Paenibacillus pini JCM 16418

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; Paenibacillus pini

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4874 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W7Z1R7|W7Z1R7_9BACL Methyltransferase OS=Paenibacillus pini JCM 16418 OX=1236976 GN=JCM16418_2408 PE=4 SV=1
MM1 pKa = 7.1YY2 pKa = 10.04VVCKK6 pKa = 10.56DD7 pKa = 3.58HH8 pKa = 7.48VEE10 pKa = 3.96LAIDD14 pKa = 3.47MFVDD18 pKa = 3.92EE19 pKa = 5.98FEE21 pKa = 5.17DD22 pKa = 4.42APDD25 pKa = 3.59MVDD28 pKa = 3.34LKK30 pKa = 10.38EE31 pKa = 4.51TEE33 pKa = 4.12FSDD36 pKa = 2.87WDD38 pKa = 3.93PPVKK42 pKa = 10.08CAHH45 pKa = 6.67CEE47 pKa = 3.6QAGMYY52 pKa = 10.28LVVV55 pKa = 3.85

Molecular weight: 6.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W7YBY9|W7YBY9_9BACL Acetolactate synthase large subunit OS=Paenibacillus pini JCM 16418 OX=1236976 GN=JCM16418_2454 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.82PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.95KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.02NGRR28 pKa = 11.84NVLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.61GRR39 pKa = 11.84KK40 pKa = 8.87KK41 pKa = 10.59LSAA44 pKa = 3.95

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4874

0

4874

1403787

37

3455

288.0

32.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.3 ± 0.036

0.775 ± 0.011

5.234 ± 0.024

6.722 ± 0.038

4.052 ± 0.023

7.04 ± 0.032

2.19 ± 0.02

7.338 ± 0.038

5.904 ± 0.034

9.769 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.045 ± 0.018

4.288 ± 0.031

3.803 ± 0.022

4.077 ± 0.026

4.473 ± 0.03

6.743 ± 0.031

5.586 ± 0.036

6.993 ± 0.032

1.185 ± 0.013

3.483 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski