Amino acid dipepetide frequency for Mycobacterium phage Jolie2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.108AlaAla: 17.108 ± 1.828
1.182AlaCys: 1.182 ± 0.334
8.206AlaAsp: 8.206 ± 0.942
10.015AlaGlu: 10.015 ± 1.375
4.103AlaPhe: 4.103 ± 0.483
14.535AlaGly: 14.535 ± 1.282
2.225AlaHis: 2.225 ± 0.332
7.233AlaIle: 7.233 ± 0.707
4.52AlaLys: 4.52 ± 0.488
11.475AlaLeu: 11.475 ± 1.376
2.434AlaMet: 2.434 ± 0.396
3.825AlaAsn: 3.825 ± 0.712
7.441AlaPro: 7.441 ± 0.855
4.66AlaGln: 4.66 ± 0.55
7.163AlaArg: 7.163 ± 1.047
5.772AlaSer: 5.772 ± 1.008
7.094AlaThr: 7.094 ± 0.745
8.763AlaVal: 8.763 ± 1.022
2.365AlaTrp: 2.365 ± 0.343
1.739AlaTyr: 1.739 ± 0.326
0.0AlaXaa: 0.0 ± 0.0
Cys
1.182CysAla: 1.182 ± 0.283
0.139CysCys: 0.139 ± 0.099
0.278CysAsp: 0.278 ± 0.15
0.209CysGlu: 0.209 ± 0.109
0.209CysPhe: 0.209 ± 0.122
1.182CysGly: 1.182 ± 0.357
0.139CysHis: 0.139 ± 0.091
0.417CysIle: 0.417 ± 0.169
0.139CysLys: 0.139 ± 0.099
0.417CysLeu: 0.417 ± 0.168
0.209CysMet: 0.209 ± 0.153
0.278CysAsn: 0.278 ± 0.138
0.904CysPro: 0.904 ± 0.295
0.417CysGln: 0.417 ± 0.166
0.835CysArg: 0.835 ± 0.262
0.417CysSer: 0.417 ± 0.246
0.417CysThr: 0.417 ± 0.169
0.417CysVal: 0.417 ± 0.16
0.626CysTrp: 0.626 ± 0.246
0.07CysTyr: 0.07 ± 0.061
0.0CysXaa: 0.0 ± 0.0
Asp
8.346AspAla: 8.346 ± 0.952
0.765AspCys: 0.765 ± 0.266
6.955AspAsp: 6.955 ± 0.786
5.633AspGlu: 5.633 ± 0.72
1.878AspPhe: 1.878 ± 0.418
7.789AspGly: 7.789 ± 0.834
1.739AspHis: 1.739 ± 0.286
0.904AspIle: 0.904 ± 0.309
1.53AspLys: 1.53 ± 0.316
4.034AspLeu: 4.034 ± 0.512
1.113AspMet: 1.113 ± 0.231
0.974AspAsn: 0.974 ± 0.306
5.425AspPro: 5.425 ± 0.767
4.103AspGln: 4.103 ± 0.663
5.494AspArg: 5.494 ± 0.716
2.017AspSer: 2.017 ± 0.344
3.616AspThr: 3.616 ± 0.496
6.19AspVal: 6.19 ± 0.656
1.46AspTrp: 1.46 ± 0.299
2.086AspTyr: 2.086 ± 0.347
0.0AspXaa: 0.0 ± 0.0
Glu
8.346GluAla: 8.346 ± 1.107
0.417GluCys: 0.417 ± 0.153
4.103GluAsp: 4.103 ± 0.566
2.573GluGlu: 2.573 ± 0.541
2.086GluPhe: 2.086 ± 0.39
3.477GluGly: 3.477 ± 0.472
2.295GluHis: 2.295 ± 0.377
1.739GluIle: 1.739 ± 0.31
3.13GluLys: 3.13 ± 0.47
4.173GluLeu: 4.173 ± 0.558
1.182GluMet: 1.182 ± 0.352
2.295GluAsn: 2.295 ± 0.481
4.451GluPro: 4.451 ± 0.78
1.252GluGln: 1.252 ± 0.271
4.59GluArg: 4.59 ± 0.812
2.225GluSer: 2.225 ± 0.422
2.086GluThr: 2.086 ± 0.388
3.408GluVal: 3.408 ± 0.48
1.46GluTrp: 1.46 ± 0.328
1.182GluTyr: 1.182 ± 0.293
0.0GluXaa: 0.0 ± 0.0
Phe
4.312PheAla: 4.312 ± 0.559
0.348PheCys: 0.348 ± 0.129
2.851PheAsp: 2.851 ± 0.39
2.434PheGlu: 2.434 ± 0.39
0.904PhePhe: 0.904 ± 0.208
3.616PheGly: 3.616 ± 0.528
0.139PheHis: 0.139 ± 0.088
1.043PheIle: 1.043 ± 0.196
1.182PheLys: 1.182 ± 0.274
1.53PheLeu: 1.53 ± 0.303
0.417PheMet: 0.417 ± 0.152
0.904PheAsn: 0.904 ± 0.216
1.46PhePro: 1.46 ± 0.346
0.556PheGln: 0.556 ± 0.174
1.113PheArg: 1.113 ± 0.217
1.043PheSer: 1.043 ± 0.273
2.225PheThr: 2.225 ± 0.374
2.434PheVal: 2.434 ± 0.455
0.417PheTrp: 0.417 ± 0.17
0.487PheTyr: 0.487 ± 0.182
0.0PheXaa: 0.0 ± 0.0
Gly
10.571GlyAla: 10.571 ± 1.441
0.765GlyCys: 0.765 ± 0.2
7.511GlyAsp: 7.511 ± 0.682
4.52GlyGlu: 4.52 ± 0.464
3.338GlyPhe: 3.338 ± 0.49
9.945GlyGly: 9.945 ± 2.453
1.878GlyHis: 1.878 ± 0.383
4.034GlyIle: 4.034 ± 0.541
3.547GlyLys: 3.547 ± 0.719
6.468GlyLeu: 6.468 ± 0.777
1.808GlyMet: 1.808 ± 0.332
3.895GlyAsn: 3.895 ± 0.692
4.729GlyPro: 4.729 ± 0.561
2.712GlyGln: 2.712 ± 0.445
5.842GlyArg: 5.842 ± 0.595
5.425GlySer: 5.425 ± 0.81
5.703GlyThr: 5.703 ± 0.615
6.259GlyVal: 6.259 ± 0.856
2.504GlyTrp: 2.504 ± 0.335
1.878GlyTyr: 1.878 ± 0.334
0.0GlyXaa: 0.0 ± 0.0
His
2.782HisAla: 2.782 ± 0.521
0.278HisCys: 0.278 ± 0.143
1.182HisAsp: 1.182 ± 0.348
0.835HisGlu: 0.835 ± 0.232
0.626HisPhe: 0.626 ± 0.237
2.017HisGly: 2.017 ± 0.308
0.348HisHis: 0.348 ± 0.149
0.974HisIle: 0.974 ± 0.23
0.417HisLys: 0.417 ± 0.164
1.043HisLeu: 1.043 ± 0.243
0.417HisMet: 0.417 ± 0.156
0.417HisAsn: 0.417 ± 0.183
1.808HisPro: 1.808 ± 0.384
0.904HisGln: 0.904 ± 0.25
2.504HisArg: 2.504 ± 0.503
0.417HisSer: 0.417 ± 0.162
1.391HisThr: 1.391 ± 0.277
1.947HisVal: 1.947 ± 0.324
0.07HisTrp: 0.07 ± 0.061
0.209HisTyr: 0.209 ± 0.107
0.0HisXaa: 0.0 ± 0.0
Ile
7.233IleAla: 7.233 ± 0.613
0.209IleCys: 0.209 ± 0.119
3.338IleAsp: 3.338 ± 0.466
3.338IleGlu: 3.338 ± 0.435
0.835IlePhe: 0.835 ± 0.2
4.938IleGly: 4.938 ± 1.084
0.695IleHis: 0.695 ± 0.206
0.835IleIle: 0.835 ± 0.229
1.043IleLys: 1.043 ± 0.245
2.643IleLeu: 2.643 ± 0.512
0.904IleMet: 0.904 ± 0.271
0.765IleAsn: 0.765 ± 0.192
3.616IlePro: 3.616 ± 0.461
0.835IleGln: 0.835 ± 0.168
2.782IleArg: 2.782 ± 0.37
1.46IleSer: 1.46 ± 0.299
3.199IleThr: 3.199 ± 0.391
3.408IleVal: 3.408 ± 0.424
1.043IleTrp: 1.043 ± 0.236
0.904IleTyr: 0.904 ± 0.245
0.0IleXaa: 0.0 ± 0.0
Lys
5.564LysAla: 5.564 ± 0.796
0.139LysCys: 0.139 ± 0.107
1.46LysAsp: 1.46 ± 0.376
0.974LysGlu: 0.974 ± 0.222
0.974LysPhe: 0.974 ± 0.199
1.808LysGly: 1.808 ± 0.313
0.904LysHis: 0.904 ± 0.227
1.669LysIle: 1.669 ± 0.314
1.113LysLys: 1.113 ± 0.358
3.06LysLeu: 3.06 ± 0.436
1.043LysMet: 1.043 ± 0.203
1.46LysAsn: 1.46 ± 0.295
2.504LysPro: 2.504 ± 0.41
0.974LysGln: 0.974 ± 0.213
3.895LysArg: 3.895 ± 0.513
2.086LysSer: 2.086 ± 0.386
2.712LysThr: 2.712 ± 0.431
2.225LysVal: 2.225 ± 0.343
0.487LysTrp: 0.487 ± 0.152
0.695LysTyr: 0.695 ± 0.258
0.0LysXaa: 0.0 ± 0.0
Leu
9.806LeuAla: 9.806 ± 0.996
0.348LeuCys: 0.348 ± 0.185
7.163LeuAsp: 7.163 ± 0.666
3.686LeuGlu: 3.686 ± 0.426
2.365LeuPhe: 2.365 ± 0.417
6.19LeuGly: 6.19 ± 0.901
1.321LeuHis: 1.321 ± 0.269
3.686LeuIle: 3.686 ± 0.438
2.086LeuLys: 2.086 ± 0.295
5.703LeuLeu: 5.703 ± 0.566
1.739LeuMet: 1.739 ± 0.335
1.808LeuAsn: 1.808 ± 0.34
4.66LeuPro: 4.66 ± 0.765
1.182LeuGln: 1.182 ± 0.247
5.007LeuArg: 5.007 ± 0.619
4.938LeuSer: 4.938 ± 0.515
6.12LeuThr: 6.12 ± 0.69
4.868LeuVal: 4.868 ± 0.463
1.252LeuTrp: 1.252 ± 0.343
1.947LeuTyr: 1.947 ± 0.46
0.0LeuXaa: 0.0 ± 0.0
Met
2.712MetAla: 2.712 ± 0.37
0.278MetCys: 0.278 ± 0.135
0.974MetAsp: 0.974 ± 0.212
0.765MetGlu: 0.765 ± 0.227
0.765MetPhe: 0.765 ± 0.201
1.321MetGly: 1.321 ± 0.278
0.417MetHis: 0.417 ± 0.158
1.391MetIle: 1.391 ± 0.317
0.556MetLys: 0.556 ± 0.233
1.53MetLeu: 1.53 ± 0.254
0.487MetMet: 0.487 ± 0.197
0.765MetAsn: 0.765 ± 0.216
1.043MetPro: 1.043 ± 0.263
0.835MetGln: 0.835 ± 0.249
1.252MetArg: 1.252 ± 0.279
2.017MetSer: 2.017 ± 0.374
1.46MetThr: 1.46 ± 0.258
1.46MetVal: 1.46 ± 0.265
0.278MetTrp: 0.278 ± 0.129
0.626MetTyr: 0.626 ± 0.208
0.0MetXaa: 0.0 ± 0.0
Asn
3.825AsnAla: 3.825 ± 0.432
0.626AsnCys: 0.626 ± 0.212
2.156AsnAsp: 2.156 ± 0.39
1.53AsnGlu: 1.53 ± 0.4
0.835AsnPhe: 0.835 ± 0.22
3.755AsnGly: 3.755 ± 0.709
0.626AsnHis: 0.626 ± 0.197
0.626AsnIle: 0.626 ± 0.181
0.835AsnLys: 0.835 ± 0.208
1.53AsnLeu: 1.53 ± 0.273
0.626AsnMet: 0.626 ± 0.22
0.417AsnAsn: 0.417 ± 0.148
3.408AsnPro: 3.408 ± 0.461
1.113AsnGln: 1.113 ± 0.234
1.878AsnArg: 1.878 ± 0.315
0.348AsnSer: 0.348 ± 0.14
1.53AsnThr: 1.53 ± 0.302
1.947AsnVal: 1.947 ± 0.551
0.348AsnTrp: 0.348 ± 0.137
0.765AsnTyr: 0.765 ± 0.211
0.0AsnXaa: 0.0 ± 0.0
Pro
11.336ProAla: 11.336 ± 1.079
0.417ProCys: 0.417 ± 0.193
5.146ProAsp: 5.146 ± 0.645
3.686ProGlu: 3.686 ± 0.702
1.182ProPhe: 1.182 ± 0.281
4.938ProGly: 4.938 ± 0.757
1.46ProHis: 1.46 ± 0.322
3.477ProIle: 3.477 ± 0.389
2.99ProLys: 2.99 ± 0.5
4.381ProLeu: 4.381 ± 0.582
1.043ProMet: 1.043 ± 0.303
1.46ProAsn: 1.46 ± 0.369
2.851ProPro: 2.851 ± 0.588
1.808ProGln: 1.808 ± 0.409
3.269ProArg: 3.269 ± 0.581
3.547ProSer: 3.547 ± 0.41
4.034ProThr: 4.034 ± 0.496
3.964ProVal: 3.964 ± 0.588
1.043ProTrp: 1.043 ± 0.347
1.46ProTyr: 1.46 ± 0.353
0.0ProXaa: 0.0 ± 0.0
Gln
4.729GlnAla: 4.729 ± 0.499
0.209GlnCys: 0.209 ± 0.126
1.669GlnAsp: 1.669 ± 0.419
1.043GlnGlu: 1.043 ± 0.233
1.182GlnPhe: 1.182 ± 0.315
2.573GlnGly: 2.573 ± 0.394
0.974GlnHis: 0.974 ± 0.335
2.086GlnIle: 2.086 ± 0.28
1.182GlnLys: 1.182 ± 0.334
3.199GlnLeu: 3.199 ± 0.394
0.835GlnMet: 0.835 ± 0.212
1.182GlnAsn: 1.182 ± 0.339
1.739GlnPro: 1.739 ± 0.304
0.556GlnGln: 0.556 ± 0.196
2.156GlnArg: 2.156 ± 0.381
1.043GlnSer: 1.043 ± 0.237
2.156GlnThr: 2.156 ± 0.462
1.6GlnVal: 1.6 ± 0.453
0.695GlnTrp: 0.695 ± 0.218
0.417GlnTyr: 0.417 ± 0.161
0.0GlnXaa: 0.0 ± 0.0
Arg
9.18ArgAla: 9.18 ± 1.044
1.043ArgCys: 1.043 ± 0.336
4.938ArgAsp: 4.938 ± 0.593
3.825ArgGlu: 3.825 ± 0.653
1.46ArgPhe: 1.46 ± 0.321
5.077ArgGly: 5.077 ± 0.677
1.321ArgHis: 1.321 ± 0.344
2.851ArgIle: 2.851 ± 0.431
3.408ArgLys: 3.408 ± 0.616
6.398ArgLeu: 6.398 ± 0.736
1.808ArgMet: 1.808 ± 0.324
1.878ArgAsn: 1.878 ± 0.378
4.59ArgPro: 4.59 ± 0.678
2.156ArgGln: 2.156 ± 0.288
6.676ArgArg: 6.676 ± 1.003
2.712ArgSer: 2.712 ± 0.401
3.964ArgThr: 3.964 ± 0.566
2.782ArgVal: 2.782 ± 0.287
0.974ArgTrp: 0.974 ± 0.273
1.043ArgTyr: 1.043 ± 0.287
0.0ArgXaa: 0.0 ± 0.0
Ser
4.938SerAla: 4.938 ± 0.695
0.348SerCys: 0.348 ± 0.167
3.616SerAsp: 3.616 ± 0.463
2.365SerGlu: 2.365 ± 0.471
1.53SerPhe: 1.53 ± 0.287
4.59SerGly: 4.59 ± 0.644
0.695SerHis: 0.695 ± 0.257
2.99SerIle: 2.99 ± 0.581
2.434SerLys: 2.434 ± 0.394
3.547SerLeu: 3.547 ± 0.636
1.669SerMet: 1.669 ± 0.328
1.46SerAsn: 1.46 ± 0.269
2.086SerPro: 2.086 ± 0.325
1.252SerGln: 1.252 ± 0.28
2.712SerArg: 2.712 ± 0.369
2.851SerSer: 2.851 ± 0.524
3.547SerThr: 3.547 ± 0.549
2.504SerVal: 2.504 ± 0.433
0.835SerTrp: 0.835 ± 0.221
1.043SerTyr: 1.043 ± 0.291
0.0SerXaa: 0.0 ± 0.0
Thr
7.302ThrAla: 7.302 ± 0.83
0.417ThrCys: 0.417 ± 0.176
3.964ThrAsp: 3.964 ± 0.71
3.06ThrGlu: 3.06 ± 0.382
1.808ThrPhe: 1.808 ± 0.355
6.05ThrGly: 6.05 ± 0.721
1.043ThrHis: 1.043 ± 0.28
3.13ThrIle: 3.13 ± 0.43
1.947ThrLys: 1.947 ± 0.436
4.868ThrLeu: 4.868 ± 0.561
1.252ThrMet: 1.252 ± 0.364
1.878ThrAsn: 1.878 ± 0.335
4.868ThrPro: 4.868 ± 0.601
1.182ThrGln: 1.182 ± 0.307
3.547ThrArg: 3.547 ± 0.617
3.686ThrSer: 3.686 ± 0.536
4.59ThrThr: 4.59 ± 0.893
5.494ThrVal: 5.494 ± 0.548
0.974ThrTrp: 0.974 ± 0.333
1.53ThrTyr: 1.53 ± 0.282
0.0ThrXaa: 0.0 ± 0.0
Val
7.859ValAla: 7.859 ± 0.958
0.695ValCys: 0.695 ± 0.236
4.242ValAsp: 4.242 ± 0.478
4.381ValGlu: 4.381 ± 0.492
2.573ValPhe: 2.573 ± 0.423
6.537ValGly: 6.537 ± 0.696
1.043ValHis: 1.043 ± 0.28
3.408ValIle: 3.408 ± 0.422
2.643ValLys: 2.643 ± 0.431
5.494ValLeu: 5.494 ± 0.531
1.46ValMet: 1.46 ± 0.386
1.6ValAsn: 1.6 ± 0.306
3.616ValPro: 3.616 ± 0.485
2.921ValGln: 2.921 ± 0.491
4.729ValArg: 4.729 ± 0.515
2.295ValSer: 2.295 ± 0.335
4.173ValThr: 4.173 ± 0.456
5.911ValVal: 5.911 ± 0.825
2.017ValTrp: 2.017 ± 0.421
1.739ValTyr: 1.739 ± 0.387
0.0ValXaa: 0.0 ± 0.0
Trp
2.712TrpAla: 2.712 ± 0.473
0.139TrpCys: 0.139 ± 0.089
0.974TrpAsp: 0.974 ± 0.264
0.556TrpGlu: 0.556 ± 0.18
0.626TrpPhe: 0.626 ± 0.251
1.252TrpGly: 1.252 ± 0.25
0.556TrpHis: 0.556 ± 0.173
0.695TrpIle: 0.695 ± 0.19
0.487TrpLys: 0.487 ± 0.172
2.504TrpLeu: 2.504 ± 0.495
0.278TrpMet: 0.278 ± 0.15
0.974TrpAsn: 0.974 ± 0.275
1.321TrpPro: 1.321 ± 0.297
0.904TrpGln: 0.904 ± 0.225
1.321TrpArg: 1.321 ± 0.316
1.182TrpSer: 1.182 ± 0.266
1.182TrpThr: 1.182 ± 0.272
0.974TrpVal: 0.974 ± 0.264
0.278TrpTrp: 0.278 ± 0.141
0.626TrpTyr: 0.626 ± 0.212
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.017TyrAla: 2.017 ± 0.381
0.139TyrCys: 0.139 ± 0.085
1.321TyrAsp: 1.321 ± 0.29
1.043TyrGlu: 1.043 ± 0.296
0.139TyrPhe: 0.139 ± 0.102
1.739TyrGly: 1.739 ± 0.338
0.765TyrHis: 0.765 ± 0.244
0.835TyrIle: 0.835 ± 0.281
0.556TyrLys: 0.556 ± 0.163
1.878TyrLeu: 1.878 ± 0.357
0.139TyrMet: 0.139 ± 0.093
0.487TyrAsn: 0.487 ± 0.164
0.974TyrPro: 0.974 ± 0.239
0.904TyrGln: 0.904 ± 0.203
1.321TyrArg: 1.321 ± 0.276
1.53TyrSer: 1.53 ± 0.365
1.391TyrThr: 1.391 ± 0.29
2.782TyrVal: 2.782 ± 0.46
0.487TyrTrp: 0.487 ± 0.163
0.348TyrTyr: 0.348 ± 0.166
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (14380 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski