Amino acid dipepetide frequency for Actinomadura sp. RB29

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
24.901AlaAla: 24.901 ± 0.224
1.154AlaCys: 1.154 ± 0.028
9.59AlaAsp: 9.59 ± 0.099
8.953AlaGlu: 8.953 ± 0.097
3.954AlaPhe: 3.954 ± 0.052
14.309AlaGly: 14.309 ± 0.101
2.876AlaHis: 2.876 ± 0.041
3.668AlaIle: 3.668 ± 0.051
2.534AlaLys: 2.534 ± 0.049
16.095AlaLeu: 16.095 ± 0.145
2.347AlaMet: 2.347 ± 0.038
1.903AlaAsn: 1.903 ± 0.038
7.954AlaPro: 7.954 ± 0.097
3.285AlaGln: 3.285 ± 0.047
12.297AlaArg: 12.297 ± 0.106
5.951AlaSer: 5.951 ± 0.062
6.881AlaThr: 6.881 ± 0.066
13.208AlaVal: 13.208 ± 0.12
1.978AlaTrp: 1.978 ± 0.034
2.678AlaTyr: 2.678 ± 0.044
0.0AlaXaa: 0.0 ± 0.0
Cys
1.099CysAla: 1.099 ± 0.025
0.076CysCys: 0.076 ± 0.006
0.481CysAsp: 0.481 ± 0.015
0.331CysGlu: 0.331 ± 0.015
0.213CysPhe: 0.213 ± 0.012
0.898CysGly: 0.898 ± 0.027
0.171CysHis: 0.171 ± 0.01
0.113CysIle: 0.113 ± 0.008
0.116CysLys: 0.116 ± 0.009
0.675CysLeu: 0.675 ± 0.02
0.112CysMet: 0.112 ± 0.009
0.125CysAsn: 0.125 ± 0.008
0.489CysPro: 0.489 ± 0.019
0.131CysGln: 0.131 ± 0.008
0.612CysArg: 0.612 ± 0.019
0.362CysSer: 0.362 ± 0.014
0.411CysThr: 0.411 ± 0.017
0.645CysVal: 0.645 ± 0.019
0.095CysTrp: 0.095 ± 0.007
0.14CysTyr: 0.14 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
9.436AspAla: 9.436 ± 0.09
0.374AspCys: 0.374 ± 0.015
4.25AspAsp: 4.25 ± 0.052
3.871AspGlu: 3.871 ± 0.051
1.476AspPhe: 1.476 ± 0.029
6.958AspGly: 6.958 ± 0.071
1.355AspHis: 1.355 ± 0.031
1.546AspIle: 1.546 ± 0.032
0.927AspLys: 0.927 ± 0.027
7.214AspLeu: 7.214 ± 0.074
0.749AspMet: 0.749 ± 0.019
0.766AspAsn: 0.766 ± 0.023
5.02AspPro: 5.02 ± 0.054
1.35AspGln: 1.35 ± 0.029
5.691AspArg: 5.691 ± 0.064
1.727AspSer: 1.727 ± 0.031
2.664AspThr: 2.664 ± 0.045
5.882AspVal: 5.882 ± 0.063
0.899AspTrp: 0.899 ± 0.019
1.069AspTyr: 1.069 ± 0.025
0.0AspXaa: 0.0 ± 0.0
Glu
6.399GluAla: 6.399 ± 0.076
0.335GluCys: 0.335 ± 0.014
2.652GluAsp: 2.652 ± 0.044
2.771GluGlu: 2.771 ± 0.046
1.47GluPhe: 1.47 ± 0.03
3.314GluGly: 3.314 ± 0.044
1.593GluHis: 1.593 ± 0.03
2.481GluIle: 2.481 ± 0.037
1.166GluLys: 1.166 ± 0.029
6.266GluLeu: 6.266 ± 0.072
0.878GluMet: 0.878 ± 0.025
0.915GluAsn: 0.915 ± 0.022
3.627GluPro: 3.627 ± 0.051
1.778GluGln: 1.778 ± 0.038
5.997GluArg: 5.997 ± 0.074
2.186GluSer: 2.186 ± 0.033
2.865GluThr: 2.865 ± 0.042
3.903GluVal: 3.903 ± 0.058
0.685GluTrp: 0.685 ± 0.02
0.994GluTyr: 0.994 ± 0.023
0.0GluXaa: 0.0 ± 0.0
Phe
4.249PheAla: 4.249 ± 0.054
0.27PheCys: 0.27 ± 0.013
2.07PheAsp: 2.07 ± 0.038
1.347PheGlu: 1.347 ± 0.028
0.861PhePhe: 0.861 ± 0.023
3.219PheGly: 3.219 ± 0.046
0.628PheHis: 0.628 ± 0.018
0.635PheIle: 0.635 ± 0.02
0.445PheLys: 0.445 ± 0.02
2.644PheLeu: 2.644 ± 0.036
0.351PheMet: 0.351 ± 0.015
0.506PheAsn: 0.506 ± 0.016
1.449PhePro: 1.449 ± 0.026
0.676PheGln: 0.676 ± 0.019
1.932PheArg: 1.932 ± 0.033
1.298PheSer: 1.298 ± 0.028
1.958PheThr: 1.958 ± 0.034
2.42PheVal: 2.42 ± 0.036
0.393PheTrp: 0.393 ± 0.015
0.554PheTyr: 0.554 ± 0.017
0.0PheXaa: 0.0 ± 0.0
Gly
11.987GlyAla: 11.987 ± 0.089
0.737GlyCys: 0.737 ± 0.022
5.859GlyAsp: 5.859 ± 0.068
4.775GlyGlu: 4.775 ± 0.058
2.907GlyPhe: 2.907 ± 0.042
9.097GlyGly: 9.097 ± 0.103
2.189GlyHis: 2.189 ± 0.039
3.043GlyIle: 3.043 ± 0.051
2.114GlyLys: 2.114 ± 0.045
9.712GlyLeu: 9.712 ± 0.088
1.877GlyMet: 1.877 ± 0.034
1.541GlyAsn: 1.541 ± 0.037
5.579GlyPro: 5.579 ± 0.067
2.222GlyGln: 2.222 ± 0.046
8.761GlyArg: 8.761 ± 0.091
4.425GlySer: 4.425 ± 0.065
6.383GlyThr: 6.383 ± 0.07
7.93GlyVal: 7.93 ± 0.064
1.597GlyTrp: 1.597 ± 0.029
2.152GlyTyr: 2.152 ± 0.035
0.0GlyXaa: 0.0 ± 0.0
His
2.959HisAla: 2.959 ± 0.051
0.185HisCys: 0.185 ± 0.009
1.362HisAsp: 1.362 ± 0.031
1.107HisGlu: 1.107 ± 0.024
0.55HisPhe: 0.55 ± 0.017
2.383HisGly: 2.383 ± 0.041
0.635HisHis: 0.635 ± 0.024
0.583HisIle: 0.583 ± 0.02
0.26HisLys: 0.26 ± 0.014
2.336HisLeu: 2.336 ± 0.036
0.3HisMet: 0.3 ± 0.012
0.306HisAsn: 0.306 ± 0.013
1.681HisPro: 1.681 ± 0.04
0.469HisGln: 0.469 ± 0.018
2.113HisArg: 2.113 ± 0.039
0.814HisSer: 0.814 ± 0.024
0.983HisThr: 0.983 ± 0.025
1.816HisVal: 1.816 ± 0.032
0.316HisTrp: 0.316 ± 0.013
0.453HisTyr: 0.453 ± 0.016
0.0HisXaa: 0.0 ± 0.0
Ile
4.838IleAla: 4.838 ± 0.059
0.236IleCys: 0.236 ± 0.013
2.202IleAsp: 2.202 ± 0.036
2.034IleGlu: 2.034 ± 0.035
0.711IlePhe: 0.711 ± 0.019
3.406IleGly: 3.406 ± 0.05
0.528IleHis: 0.528 ± 0.016
0.929IleIle: 0.929 ± 0.024
0.661IleLys: 0.661 ± 0.022
2.384IleLeu: 2.384 ± 0.038
0.441IleMet: 0.441 ± 0.016
0.615IleAsn: 0.615 ± 0.019
1.66IlePro: 1.66 ± 0.031
0.602IleGln: 0.602 ± 0.02
2.206IleArg: 2.206 ± 0.04
1.412IleSer: 1.412 ± 0.031
2.104IleThr: 2.104 ± 0.037
3.161IleVal: 3.161 ± 0.049
0.301IleTrp: 0.301 ± 0.013
0.511IleTyr: 0.511 ± 0.015
0.0IleXaa: 0.0 ± 0.0
Lys
2.438LysAla: 2.438 ± 0.047
0.092LysCys: 0.092 ± 0.008
1.1LysAsp: 1.1 ± 0.029
0.912LysGlu: 0.912 ± 0.026
0.435LysPhe: 0.435 ± 0.015
1.451LysGly: 1.451 ± 0.036
0.371LysHis: 0.371 ± 0.013
0.903LysIle: 0.903 ± 0.027
0.674LysLys: 0.674 ± 0.026
1.771LysLeu: 1.771 ± 0.037
0.338LysMet: 0.338 ± 0.015
0.475LysAsn: 0.475 ± 0.018
1.192LysPro: 1.192 ± 0.027
0.516LysGln: 0.516 ± 0.015
1.304LysArg: 1.304 ± 0.032
0.952LysSer: 0.952 ± 0.027
1.159LysThr: 1.159 ± 0.03
1.595LysVal: 1.595 ± 0.038
0.212LysTrp: 0.212 ± 0.011
0.399LysTyr: 0.399 ± 0.016
0.0LysXaa: 0.0 ± 0.0
Leu
17.119LeuAla: 17.119 ± 0.155
0.762LeuCys: 0.762 ± 0.02
7.483LeuAsp: 7.483 ± 0.082
4.484LeuGlu: 4.484 ± 0.053
2.766LeuPhe: 2.766 ± 0.047
9.467LeuGly: 9.467 ± 0.087
2.189LeuHis: 2.189 ± 0.044
3.067LeuIle: 3.067 ± 0.044
1.691LeuLys: 1.691 ± 0.037
11.23LeuLeu: 11.23 ± 0.111
1.437LeuMet: 1.437 ± 0.027
1.719LeuAsn: 1.719 ± 0.032
6.73LeuPro: 6.73 ± 0.056
1.862LeuGln: 1.862 ± 0.036
9.584LeuArg: 9.584 ± 0.084
4.689LeuSer: 4.689 ± 0.058
6.788LeuThr: 6.788 ± 0.073
9.107LeuVal: 9.107 ± 0.088
1.265LeuTrp: 1.265 ± 0.027
1.736LeuTyr: 1.736 ± 0.028
0.0LeuXaa: 0.0 ± 0.0
Met
2.052MetAla: 2.052 ± 0.034
0.112MetCys: 0.112 ± 0.008
0.861MetAsp: 0.861 ± 0.02
0.66MetGlu: 0.66 ± 0.021
0.476MetPhe: 0.476 ± 0.017
1.132MetGly: 1.132 ± 0.024
0.3MetHis: 0.3 ± 0.014
0.701MetIle: 0.701 ± 0.022
0.383MetLys: 0.383 ± 0.017
1.581MetLeu: 1.581 ± 0.032
0.274MetMet: 0.274 ± 0.013
0.428MetAsn: 0.428 ± 0.015
1.037MetPro: 1.037 ± 0.025
0.338MetGln: 0.338 ± 0.013
1.449MetArg: 1.449 ± 0.027
1.166MetSer: 1.166 ± 0.026
1.504MetThr: 1.504 ± 0.027
1.197MetVal: 1.197 ± 0.027
0.17MetTrp: 0.17 ± 0.01
0.258MetTyr: 0.258 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
2.283AsnAla: 2.283 ± 0.04
0.152AsnCys: 0.152 ± 0.009
0.91AsnAsp: 0.91 ± 0.022
0.705AsnGlu: 0.705 ± 0.022
0.416AsnPhe: 0.416 ± 0.016
1.749AsnGly: 1.749 ± 0.042
0.321AsnHis: 0.321 ± 0.014
0.578AsnIle: 0.578 ± 0.019
0.332AsnLys: 0.332 ± 0.014
1.614AsnLeu: 1.614 ± 0.028
0.254AsnMet: 0.254 ± 0.01
0.351AsnAsn: 0.351 ± 0.016
1.258AsnPro: 1.258 ± 0.026
0.425AsnGln: 0.425 ± 0.016
1.26AsnArg: 1.26 ± 0.026
0.688AsnSer: 0.688 ± 0.022
1.001AsnThr: 1.001 ± 0.027
1.457AsnVal: 1.457 ± 0.031
0.229AsnTrp: 0.229 ± 0.012
0.389AsnTyr: 0.389 ± 0.015
0.0AsnXaa: 0.0 ± 0.0
Pro
9.574ProAla: 9.574 ± 0.114
0.335ProCys: 0.335 ± 0.013
5.228ProAsp: 5.228 ± 0.057
3.991ProGlu: 3.991 ± 0.053
1.735ProPhe: 1.735 ± 0.029
7.562ProGly: 7.562 ± 0.089
1.339ProHis: 1.339 ± 0.03
1.485ProIle: 1.485 ± 0.03
1.146ProLys: 1.146 ± 0.027
5.613ProLeu: 5.613 ± 0.063
0.94ProMet: 0.94 ± 0.022
0.942ProAsn: 0.942 ± 0.027
4.645ProPro: 4.645 ± 0.097
1.435ProGln: 1.435 ± 0.035
4.548ProArg: 4.548 ± 0.056
3.496ProSer: 3.496 ± 0.056
2.754ProThr: 2.754 ± 0.04
5.781ProVal: 5.781 ± 0.059
0.934ProTrp: 0.934 ± 0.025
1.218ProTyr: 1.218 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
3.17GlnAla: 3.17 ± 0.053
0.145GlnCys: 0.145 ± 0.009
1.138GlnAsp: 1.138 ± 0.026
1.116GlnGlu: 1.116 ± 0.029
0.665GlnPhe: 0.665 ± 0.019
1.859GlnGly: 1.859 ± 0.041
0.51GlnHis: 0.51 ± 0.018
1.072GlnIle: 1.072 ± 0.023
0.512GlnLys: 0.512 ± 0.017
2.214GlnLeu: 2.214 ± 0.036
0.402GlnMet: 0.402 ± 0.016
0.48GlnAsn: 0.48 ± 0.016
1.376GlnPro: 1.376 ± 0.034
0.963GlnGln: 0.963 ± 0.04
2.215GlnArg: 2.215 ± 0.035
0.968GlnSer: 0.968 ± 0.024
1.117GlnThr: 1.117 ± 0.025
2.17GlnVal: 2.17 ± 0.04
0.363GlnTrp: 0.363 ± 0.015
0.476GlnTyr: 0.476 ± 0.016
0.0GlnXaa: 0.0 ± 0.0
Arg
12.104ArgAla: 12.104 ± 0.12
0.594ArgCys: 0.594 ± 0.018
5.443ArgAsp: 5.443 ± 0.063
4.581ArgGlu: 4.581 ± 0.06
2.588ArgPhe: 2.588 ± 0.037
6.205ArgGly: 6.205 ± 0.057
2.179ArgHis: 2.179 ± 0.034
3.193ArgIle: 3.193 ± 0.048
1.439ArgLys: 1.439 ± 0.032
9.794ArgLeu: 9.794 ± 0.08
1.825ArgMet: 1.825 ± 0.034
1.347ArgAsn: 1.347 ± 0.028
6.036ArgPro: 6.036 ± 0.063
2.008ArgGln: 2.008 ± 0.035
9.278ArgArg: 9.278 ± 0.105
3.878ArgSer: 3.878 ± 0.05
5.567ArgThr: 5.567 ± 0.06
6.902ArgVal: 6.902 ± 0.068
1.409ArgTrp: 1.409 ± 0.029
1.82ArgTyr: 1.82 ± 0.035
0.0ArgXaa: 0.0 ± 0.0
Ser
6.27SerAla: 6.27 ± 0.063
0.332SerCys: 0.332 ± 0.014
2.361SerAsp: 2.361 ± 0.04
1.851SerGlu: 1.851 ± 0.033
1.406SerPhe: 1.406 ± 0.032
5.593SerGly: 5.593 ± 0.067
0.822SerHis: 0.822 ± 0.021
1.363SerIle: 1.363 ± 0.031
0.745SerLys: 0.745 ± 0.024
4.385SerLeu: 4.385 ± 0.058
0.932SerMet: 0.932 ± 0.023
0.713SerAsn: 0.713 ± 0.022
3.001SerPro: 3.001 ± 0.05
0.913SerGln: 0.913 ± 0.024
3.486SerArg: 3.486 ± 0.048
2.281SerSer: 2.281 ± 0.043
2.373SerThr: 2.373 ± 0.045
3.839SerVal: 3.839 ± 0.05
0.767SerTrp: 0.767 ± 0.021
1.045SerTyr: 1.045 ± 0.03
0.0SerXaa: 0.0 ± 0.0
Thr
8.796ThrAla: 8.796 ± 0.088
0.405ThrCys: 0.405 ± 0.015
3.189ThrAsp: 3.189 ± 0.041
2.664ThrGlu: 2.664 ± 0.047
1.7ThrPhe: 1.7 ± 0.03
6.756ThrGly: 6.756 ± 0.08
0.981ThrHis: 0.981 ± 0.024
1.848ThrIle: 1.848 ± 0.032
0.945ThrLys: 0.945 ± 0.028
5.65ThrLeu: 5.65 ± 0.063
0.864ThrMet: 0.864 ± 0.021
0.903ThrAsn: 0.903 ± 0.026
3.932ThrPro: 3.932 ± 0.056
1.01ThrGln: 1.01 ± 0.023
4.113ThrArg: 4.113 ± 0.047
2.85ThrSer: 2.85 ± 0.047
3.405ThrThr: 3.405 ± 0.061
5.874ThrVal: 5.874 ± 0.072
0.858ThrTrp: 0.858 ± 0.023
1.156ThrTyr: 1.156 ± 0.028
0.0ThrXaa: 0.0 ± 0.0
Val
12.634ValAla: 12.634 ± 0.094
0.688ValCys: 0.688 ± 0.021
4.946ValAsp: 4.946 ± 0.054
4.427ValGlu: 4.427 ± 0.057
2.508ValPhe: 2.508 ± 0.036
6.479ValGly: 6.479 ± 0.071
1.904ValHis: 1.904 ± 0.033
2.737ValIle: 2.737 ± 0.036
1.556ValLys: 1.556 ± 0.037
10.288ValLeu: 10.288 ± 0.102
1.267ValMet: 1.267 ± 0.027
1.621ValAsn: 1.621 ± 0.033
6.158ValPro: 6.158 ± 0.066
1.995ValGln: 1.995 ± 0.035
8.084ValArg: 8.084 ± 0.082
3.742ValSer: 3.742 ± 0.047
5.67ValThr: 5.67 ± 0.083
8.365ValVal: 8.365 ± 0.085
1.119ValTrp: 1.119 ± 0.028
1.529ValTyr: 1.529 ± 0.028
0.0ValXaa: 0.0 ± 0.0
Trp
1.573TrpAla: 1.573 ± 0.031
0.14TrpCys: 0.14 ± 0.009
0.795TrpAsp: 0.795 ± 0.022
0.673TrpGlu: 0.673 ± 0.021
0.462TrpPhe: 0.462 ± 0.015
0.983TrpGly: 0.983 ± 0.03
0.343TrpHis: 0.343 ± 0.016
0.543TrpIle: 0.543 ± 0.017
0.31TrpLys: 0.31 ± 0.014
1.643TrpLeu: 1.643 ± 0.028
0.259TrpMet: 0.259 ± 0.011
0.368TrpAsn: 0.368 ± 0.015
0.837TrpPro: 0.837 ± 0.023
0.414TrpGln: 0.414 ± 0.014
1.451TrpArg: 1.451 ± 0.029
0.808TrpSer: 0.808 ± 0.021
1.178TrpThr: 1.178 ± 0.028
0.837TrpVal: 0.837 ± 0.023
0.319TrpTrp: 0.319 ± 0.014
0.299TrpTyr: 0.299 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.633TyrAla: 2.633 ± 0.042
0.172TyrCys: 0.172 ± 0.01
1.305TyrAsp: 1.305 ± 0.026
1.02TyrGlu: 1.02 ± 0.025
0.608TyrPhe: 0.608 ± 0.019
2.066TyrGly: 2.066 ± 0.041
0.378TyrHis: 0.378 ± 0.012
0.452TyrIle: 0.452 ± 0.017
0.338TyrLys: 0.338 ± 0.016
2.066TyrLeu: 2.066 ± 0.034
0.233TyrMet: 0.233 ± 0.012
0.344TyrAsn: 0.344 ± 0.015
1.036TyrPro: 1.036 ± 0.024
0.539TyrGln: 0.539 ± 0.017
1.811TyrArg: 1.811 ± 0.036
0.785TyrSer: 0.785 ± 0.022
1.135TyrThr: 1.135 ± 0.034
1.627TyrVal: 1.627 ± 0.032
0.329TyrTrp: 0.329 ± 0.012
0.397TyrTyr: 0.397 ± 0.016
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5700 proteins (1924993 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski