Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum; Methanocorpusculum labreanum

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1739 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|A2STJ3|RNP4_METLZ Ribonuclease P protein component 4 OS=Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z) OX=410358 GN=rnp4 PE=3 SV=1
MM1 pKa = 7.6ISNGVVQKK9 pKa = 9.24TLILGGILLLLITAMIVPVSASSEE33 pKa = 4.2NYY35 pKa = 9.96LVFYY39 pKa = 10.18MVGSDD44 pKa = 3.7LEE46 pKa = 4.38SGEE49 pKa = 4.47DD50 pKa = 3.51HH51 pKa = 6.64LASMNLNDD59 pKa = 5.33LVNNLDD65 pKa = 4.05PEE67 pKa = 4.18ITDD70 pKa = 3.17ILVIYY75 pKa = 10.23GGADD79 pKa = 3.0QTGWDD84 pKa = 3.75NGLSITNLDD93 pKa = 4.16LLKK96 pKa = 10.51KK97 pKa = 10.14DD98 pKa = 4.15LEE100 pKa = 4.65DD101 pKa = 5.18GIVGSDD107 pKa = 4.3EE108 pKa = 5.34DD109 pKa = 4.07PQTPTEE115 pKa = 4.07YY116 pKa = 11.09VLIRR120 pKa = 11.84ISDD123 pKa = 3.67VDD125 pKa = 3.35ISSPGALSEE134 pKa = 3.99GLQYY138 pKa = 11.24AEE140 pKa = 5.5SYY142 pKa = 10.81SADD145 pKa = 3.51NDD147 pKa = 3.75LASANRR153 pKa = 11.84YY154 pKa = 9.31LLFWNHH160 pKa = 5.2GGGYY164 pKa = 9.93EE165 pKa = 4.19GFGANEE171 pKa = 4.77LFDD174 pKa = 3.39TWMSVDD180 pKa = 4.27DD181 pKa = 4.66LQTGLAASKK190 pKa = 9.69PYY192 pKa = 10.5DD193 pKa = 3.57IIIFDD198 pKa = 4.41ACQMASLEE206 pKa = 4.31VADD209 pKa = 5.59ALDD212 pKa = 3.46THH214 pKa = 7.83ADD216 pKa = 3.41FLLASEE222 pKa = 4.77EE223 pKa = 4.07NVPGIGLNYY232 pKa = 10.1VGIGKK237 pKa = 9.67ALSSDD242 pKa = 3.21PAMTPEE248 pKa = 4.17EE249 pKa = 4.15VGKK252 pKa = 10.67SIISAYY258 pKa = 10.65VSGTNEE264 pKa = 3.55NRR266 pKa = 11.84KK267 pKa = 7.2TLSLVRR273 pKa = 11.84LQKK276 pKa = 10.7AKK278 pKa = 10.94NVVASLDD285 pKa = 3.62VLGTSLKK292 pKa = 10.87DD293 pKa = 3.31SLNNEE298 pKa = 4.28DD299 pKa = 4.58SLAALGDD306 pKa = 3.42IYY308 pKa = 9.55TTVQGYY314 pKa = 9.25GRR316 pKa = 11.84SDD318 pKa = 3.44DD319 pKa = 3.81GSRR322 pKa = 11.84VASVDD327 pKa = 3.71LYY329 pKa = 11.4QFADD333 pKa = 4.8LIYY336 pKa = 10.88ANTEE340 pKa = 3.85DD341 pKa = 3.62TTEE344 pKa = 3.92LHH346 pKa = 6.3RR347 pKa = 11.84AAGSLMTSLQDD358 pKa = 3.45YY359 pKa = 9.7VVAADD364 pKa = 3.84HH365 pKa = 6.08STHH368 pKa = 6.53FSAANGVSIAAPKK381 pKa = 10.62EE382 pKa = 4.14DD383 pKa = 3.64FTKK386 pKa = 10.78AIPQSIAFGRR396 pKa = 11.84NGWYY400 pKa = 9.9EE401 pKa = 3.93YY402 pKa = 10.45FSSYY406 pKa = 8.56MSKK409 pKa = 9.02TGTQSQPSASYY420 pKa = 10.9KK421 pKa = 10.66SGNTDD426 pKa = 2.18SRR428 pKa = 11.84TIVVHH433 pKa = 7.72DD434 pKa = 3.96DD435 pKa = 4.08TEE437 pKa = 4.72TTWAFADD444 pKa = 3.54YY445 pKa = 10.94VYY447 pKa = 10.13YY448 pKa = 10.42TDD450 pKa = 3.23SNYY453 pKa = 10.0IIVGQTPLEE462 pKa = 4.37EE463 pKa = 4.64IYY465 pKa = 10.96APSEE469 pKa = 4.19DD470 pKa = 4.18GLWISVPTGEE480 pKa = 4.81YY481 pKa = 10.56EE482 pKa = 4.01EE483 pKa = 5.2PDD485 pKa = 2.95WDD487 pKa = 3.99GSWFVLRR494 pKa = 11.84SDD496 pKa = 4.32GKK498 pKa = 9.86NADD501 pKa = 3.68VLVSMTYY508 pKa = 10.1AYY510 pKa = 10.57SVVEE514 pKa = 4.36DD515 pKa = 4.06EE516 pKa = 4.65SVKK519 pKa = 10.66DD520 pKa = 3.43VYY522 pKa = 10.95LIYY525 pKa = 11.05GNITRR530 pKa = 11.84EE531 pKa = 4.08INGKK535 pKa = 10.05DD536 pKa = 2.81ITHH539 pKa = 7.18PSVITAVVDD548 pKa = 3.55MNAMKK553 pKa = 10.19TNYY556 pKa = 10.06LFVEE560 pKa = 4.8SVSEE564 pKa = 4.23DD565 pKa = 2.66GWYY568 pKa = 10.69SRR570 pKa = 11.84TNLWGDD576 pKa = 3.65TKK578 pKa = 11.1LLPGDD583 pKa = 3.69VFTPLLEE590 pKa = 4.35VYY592 pKa = 10.47NEE594 pKa = 4.11EE595 pKa = 4.31EE596 pKa = 4.11EE597 pKa = 4.73SISTVYY603 pKa = 10.73SADD606 pKa = 3.22SFTFGDD612 pKa = 5.04DD613 pKa = 3.39PAEE616 pKa = 4.23DD617 pKa = 3.91LVFTALDD624 pKa = 3.63EE625 pKa = 4.64NNCYY629 pKa = 9.41WVVEE633 pKa = 4.06LDD635 pKa = 5.59DD636 pKa = 5.05YY637 pKa = 11.91LDD639 pKa = 5.17DD640 pKa = 4.41DD641 pKa = 4.25AFYY644 pKa = 11.16LEE646 pKa = 5.42EE647 pKa = 5.42PDD649 pKa = 5.44FPDD652 pKa = 3.08TGAAKK657 pKa = 10.5SPILPAGMFFGLAAAFLLIKK677 pKa = 10.44RR678 pKa = 11.84RR679 pKa = 3.68

Molecular weight: 74.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A2STJ4|A2STJ4_METLZ 5'-phosphoribosylglycinamide transformylase OS=Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z) OX=410358 GN=Mlab_1486 PE=3 SV=1
MM1 pKa = 7.66SKK3 pKa = 8.88LTKK6 pKa = 9.77SRR8 pKa = 11.84KK9 pKa = 8.87IRR11 pKa = 11.84LAKK14 pKa = 9.4ATQQNRR20 pKa = 11.84RR21 pKa = 11.84VPAWVMIKK29 pKa = 9.0TKK31 pKa = 9.53RR32 pKa = 11.84TVVSHH37 pKa = 5.98PKK39 pKa = 8.67RR40 pKa = 11.84RR41 pKa = 11.84NWRR44 pKa = 11.84RR45 pKa = 11.84STLKK49 pKa = 10.56VV50 pKa = 3.07

Molecular weight: 6.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1739

0

1739

522529

47

2772

300.5

33.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.525 ± 0.064

1.507 ± 0.027

5.526 ± 0.049

6.7 ± 0.055

3.93 ± 0.044

7.769 ± 0.052

1.835 ± 0.025

7.631 ± 0.057

5.504 ± 0.051

9.202 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.865 ± 0.029

3.562 ± 0.043

4.434 ± 0.032

2.512 ± 0.027

4.678 ± 0.051

6.03 ± 0.043

5.977 ± 0.065

7.476 ± 0.055

0.923 ± 0.021

3.416 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski