Mesitornis unicolor (brown roatelo)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9395 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A091S499|A0A091S499_9GRUI HECT-type E3 ubiquitin transferase (Fragment) OS=Mesitornis unicolor OX=54374 GN=N332_14637 PE=4 SV=1
VV1 pKa = 7.41PDD3 pKa = 3.61SEE5 pKa = 4.31EE6 pKa = 3.98DD7 pKa = 3.09KK8 pKa = 11.24GYY10 pKa = 11.04NIYY13 pKa = 11.04DD14 pKa = 3.79DD15 pKa = 4.95VYY17 pKa = 10.39EE18 pKa = 5.05DD19 pKa = 3.81PASASSITPVPEE31 pKa = 4.77SMDD34 pKa = 3.79SNQDD38 pKa = 3.3YY39 pKa = 11.48SEE41 pKa = 5.02DD42 pKa = 3.52SHH44 pKa = 8.25SNSVASQHH52 pKa = 6.48AADD55 pKa = 4.94GEE57 pKa = 4.62DD58 pKa = 3.61VSSSSHH64 pKa = 6.21HH65 pKa = 6.15GADD68 pKa = 4.33GEE70 pKa = 4.42DD71 pKa = 3.28DD72 pKa = 3.92HH73 pKa = 7.38PYY75 pKa = 10.8QEE77 pKa = 4.24MSLMSRR83 pKa = 11.84TVVDD87 pKa = 3.28KK88 pKa = 9.99HH89 pKa = 5.97TEE91 pKa = 3.87DD92 pKa = 4.75VMFEE96 pKa = 4.49SNTDD100 pKa = 3.9FPPYY104 pKa = 10.29LQLQQSQSTSSNSSTSLKK122 pKa = 10.59FPFNNNCRR130 pKa = 11.84EE131 pKa = 3.94TDD133 pKa = 3.58NNQDD137 pKa = 2.99NYY139 pKa = 10.7EE140 pKa = 3.54IFMFVKK146 pKa = 10.68

Molecular weight: 16.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A091R1H9|A0A091R1H9_9GRUI F-box only protein 31 (Fragment) OS=Mesitornis unicolor OX=54374 GN=N332_12591 PE=3 SV=1
LL1 pKa = 6.71GRR3 pKa = 11.84SVLGRR8 pKa = 11.84SVLGRR13 pKa = 11.84SVLGRR18 pKa = 11.84SVLGRR23 pKa = 11.84SVLGRR28 pKa = 11.84SVLGRR33 pKa = 11.84SVLGRR38 pKa = 11.84SVLGRR43 pKa = 11.84SVLGRR48 pKa = 11.84SVLGRR53 pKa = 11.84SVLGRR58 pKa = 11.84SVLGG62 pKa = 3.7

Molecular weight: 6.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9395

0

9395

3786929

33

5556

403.1

45.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.445 ± 0.021

2.241 ± 0.019

5.009 ± 0.019

7.158 ± 0.034

3.938 ± 0.018

5.874 ± 0.037

2.552 ± 0.014

4.975 ± 0.02

6.408 ± 0.03

9.757 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.254 ± 0.01

4.12 ± 0.017

5.285 ± 0.038

4.682 ± 0.026

5.202 ± 0.019

8.191 ± 0.035

5.385 ± 0.018

6.328 ± 0.021

1.186 ± 0.008

3.005 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski