EBPR podovirus 3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8TUY0|F8TUY0_9CAUD Uncharacterized protein gp14 OS=EBPR podovirus 3 OX=1048517 GN=gp14 PE=4 SV=1
MM1 pKa = 7.63LFNKK5 pKa = 10.18FSEE8 pKa = 4.69RR9 pKa = 11.84IKK11 pKa = 10.61AASGGGGIPGGSTGQALVKK30 pKa = 10.78ASGTDD35 pKa = 3.41YY36 pKa = 11.19DD37 pKa = 4.58YY38 pKa = 11.83VWTSIPGTFTISEE51 pKa = 4.06GNYY54 pKa = 10.1VYY56 pKa = 10.37FAYY59 pKa = 10.23AADD62 pKa = 5.3DD63 pKa = 3.37IQINVDD69 pKa = 3.23EE70 pKa = 4.43MTATSGGVVPAPPNDD85 pKa = 2.96SSYY88 pKa = 11.34FLDD91 pKa = 4.57GTGTWSIPASGAGPSPTEE109 pKa = 3.69TRR111 pKa = 11.84IGFSAATNGATMIVNTTSAISPMTVHH137 pKa = 5.63TTDD140 pKa = 3.9PGTTNWDD147 pKa = 4.1DD148 pKa = 3.3VWIWAANTDD157 pKa = 3.89TVSRR161 pKa = 11.84TISLYY166 pKa = 10.57FGAHH170 pKa = 5.54SSIGDD175 pKa = 3.72QVVGSYY181 pKa = 10.86DD182 pKa = 4.09LPPGGAPVLICPGLFLQNSGVVEE205 pKa = 4.96AYY207 pKa = 10.46ASIANKK213 pKa = 10.0INLTGYY219 pKa = 9.61VNRR222 pKa = 11.84CTVV225 pKa = 2.68

Molecular weight: 23.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8TV16|F8TV16_9CAUD Uncharacterized protein gp54 OS=EBPR podovirus 3 OX=1048517 GN=gp54 PE=4 SV=1
MM1 pKa = 7.75KK2 pKa = 10.34INLRR6 pKa = 11.84KK7 pKa = 9.28IRR9 pKa = 11.84KK10 pKa = 8.62LLWEE14 pKa = 4.16LPIVKK19 pKa = 9.86EE20 pKa = 3.96YY21 pKa = 11.03LLSRR25 pKa = 11.84KK26 pKa = 8.01IQKK29 pKa = 9.68INRR32 pKa = 11.84KK33 pKa = 7.17EE34 pKa = 3.79NRR36 pKa = 11.84NRR38 pKa = 11.84NGG40 pKa = 2.93

Molecular weight: 5.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

11004

34

1844

203.8

22.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.197 ± 0.421

0.872 ± 0.155

5.707 ± 0.232

6.516 ± 0.326

4.28 ± 0.24

6.479 ± 0.289

1.936 ± 0.121

7.043 ± 0.294

6.57 ± 0.364

8.988 ± 0.281

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.808 ± 0.154

4.626 ± 0.166

4.553 ± 0.204

3.653 ± 0.248

4.671 ± 0.194

6.416 ± 0.372

6.534 ± 0.368

5.734 ± 0.183

1.054 ± 0.118

3.362 ± 0.216
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski