Amino acid dipepetide frequency for Lactobacillus phage phiAQ113

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.074AlaAla: 4.074 ± 1.196
0.0AlaCys: 0.0 ± 0.0
5.0AlaAsp: 5.0 ± 0.711
4.537AlaGlu: 4.537 ± 0.688
1.759AlaPhe: 1.759 ± 0.36
4.351AlaGly: 4.351 ± 0.49
0.741AlaHis: 0.741 ± 0.258
3.889AlaIle: 3.889 ± 0.516
7.962AlaLys: 7.962 ± 0.822
4.444AlaLeu: 4.444 ± 0.766
2.222AlaMet: 2.222 ± 0.372
5.648AlaAsn: 5.648 ± 0.739
2.685AlaPro: 2.685 ± 0.722
4.074AlaGln: 4.074 ± 0.52
3.055AlaArg: 3.055 ± 0.483
3.981AlaSer: 3.981 ± 0.593
4.537AlaThr: 4.537 ± 0.687
5.185AlaVal: 5.185 ± 0.78
0.833AlaTrp: 0.833 ± 0.343
2.778AlaTyr: 2.778 ± 0.535
0.0AlaXaa: 0.0 ± 0.0
Cys
0.278CysAla: 0.278 ± 0.175
0.185CysCys: 0.185 ± 0.128
0.648CysAsp: 0.648 ± 0.266
0.37CysGlu: 0.37 ± 0.201
0.278CysPhe: 0.278 ± 0.164
0.185CysGly: 0.185 ± 0.12
0.0CysHis: 0.0 ± 0.0
0.463CysIle: 0.463 ± 0.189
1.018CysLys: 1.018 ± 0.331
0.37CysLeu: 0.37 ± 0.23
0.0CysMet: 0.0 ± 0.0
0.278CysAsn: 0.278 ± 0.154
0.278CysPro: 0.278 ± 0.154
0.185CysGln: 0.185 ± 0.125
0.093CysArg: 0.093 ± 0.095
0.741CysSer: 0.741 ± 0.28
0.37CysThr: 0.37 ± 0.174
0.37CysVal: 0.37 ± 0.201
0.093CysTrp: 0.093 ± 0.104
0.556CysTyr: 0.556 ± 0.221
0.0CysXaa: 0.0 ± 0.0
Asp
5.185AspAla: 5.185 ± 0.706
0.37AspCys: 0.37 ± 0.187
5.092AspAsp: 5.092 ± 0.914
5.185AspGlu: 5.185 ± 0.749
3.426AspPhe: 3.426 ± 0.552
4.074AspGly: 4.074 ± 0.562
0.833AspHis: 0.833 ± 0.274
4.166AspIle: 4.166 ± 0.749
4.814AspLys: 4.814 ± 0.57
6.203AspLeu: 6.203 ± 0.768
1.759AspMet: 1.759 ± 0.323
3.796AspAsn: 3.796 ± 0.465
2.037AspPro: 2.037 ± 0.485
2.778AspGln: 2.778 ± 0.488
1.944AspArg: 1.944 ± 0.42
3.611AspSer: 3.611 ± 0.441
2.87AspThr: 2.87 ± 0.446
3.426AspVal: 3.426 ± 0.444
1.018AspTrp: 1.018 ± 0.28
4.259AspTyr: 4.259 ± 0.685
0.093AspXaa: 0.093 ± 0.095
Glu
4.537GluAla: 4.537 ± 0.543
0.278GluCys: 0.278 ± 0.16
4.074GluAsp: 4.074 ± 0.655
3.426GluGlu: 3.426 ± 0.635
2.592GluPhe: 2.592 ± 0.628
2.778GluGly: 2.778 ± 0.443
1.111GluHis: 1.111 ± 0.284
4.074GluIle: 4.074 ± 0.668
4.166GluLys: 4.166 ± 0.57
6.296GluLeu: 6.296 ± 0.802
2.037GluMet: 2.037 ± 0.383
3.981GluAsn: 3.981 ± 0.747
1.389GluPro: 1.389 ± 0.404
2.129GluGln: 2.129 ± 0.476
2.222GluArg: 2.222 ± 0.493
3.796GluSer: 3.796 ± 0.62
3.703GluThr: 3.703 ± 0.511
4.814GluVal: 4.814 ± 0.717
0.833GluTrp: 0.833 ± 0.359
1.759GluTyr: 1.759 ± 0.536
0.0GluXaa: 0.0 ± 0.0
Phe
2.129PheAla: 2.129 ± 0.444
0.278PheCys: 0.278 ± 0.208
3.889PheAsp: 3.889 ± 0.646
2.778PheGlu: 2.778 ± 0.466
0.833PhePhe: 0.833 ± 0.272
1.759PheGly: 1.759 ± 0.411
0.463PheHis: 0.463 ± 0.175
1.944PheIle: 1.944 ± 0.372
3.518PheLys: 3.518 ± 0.705
2.778PheLeu: 2.778 ± 0.435
1.018PheMet: 1.018 ± 0.429
2.407PheAsn: 2.407 ± 0.483
1.111PhePro: 1.111 ± 0.306
1.481PheGln: 1.481 ± 0.353
1.389PheArg: 1.389 ± 0.39
2.315PheSer: 2.315 ± 0.508
1.944PheThr: 1.944 ± 0.516
1.574PheVal: 1.574 ± 0.434
0.37PheTrp: 0.37 ± 0.172
1.389PheTyr: 1.389 ± 0.369
0.0PheXaa: 0.0 ± 0.0
Gly
3.426GlyAla: 3.426 ± 0.593
0.185GlyCys: 0.185 ± 0.132
2.315GlyAsp: 2.315 ± 0.379
2.129GlyGlu: 2.129 ± 0.358
3.148GlyPhe: 3.148 ± 0.556
3.24GlyGly: 3.24 ± 0.626
0.463GlyHis: 0.463 ± 0.178
4.537GlyIle: 4.537 ± 0.829
6.481GlyLys: 6.481 ± 0.759
4.907GlyLeu: 4.907 ± 0.725
2.037GlyMet: 2.037 ± 0.546
4.444GlyAsn: 4.444 ± 0.842
0.278GlyPro: 0.278 ± 0.144
3.333GlyGln: 3.333 ± 0.483
2.592GlyArg: 2.592 ± 0.651
2.592GlySer: 2.592 ± 0.496
5.0GlyThr: 5.0 ± 0.785
3.889GlyVal: 3.889 ± 0.543
0.741GlyTrp: 0.741 ± 0.278
2.222GlyTyr: 2.222 ± 0.476
0.0GlyXaa: 0.0 ± 0.0
His
1.111HisAla: 1.111 ± 0.378
0.093HisCys: 0.093 ± 0.095
0.648HisAsp: 0.648 ± 0.225
0.648HisGlu: 0.648 ± 0.206
0.463HisPhe: 0.463 ± 0.244
1.481HisGly: 1.481 ± 0.292
0.37HisHis: 0.37 ± 0.186
0.926HisIle: 0.926 ± 0.298
2.037HisLys: 2.037 ± 0.421
1.018HisLeu: 1.018 ± 0.302
0.463HisMet: 0.463 ± 0.189
1.296HisAsn: 1.296 ± 0.293
0.37HisPro: 0.37 ± 0.172
0.556HisGln: 0.556 ± 0.222
0.648HisArg: 0.648 ± 0.238
1.018HisSer: 1.018 ± 0.282
0.926HisThr: 0.926 ± 0.259
0.741HisVal: 0.741 ± 0.308
0.37HisTrp: 0.37 ± 0.153
0.556HisTyr: 0.556 ± 0.204
0.0HisXaa: 0.0 ± 0.0
Ile
4.259IleAla: 4.259 ± 0.527
0.556IleCys: 0.556 ± 0.186
5.555IleAsp: 5.555 ± 0.722
3.611IleGlu: 3.611 ± 0.719
2.129IlePhe: 2.129 ± 0.403
4.074IleGly: 4.074 ± 0.98
1.296IleHis: 1.296 ± 0.345
2.592IleIle: 2.592 ± 0.534
7.314IleLys: 7.314 ± 0.659
3.426IleLeu: 3.426 ± 0.522
1.296IleMet: 1.296 ± 0.27
4.259IleAsn: 4.259 ± 0.7
2.407IlePro: 2.407 ± 0.465
2.5IleGln: 2.5 ± 0.435
3.055IleArg: 3.055 ± 0.453
4.166IleSer: 4.166 ± 0.57
3.518IleThr: 3.518 ± 0.59
3.703IleVal: 3.703 ± 0.713
0.833IleTrp: 0.833 ± 0.279
2.5IleTyr: 2.5 ± 0.409
0.0IleXaa: 0.0 ± 0.0
Lys
7.129LysAla: 7.129 ± 0.99
0.741LysCys: 0.741 ± 0.265
4.907LysAsp: 4.907 ± 0.726
6.388LysGlu: 6.388 ± 0.865
2.407LysPhe: 2.407 ± 0.403
3.889LysGly: 3.889 ± 0.536
2.129LysHis: 2.129 ± 0.517
6.481LysIle: 6.481 ± 0.707
8.147LysLys: 8.147 ± 1.445
6.759LysLeu: 6.759 ± 0.564
2.315LysMet: 2.315 ± 0.521
6.573LysAsn: 6.573 ± 0.754
4.444LysPro: 4.444 ± 0.565
3.148LysGln: 3.148 ± 0.542
4.074LysArg: 4.074 ± 0.743
5.185LysSer: 5.185 ± 0.843
5.833LysThr: 5.833 ± 0.945
6.851LysVal: 6.851 ± 0.918
1.018LysTrp: 1.018 ± 0.25
4.814LysTyr: 4.814 ± 0.763
0.0LysXaa: 0.0 ± 0.0
Leu
6.573LeuAla: 6.573 ± 0.855
0.278LeuCys: 0.278 ± 0.146
5.37LeuAsp: 5.37 ± 0.747
4.722LeuGlu: 4.722 ± 0.581
2.685LeuPhe: 2.685 ± 0.546
4.814LeuGly: 4.814 ± 0.703
1.111LeuHis: 1.111 ± 0.36
4.814LeuIle: 4.814 ± 0.582
8.055LeuLys: 8.055 ± 0.957
5.555LeuLeu: 5.555 ± 0.676
2.685LeuMet: 2.685 ± 0.414
5.555LeuAsn: 5.555 ± 0.88
3.055LeuPro: 3.055 ± 0.609
2.685LeuGln: 2.685 ± 0.481
2.592LeuArg: 2.592 ± 0.501
5.092LeuSer: 5.092 ± 0.588
4.351LeuThr: 4.351 ± 0.802
4.722LeuVal: 4.722 ± 0.433
0.741LeuTrp: 0.741 ± 0.228
3.518LeuTyr: 3.518 ± 0.552
0.0LeuXaa: 0.0 ± 0.0
Met
1.481MetAla: 1.481 ± 0.379
0.093MetCys: 0.093 ± 0.086
2.037MetAsp: 2.037 ± 0.402
1.759MetGlu: 1.759 ± 0.432
1.296MetPhe: 1.296 ± 0.316
0.741MetGly: 0.741 ± 0.237
0.37MetHis: 0.37 ± 0.172
1.944MetIle: 1.944 ± 0.403
2.407MetLys: 2.407 ± 0.461
1.667MetLeu: 1.667 ± 0.396
0.463MetMet: 0.463 ± 0.21
1.759MetAsn: 1.759 ± 0.399
1.759MetPro: 1.759 ± 0.455
1.481MetGln: 1.481 ± 0.331
0.556MetArg: 0.556 ± 0.198
2.037MetSer: 2.037 ± 0.375
2.222MetThr: 2.222 ± 0.346
1.389MetVal: 1.389 ± 0.323
0.463MetTrp: 0.463 ± 0.229
0.556MetTyr: 0.556 ± 0.204
0.0MetXaa: 0.0 ± 0.0
Asn
5.462AsnAla: 5.462 ± 0.792
0.556AsnCys: 0.556 ± 0.217
4.259AsnAsp: 4.259 ± 0.81
3.981AsnGlu: 3.981 ± 0.602
2.315AsnPhe: 2.315 ± 0.523
4.537AsnGly: 4.537 ± 0.513
1.759AsnHis: 1.759 ± 0.35
3.426AsnIle: 3.426 ± 0.501
4.074AsnLys: 4.074 ± 0.625
5.37AsnLeu: 5.37 ± 0.621
1.944AsnMet: 1.944 ± 0.508
4.722AsnAsn: 4.722 ± 0.891
2.685AsnPro: 2.685 ± 0.493
2.315AsnGln: 2.315 ± 0.575
2.778AsnArg: 2.778 ± 0.535
5.555AsnSer: 5.555 ± 0.881
3.333AsnThr: 3.333 ± 0.581
4.166AsnVal: 4.166 ± 0.543
0.278AsnTrp: 0.278 ± 0.138
3.426AsnTyr: 3.426 ± 0.558
0.0AsnXaa: 0.0 ± 0.0
Pro
2.315ProAla: 2.315 ± 0.487
0.37ProCys: 0.37 ± 0.181
2.407ProAsp: 2.407 ± 0.606
2.407ProGlu: 2.407 ± 0.409
1.111ProPhe: 1.111 ± 0.299
1.296ProGly: 1.296 ± 0.283
0.278ProHis: 0.278 ± 0.175
2.315ProIle: 2.315 ± 0.383
3.981ProLys: 3.981 ± 0.893
2.5ProLeu: 2.5 ± 0.441
0.093ProMet: 0.093 ± 0.089
2.592ProAsn: 2.592 ± 0.444
0.833ProPro: 0.833 ± 0.229
0.926ProGln: 0.926 ± 0.367
0.556ProArg: 0.556 ± 0.195
2.963ProSer: 2.963 ± 0.493
1.852ProThr: 1.852 ± 0.49
2.315ProVal: 2.315 ± 0.48
0.37ProTrp: 0.37 ± 0.206
1.111ProTyr: 1.111 ± 0.306
0.0ProXaa: 0.0 ± 0.0
Gln
3.426GlnAla: 3.426 ± 0.51
0.278GlnCys: 0.278 ± 0.149
2.407GlnAsp: 2.407 ± 0.519
1.759GlnGlu: 1.759 ± 0.489
1.667GlnPhe: 1.667 ± 0.327
2.5GlnGly: 2.5 ± 0.47
0.463GlnHis: 0.463 ± 0.212
3.703GlnIle: 3.703 ± 0.48
4.629GlnLys: 4.629 ± 0.815
3.703GlnLeu: 3.703 ± 0.651
1.574GlnMet: 1.574 ± 0.448
2.5GlnAsn: 2.5 ± 0.561
1.018GlnPro: 1.018 ± 0.325
2.129GlnGln: 2.129 ± 0.386
1.018GlnArg: 1.018 ± 0.22
2.685GlnSer: 2.685 ± 0.468
2.222GlnThr: 2.222 ± 0.491
2.963GlnVal: 2.963 ± 0.506
0.185GlnTrp: 0.185 ± 0.111
1.852GlnTyr: 1.852 ± 0.345
0.0GlnXaa: 0.0 ± 0.0
Arg
3.24ArgAla: 3.24 ± 0.603
0.278ArgCys: 0.278 ± 0.166
1.667ArgAsp: 1.667 ± 0.374
2.592ArgGlu: 2.592 ± 0.525
1.389ArgPhe: 1.389 ± 0.286
2.037ArgGly: 2.037 ± 0.51
0.648ArgHis: 0.648 ± 0.226
2.963ArgIle: 2.963 ± 0.579
2.778ArgLys: 2.778 ± 0.57
3.518ArgLeu: 3.518 ± 0.69
1.018ArgMet: 1.018 ± 0.345
2.037ArgAsn: 2.037 ± 0.45
1.018ArgPro: 1.018 ± 0.354
1.204ArgGln: 1.204 ± 0.368
1.574ArgArg: 1.574 ± 0.401
2.222ArgSer: 2.222 ± 0.48
2.5ArgThr: 2.5 ± 0.419
2.315ArgVal: 2.315 ± 0.412
0.648ArgTrp: 0.648 ± 0.24
1.574ArgTyr: 1.574 ± 0.399
0.0ArgXaa: 0.0 ± 0.0
Ser
4.907SerAla: 4.907 ± 1.023
0.185SerCys: 0.185 ± 0.136
3.889SerAsp: 3.889 ± 0.514
3.611SerGlu: 3.611 ± 0.585
2.222SerPhe: 2.222 ± 0.517
4.166SerGly: 4.166 ± 0.705
0.741SerHis: 0.741 ± 0.228
3.981SerIle: 3.981 ± 0.536
5.555SerLys: 5.555 ± 0.7
5.185SerLeu: 5.185 ± 0.698
2.129SerMet: 2.129 ± 0.487
4.722SerAsn: 4.722 ± 0.818
1.296SerPro: 1.296 ± 0.272
2.87SerGln: 2.87 ± 0.706
2.5SerArg: 2.5 ± 0.476
5.0SerSer: 5.0 ± 1.133
3.703SerThr: 3.703 ± 0.567
3.889SerVal: 3.889 ± 0.67
0.648SerTrp: 0.648 ± 0.237
3.055SerTyr: 3.055 ± 0.62
0.0SerXaa: 0.0 ± 0.0
Thr
4.259ThrAla: 4.259 ± 0.768
0.648ThrCys: 0.648 ± 0.297
3.889ThrAsp: 3.889 ± 0.6
2.315ThrGlu: 2.315 ± 0.515
1.481ThrPhe: 1.481 ± 0.397
4.537ThrGly: 4.537 ± 0.729
1.204ThrHis: 1.204 ± 0.431
4.722ThrIle: 4.722 ± 0.827
5.925ThrLys: 5.925 ± 0.731
4.537ThrLeu: 4.537 ± 0.609
1.111ThrMet: 1.111 ± 0.345
2.963ThrAsn: 2.963 ± 0.457
2.129ThrPro: 2.129 ± 0.412
2.5ThrGln: 2.5 ± 0.689
2.592ThrArg: 2.592 ± 0.484
3.611ThrSer: 3.611 ± 0.571
3.703ThrThr: 3.703 ± 0.538
4.814ThrVal: 4.814 ± 0.72
0.741ThrTrp: 0.741 ± 0.31
2.315ThrTyr: 2.315 ± 0.475
0.0ThrXaa: 0.0 ± 0.0
Val
5.0ValAla: 5.0 ± 0.749
0.556ValCys: 0.556 ± 0.213
5.462ValAsp: 5.462 ± 0.652
3.426ValGlu: 3.426 ± 0.649
1.944ValPhe: 1.944 ± 0.337
3.889ValGly: 3.889 ± 0.755
1.018ValHis: 1.018 ± 0.306
3.611ValIle: 3.611 ± 0.44
5.925ValLys: 5.925 ± 0.868
5.925ValLeu: 5.925 ± 0.733
0.741ValMet: 0.741 ± 0.227
5.0ValAsn: 5.0 ± 0.722
2.778ValPro: 2.778 ± 0.458
3.055ValGln: 3.055 ± 0.535
1.852ValArg: 1.852 ± 0.403
4.351ValSer: 4.351 ± 0.665
3.796ValThr: 3.796 ± 0.711
4.537ValVal: 4.537 ± 0.813
0.741ValTrp: 0.741 ± 0.268
2.592ValTyr: 2.592 ± 0.623
0.0ValXaa: 0.0 ± 0.0
Trp
0.463TrpAla: 0.463 ± 0.228
0.37TrpCys: 0.37 ± 0.244
0.926TrpAsp: 0.926 ± 0.309
0.648TrpGlu: 0.648 ± 0.274
0.741TrpPhe: 0.741 ± 0.266
0.556TrpGly: 0.556 ± 0.209
0.278TrpHis: 0.278 ± 0.239
0.278TrpIle: 0.278 ± 0.165
1.481TrpLys: 1.481 ± 0.45
1.204TrpLeu: 1.204 ± 0.33
0.093TrpMet: 0.093 ± 0.089
0.556TrpAsn: 0.556 ± 0.201
0.093TrpPro: 0.093 ± 0.093
0.833TrpGln: 0.833 ± 0.265
0.463TrpArg: 0.463 ± 0.195
0.556TrpSer: 0.556 ± 0.231
0.741TrpThr: 0.741 ± 0.244
1.111TrpVal: 1.111 ± 0.292
0.0TrpTrp: 0.0 ± 0.0
0.185TrpTyr: 0.185 ± 0.135
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.5TyrAla: 2.5 ± 0.489
0.463TyrCys: 0.463 ± 0.227
2.5TyrAsp: 2.5 ± 0.631
3.703TyrGlu: 3.703 ± 0.699
1.481TyrPhe: 1.481 ± 0.454
3.055TyrGly: 3.055 ± 0.608
0.463TyrHis: 0.463 ± 0.195
2.129TyrIle: 2.129 ± 0.447
2.963TyrLys: 2.963 ± 0.649
3.611TyrLeu: 3.611 ± 0.571
1.389TyrMet: 1.389 ± 0.45
1.852TyrAsn: 1.852 ± 0.419
0.926TyrPro: 0.926 ± 0.258
2.5TyrGln: 2.5 ± 0.649
1.667TyrArg: 1.667 ± 0.405
2.778TyrSer: 2.778 ± 0.451
2.87TyrThr: 2.87 ± 0.496
3.333TyrVal: 3.333 ± 0.554
0.648TyrTrp: 0.648 ± 0.217
1.204TyrTyr: 1.204 ± 0.298
0.093TyrXaa: 0.093 ± 0.084
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.093XaaIle: 0.093 ± 0.084
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.093XaaVal: 0.093 ± 0.095
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (10802 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski