Amino acid dipepetide frequency for Microbacterium phage Eden

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.778AlaAla: 12.778 ± 1.066
0.316AlaCys: 0.316 ± 0.167
7.967AlaAsp: 7.967 ± 0.831
7.02AlaGlu: 7.02 ± 0.827
3.707AlaPhe: 3.707 ± 0.573
10.096AlaGly: 10.096 ± 0.897
1.893AlaHis: 1.893 ± 0.41
6.389AlaIle: 6.389 ± 0.792
6.152AlaLys: 6.152 ± 0.78
10.017AlaLeu: 10.017 ± 0.809
2.13AlaMet: 2.13 ± 0.421
4.023AlaAsn: 4.023 ± 0.72
4.417AlaPro: 4.417 ± 0.689
5.206AlaGln: 5.206 ± 0.462
5.364AlaArg: 5.364 ± 0.625
6.074AlaSer: 6.074 ± 0.817
6.705AlaThr: 6.705 ± 0.757
7.572AlaVal: 7.572 ± 0.784
2.13AlaTrp: 2.13 ± 0.457
2.603AlaTyr: 2.603 ± 0.636
0.0AlaXaa: 0.0 ± 0.0
Cys
0.237CysAla: 0.237 ± 0.138
0.0CysCys: 0.0 ± 0.0
0.316CysAsp: 0.316 ± 0.164
0.079CysGlu: 0.079 ± 0.068
0.237CysPhe: 0.237 ± 0.12
0.631CysGly: 0.631 ± 0.207
0.237CysHis: 0.237 ± 0.13
0.158CysIle: 0.158 ± 0.102
0.394CysLys: 0.394 ± 0.17
0.237CysLeu: 0.237 ± 0.146
0.158CysMet: 0.158 ± 0.119
0.158CysAsn: 0.158 ± 0.095
0.552CysPro: 0.552 ± 0.198
0.079CysGln: 0.079 ± 0.074
0.316CysArg: 0.316 ± 0.15
0.394CysSer: 0.394 ± 0.191
0.316CysThr: 0.316 ± 0.146
0.158CysVal: 0.158 ± 0.109
0.0CysTrp: 0.0 ± 0.0
0.158CysTyr: 0.158 ± 0.112
0.0CysXaa: 0.0 ± 0.0
Asp
7.178AspAla: 7.178 ± 0.715
0.158AspCys: 0.158 ± 0.11
3.944AspAsp: 3.944 ± 0.583
4.89AspGlu: 4.89 ± 0.588
2.682AspPhe: 2.682 ± 0.382
7.414AspGly: 7.414 ± 0.765
1.814AspHis: 1.814 ± 0.376
2.366AspIle: 2.366 ± 0.453
1.183AspLys: 1.183 ± 0.292
7.414AspLeu: 7.414 ± 0.926
1.578AspMet: 1.578 ± 0.303
1.893AspAsn: 1.893 ± 0.344
3.549AspPro: 3.549 ± 0.589
1.735AspGln: 1.735 ± 0.31
3.155AspArg: 3.155 ± 0.555
3.786AspSer: 3.786 ± 0.515
2.84AspThr: 2.84 ± 0.45
3.865AspVal: 3.865 ± 0.664
0.947AspTrp: 0.947 ± 0.268
1.972AspTyr: 1.972 ± 0.389
0.0AspXaa: 0.0 ± 0.0
Glu
6.626GluAla: 6.626 ± 0.728
0.158GluCys: 0.158 ± 0.1
5.285GluAsp: 5.285 ± 0.735
5.048GluGlu: 5.048 ± 0.672
2.997GluPhe: 2.997 ± 0.458
3.865GluGly: 3.865 ± 0.493
1.42GluHis: 1.42 ± 0.287
1.183GluIle: 1.183 ± 0.358
2.13GluLys: 2.13 ± 0.441
7.336GluLeu: 7.336 ± 0.991
1.341GluMet: 1.341 ± 0.268
1.578GluAsn: 1.578 ± 0.384
2.603GluPro: 2.603 ± 0.355
2.209GluGln: 2.209 ± 0.46
3.234GluArg: 3.234 ± 0.557
2.682GluSer: 2.682 ± 0.474
4.18GluThr: 4.18 ± 0.523
5.679GluVal: 5.679 ± 0.67
2.287GluTrp: 2.287 ± 0.405
1.262GluTyr: 1.262 ± 0.296
0.0GluXaa: 0.0 ± 0.0
Phe
3.155PheAla: 3.155 ± 0.501
0.237PheCys: 0.237 ± 0.128
2.287PheAsp: 2.287 ± 0.41
2.366PheGlu: 2.366 ± 0.342
0.868PhePhe: 0.868 ± 0.237
2.761PheGly: 2.761 ± 0.621
0.552PheHis: 0.552 ± 0.171
2.13PheIle: 2.13 ± 0.481
1.735PheLys: 1.735 ± 0.36
2.761PheLeu: 2.761 ± 0.51
0.71PheMet: 0.71 ± 0.28
1.42PheAsn: 1.42 ± 0.316
1.262PhePro: 1.262 ± 0.291
1.104PheGln: 1.104 ± 0.341
3.076PheArg: 3.076 ± 0.439
2.209PheSer: 2.209 ± 0.373
2.209PheThr: 2.209 ± 0.388
2.051PheVal: 2.051 ± 0.494
0.394PheTrp: 0.394 ± 0.246
0.789PheTyr: 0.789 ± 0.216
0.0PheXaa: 0.0 ± 0.0
Gly
9.229GlyAla: 9.229 ± 0.815
0.552GlyCys: 0.552 ± 0.252
5.127GlyAsp: 5.127 ± 0.693
4.89GlyGlu: 4.89 ± 0.635
3.155GlyPhe: 3.155 ± 0.83
5.679GlyGly: 5.679 ± 0.836
1.262GlyHis: 1.262 ± 0.309
3.786GlyIle: 3.786 ± 0.899
3.707GlyLys: 3.707 ± 0.573
6.547GlyLeu: 6.547 ± 0.678
2.287GlyMet: 2.287 ± 0.424
2.761GlyAsn: 2.761 ± 0.553
3.076GlyPro: 3.076 ± 0.467
3.549GlyGln: 3.549 ± 0.83
4.811GlyArg: 4.811 ± 0.577
4.102GlySer: 4.102 ± 0.62
5.521GlyThr: 5.521 ± 0.563
6.152GlyVal: 6.152 ± 0.825
2.603GlyTrp: 2.603 ± 0.485
3.076GlyTyr: 3.076 ± 0.549
0.0GlyXaa: 0.0 ± 0.0
His
1.893HisAla: 1.893 ± 0.433
0.237HisCys: 0.237 ± 0.123
1.578HisAsp: 1.578 ± 0.332
1.025HisGlu: 1.025 ± 0.286
1.104HisPhe: 1.104 ± 0.258
1.735HisGly: 1.735 ± 0.364
0.316HisHis: 0.316 ± 0.143
0.789HisIle: 0.789 ± 0.329
0.552HisLys: 0.552 ± 0.225
2.051HisLeu: 2.051 ± 0.43
0.552HisMet: 0.552 ± 0.158
0.789HisAsn: 0.789 ± 0.221
1.025HisPro: 1.025 ± 0.276
0.631HisGln: 0.631 ± 0.21
0.631HisArg: 0.631 ± 0.238
0.71HisSer: 0.71 ± 0.255
0.947HisThr: 0.947 ± 0.26
1.262HisVal: 1.262 ± 0.296
0.552HisTrp: 0.552 ± 0.222
0.552HisTyr: 0.552 ± 0.205
0.0HisXaa: 0.0 ± 0.0
Ile
5.442IleAla: 5.442 ± 0.616
0.079IleCys: 0.079 ± 0.078
2.918IleAsp: 2.918 ± 0.359
3.628IleGlu: 3.628 ± 0.553
0.789IlePhe: 0.789 ± 0.275
3.549IleGly: 3.549 ± 0.598
1.341IleHis: 1.341 ± 0.413
2.366IleIle: 2.366 ± 0.455
2.445IleLys: 2.445 ± 0.457
3.392IleLeu: 3.392 ± 0.447
0.947IleMet: 0.947 ± 0.329
1.972IleAsn: 1.972 ± 0.465
1.893IlePro: 1.893 ± 0.328
1.578IleGln: 1.578 ± 0.337
3.313IleArg: 3.313 ± 0.54
1.814IleSer: 1.814 ± 0.406
3.707IleThr: 3.707 ± 0.517
3.786IleVal: 3.786 ± 0.634
0.552IleTrp: 0.552 ± 0.242
0.947IleTyr: 0.947 ± 0.339
0.0IleXaa: 0.0 ± 0.0
Lys
6.074LysAla: 6.074 ± 0.927
0.473LysCys: 0.473 ± 0.183
2.603LysAsp: 2.603 ± 0.45
1.735LysGlu: 1.735 ± 0.364
1.578LysPhe: 1.578 ± 0.354
3.628LysGly: 3.628 ± 0.615
0.789LysHis: 0.789 ± 0.282
1.499LysIle: 1.499 ± 0.361
2.209LysLys: 2.209 ± 0.452
3.944LysLeu: 3.944 ± 0.507
1.183LysMet: 1.183 ± 0.261
1.656LysAsn: 1.656 ± 0.404
2.603LysPro: 2.603 ± 0.492
1.42LysGln: 1.42 ± 0.352
3.392LysArg: 3.392 ± 0.546
3.155LysSer: 3.155 ± 0.582
3.076LysThr: 3.076 ± 0.585
2.13LysVal: 2.13 ± 0.366
0.631LysTrp: 0.631 ± 0.209
1.025LysTyr: 1.025 ± 0.265
0.0LysXaa: 0.0 ± 0.0
Leu
9.702LeuAla: 9.702 ± 0.885
0.552LeuCys: 0.552 ± 0.202
5.995LeuAsp: 5.995 ± 0.727
5.048LeuGlu: 5.048 ± 0.694
2.918LeuPhe: 2.918 ± 0.347
6.468LeuGly: 6.468 ± 0.69
0.947LeuHis: 0.947 ± 0.303
3.865LeuIle: 3.865 ± 0.431
4.18LeuLys: 4.18 ± 0.555
6.783LeuLeu: 6.783 ± 0.739
2.682LeuMet: 2.682 ± 0.332
2.997LeuAsn: 2.997 ± 0.553
5.206LeuPro: 5.206 ± 0.521
3.076LeuGln: 3.076 ± 0.535
5.442LeuArg: 5.442 ± 0.668
4.259LeuSer: 4.259 ± 0.584
6.389LeuThr: 6.389 ± 0.601
6.547LeuVal: 6.547 ± 0.811
1.893LeuTrp: 1.893 ± 0.427
1.341LeuTyr: 1.341 ± 0.316
0.0LeuXaa: 0.0 ± 0.0
Met
3.076MetAla: 3.076 ± 0.423
0.0MetCys: 0.0 ± 0.0
1.42MetAsp: 1.42 ± 0.338
0.947MetGlu: 0.947 ± 0.238
0.158MetPhe: 0.158 ± 0.101
1.578MetGly: 1.578 ± 0.301
0.158MetHis: 0.158 ± 0.117
0.552MetIle: 0.552 ± 0.212
1.341MetLys: 1.341 ± 0.391
1.656MetLeu: 1.656 ± 0.373
0.237MetMet: 0.237 ± 0.151
1.42MetAsn: 1.42 ± 0.352
0.947MetPro: 0.947 ± 0.287
0.631MetGln: 0.631 ± 0.27
1.735MetArg: 1.735 ± 0.415
0.947MetSer: 0.947 ± 0.207
2.603MetThr: 2.603 ± 0.496
1.893MetVal: 1.893 ± 0.354
0.552MetTrp: 0.552 ± 0.178
0.631MetTyr: 0.631 ± 0.221
0.0MetXaa: 0.0 ± 0.0
Asn
3.471AsnAla: 3.471 ± 0.6
0.158AsnCys: 0.158 ± 0.097
1.262AsnAsp: 1.262 ± 0.262
2.287AsnGlu: 2.287 ± 0.383
0.947AsnPhe: 0.947 ± 0.253
4.654AsnGly: 4.654 ± 0.776
0.71AsnHis: 0.71 ± 0.247
1.578AsnIle: 1.578 ± 0.393
1.972AsnLys: 1.972 ± 0.4
2.13AsnLeu: 2.13 ± 0.441
0.71AsnMet: 0.71 ± 0.209
1.025AsnAsn: 1.025 ± 0.256
2.287AsnPro: 2.287 ± 0.414
1.025AsnGln: 1.025 ± 0.285
2.997AsnArg: 2.997 ± 0.498
2.84AsnSer: 2.84 ± 0.531
2.051AsnThr: 2.051 ± 0.513
1.814AsnVal: 1.814 ± 0.434
0.631AsnTrp: 0.631 ± 0.237
1.183AsnTyr: 1.183 ± 0.294
0.0AsnXaa: 0.0 ± 0.0
Pro
6.074ProAla: 6.074 ± 0.577
0.394ProCys: 0.394 ± 0.198
3.313ProAsp: 3.313 ± 0.497
4.969ProGlu: 4.969 ± 0.674
1.893ProPhe: 1.893 ± 0.366
3.549ProGly: 3.549 ± 0.537
0.868ProHis: 0.868 ± 0.252
2.524ProIle: 2.524 ± 0.451
2.84ProLys: 2.84 ± 0.557
2.918ProLeu: 2.918 ± 0.558
0.631ProMet: 0.631 ± 0.212
1.656ProAsn: 1.656 ± 0.269
1.183ProPro: 1.183 ± 0.254
1.262ProGln: 1.262 ± 0.27
2.287ProArg: 2.287 ± 0.382
3.392ProSer: 3.392 ± 0.471
2.918ProThr: 2.918 ± 0.527
3.392ProVal: 3.392 ± 0.509
0.947ProTrp: 0.947 ± 0.25
1.025ProTyr: 1.025 ± 0.265
0.0ProXaa: 0.0 ± 0.0
Gln
5.442GlnAla: 5.442 ± 0.674
0.237GlnCys: 0.237 ± 0.122
1.578GlnAsp: 1.578 ± 0.314
2.209GlnGlu: 2.209 ± 0.537
1.656GlnPhe: 1.656 ± 0.314
2.287GlnGly: 2.287 ± 0.414
0.631GlnHis: 0.631 ± 0.231
1.025GlnIle: 1.025 ± 0.249
0.789GlnLys: 0.789 ± 0.2
3.944GlnLeu: 3.944 ± 0.596
0.71GlnMet: 0.71 ± 0.196
1.893GlnAsn: 1.893 ± 0.354
1.735GlnPro: 1.735 ± 0.374
1.025GlnGln: 1.025 ± 0.285
1.814GlnArg: 1.814 ± 0.254
1.656GlnSer: 1.656 ± 0.338
1.578GlnThr: 1.578 ± 0.362
3.944GlnVal: 3.944 ± 0.522
0.631GlnTrp: 0.631 ± 0.19
0.631GlnTyr: 0.631 ± 0.249
0.0GlnXaa: 0.0 ± 0.0
Arg
5.521ArgAla: 5.521 ± 0.734
0.237ArgCys: 0.237 ± 0.183
3.628ArgAsp: 3.628 ± 0.587
3.944ArgGlu: 3.944 ± 0.669
1.656ArgPhe: 1.656 ± 0.347
4.969ArgGly: 4.969 ± 0.622
1.104ArgHis: 1.104 ± 0.296
3.076ArgIle: 3.076 ± 0.445
2.761ArgLys: 2.761 ± 0.59
5.285ArgLeu: 5.285 ± 0.741
1.183ArgMet: 1.183 ± 0.366
2.287ArgAsn: 2.287 ± 0.528
3.471ArgPro: 3.471 ± 0.558
1.814ArgGln: 1.814 ± 0.348
5.127ArgArg: 5.127 ± 0.703
3.234ArgSer: 3.234 ± 0.464
3.786ArgThr: 3.786 ± 0.458
4.89ArgVal: 4.89 ± 0.536
1.499ArgTrp: 1.499 ± 0.356
1.42ArgTyr: 1.42 ± 0.278
0.0ArgXaa: 0.0 ± 0.0
Ser
6.231SerAla: 6.231 ± 0.767
0.237SerCys: 0.237 ± 0.15
3.155SerAsp: 3.155 ± 0.468
2.524SerGlu: 2.524 ± 0.493
1.735SerPhe: 1.735 ± 0.374
5.127SerGly: 5.127 ± 0.62
0.947SerHis: 0.947 ± 0.294
2.13SerIle: 2.13 ± 0.438
2.84SerLys: 2.84 ± 0.52
4.969SerLeu: 4.969 ± 0.636
1.104SerMet: 1.104 ± 0.265
2.366SerAsn: 2.366 ± 0.581
2.84SerPro: 2.84 ± 0.48
2.051SerGln: 2.051 ± 0.397
3.471SerArg: 3.471 ± 0.585
4.338SerSer: 4.338 ± 0.778
3.944SerThr: 3.944 ± 0.369
3.313SerVal: 3.313 ± 0.518
1.814SerTrp: 1.814 ± 0.371
0.789SerTyr: 0.789 ± 0.257
0.0SerXaa: 0.0 ± 0.0
Thr
8.045ThrAla: 8.045 ± 0.778
0.158ThrCys: 0.158 ± 0.118
4.023ThrAsp: 4.023 ± 0.562
3.471ThrGlu: 3.471 ± 0.469
2.682ThrPhe: 2.682 ± 0.414
5.442ThrGly: 5.442 ± 0.825
1.656ThrHis: 1.656 ± 0.393
4.496ThrIle: 4.496 ± 0.64
3.392ThrLys: 3.392 ± 0.432
5.442ThrLeu: 5.442 ± 0.629
1.262ThrMet: 1.262 ± 0.288
1.183ThrAsn: 1.183 ± 0.314
3.471ThrPro: 3.471 ± 0.512
1.893ThrGln: 1.893 ± 0.394
4.18ThrArg: 4.18 ± 0.7
3.471ThrSer: 3.471 ± 0.597
5.364ThrThr: 5.364 ± 0.939
5.127ThrVal: 5.127 ± 0.668
1.025ThrTrp: 1.025 ± 0.302
1.262ThrTyr: 1.262 ± 0.32
0.0ThrXaa: 0.0 ± 0.0
Val
8.282ValAla: 8.282 ± 0.651
0.158ValCys: 0.158 ± 0.106
4.575ValAsp: 4.575 ± 0.559
4.338ValGlu: 4.338 ± 0.595
1.972ValPhe: 1.972 ± 0.396
4.733ValGly: 4.733 ± 0.745
1.578ValHis: 1.578 ± 0.312
3.865ValIle: 3.865 ± 0.55
2.761ValLys: 2.761 ± 0.484
5.995ValLeu: 5.995 ± 0.64
1.735ValMet: 1.735 ± 0.334
2.761ValAsn: 2.761 ± 0.465
4.023ValPro: 4.023 ± 0.576
3.076ValGln: 3.076 ± 0.42
3.786ValArg: 3.786 ± 0.612
3.786ValSer: 3.786 ± 0.512
5.679ValThr: 5.679 ± 0.649
5.048ValVal: 5.048 ± 0.566
1.656ValTrp: 1.656 ± 0.611
1.814ValTyr: 1.814 ± 0.4
0.0ValXaa: 0.0 ± 0.0
Trp
2.209TrpAla: 2.209 ± 0.417
0.158TrpCys: 0.158 ± 0.117
1.025TrpAsp: 1.025 ± 0.283
1.341TrpGlu: 1.341 ± 0.303
0.868TrpPhe: 0.868 ± 0.237
1.578TrpGly: 1.578 ± 0.312
0.158TrpHis: 0.158 ± 0.101
1.42TrpIle: 1.42 ± 0.431
0.868TrpLys: 0.868 ± 0.22
2.287TrpLeu: 2.287 ± 0.401
0.473TrpMet: 0.473 ± 0.146
1.104TrpAsn: 1.104 ± 0.416
0.631TrpPro: 0.631 ± 0.221
1.104TrpGln: 1.104 ± 0.356
0.789TrpArg: 0.789 ± 0.266
1.735TrpSer: 1.735 ± 0.345
1.341TrpThr: 1.341 ± 0.338
1.735TrpVal: 1.735 ± 0.228
0.473TrpTrp: 0.473 ± 0.244
0.631TrpTyr: 0.631 ± 0.177
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.893TyrAla: 1.893 ± 0.361
0.316TyrCys: 0.316 ± 0.156
2.366TyrAsp: 2.366 ± 0.374
1.341TyrGlu: 1.341 ± 0.304
0.552TyrPhe: 0.552 ± 0.197
2.13TyrGly: 2.13 ± 0.504
0.552TyrHis: 0.552 ± 0.163
1.262TyrIle: 1.262 ± 0.339
0.552TyrLys: 0.552 ± 0.227
1.183TyrLeu: 1.183 ± 0.305
0.789TyrMet: 0.789 ± 0.199
0.789TyrAsn: 0.789 ± 0.242
1.262TyrPro: 1.262 ± 0.292
0.947TyrGln: 0.947 ± 0.259
1.972TyrArg: 1.972 ± 0.35
1.341TyrSer: 1.341 ± 0.424
1.893TyrThr: 1.893 ± 0.268
1.262TyrVal: 1.262 ± 0.287
0.71TyrTrp: 0.71 ± 0.276
0.552TyrTyr: 0.552 ± 0.268
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 69 proteins (12679 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski