Amino acid dipepetide frequency for Escherichia phage J8-65

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.51AlaAla: 13.51 ± 1.492
0.943AlaCys: 0.943 ± 0.309
5.813AlaAsp: 5.813 ± 0.536
5.97AlaGlu: 5.97 ± 0.749
3.378AlaPhe: 3.378 ± 0.502
7.305AlaGly: 7.305 ± 0.957
1.571AlaHis: 1.571 ± 0.359
5.184AlaIle: 5.184 ± 0.696
4.87AlaLys: 4.87 ± 0.733
9.112AlaLeu: 9.112 ± 1.072
3.063AlaMet: 3.063 ± 0.682
4.949AlaAsn: 4.949 ± 0.616
3.927AlaPro: 3.927 ± 0.751
4.791AlaGln: 4.791 ± 1.029
5.106AlaArg: 5.106 ± 0.684
6.441AlaSer: 6.441 ± 0.872
6.598AlaThr: 6.598 ± 0.92
6.205AlaVal: 6.205 ± 0.756
1.335AlaTrp: 1.335 ± 0.291
3.22AlaTyr: 3.22 ± 0.515
0.0AlaXaa: 0.0 ± 0.0
Cys
0.943CysAla: 0.943 ± 0.401
0.0CysCys: 0.0 ± 0.0
0.393CysAsp: 0.393 ± 0.235
0.785CysGlu: 0.785 ± 0.345
0.236CysPhe: 0.236 ± 0.132
0.943CysGly: 0.943 ± 0.416
0.236CysHis: 0.236 ± 0.161
0.471CysIle: 0.471 ± 0.169
0.55CysLys: 0.55 ± 0.26
0.471CysLeu: 0.471 ± 0.174
0.393CysMet: 0.393 ± 0.184
0.314CysAsn: 0.314 ± 0.22
0.471CysPro: 0.471 ± 0.219
0.314CysGln: 0.314 ± 0.172
0.55CysArg: 0.55 ± 0.258
0.785CysSer: 0.785 ± 0.228
0.393CysThr: 0.393 ± 0.187
0.628CysVal: 0.628 ± 0.216
0.236CysTrp: 0.236 ± 0.119
0.55CysTyr: 0.55 ± 0.213
0.0CysXaa: 0.0 ± 0.0
Asp
6.048AspAla: 6.048 ± 0.581
0.393AspCys: 0.393 ± 0.18
4.791AspAsp: 4.791 ± 0.776
4.242AspGlu: 4.242 ± 0.667
2.121AspPhe: 2.121 ± 0.554
4.949AspGly: 4.949 ± 0.7
1.021AspHis: 1.021 ± 0.27
3.22AspIle: 3.22 ± 0.432
2.671AspLys: 2.671 ± 0.519
3.849AspLeu: 3.849 ± 0.475
2.356AspMet: 2.356 ± 0.566
2.671AspAsn: 2.671 ± 0.41
3.378AspPro: 3.378 ± 0.518
1.021AspGln: 1.021 ± 0.365
2.828AspArg: 2.828 ± 0.436
3.849AspSer: 3.849 ± 0.565
3.142AspThr: 3.142 ± 0.422
3.692AspVal: 3.692 ± 0.532
1.414AspTrp: 1.414 ± 0.338
2.278AspTyr: 2.278 ± 0.401
0.0AspXaa: 0.0 ± 0.0
Glu
5.813GluAla: 5.813 ± 0.893
0.55GluCys: 0.55 ± 0.228
2.985GluAsp: 2.985 ± 0.512
4.713GluGlu: 4.713 ± 0.881
2.828GluPhe: 2.828 ± 0.458
4.242GluGly: 4.242 ± 0.631
1.335GluHis: 1.335 ± 0.299
2.278GluIle: 2.278 ± 0.419
3.22GluLys: 3.22 ± 0.551
5.263GluLeu: 5.263 ± 0.834
1.964GluMet: 1.964 ± 0.396
1.964GluAsn: 1.964 ± 0.424
1.885GluPro: 1.885 ± 0.409
3.456GluGln: 3.456 ± 0.463
3.22GluArg: 3.22 ± 0.573
3.456GluSer: 3.456 ± 0.566
3.849GluThr: 3.849 ± 0.559
5.341GluVal: 5.341 ± 0.755
0.707GluTrp: 0.707 ± 0.261
3.142GluTyr: 3.142 ± 0.569
0.0GluXaa: 0.0 ± 0.0
Phe
2.356PheAla: 2.356 ± 0.473
0.55PheCys: 0.55 ± 0.181
3.142PheAsp: 3.142 ± 0.525
1.65PheGlu: 1.65 ± 0.339
0.785PhePhe: 0.785 ± 0.233
3.22PheGly: 3.22 ± 0.355
0.55PheHis: 0.55 ± 0.211
2.278PheIle: 2.278 ± 0.385
2.199PheLys: 2.199 ± 0.564
2.199PheLeu: 2.199 ± 0.389
1.178PheMet: 1.178 ± 0.255
2.121PheAsn: 2.121 ± 0.504
1.65PhePro: 1.65 ± 0.305
1.178PheGln: 1.178 ± 0.268
1.414PheArg: 1.414 ± 0.289
1.571PheSer: 1.571 ± 0.366
2.592PheThr: 2.592 ± 0.547
2.356PheVal: 2.356 ± 0.268
0.471PheTrp: 0.471 ± 0.167
0.785PheTyr: 0.785 ± 0.263
0.0PheXaa: 0.0 ± 0.0
Gly
7.305GlyAla: 7.305 ± 0.977
0.943GlyCys: 0.943 ± 0.287
4.32GlyAsp: 4.32 ± 0.631
4.242GlyGlu: 4.242 ± 0.618
1.964GlyPhe: 1.964 ± 0.412
6.362GlyGly: 6.362 ± 0.934
1.335GlyHis: 1.335 ± 0.401
4.949GlyIle: 4.949 ± 0.547
4.085GlyLys: 4.085 ± 0.828
5.97GlyLeu: 5.97 ± 0.573
1.964GlyMet: 1.964 ± 0.427
3.142GlyAsn: 3.142 ± 0.588
1.571GlyPro: 1.571 ± 0.33
4.006GlyGln: 4.006 ± 0.628
5.263GlyArg: 5.263 ± 0.602
4.556GlySer: 4.556 ± 0.881
5.184GlyThr: 5.184 ± 1.14
5.734GlyVal: 5.734 ± 1.045
1.414GlyTrp: 1.414 ± 0.3
3.063GlyTyr: 3.063 ± 0.433
0.0GlyXaa: 0.0 ± 0.0
His
2.199HisAla: 2.199 ± 0.474
0.314HisCys: 0.314 ± 0.143
1.65HisAsp: 1.65 ± 0.421
1.335HisGlu: 1.335 ± 0.388
0.628HisPhe: 0.628 ± 0.224
1.257HisGly: 1.257 ± 0.353
0.628HisHis: 0.628 ± 0.27
0.707HisIle: 0.707 ± 0.253
1.1HisLys: 1.1 ± 0.225
2.121HisLeu: 2.121 ± 0.458
0.55HisMet: 0.55 ± 0.176
0.55HisAsn: 0.55 ± 0.183
0.55HisPro: 0.55 ± 0.168
0.471HisGln: 0.471 ± 0.22
0.628HisArg: 0.628 ± 0.248
0.864HisSer: 0.864 ± 0.188
0.707HisThr: 0.707 ± 0.245
1.257HisVal: 1.257 ± 0.399
0.314HisTrp: 0.314 ± 0.202
0.707HisTyr: 0.707 ± 0.185
0.0HisXaa: 0.0 ± 0.0
Ile
4.006IleAla: 4.006 ± 0.578
0.628IleCys: 0.628 ± 0.361
3.456IleAsp: 3.456 ± 0.487
2.985IleGlu: 2.985 ± 0.416
1.414IlePhe: 1.414 ± 0.289
3.378IleGly: 3.378 ± 0.615
1.1IleHis: 1.1 ± 0.275
1.885IleIle: 1.885 ± 0.551
2.121IleLys: 2.121 ± 0.463
2.985IleLeu: 2.985 ± 0.432
1.414IleMet: 1.414 ± 0.245
2.278IleAsn: 2.278 ± 0.468
2.592IlePro: 2.592 ± 0.386
2.435IleGln: 2.435 ± 0.344
3.22IleArg: 3.22 ± 0.477
2.278IleSer: 2.278 ± 0.36
2.514IleThr: 2.514 ± 0.473
4.006IleVal: 4.006 ± 0.639
0.393IleTrp: 0.393 ± 0.197
1.964IleTyr: 1.964 ± 0.474
0.0IleXaa: 0.0 ± 0.0
Lys
5.577LysAla: 5.577 ± 0.921
0.628LysCys: 0.628 ± 0.196
2.514LysAsp: 2.514 ± 0.554
2.592LysGlu: 2.592 ± 0.382
1.885LysPhe: 1.885 ± 0.375
3.063LysGly: 3.063 ± 0.574
0.707LysHis: 0.707 ± 0.243
0.55LysIle: 0.55 ± 0.198
1.807LysLys: 1.807 ± 0.519
4.949LysLeu: 4.949 ± 0.946
1.021LysMet: 1.021 ± 0.255
1.257LysAsn: 1.257 ± 0.282
1.728LysPro: 1.728 ± 0.318
2.514LysGln: 2.514 ± 0.5
3.22LysArg: 3.22 ± 0.51
3.692LysSer: 3.692 ± 0.528
2.671LysThr: 2.671 ± 0.444
4.006LysVal: 4.006 ± 0.632
0.864LysTrp: 0.864 ± 0.268
1.335LysTyr: 1.335 ± 0.306
0.0LysXaa: 0.0 ± 0.0
Leu
9.112LeuAla: 9.112 ± 0.779
0.628LeuCys: 0.628 ± 0.263
5.341LeuAsp: 5.341 ± 0.739
5.42LeuGlu: 5.42 ± 0.486
2.985LeuPhe: 2.985 ± 0.568
5.341LeuGly: 5.341 ± 0.717
2.042LeuHis: 2.042 ± 0.459
3.299LeuIle: 3.299 ± 0.66
2.906LeuLys: 2.906 ± 0.462
6.598LeuLeu: 6.598 ± 0.836
1.807LeuMet: 1.807 ± 0.391
3.22LeuAsn: 3.22 ± 0.558
4.163LeuPro: 4.163 ± 0.693
4.085LeuGln: 4.085 ± 0.526
4.791LeuArg: 4.791 ± 0.721
5.498LeuSer: 5.498 ± 0.588
5.263LeuThr: 5.263 ± 0.645
4.399LeuVal: 4.399 ± 0.497
1.1LeuTrp: 1.1 ± 0.286
2.199LeuTyr: 2.199 ± 0.421
0.0LeuXaa: 0.0 ± 0.0
Met
2.671MetAla: 2.671 ± 0.392
0.236MetCys: 0.236 ± 0.132
1.335MetAsp: 1.335 ± 0.31
1.021MetGlu: 1.021 ± 0.276
0.785MetPhe: 0.785 ± 0.284
1.964MetGly: 1.964 ± 0.402
0.55MetHis: 0.55 ± 0.204
0.55MetIle: 0.55 ± 0.19
1.571MetLys: 1.571 ± 0.309
2.749MetLeu: 2.749 ± 0.549
1.414MetMet: 1.414 ± 0.469
1.65MetAsn: 1.65 ± 0.29
1.178MetPro: 1.178 ± 0.281
1.728MetGln: 1.728 ± 0.49
1.728MetArg: 1.728 ± 0.419
1.65MetSer: 1.65 ± 0.385
2.121MetThr: 2.121 ± 0.297
2.042MetVal: 2.042 ± 0.414
0.55MetTrp: 0.55 ± 0.231
1.178MetTyr: 1.178 ± 0.251
0.0MetXaa: 0.0 ± 0.0
Asn
4.163AsnAla: 4.163 ± 0.592
0.393AsnCys: 0.393 ± 0.199
1.728AsnAsp: 1.728 ± 0.294
2.356AsnGlu: 2.356 ± 0.445
1.807AsnPhe: 1.807 ± 0.364
3.77AsnGly: 3.77 ± 0.899
0.943AsnHis: 0.943 ± 0.35
3.299AsnIle: 3.299 ± 0.663
2.828AsnLys: 2.828 ± 0.424
2.199AsnLeu: 2.199 ± 0.396
1.571AsnMet: 1.571 ± 0.374
2.199AsnAsn: 2.199 ± 0.475
2.749AsnPro: 2.749 ± 0.456
2.514AsnGln: 2.514 ± 0.639
2.435AsnArg: 2.435 ± 0.404
2.514AsnSer: 2.514 ± 0.475
2.592AsnThr: 2.592 ± 0.406
2.828AsnVal: 2.828 ± 0.57
0.55AsnTrp: 0.55 ± 0.182
0.55AsnTyr: 0.55 ± 0.209
0.0AsnXaa: 0.0 ± 0.0
Pro
4.556ProAla: 4.556 ± 0.448
0.55ProCys: 0.55 ± 0.221
2.828ProAsp: 2.828 ± 0.398
4.006ProGlu: 4.006 ± 0.855
1.257ProPhe: 1.257 ± 0.233
3.142ProGly: 3.142 ± 0.46
0.785ProHis: 0.785 ± 0.241
1.964ProIle: 1.964 ± 0.368
1.335ProLys: 1.335 ± 0.403
2.514ProLeu: 2.514 ± 0.481
0.628ProMet: 0.628 ± 0.22
2.278ProAsn: 2.278 ± 0.468
1.492ProPro: 1.492 ± 0.438
2.514ProGln: 2.514 ± 1.086
1.807ProArg: 1.807 ± 0.319
2.278ProSer: 2.278 ± 0.469
3.063ProThr: 3.063 ± 0.505
2.906ProVal: 2.906 ± 0.503
0.628ProTrp: 0.628 ± 0.24
1.964ProTyr: 1.964 ± 0.522
0.0ProXaa: 0.0 ± 0.0
Gln
5.734GlnAla: 5.734 ± 1.359
0.314GlnCys: 0.314 ± 0.134
2.199GlnAsp: 2.199 ± 0.511
2.514GlnGlu: 2.514 ± 0.505
2.514GlnPhe: 2.514 ± 0.47
3.456GlnGly: 3.456 ± 0.656
0.864GlnHis: 0.864 ± 0.218
1.885GlnIle: 1.885 ± 0.463
1.807GlnLys: 1.807 ± 0.399
4.242GlnLeu: 4.242 ± 0.611
1.492GlnMet: 1.492 ± 0.294
1.65GlnAsn: 1.65 ± 0.496
2.121GlnPro: 2.121 ± 1.03
4.242GlnGln: 4.242 ± 1.273
3.692GlnArg: 3.692 ± 0.66
2.121GlnSer: 2.121 ± 0.513
2.828GlnThr: 2.828 ± 0.633
3.378GlnVal: 3.378 ± 0.642
0.864GlnTrp: 0.864 ± 0.21
2.042GlnTyr: 2.042 ± 0.429
0.0GlnXaa: 0.0 ± 0.0
Arg
5.577ArgAla: 5.577 ± 0.987
0.628ArgCys: 0.628 ± 0.205
3.142ArgAsp: 3.142 ± 0.46
4.006ArgGlu: 4.006 ± 0.628
1.885ArgPhe: 1.885 ± 0.382
4.399ArgGly: 4.399 ± 0.417
0.864ArgHis: 0.864 ± 0.252
3.142ArgIle: 3.142 ± 0.537
2.906ArgLys: 2.906 ± 0.443
4.556ArgLeu: 4.556 ± 0.547
1.728ArgMet: 1.728 ± 0.389
2.121ArgAsn: 2.121 ± 0.441
1.728ArgPro: 1.728 ± 0.479
2.906ArgGln: 2.906 ± 0.507
4.006ArgArg: 4.006 ± 0.788
2.121ArgSer: 2.121 ± 0.406
3.22ArgThr: 3.22 ± 0.575
3.849ArgVal: 3.849 ± 0.53
0.707ArgTrp: 0.707 ± 0.231
2.356ArgTyr: 2.356 ± 0.438
0.0ArgXaa: 0.0 ± 0.0
Ser
6.127SerAla: 6.127 ± 0.588
0.0SerCys: 0.0 ± 0.0
3.063SerAsp: 3.063 ± 0.476
2.828SerGlu: 2.828 ± 0.463
1.807SerPhe: 1.807 ± 0.391
6.205SerGly: 6.205 ± 0.617
0.55SerHis: 0.55 ± 0.22
2.828SerIle: 2.828 ± 0.413
2.985SerLys: 2.985 ± 0.595
4.634SerLeu: 4.634 ± 0.473
1.728SerMet: 1.728 ± 0.388
3.456SerAsn: 3.456 ± 0.694
3.22SerPro: 3.22 ± 0.608
2.671SerGln: 2.671 ± 0.66
2.199SerArg: 2.199 ± 0.426
2.278SerSer: 2.278 ± 0.656
3.142SerThr: 3.142 ± 0.745
3.927SerVal: 3.927 ± 0.738
0.785SerTrp: 0.785 ± 0.244
2.435SerTyr: 2.435 ± 0.504
0.0SerXaa: 0.0 ± 0.0
Thr
7.226ThrAla: 7.226 ± 0.847
0.471ThrCys: 0.471 ± 0.17
3.22ThrAsp: 3.22 ± 0.5
2.828ThrGlu: 2.828 ± 0.409
2.514ThrPhe: 2.514 ± 0.465
5.577ThrGly: 5.577 ± 0.658
0.628ThrHis: 0.628 ± 0.211
2.435ThrIle: 2.435 ± 0.418
3.063ThrLys: 3.063 ± 0.558
5.498ThrLeu: 5.498 ± 0.716
1.257ThrMet: 1.257 ± 0.299
2.514ThrAsn: 2.514 ± 0.461
2.435ThrPro: 2.435 ± 0.523
3.063ThrGln: 3.063 ± 0.622
2.749ThrArg: 2.749 ± 0.302
3.927ThrSer: 3.927 ± 0.84
2.985ThrThr: 2.985 ± 0.983
5.97ThrVal: 5.97 ± 1.047
0.471ThrTrp: 0.471 ± 0.164
2.671ThrTyr: 2.671 ± 0.405
0.0ThrXaa: 0.0 ± 0.0
Val
6.441ValAla: 6.441 ± 0.888
0.628ValCys: 0.628 ± 0.214
4.085ValAsp: 4.085 ± 0.514
5.42ValGlu: 5.42 ± 0.683
1.885ValPhe: 1.885 ± 0.498
5.106ValGly: 5.106 ± 0.598
1.571ValHis: 1.571 ± 0.351
3.927ValIle: 3.927 ± 0.737
3.299ValLys: 3.299 ± 0.561
5.42ValLeu: 5.42 ± 0.736
1.414ValMet: 1.414 ± 0.314
3.613ValAsn: 3.613 ± 0.625
3.456ValPro: 3.456 ± 0.401
3.613ValGln: 3.613 ± 0.584
4.006ValArg: 4.006 ± 0.754
3.692ValSer: 3.692 ± 0.567
4.949ValThr: 4.949 ± 0.751
4.634ValVal: 4.634 ± 0.686
0.943ValTrp: 0.943 ± 0.244
2.514ValTyr: 2.514 ± 0.435
0.0ValXaa: 0.0 ± 0.0
Trp
0.943TrpAla: 0.943 ± 0.251
0.628TrpCys: 0.628 ± 0.232
1.414TrpAsp: 1.414 ± 0.272
0.785TrpGlu: 0.785 ± 0.28
0.707TrpPhe: 0.707 ± 0.232
0.864TrpGly: 0.864 ± 0.279
0.236TrpHis: 0.236 ± 0.121
0.55TrpIle: 0.55 ± 0.183
0.236TrpLys: 0.236 ± 0.139
1.807TrpLeu: 1.807 ± 0.344
0.314TrpMet: 0.314 ± 0.158
0.471TrpAsn: 0.471 ± 0.148
0.55TrpPro: 0.55 ± 0.219
0.628TrpGln: 0.628 ± 0.189
0.628TrpArg: 0.628 ± 0.219
1.1TrpSer: 1.1 ± 0.281
0.707TrpThr: 0.707 ± 0.189
0.707TrpVal: 0.707 ± 0.214
0.393TrpTrp: 0.393 ± 0.22
0.55TrpTyr: 0.55 ± 0.237
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.985TyrAla: 2.985 ± 0.536
0.236TyrCys: 0.236 ± 0.159
2.514TyrAsp: 2.514 ± 0.475
2.514TyrGlu: 2.514 ± 0.469
0.864TyrPhe: 0.864 ± 0.252
2.828TyrGly: 2.828 ± 0.407
1.021TyrHis: 1.021 ± 0.309
1.65TyrIle: 1.65 ± 0.448
1.021TyrLys: 1.021 ± 0.283
3.142TyrLeu: 3.142 ± 0.628
1.335TyrMet: 1.335 ± 0.251
1.571TyrAsn: 1.571 ± 0.285
1.65TyrPro: 1.65 ± 0.333
1.807TyrGln: 1.807 ± 0.345
2.356TyrArg: 2.356 ± 0.578
2.199TyrSer: 2.199 ± 0.389
2.985TyrThr: 2.985 ± 0.598
2.749TyrVal: 2.749 ± 0.583
0.079TyrTrp: 0.079 ± 0.071
1.257TyrTyr: 1.257 ± 0.308
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 47 proteins (12732 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski