa = 3.84LEE66 pKa = 4.05ARR68 pKa = 11.84RR69 pKa = 11.84KK70 pKa = 9.41RR71 pKa = 11.84VRR73 pKa = 11.84DD74 pKa = 3.4VLEE77 pKa = 4.5RR78 pKa = 11.84DD79 pKa = 3.5PSISRR84 pKa = 11.84SLAYY88 pKa = 9.59KK89 pKa = 10.14SAMSRR94 pKa = 11.84LSKK97 pKa = 10.3LQQRR101 pKa = 11.84QTALLNRR108 pKa = 11.84AAAEE112 pKa = 3.95RR113 pKa = 11.84RR114 pKa = 11.84AKK116 pKa = 10.54AFSASASAADD126 pKa = 3.53RR127 pKa = 11.84RR128 pKa = 11.84SFSFTNRR135 pKa = 11.84FGNPKK140 pKa = 9.65TIYY143 pKa = 7.92TTDD146 pKa = 2.41AWIKK150 pKa = 9.66VNADD154 pKa = 2.71AVTRR158 pKa = 11.84RR159 pKa = 11.84MFRR162 pKa = 11.84SPTLSIPCIQRR173 pKa = 11.84MVRR176 pKa = 11.84RR177 pKa = 11.84EE178 pKa = 3.8VIFARR183 pKa = 11.84RR184 pKa = 11.84HH185 pKa = 4.93NKK187 pKa = 7.11GHH189 pKa = 5.81RR190 pKa = 11.84VKK192 pKa = 10.86HH193 pKa = 5.52RR194 pKa = 11.84FNPLSLIGCC203 pKa = 4.63

Molecular weight: 23.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1351

145

507

270.2

29.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.733 ± 0.794

1.11 ± 0.528

5.033 ± 0.873

4.293 ± 0.634

3.923 ± 0.59

7.402 ± 1.264

2.073 ± 0.609

3.627 ± 0.434

2.813 ± 0.725

7.994 ± 0.527

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.591 ± 0.089

4.293 ± 0.958

5.551 ± 1.209

4.367 ± 0.437

8.438 ± 1.692

8.66 ± 1.22

6.514 ± 1.077

6.218 ± 0.525

1.48 ± 0.427

2.887 ± 0.493
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski