Amino acid dipepetide frequency for Paenibacillus phage PG1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.04AlaAla: 6.04 ± 0.703
0.661AlaCys: 0.661 ± 0.224
4.436AlaAsp: 4.436 ± 0.566
4.624AlaGlu: 4.624 ± 0.664
3.209AlaPhe: 3.209 ± 0.527
4.058AlaGly: 4.058 ± 0.5
0.849AlaHis: 0.849 ± 0.316
4.436AlaIle: 4.436 ± 0.676
5.568AlaLys: 5.568 ± 0.732
6.606AlaLeu: 6.606 ± 0.976
1.793AlaMet: 1.793 ± 0.365
4.247AlaAsn: 4.247 ± 0.641
1.982AlaPro: 1.982 ± 0.298
3.114AlaGln: 3.114 ± 0.635
2.171AlaArg: 2.171 ± 0.338
3.586AlaSer: 3.586 ± 0.815
3.114AlaThr: 3.114 ± 0.666
5.379AlaVal: 5.379 ± 0.708
1.321AlaTrp: 1.321 ± 0.34
2.643AlaTyr: 2.643 ± 0.437
0.0AlaXaa: 0.0 ± 0.0
Cys
0.566CysAla: 0.566 ± 0.214
0.094CysCys: 0.094 ± 0.093
0.566CysAsp: 0.566 ± 0.244
1.227CysGlu: 1.227 ± 0.528
0.094CysPhe: 0.094 ± 0.098
1.133CysGly: 1.133 ± 0.446
0.661CysHis: 0.661 ± 0.26
0.283CysIle: 0.283 ± 0.186
0.472CysLys: 0.472 ± 0.197
0.378CysLeu: 0.378 ± 0.174
0.189CysMet: 0.189 ± 0.107
0.189CysAsn: 0.189 ± 0.12
0.378CysPro: 0.378 ± 0.185
0.283CysGln: 0.283 ± 0.159
0.472CysArg: 0.472 ± 0.224
0.566CysSer: 0.566 ± 0.343
0.566CysThr: 0.566 ± 0.248
0.849CysVal: 0.849 ± 0.253
0.189CysTrp: 0.189 ± 0.148
0.189CysTyr: 0.189 ± 0.19
0.0CysXaa: 0.0 ± 0.0
Asp
5.002AspAla: 5.002 ± 0.608
0.566AspCys: 0.566 ± 0.235
2.737AspAsp: 2.737 ± 0.54
5.285AspGlu: 5.285 ± 0.832
2.359AspPhe: 2.359 ± 0.507
5.851AspGly: 5.851 ± 0.777
0.944AspHis: 0.944 ± 0.288
4.153AspIle: 4.153 ± 0.592
4.624AspLys: 4.624 ± 0.676
5.568AspLeu: 5.568 ± 0.69
1.416AspMet: 1.416 ± 0.395
3.114AspAsn: 3.114 ± 0.546
2.926AspPro: 2.926 ± 0.526
1.888AspGln: 1.888 ± 0.361
2.359AspArg: 2.359 ± 0.462
3.303AspSer: 3.303 ± 0.682
2.359AspThr: 2.359 ± 0.379
3.114AspVal: 3.114 ± 0.528
0.849AspTrp: 0.849 ± 0.307
3.681AspTyr: 3.681 ± 0.626
0.0AspXaa: 0.0 ± 0.0
Glu
5.568GluAla: 5.568 ± 0.689
1.038GluCys: 1.038 ± 0.36
4.058GluAsp: 4.058 ± 0.549
6.795GluGlu: 6.795 ± 1.17
3.209GluPhe: 3.209 ± 0.615
5.002GluGly: 5.002 ± 0.673
1.982GluHis: 1.982 ± 0.374
6.701GluIle: 6.701 ± 0.701
4.908GluLys: 4.908 ± 0.664
7.267GluLeu: 7.267 ± 0.882
3.114GluMet: 3.114 ± 0.53
3.492GluAsn: 3.492 ± 0.534
1.888GluPro: 1.888 ± 0.495
3.869GluGln: 3.869 ± 0.589
4.813GluArg: 4.813 ± 0.696
4.436GluSer: 4.436 ± 0.63
3.869GluThr: 3.869 ± 0.504
6.134GluVal: 6.134 ± 0.91
1.982GluTrp: 1.982 ± 0.613
3.02GluTyr: 3.02 ± 0.538
0.0GluXaa: 0.0 ± 0.0
Phe
2.737PheAla: 2.737 ± 0.34
0.283PheCys: 0.283 ± 0.227
3.303PheAsp: 3.303 ± 0.57
3.398PheGlu: 3.398 ± 0.719
1.133PhePhe: 1.133 ± 0.386
2.076PheGly: 2.076 ± 0.414
0.378PheHis: 0.378 ± 0.167
2.454PheIle: 2.454 ± 0.348
2.831PheLys: 2.831 ± 0.502
2.926PheLeu: 2.926 ± 0.465
0.661PheMet: 0.661 ± 0.22
2.926PheAsn: 2.926 ± 0.673
1.227PhePro: 1.227 ± 0.387
1.227PheGln: 1.227 ± 0.314
0.944PheArg: 0.944 ± 0.261
2.548PheSer: 2.548 ± 0.467
2.076PheThr: 2.076 ± 0.434
2.076PheVal: 2.076 ± 0.385
0.378PheTrp: 0.378 ± 0.196
1.604PheTyr: 1.604 ± 0.434
0.0PheXaa: 0.0 ± 0.0
Gly
3.964GlyAla: 3.964 ± 0.716
0.566GlyCys: 0.566 ± 0.224
4.624GlyAsp: 4.624 ± 0.585
5.191GlyGlu: 5.191 ± 0.599
3.775GlyPhe: 3.775 ± 0.656
4.341GlyGly: 4.341 ± 0.732
1.416GlyHis: 1.416 ± 0.331
5.851GlyIle: 5.851 ± 1.081
5.568GlyLys: 5.568 ± 0.777
5.568GlyLeu: 5.568 ± 0.676
2.737GlyMet: 2.737 ± 0.456
3.209GlyAsn: 3.209 ± 0.629
1.416GlyPro: 1.416 ± 0.297
2.171GlyGln: 2.171 ± 0.396
3.02GlyArg: 3.02 ± 0.496
4.058GlySer: 4.058 ± 0.641
3.964GlyThr: 3.964 ± 0.912
3.398GlyVal: 3.398 ± 0.538
0.849GlyTrp: 0.849 ± 0.245
3.681GlyTyr: 3.681 ± 0.533
0.0GlyXaa: 0.0 ± 0.0
His
0.849HisAla: 0.849 ± 0.248
0.094HisCys: 0.094 ± 0.088
0.944HisAsp: 0.944 ± 0.302
2.076HisGlu: 2.076 ± 0.484
0.661HisPhe: 0.661 ± 0.273
0.661HisGly: 0.661 ± 0.254
0.378HisHis: 0.378 ± 0.247
1.416HisIle: 1.416 ± 0.349
0.944HisLys: 0.944 ± 0.247
1.793HisLeu: 1.793 ± 0.39
0.566HisMet: 0.566 ± 0.217
0.755HisAsn: 0.755 ± 0.245
0.378HisPro: 0.378 ± 0.218
1.133HisGln: 1.133 ± 0.35
0.566HisArg: 0.566 ± 0.214
0.661HisSer: 0.661 ± 0.273
0.661HisThr: 0.661 ± 0.273
1.038HisVal: 1.038 ± 0.322
0.283HisTrp: 0.283 ± 0.143
1.038HisTyr: 1.038 ± 0.284
0.0HisXaa: 0.0 ± 0.0
Ile
3.303IleAla: 3.303 ± 0.498
0.944IleCys: 0.944 ± 0.224
5.379IleAsp: 5.379 ± 0.679
6.418IleGlu: 6.418 ± 0.893
1.982IlePhe: 1.982 ± 0.605
4.53IleGly: 4.53 ± 0.724
1.982IleHis: 1.982 ± 0.425
5.191IleIle: 5.191 ± 0.728
6.04IleLys: 6.04 ± 0.941
4.436IleLeu: 4.436 ± 0.653
1.133IleMet: 1.133 ± 0.335
4.341IleAsn: 4.341 ± 0.667
2.265IlePro: 2.265 ± 0.522
2.643IleGln: 2.643 ± 0.345
3.681IleArg: 3.681 ± 0.591
3.586IleSer: 3.586 ± 0.56
4.436IleThr: 4.436 ± 0.681
4.719IleVal: 4.719 ± 0.612
0.944IleTrp: 0.944 ± 0.333
2.076IleTyr: 2.076 ± 0.381
0.0IleXaa: 0.0 ± 0.0
Lys
6.134LysAla: 6.134 ± 0.643
0.472LysCys: 0.472 ± 0.19
3.964LysAsp: 3.964 ± 0.541
7.267LysGlu: 7.267 ± 0.851
3.114LysPhe: 3.114 ± 0.451
4.719LysGly: 4.719 ± 0.806
1.604LysHis: 1.604 ± 0.338
4.813LysIle: 4.813 ± 0.618
6.418LysLys: 6.418 ± 0.843
4.908LysLeu: 4.908 ± 0.804
2.076LysMet: 2.076 ± 0.384
2.643LysAsn: 2.643 ± 0.496
2.454LysPro: 2.454 ± 0.499
2.926LysGln: 2.926 ± 0.508
2.926LysArg: 2.926 ± 0.555
4.436LysSer: 4.436 ± 0.701
4.341LysThr: 4.341 ± 0.59
5.191LysVal: 5.191 ± 0.766
1.038LysTrp: 1.038 ± 0.284
3.02LysTyr: 3.02 ± 0.535
0.0LysXaa: 0.0 ± 0.0
Leu
5.096LeuAla: 5.096 ± 0.686
0.661LeuCys: 0.661 ± 0.247
5.379LeuAsp: 5.379 ± 0.733
6.323LeuGlu: 6.323 ± 0.675
2.643LeuPhe: 2.643 ± 0.468
6.229LeuGly: 6.229 ± 0.742
1.227LeuHis: 1.227 ± 0.323
5.285LeuIle: 5.285 ± 0.627
5.757LeuLys: 5.757 ± 0.972
6.418LeuLeu: 6.418 ± 0.927
2.076LeuMet: 2.076 ± 0.329
4.436LeuAsn: 4.436 ± 0.433
2.643LeuPro: 2.643 ± 0.395
3.114LeuGln: 3.114 ± 0.523
3.586LeuArg: 3.586 ± 0.839
5.191LeuSer: 5.191 ± 0.634
3.681LeuThr: 3.681 ± 0.548
4.719LeuVal: 4.719 ± 0.621
1.038LeuTrp: 1.038 ± 0.416
2.076LeuTyr: 2.076 ± 0.415
0.0LeuXaa: 0.0 ± 0.0
Met
2.265MetAla: 2.265 ± 0.552
0.0MetCys: 0.0 ± 0.0
2.171MetAsp: 2.171 ± 0.36
2.076MetGlu: 2.076 ± 0.494
0.566MetPhe: 0.566 ± 0.22
1.982MetGly: 1.982 ± 0.372
0.094MetHis: 0.094 ± 0.089
1.888MetIle: 1.888 ± 0.362
3.398MetLys: 3.398 ± 0.585
1.793MetLeu: 1.793 ± 0.402
0.661MetMet: 0.661 ± 0.313
2.076MetAsn: 2.076 ± 0.403
0.283MetPro: 0.283 ± 0.154
1.793MetGln: 1.793 ± 0.429
1.038MetArg: 1.038 ± 0.402
1.416MetSer: 1.416 ± 0.294
1.133MetThr: 1.133 ± 0.326
2.076MetVal: 2.076 ± 0.395
0.378MetTrp: 0.378 ± 0.165
0.944MetTyr: 0.944 ± 0.283
0.0MetXaa: 0.0 ± 0.0
Asn
4.436AsnAla: 4.436 ± 0.615
0.661AsnCys: 0.661 ± 0.335
2.265AsnAsp: 2.265 ± 0.514
4.436AsnGlu: 4.436 ± 0.595
1.321AsnPhe: 1.321 ± 0.417
4.53AsnGly: 4.53 ± 0.514
1.227AsnHis: 1.227 ± 0.283
3.303AsnIle: 3.303 ± 0.614
4.719AsnLys: 4.719 ± 0.587
3.492AsnLeu: 3.492 ± 0.579
0.944AsnMet: 0.944 ± 0.272
3.775AsnAsn: 3.775 ± 0.672
2.643AsnPro: 2.643 ± 0.622
1.227AsnGln: 1.227 ± 0.291
3.586AsnArg: 3.586 ± 0.582
2.737AsnSer: 2.737 ± 0.473
2.548AsnThr: 2.548 ± 0.556
3.114AsnVal: 3.114 ± 0.604
0.755AsnTrp: 0.755 ± 0.252
1.227AsnTyr: 1.227 ± 0.279
0.0AsnXaa: 0.0 ± 0.0
Pro
1.416ProAla: 1.416 ± 0.341
0.0ProCys: 0.0 ± 0.0
2.548ProAsp: 2.548 ± 0.48
2.076ProGlu: 2.076 ± 0.391
1.038ProPhe: 1.038 ± 0.33
1.982ProGly: 1.982 ± 0.512
0.378ProHis: 0.378 ± 0.215
1.888ProIle: 1.888 ± 0.345
3.209ProLys: 3.209 ± 0.531
2.643ProLeu: 2.643 ± 0.529
0.755ProMet: 0.755 ± 0.278
1.793ProAsn: 1.793 ± 0.358
0.849ProPro: 0.849 ± 0.243
0.944ProGln: 0.944 ± 0.283
1.321ProArg: 1.321 ± 0.369
2.548ProSer: 2.548 ± 0.574
1.604ProThr: 1.604 ± 0.333
3.114ProVal: 3.114 ± 0.48
0.755ProTrp: 0.755 ± 0.29
1.604ProTyr: 1.604 ± 0.425
0.0ProXaa: 0.0 ± 0.0
Gln
3.209GlnAla: 3.209 ± 0.619
0.189GlnCys: 0.189 ± 0.136
1.793GlnAsp: 1.793 ± 0.342
2.643GlnGlu: 2.643 ± 0.548
1.227GlnPhe: 1.227 ± 0.307
2.359GlnGly: 2.359 ± 0.498
0.661GlnHis: 0.661 ± 0.258
2.171GlnIle: 2.171 ± 0.533
2.643GlnLys: 2.643 ± 0.474
3.209GlnLeu: 3.209 ± 0.677
1.604GlnMet: 1.604 ± 0.39
1.888GlnAsn: 1.888 ± 0.385
1.133GlnPro: 1.133 ± 0.267
2.265GlnGln: 2.265 ± 0.499
2.454GlnArg: 2.454 ± 0.507
2.454GlnSer: 2.454 ± 0.401
2.926GlnThr: 2.926 ± 0.424
2.265GlnVal: 2.265 ± 0.465
0.472GlnTrp: 0.472 ± 0.219
1.51GlnTyr: 1.51 ± 0.306
0.0GlnXaa: 0.0 ± 0.0
Arg
3.114ArgAla: 3.114 ± 0.448
1.227ArgCys: 1.227 ± 0.511
2.171ArgAsp: 2.171 ± 0.434
4.247ArgGlu: 4.247 ± 0.823
1.416ArgPhe: 1.416 ± 0.416
3.114ArgGly: 3.114 ± 0.52
0.566ArgHis: 0.566 ± 0.238
3.209ArgIle: 3.209 ± 0.541
3.02ArgLys: 3.02 ± 0.514
3.492ArgLeu: 3.492 ± 0.708
1.604ArgMet: 1.604 ± 0.402
2.454ArgAsn: 2.454 ± 0.476
1.416ArgPro: 1.416 ± 0.424
1.982ArgGln: 1.982 ± 0.428
2.831ArgArg: 2.831 ± 0.547
1.888ArgSer: 1.888 ± 0.391
2.831ArgThr: 2.831 ± 0.432
3.209ArgVal: 3.209 ± 0.578
1.038ArgTrp: 1.038 ± 0.266
1.793ArgTyr: 1.793 ± 0.458
0.0ArgXaa: 0.0 ± 0.0
Ser
4.247SerAla: 4.247 ± 0.656
0.189SerCys: 0.189 ± 0.152
4.058SerAsp: 4.058 ± 0.642
4.624SerGlu: 4.624 ± 0.623
2.359SerPhe: 2.359 ± 0.411
5.285SerGly: 5.285 ± 0.649
0.944SerHis: 0.944 ± 0.295
3.964SerIle: 3.964 ± 0.546
3.586SerLys: 3.586 ± 0.495
3.398SerLeu: 3.398 ± 0.536
1.699SerMet: 1.699 ± 0.389
2.171SerAsn: 2.171 ± 0.448
1.51SerPro: 1.51 ± 0.363
1.982SerGln: 1.982 ± 0.422
3.114SerArg: 3.114 ± 0.475
3.114SerSer: 3.114 ± 0.579
2.454SerThr: 2.454 ± 0.483
3.964SerVal: 3.964 ± 0.495
1.133SerTrp: 1.133 ± 0.29
2.643SerTyr: 2.643 ± 0.49
0.0SerXaa: 0.0 ± 0.0
Thr
3.681ThrAla: 3.681 ± 0.497
0.661ThrCys: 0.661 ± 0.31
3.681ThrAsp: 3.681 ± 0.634
4.058ThrGlu: 4.058 ± 0.595
1.888ThrPhe: 1.888 ± 0.478
3.964ThrGly: 3.964 ± 0.66
0.472ThrHis: 0.472 ± 0.183
4.341ThrIle: 4.341 ± 0.513
3.303ThrLys: 3.303 ± 0.712
4.719ThrLeu: 4.719 ± 0.599
1.321ThrMet: 1.321 ± 0.365
2.548ThrAsn: 2.548 ± 0.564
2.076ThrPro: 2.076 ± 0.441
1.416ThrGln: 1.416 ± 0.372
2.454ThrArg: 2.454 ± 0.467
3.586ThrSer: 3.586 ± 0.372
3.303ThrThr: 3.303 ± 0.719
3.869ThrVal: 3.869 ± 0.431
0.755ThrTrp: 0.755 ± 0.262
1.982ThrTyr: 1.982 ± 0.453
0.0ThrXaa: 0.0 ± 0.0
Val
4.719ValAla: 4.719 ± 0.597
0.661ValCys: 0.661 ± 0.275
4.53ValAsp: 4.53 ± 0.634
5.474ValGlu: 5.474 ± 0.706
2.926ValPhe: 2.926 ± 0.373
3.586ValGly: 3.586 ± 0.596
0.755ValHis: 0.755 ± 0.304
4.247ValIle: 4.247 ± 0.613
4.908ValLys: 4.908 ± 0.645
4.436ValLeu: 4.436 ± 0.563
1.604ValMet: 1.604 ± 0.382
4.247ValAsn: 4.247 ± 0.509
2.926ValPro: 2.926 ± 0.508
2.831ValGln: 2.831 ± 0.496
2.643ValArg: 2.643 ± 0.567
3.681ValSer: 3.681 ± 0.524
4.247ValThr: 4.247 ± 0.591
4.53ValVal: 4.53 ± 0.675
0.378ValTrp: 0.378 ± 0.194
2.359ValTyr: 2.359 ± 0.501
0.0ValXaa: 0.0 ± 0.0
Trp
0.661TrpAla: 0.661 ± 0.225
0.0TrpCys: 0.0 ± 0.0
1.038TrpAsp: 1.038 ± 0.356
1.699TrpGlu: 1.699 ± 0.339
0.661TrpPhe: 0.661 ± 0.253
0.944TrpGly: 0.944 ± 0.352
0.094TrpHis: 0.094 ± 0.089
1.227TrpIle: 1.227 ± 0.366
1.038TrpLys: 1.038 ± 0.297
1.793TrpLeu: 1.793 ± 0.372
0.378TrpMet: 0.378 ± 0.196
1.133TrpAsn: 1.133 ± 0.328
0.094TrpPro: 0.094 ± 0.086
0.472TrpGln: 0.472 ± 0.183
0.755TrpArg: 0.755 ± 0.244
0.661TrpSer: 0.661 ± 0.221
1.227TrpThr: 1.227 ± 0.356
0.472TrpVal: 0.472 ± 0.221
0.472TrpTrp: 0.472 ± 0.226
0.472TrpTyr: 0.472 ± 0.245
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.831TyrAla: 2.831 ± 0.503
0.378TyrCys: 0.378 ± 0.19
2.454TyrAsp: 2.454 ± 0.525
3.209TyrGlu: 3.209 ± 0.619
1.604TyrPhe: 1.604 ± 0.377
3.02TyrGly: 3.02 ± 0.455
0.283TyrHis: 0.283 ± 0.161
3.209TyrIle: 3.209 ± 0.506
1.604TyrLys: 1.604 ± 0.389
2.737TyrLeu: 2.737 ± 0.479
1.604TyrMet: 1.604 ± 0.353
1.51TyrAsn: 1.51 ± 0.412
1.982TyrPro: 1.982 ± 0.4
1.793TyrGln: 1.793 ± 0.468
1.888TyrArg: 1.888 ± 0.445
1.982TyrSer: 1.982 ± 0.44
2.737TyrThr: 2.737 ± 0.582
2.454TyrVal: 2.454 ± 0.494
0.283TyrTrp: 0.283 ± 0.172
1.888TyrTyr: 1.888 ± 0.392
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (10597 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski