Amino acid dipepetide frequency for Sphingomonas spermidinifaciens

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	23.515AlaAla: 23.515 ± 0.238	1.049AlaCys: 1.049 ± 0.032	8.131AlaAsp: 8.131 ± 0.11	8.431AlaGlu: 8.431 ± 0.096	4.568AlaPhe: 4.568 ± 0.065	13.042AlaGly: 13.042 ± 0.144	2.342AlaHis: 2.342 ± 0.05	7.19AlaIle: 7.19 ± 0.109	3.744AlaLys: 3.744 ± 0.061	14.903AlaLeu: 14.903 ± 0.15	4.075AlaMet: 4.075 ± 0.074	3.023AlaAsn: 3.023 ± 0.055	7.377AlaPro: 7.377 ± 0.118	4.157AlaGln: 4.157 ± 0.073	11.53AlaArg: 11.53 ± 0.13	6.504AlaSer: 6.504 ± 0.088	7.113AlaThr: 7.113 ± 0.086	9.513AlaVal: 9.513 ± 0.113	1.799AlaTrp: 1.799 ± 0.048	2.518AlaTyr: 2.518 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	0.941CysAla: 0.941 ± 0.029	0.076CysCys: 0.076 ± 0.011	0.493CysAsp: 0.493 ± 0.018	0.368CysGlu: 0.368 ± 0.016	0.25CysPhe: 0.25 ± 0.018	0.789CysGly: 0.789 ± 0.03	0.188CysHis: 0.188 ± 0.017	0.283CysIle: 0.283 ± 0.017	0.113CysLys: 0.113 ± 0.012	0.634CysLeu: 0.634 ± 0.026	0.131CysMet: 0.131 ± 0.011	0.176CysAsn: 0.176 ± 0.013	0.428CysPro: 0.428 ± 0.022	0.166CysGln: 0.166 ± 0.014	0.538CysArg: 0.538 ± 0.025	0.382CysSer: 0.382 ± 0.019	0.351CysThr: 0.351 ± 0.018	0.498CysVal: 0.498 ± 0.023	0.092CysTrp: 0.092 ± 0.009	0.138CysTyr: 0.138 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	8.524AspAla: 8.524 ± 0.109	0.421AspCys: 0.421 ± 0.02	3.275AspAsp: 3.275 ± 0.073	3.302AspGlu: 3.302 ± 0.058	2.078AspPhe: 2.078 ± 0.043	5.498AspGly: 5.498 ± 0.099	1.252AspHis: 1.252 ± 0.037	2.369AspIle: 2.369 ± 0.048	1.414AspLys: 1.414 ± 0.035	5.845AspLeu: 5.845 ± 0.078	1.22AspMet: 1.22 ± 0.035	1.163AspAsn: 1.163 ± 0.036	4.069AspPro: 4.069 ± 0.063	1.647AspGln: 1.647 ± 0.04	5.515AspArg: 5.515 ± 0.086	1.978AspSer: 1.978 ± 0.046	2.639AspThr: 2.639 ± 0.058	4.068AspVal: 4.068 ± 0.06	1.191AspTrp: 1.191 ± 0.035	1.548AspTyr: 1.548 ± 0.039	0.0AspXaa: 0.0 ± 0.0
Glu	8.85GluAla: 8.85 ± 0.115	0.304GluCys: 0.304 ± 0.017	2.552GluAsp: 2.552 ± 0.047	2.601GluGlu: 2.601 ± 0.059	1.342GluPhe: 1.342 ± 0.034	5.021GluGly: 5.021 ± 0.074	1.107GluHis: 1.107 ± 0.034	2.546GluIle: 2.546 ± 0.052	1.601GluLys: 1.601 ± 0.042	4.992GluLeu: 4.992 ± 0.068	1.317GluMet: 1.317 ± 0.033	1.132GluAsn: 1.132 ± 0.033	2.776GluPro: 2.776 ± 0.053	1.873GluGln: 1.873 ± 0.043	5.694GluArg: 5.694 ± 0.088	1.986GluSer: 1.986 ± 0.043	2.952GluThr: 2.952 ± 0.056	4.049GluVal: 4.049 ± 0.065	0.816GluTrp: 0.816 ± 0.032	0.868GluTyr: 0.868 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	5.09PheAla: 5.09 ± 0.081	0.279PheCys: 0.279 ± 0.016	2.781PheAsp: 2.781 ± 0.054	2.042PheGlu: 2.042 ± 0.044	1.18PhePhe: 1.18 ± 0.039	3.562PheGly: 3.562 ± 0.068	0.67PheHis: 0.67 ± 0.024	1.179PheIle: 1.179 ± 0.036	0.75PheLys: 0.75 ± 0.029	2.984PheLeu: 2.984 ± 0.066	0.619PheMet: 0.619 ± 0.024	0.985PheAsn: 0.985 ± 0.039	1.422PhePro: 1.422 ± 0.039	0.833PheGln: 0.833 ± 0.028	2.193PheArg: 2.193 ± 0.048	1.711PheSer: 1.711 ± 0.042	1.92PheThr: 1.92 ± 0.046	2.828PheVal: 2.828 ± 0.06	0.542PheTrp: 0.542 ± 0.025	0.863PheTyr: 0.863 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	11.0GlyAla: 11.0 ± 0.129	0.787GlyCys: 0.787 ± 0.031	5.16GlyAsp: 5.16 ± 0.076	5.505GlyGlu: 5.505 ± 0.081	3.742GlyPhe: 3.742 ± 0.061	8.72GlyGly: 8.72 ± 0.124	1.778GlyHis: 1.778 ± 0.045	4.378GlyIle: 4.378 ± 0.068	2.678GlyLys: 2.678 ± 0.057	8.842GlyLeu: 8.842 ± 0.105	2.222GlyMet: 2.222 ± 0.044	2.155GlyAsn: 2.155 ± 0.064	3.821GlyPro: 3.821 ± 0.061	2.886GlyGln: 2.886 ± 0.055	7.466GlyArg: 7.466 ± 0.085	4.729GlySer: 4.729 ± 0.077	5.068GlyThr: 5.068 ± 0.096	6.732GlyVal: 6.732 ± 0.08	1.811GlyTrp: 1.811 ± 0.042	2.308GlyTyr: 2.308 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	2.523HisAla: 2.523 ± 0.059	0.195HisCys: 0.195 ± 0.014	1.157HisAsp: 1.157 ± 0.036	0.954HisGlu: 0.954 ± 0.032	0.727HisPhe: 0.727 ± 0.025	1.843HisGly: 1.843 ± 0.042	0.529HisHis: 0.529 ± 0.024	0.692HisIle: 0.692 ± 0.028	0.339HisLys: 0.339 ± 0.018	1.779HisLeu: 1.779 ± 0.041	0.363HisMet: 0.363 ± 0.02	0.374HisAsn: 0.374 ± 0.02	1.245HisPro: 1.245 ± 0.042	0.481HisGln: 0.481 ± 0.023	1.49HisArg: 1.49 ± 0.043	0.735HisSer: 0.735 ± 0.026	0.601HisThr: 0.601 ± 0.022	1.553HisVal: 1.553 ± 0.043	0.37HisTrp: 0.37 ± 0.018	0.48HisTyr: 0.48 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	7.889IleAla: 7.889 ± 0.092	0.332IleCys: 0.332 ± 0.021	3.988IleAsp: 3.988 ± 0.056	3.72IleGlu: 3.72 ± 0.06	1.302IlePhe: 1.302 ± 0.037	5.147IleGly: 5.147 ± 0.077	0.747IleHis: 0.747 ± 0.029	1.494IleIle: 1.494 ± 0.043	0.968IleLys: 0.968 ± 0.036	3.701IleLeu: 3.701 ± 0.068	0.624IleMet: 0.624 ± 0.027	1.098IleAsn: 1.098 ± 0.037	1.951IlePro: 1.951 ± 0.047	1.031IleGln: 1.031 ± 0.03	3.093IleArg: 3.093 ± 0.053	1.868IleSer: 1.868 ± 0.05	2.068IleThr: 2.068 ± 0.043	4.349IleVal: 4.349 ± 0.072	0.52IleTrp: 0.52 ± 0.023	0.881IleTyr: 0.881 ± 0.033	0.0IleXaa: 0.0 ± 0.0
Lys	3.628LysAla: 3.628 ± 0.064	0.117LysCys: 0.117 ± 0.01	1.258LysAsp: 1.258 ± 0.039	0.93LysGlu: 0.93 ± 0.035	0.658LysPhe: 0.658 ± 0.026	2.396LysGly: 2.396 ± 0.046	0.392LysHis: 0.392 ± 0.019	1.039LysIle: 1.039 ± 0.035	0.868LysLys: 0.868 ± 0.04	2.805LysLeu: 2.805 ± 0.06	0.557LysMet: 0.557 ± 0.024	0.539LysAsn: 0.539 ± 0.023	1.854LysPro: 1.854 ± 0.05	0.688LysGln: 0.688 ± 0.028	2.181LysArg: 2.181 ± 0.047	1.253LysSer: 1.253 ± 0.036	1.345LysThr: 1.345 ± 0.042	1.825LysVal: 1.825 ± 0.051	0.334LysTrp: 0.334 ± 0.016	0.471LysTyr: 0.471 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	15.378LeuAla: 15.378 ± 0.159	0.656LeuCys: 0.656 ± 0.027	6.277LeuAsp: 6.277 ± 0.085	4.44LeuGlu: 4.44 ± 0.063	3.528LeuPhe: 3.528 ± 0.068	8.623LeuGly: 8.623 ± 0.101	1.656LeuHis: 1.656 ± 0.039	4.805LeuIle: 4.805 ± 0.074	2.798LeuLys: 2.798 ± 0.061	9.051LeuLeu: 9.051 ± 0.149	2.075LeuMet: 2.075 ± 0.051	2.233LeuAsn: 2.233 ± 0.053	5.884LeuPro: 5.884 ± 0.073	2.274LeuGln: 2.274 ± 0.05	7.082LeuArg: 7.082 ± 0.098	5.721LeuSer: 5.721 ± 0.075	5.678LeuThr: 5.678 ± 0.08	7.47LeuVal: 7.47 ± 0.086	1.221LeuTrp: 1.221 ± 0.04	1.992LeuTyr: 1.992 ± 0.052	0.0LeuXaa: 0.0 ± 0.0
Met	3.151MetAla: 3.151 ± 0.058	0.128MetCys: 0.128 ± 0.013	0.887MetAsp: 0.887 ± 0.029	0.821MetGlu: 0.821 ± 0.031	0.631MetPhe: 0.631 ± 0.026	1.787MetGly: 1.787 ± 0.051	0.4MetHis: 0.4 ± 0.019	1.24MetIle: 1.24 ± 0.038	0.792MetLys: 0.792 ± 0.027	2.554MetLeu: 2.554 ± 0.054	0.562MetMet: 0.562 ± 0.026	0.562MetAsn: 0.562 ± 0.023	1.485MetPro: 1.485 ± 0.04	0.694MetGln: 0.694 ± 0.023	1.86MetArg: 1.86 ± 0.044	1.3MetSer: 1.3 ± 0.039	1.654MetThr: 1.654 ± 0.04	1.477MetVal: 1.477 ± 0.034	0.208MetTrp: 0.208 ± 0.013	0.261MetTyr: 0.261 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	3.092AsnAla: 3.092 ± 0.061	0.205AsnCys: 0.205 ± 0.015	1.278AsnAsp: 1.278 ± 0.039	1.018AsnGlu: 1.018 ± 0.033	0.795AsnPhe: 0.795 ± 0.028	2.146AsnGly: 2.146 ± 0.058	0.42AsnHis: 0.42 ± 0.023	1.055AsnIle: 1.055 ± 0.035	0.52AsnLys: 0.52 ± 0.024	2.396AsnLeu: 2.396 ± 0.054	0.423AsnMet: 0.423 ± 0.018	0.573AsnAsn: 0.573 ± 0.037	1.65AsnPro: 1.65 ± 0.04	0.724AsnGln: 0.724 ± 0.028	1.792AsnArg: 1.792 ± 0.041	0.97AsnSer: 0.97 ± 0.037	0.979AsnThr: 0.979 ± 0.036	1.711AsnVal: 1.711 ± 0.051	0.394AsnTrp: 0.394 ± 0.02	0.621AsnTyr: 0.621 ± 0.027	0.0AsnXaa: 0.0 ± 0.0
Pro	8.315ProAla: 8.315 ± 0.108	0.311ProCys: 0.311 ± 0.016	3.91ProAsp: 3.91 ± 0.075	3.503ProGlu: 3.503 ± 0.063	1.916ProPhe: 1.916 ± 0.047	5.309ProGly: 5.309 ± 0.074	0.945ProHis: 0.945 ± 0.032	2.546ProIle: 2.546 ± 0.047	1.313ProLys: 1.313 ± 0.04	5.054ProLeu: 5.054 ± 0.072	1.22ProMet: 1.22 ± 0.035	1.245ProAsn: 1.245 ± 0.038	3.349ProPro: 3.349 ± 0.075	1.608ProGln: 1.608 ± 0.043	3.662ProArg: 3.662 ± 0.061	2.585ProSer: 2.585 ± 0.054	2.773ProThr: 2.773 ± 0.048	4.582ProVal: 4.582 ± 0.068	0.688ProTrp: 0.688 ± 0.028	1.11ProTyr: 1.11 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	4.12GlnAla: 4.12 ± 0.07	0.193GlnCys: 0.193 ± 0.014	1.181GlnAsp: 1.181 ± 0.039	0.998GlnGlu: 0.998 ± 0.034	1.009GlnPhe: 1.009 ± 0.032	2.366GlnGly: 2.366 ± 0.043	0.527GlnHis: 0.527 ± 0.021	1.434GlnIle: 1.434 ± 0.045	0.72GlnLys: 0.72 ± 0.026	2.817GlnLeu: 2.817 ± 0.061	0.697GlnMet: 0.697 ± 0.029	0.7GlnAsn: 0.7 ± 0.026	1.82GlnPro: 1.82 ± 0.041	1.056GlnGln: 1.056 ± 0.037	2.523GlnArg: 2.523 ± 0.053	1.528GlnSer: 1.528 ± 0.037	1.571GlnThr: 1.571 ± 0.036	2.232GlnVal: 2.232 ± 0.047	0.388GlnTrp: 0.388 ± 0.017	0.544GlnTyr: 0.544 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	10.529ArgAla: 10.529 ± 0.12	0.497ArgCys: 0.497 ± 0.024	4.636ArgAsp: 4.636 ± 0.068	4.519ArgGlu: 4.519 ± 0.082	3.432ArgPhe: 3.432 ± 0.06	5.851ArgGly: 5.851 ± 0.08	1.724ArgHis: 1.724 ± 0.049	4.314ArgIle: 4.314 ± 0.066	1.666ArgLys: 1.666 ± 0.042	9.021ArgLeu: 9.021 ± 0.108	1.921ArgMet: 1.921 ± 0.038	1.655ArgAsn: 1.655 ± 0.043	4.36ArgPro: 4.36 ± 0.074	2.484ArgGln: 2.484 ± 0.047	6.909ArgArg: 6.909 ± 0.117	3.653ArgSer: 3.653 ± 0.07	3.917ArgThr: 3.917 ± 0.062	5.886ArgVal: 5.886 ± 0.09	1.331ArgTrp: 1.331 ± 0.037	1.905ArgTyr: 1.905 ± 0.046	0.0ArgXaa: 0.0 ± 0.0
Ser	6.146SerAla: 6.146 ± 0.071	0.339SerCys: 0.339 ± 0.02	2.728SerAsp: 2.728 ± 0.049	2.25SerGlu: 2.25 ± 0.051	1.961SerPhe: 1.961 ± 0.046	5.149SerGly: 5.149 ± 0.067	0.788SerHis: 0.788 ± 0.031	2.54SerIle: 2.54 ± 0.05	1.092SerLys: 1.092 ± 0.037	4.71SerLeu: 4.71 ± 0.071	1.056SerMet: 1.056 ± 0.034	1.205SerAsn: 1.205 ± 0.038	2.757SerPro: 2.757 ± 0.056	1.236SerGln: 1.236 ± 0.034	3.335SerArg: 3.335 ± 0.051	2.237SerSer: 2.237 ± 0.057	2.464SerThr: 2.464 ± 0.052	3.559SerVal: 3.559 ± 0.066	0.712SerTrp: 0.712 ± 0.025	1.146SerTyr: 1.146 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	6.744ThrAla: 6.744 ± 0.097	0.355ThrCys: 0.355 ± 0.018	2.865ThrAsp: 2.865 ± 0.055	2.318ThrGlu: 2.318 ± 0.048	1.724ThrPhe: 1.724 ± 0.041	5.467ThrGly: 5.467 ± 0.082	0.903ThrHis: 0.903 ± 0.029	2.874ThrIle: 2.874 ± 0.05	1.109ThrLys: 1.109 ± 0.034	6.049ThrLeu: 6.049 ± 0.073	1.116ThrMet: 1.116 ± 0.029	1.173ThrAsn: 1.173 ± 0.038	3.631ThrPro: 3.631 ± 0.064	1.361ThrGln: 1.361 ± 0.03	3.942ThrArg: 3.942 ± 0.061	2.38ThrSer: 2.38 ± 0.061	2.692ThrThr: 2.692 ± 0.055	4.039ThrVal: 4.039 ± 0.077	0.558ThrTrp: 0.558 ± 0.023	1.12ThrTyr: 1.12 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	10.966ValAla: 10.966 ± 0.123	0.467ValCys: 0.467 ± 0.019	4.28ValAsp: 4.28 ± 0.069	5.021ValGlu: 5.021 ± 0.075	2.214ValPhe: 2.214 ± 0.046	6.024ValGly: 6.024 ± 0.079	1.276ValHis: 1.276 ± 0.037	3.486ValIle: 3.486 ± 0.056	1.784ValLys: 1.784 ± 0.043	6.879ValLeu: 6.879 ± 0.084	1.586ValMet: 1.586 ± 0.046	1.812ValAsn: 1.812 ± 0.057	4.319ValPro: 4.319 ± 0.064	2.009ValGln: 2.009 ± 0.038	5.882ValArg: 5.882 ± 0.083	3.911ValSer: 3.911 ± 0.067	4.731ValThr: 4.731 ± 0.073	5.862ValVal: 5.862 ± 0.089	0.869ValTrp: 0.869 ± 0.028	1.273ValTyr: 1.273 ± 0.036	0.0ValXaa: 0.0 ± 0.0
Trp	1.467TrpAla: 1.467 ± 0.038	0.121TrpCys: 0.121 ± 0.01	0.707TrpAsp: 0.707 ± 0.025	0.589TrpGlu: 0.589 ± 0.024	0.585TrpPhe: 0.585 ± 0.026	0.998TrpGly: 0.998 ± 0.033	0.331TrpHis: 0.331 ± 0.019	0.629TrpIle: 0.629 ± 0.026	0.407TrpLys: 0.407 ± 0.02	1.778TrpLeu: 1.778 ± 0.051	0.32TrpMet: 0.32 ± 0.019	0.412TrpAsn: 0.412 ± 0.023	0.729TrpPro: 0.729 ± 0.029	0.569TrpGln: 0.569 ± 0.021	1.547TrpArg: 1.547 ± 0.036	0.872TrpSer: 0.872 ± 0.03	0.914TrpThr: 0.914 ± 0.031	0.875TrpVal: 0.875 ± 0.028	0.276TrpTrp: 0.276 ± 0.018	0.318TrpTyr: 0.318 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.657TyrAla: 2.657 ± 0.053	0.205TyrCys: 0.205 ± 0.015	1.473TyrAsp: 1.473 ± 0.044	1.049TyrGlu: 1.049 ± 0.033	0.787TyrPhe: 0.787 ± 0.024	2.052TyrGly: 2.052 ± 0.052	0.455TyrHis: 0.455 ± 0.021	0.677TyrIle: 0.677 ± 0.028	0.464TyrLys: 0.464 ± 0.024	2.045TyrLeu: 2.045 ± 0.047	0.346TyrMet: 0.346 ± 0.018	0.547TyrAsn: 0.547 ± 0.026	1.019TyrPro: 1.019 ± 0.032	0.649TyrGln: 0.649 ± 0.025	1.976TyrArg: 1.976 ± 0.046	1.104TyrSer: 1.104 ± 0.036	0.99TyrThr: 0.99 ± 0.033	1.544TyrVal: 1.544 ± 0.037	0.327TyrTrp: 0.327 ± 0.018	0.563TyrTyr: 0.563 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3376 proteins (1098682 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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