Amino acid dipepetide frequency for Micrococcales bacterium KH10

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.206AlaAla: 17.206 ± 0.22
0.665AlaCys: 0.665 ± 0.033
7.542AlaAsp: 7.542 ± 0.102
6.821AlaGlu: 6.821 ± 0.103
2.995AlaPhe: 2.995 ± 0.07
10.267AlaGly: 10.267 ± 0.114
2.381AlaHis: 2.381 ± 0.057
5.587AlaIle: 5.587 ± 0.095
3.732AlaLys: 3.732 ± 0.077
11.771AlaLeu: 11.771 ± 0.169
2.451AlaMet: 2.451 ± 0.058
3.017AlaAsn: 3.017 ± 0.062
5.316AlaPro: 5.316 ± 0.104
4.772AlaGln: 4.772 ± 0.089
7.692AlaArg: 7.692 ± 0.132
6.577AlaSer: 6.577 ± 0.091
8.413AlaThr: 8.413 ± 0.13
10.337AlaVal: 10.337 ± 0.127
1.518AlaTrp: 1.518 ± 0.047
2.241AlaTyr: 2.241 ± 0.06
0.0AlaXaa: 0.0 ± 0.0
Cys
0.677CysAla: 0.677 ± 0.029
0.045CysCys: 0.045 ± 0.008
0.386CysAsp: 0.386 ± 0.022
0.3CysGlu: 0.3 ± 0.019
0.133CysPhe: 0.133 ± 0.013
0.616CysGly: 0.616 ± 0.029
0.123CysHis: 0.123 ± 0.012
0.181CysIle: 0.181 ± 0.014
0.104CysLys: 0.104 ± 0.011
0.479CysLeu: 0.479 ± 0.025
0.08CysMet: 0.08 ± 0.011
0.181CysAsn: 0.181 ± 0.016
0.317CysPro: 0.317 ± 0.02
0.205CysGln: 0.205 ± 0.017
0.366CysArg: 0.366 ± 0.022
0.388CysSer: 0.388 ± 0.025
0.387CysThr: 0.387 ± 0.025
0.462CysVal: 0.462 ± 0.025
0.076CysTrp: 0.076 ± 0.009
0.143CysTyr: 0.143 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
6.902AspAla: 6.902 ± 0.087
0.288AspCys: 0.288 ± 0.02
4.374AspAsp: 4.374 ± 0.104
5.208AspGlu: 5.208 ± 0.086
1.785AspPhe: 1.785 ± 0.044
5.792AspGly: 5.792 ± 0.116
1.239AspHis: 1.239 ± 0.038
2.768AspIle: 2.768 ± 0.056
1.421AspLys: 1.421 ± 0.04
6.215AspLeu: 6.215 ± 0.099
0.94AspMet: 0.94 ± 0.03
1.548AspAsn: 1.548 ± 0.053
4.117AspPro: 4.117 ± 0.093
2.197AspGln: 2.197 ± 0.048
3.839AspArg: 3.839 ± 0.083
3.374AspSer: 3.374 ± 0.068
3.31AspThr: 3.31 ± 0.072
5.289AspVal: 5.289 ± 0.083
0.847AspTrp: 0.847 ± 0.033
1.431AspTyr: 1.431 ± 0.051
0.0AspXaa: 0.0 ± 0.0
Glu
6.085GluAla: 6.085 ± 0.102
0.292GluCys: 0.292 ± 0.019
2.763GluAsp: 2.763 ± 0.063
2.719GluGlu: 2.719 ± 0.074
1.806GluPhe: 1.806 ± 0.05
3.487GluGly: 3.487 ± 0.065
1.497GluHis: 1.497 ± 0.044
3.118GluIle: 3.118 ± 0.062
1.389GluLys: 1.389 ± 0.046
6.547GluLeu: 6.547 ± 0.11
0.984GluMet: 0.984 ± 0.037
1.461GluAsn: 1.461 ± 0.048
3.049GluPro: 3.049 ± 0.066
2.664GluGln: 2.664 ± 0.065
4.294GluArg: 4.294 ± 0.084
3.215GluSer: 3.215 ± 0.064
3.095GluThr: 3.095 ± 0.066
4.796GluVal: 4.796 ± 0.078
0.674GluTrp: 0.674 ± 0.028
1.213GluTyr: 1.213 ± 0.04
0.0GluXaa: 0.0 ± 0.0
Phe
3.697PheAla: 3.697 ± 0.072
0.175PheCys: 0.175 ± 0.014
2.246PheAsp: 2.246 ± 0.06
1.728PheGlu: 1.728 ± 0.051
0.927PhePhe: 0.927 ± 0.038
2.835PheGly: 2.835 ± 0.058
0.56PheHis: 0.56 ± 0.027
1.175PheIle: 1.175 ± 0.038
0.704PheLys: 0.704 ± 0.033
2.248PheLeu: 2.248 ± 0.058
0.384PheMet: 0.384 ± 0.022
0.842PheAsn: 0.842 ± 0.036
1.278PhePro: 1.278 ± 0.041
0.751PheGln: 0.751 ± 0.031
1.461PheArg: 1.461 ± 0.045
1.805PheSer: 1.805 ± 0.052
2.14PheThr: 2.14 ± 0.057
2.372PheVal: 2.372 ± 0.058
0.377PheTrp: 0.377 ± 0.021
0.605PheTyr: 0.605 ± 0.024
0.0PheXaa: 0.0 ± 0.0
Gly
8.877GlyAla: 8.877 ± 0.129
0.553GlyCys: 0.553 ± 0.028
5.066GlyAsp: 5.066 ± 0.095
4.281GlyGlu: 4.281 ± 0.08
2.737GlyPhe: 2.737 ± 0.062
6.949GlyGly: 6.949 ± 0.133
1.788GlyHis: 1.788 ± 0.048
4.221GlyIle: 4.221 ± 0.08
3.211GlyLys: 3.211 ± 0.074
7.553GlyLeu: 7.553 ± 0.113
1.799GlyMet: 1.799 ± 0.047
2.435GlyAsn: 2.435 ± 0.091
3.54GlyPro: 3.54 ± 0.079
3.209GlyGln: 3.209 ± 0.079
5.394GlyArg: 5.394 ± 0.088
5.683GlySer: 5.683 ± 0.096
6.13GlyThr: 6.13 ± 0.135
6.62GlyVal: 6.62 ± 0.094
1.431GlyTrp: 1.431 ± 0.044
2.251GlyTyr: 2.251 ± 0.062
0.0GlyXaa: 0.0 ± 0.0
His
2.166HisAla: 2.166 ± 0.056
0.163HisCys: 0.163 ± 0.016
1.389HisAsp: 1.389 ± 0.047
1.156HisGlu: 1.156 ± 0.037
0.555HisPhe: 0.555 ± 0.027
1.921HisGly: 1.921 ± 0.046
0.597HisHis: 0.597 ± 0.03
0.963HisIle: 0.963 ± 0.034
0.392HisLys: 0.392 ± 0.021
1.865HisLeu: 1.865 ± 0.055
0.311HisMet: 0.311 ± 0.019
0.521HisAsn: 0.521 ± 0.022
1.28HisPro: 1.28 ± 0.049
0.684HisGln: 0.684 ± 0.03
1.624HisArg: 1.624 ± 0.047
1.139HisSer: 1.139 ± 0.04
1.159HisThr: 1.159 ± 0.039
1.587HisVal: 1.587 ± 0.045
0.346HisTrp: 0.346 ± 0.021
0.51HisTyr: 0.51 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
6.927IleAla: 6.927 ± 0.089
0.301IleCys: 0.301 ± 0.02
3.914IleAsp: 3.914 ± 0.074
3.223IleGlu: 3.223 ± 0.063
1.234IlePhe: 1.234 ± 0.042
4.301IleGly: 4.301 ± 0.087
0.77IleHis: 0.77 ± 0.03
1.942IleIle: 1.942 ± 0.056
1.426IleLys: 1.426 ± 0.043
3.373IleLeu: 3.373 ± 0.077
0.708IleMet: 0.708 ± 0.03
1.313IleAsn: 1.313 ± 0.047
2.385IlePro: 2.385 ± 0.051
1.112IleGln: 1.112 ± 0.041
2.912IleArg: 2.912 ± 0.056
3.055IleSer: 3.055 ± 0.057
3.426IleThr: 3.426 ± 0.089
4.54IleVal: 4.54 ± 0.079
0.564IleTrp: 0.564 ± 0.026
0.895IleTyr: 0.895 ± 0.035
0.0IleXaa: 0.0 ± 0.0
Lys
3.348LysAla: 3.348 ± 0.089
0.132LysCys: 0.132 ± 0.015
1.392LysAsp: 1.392 ± 0.041
1.205LysGlu: 1.205 ± 0.056
0.767LysPhe: 0.767 ± 0.036
1.806LysGly: 1.806 ± 0.056
0.512LysHis: 0.512 ± 0.024
1.538LysIle: 1.538 ± 0.057
1.711LysLys: 1.711 ± 0.081
2.428LysLeu: 2.428 ± 0.071
0.493LysMet: 0.493 ± 0.024
0.888LysAsn: 0.888 ± 0.038
1.551LysPro: 1.551 ± 0.053
1.012LysGln: 1.012 ± 0.037
1.95LysArg: 1.95 ± 0.051
1.718LysSer: 1.718 ± 0.048
1.973LysThr: 1.973 ± 0.055
2.75LysVal: 2.75 ± 0.08
0.411LysTrp: 0.411 ± 0.023
0.686LysTyr: 0.686 ± 0.025
0.0LysXaa: 0.0 ± 0.0
Leu
12.213LeuAla: 12.213 ± 0.19
0.528LeuCys: 0.528 ± 0.026
6.268LeuAsp: 6.268 ± 0.11
4.703LeuGlu: 4.703 ± 0.091
2.17LeuPhe: 2.17 ± 0.066
7.714LeuGly: 7.714 ± 0.1
1.813LeuHis: 1.813 ± 0.048
4.426LeuIle: 4.426 ± 0.079
2.158LeuLys: 2.158 ± 0.061
8.379LeuLeu: 8.379 ± 0.151
1.463LeuMet: 1.463 ± 0.046
2.244LeuAsn: 2.244 ± 0.047
5.058LeuPro: 5.058 ± 0.082
2.674LeuGln: 2.674 ± 0.061
6.977LeuArg: 6.977 ± 0.113
5.854LeuSer: 5.854 ± 0.086
6.669LeuThr: 6.669 ± 0.093
8.424LeuVal: 8.424 ± 0.123
1.14LeuTrp: 1.14 ± 0.039
1.582LeuTyr: 1.582 ± 0.049
0.0LeuXaa: 0.0 ± 0.0
Met
2.161MetAla: 2.161 ± 0.051
0.101MetCys: 0.101 ± 0.01
0.872MetAsp: 0.872 ± 0.032
0.667MetGlu: 0.667 ± 0.029
0.471MetPhe: 0.471 ± 0.026
1.239MetGly: 1.239 ± 0.038
0.306MetHis: 0.306 ± 0.019
0.945MetIle: 0.945 ± 0.033
0.512MetLys: 0.512 ± 0.027
1.723MetLeu: 1.723 ± 0.046
0.36MetMet: 0.36 ± 0.021
0.568MetAsn: 0.568 ± 0.025
0.985MetPro: 0.985 ± 0.033
0.541MetGln: 0.541 ± 0.026
1.344MetArg: 1.344 ± 0.041
1.606MetSer: 1.606 ± 0.038
1.756MetThr: 1.756 ± 0.048
1.363MetVal: 1.363 ± 0.04
0.26MetTrp: 0.26 ± 0.019
0.306MetTyr: 0.306 ± 0.019
0.0MetXaa: 0.0 ± 0.0
Asn
3.012AsnAla: 3.012 ± 0.059
0.176AsnCys: 0.176 ± 0.016
1.68AsnAsp: 1.68 ± 0.053
1.402AsnGlu: 1.402 ± 0.047
0.788AsnPhe: 0.788 ± 0.033
2.582AsnGly: 2.582 ± 0.098
0.465AsnHis: 0.465 ± 0.025
1.132AsnIle: 1.132 ± 0.037
0.777AsnLys: 0.777 ± 0.039
2.366AsnLeu: 2.366 ± 0.061
0.491AsnMet: 0.491 ± 0.026
0.925AsnAsn: 0.925 ± 0.041
1.983AsnPro: 1.983 ± 0.067
0.947AsnGln: 0.947 ± 0.032
1.551AsnArg: 1.551 ± 0.042
1.556AsnSer: 1.556 ± 0.05
1.678AsnThr: 1.678 ± 0.055
2.244AsnVal: 2.244 ± 0.066
0.433AsnTrp: 0.433 ± 0.027
0.728AsnTyr: 0.728 ± 0.035
0.0AsnXaa: 0.0 ± 0.0
Pro
6.407ProAla: 6.407 ± 0.118
0.214ProCys: 0.214 ± 0.015
3.745ProAsp: 3.745 ± 0.076
3.284ProGlu: 3.284 ± 0.063
1.334ProPhe: 1.334 ± 0.046
4.933ProGly: 4.933 ± 0.111
1.12ProHis: 1.12 ± 0.037
2.145ProIle: 2.145 ± 0.05
1.306ProLys: 1.306 ± 0.05
4.094ProLeu: 4.094 ± 0.087
0.864ProMet: 0.864 ± 0.032
1.407ProAsn: 1.407 ± 0.051
2.174ProPro: 2.174 ± 0.069
1.998ProGln: 1.998 ± 0.052
2.988ProArg: 2.988 ± 0.06
3.271ProSer: 3.271 ± 0.064
3.62ProThr: 3.62 ± 0.082
4.717ProVal: 4.717 ± 0.081
0.804ProTrp: 0.804 ± 0.033
1.072ProTyr: 1.072 ± 0.04
0.0ProXaa: 0.0 ± 0.0
Gln
4.09GlnAla: 4.09 ± 0.09
0.217GlnCys: 0.217 ± 0.017
1.717GlnAsp: 1.717 ± 0.047
1.55GlnGlu: 1.55 ± 0.04
1.108GlnPhe: 1.108 ± 0.037
2.518GlnGly: 2.518 ± 0.058
0.796GlnHis: 0.796 ± 0.029
2.065GlnIle: 2.065 ± 0.059
0.717GlnLys: 0.717 ± 0.034
3.435GlnLeu: 3.435 ± 0.068
0.701GlnMet: 0.701 ± 0.026
0.858GlnAsn: 0.858 ± 0.037
1.967GlnPro: 1.967 ± 0.062
1.679GlnGln: 1.679 ± 0.057
2.945GlnArg: 2.945 ± 0.071
2.096GlnSer: 2.096 ± 0.047
2.026GlnThr: 2.026 ± 0.054
3.391GlnVal: 3.391 ± 0.067
0.745GlnTrp: 0.745 ± 0.032
0.886GlnTyr: 0.886 ± 0.03
0.0GlnXaa: 0.0 ± 0.0
Arg
7.324ArgAla: 7.324 ± 0.113
0.368ArgCys: 0.368 ± 0.021
4.053ArgAsp: 4.053 ± 0.074
3.782ArgGlu: 3.782 ± 0.084
2.057ArgPhe: 2.057 ± 0.052
4.895ArgGly: 4.895 ± 0.086
1.543ArgHis: 1.543 ± 0.047
3.602ArgIle: 3.602 ± 0.066
1.91ArgLys: 1.91 ± 0.05
6.366ArgLeu: 6.366 ± 0.121
1.47ArgMet: 1.47 ± 0.04
1.79ArgAsn: 1.79 ± 0.053
3.206ArgPro: 3.206 ± 0.072
2.526ArgGln: 2.526 ± 0.078
5.619ArgArg: 5.619 ± 0.131
4.224ArgSer: 4.224 ± 0.073
4.344ArgThr: 4.344 ± 0.086
5.382ArgVal: 5.382 ± 0.074
1.236ArgTrp: 1.236 ± 0.047
1.666ArgTyr: 1.666 ± 0.044
0.0ArgXaa: 0.0 ± 0.0
Ser
7.319SerAla: 7.319 ± 0.089
0.342SerCys: 0.342 ± 0.023
4.047SerAsp: 4.047 ± 0.076
3.072SerGlu: 3.072 ± 0.068
1.793SerPhe: 1.793 ± 0.047
6.18SerGly: 6.18 ± 0.106
1.306SerHis: 1.306 ± 0.038
2.712SerIle: 2.712 ± 0.06
1.606SerLys: 1.606 ± 0.051
5.347SerLeu: 5.347 ± 0.069
1.259SerMet: 1.259 ± 0.042
1.861SerAsn: 1.861 ± 0.056
3.022SerPro: 3.022 ± 0.057
2.159SerGln: 2.159 ± 0.054
3.889SerArg: 3.889 ± 0.077
4.473SerSer: 4.473 ± 0.091
4.134SerThr: 4.134 ± 0.082
5.084SerVal: 5.084 ± 0.079
1.019SerTrp: 1.019 ± 0.033
1.42SerTyr: 1.42 ± 0.051
0.0SerXaa: 0.0 ± 0.0
Thr
8.367ThrAla: 8.367 ± 0.128
0.34ThrCys: 0.34 ± 0.02
3.939ThrAsp: 3.939 ± 0.079
3.415ThrGlu: 3.415 ± 0.07
2.016ThrPhe: 2.016 ± 0.055
6.226ThrGly: 6.226 ± 0.113
1.201ThrHis: 1.201 ± 0.035
3.557ThrIle: 3.557 ± 0.077
1.88ThrLys: 1.88 ± 0.061
5.951ThrLeu: 5.951 ± 0.088
1.252ThrMet: 1.252 ± 0.04
1.765ThrAsn: 1.765 ± 0.058
3.98ThrPro: 3.98 ± 0.094
2.169ThrGln: 2.169 ± 0.054
3.838ThrArg: 3.838 ± 0.076
4.475ThrSer: 4.475 ± 0.086
4.898ThrThr: 4.898 ± 0.101
6.894ThrVal: 6.894 ± 0.145
1.039ThrTrp: 1.039 ± 0.045
1.617ThrTyr: 1.617 ± 0.06
0.0ThrXaa: 0.0 ± 0.0
Val
10.848ValAla: 10.848 ± 0.117
0.492ValCys: 0.492 ± 0.022
5.229ValAsp: 5.229 ± 0.086
4.71ValGlu: 4.71 ± 0.086
2.389ValPhe: 2.389 ± 0.055
6.532ValGly: 6.532 ± 0.091
1.558ValHis: 1.558 ± 0.045
4.727ValIle: 4.727 ± 0.077
2.508ValLys: 2.508 ± 0.079
8.482ValLeu: 8.482 ± 0.147
1.504ValMet: 1.504 ± 0.044
2.19ValAsn: 2.19 ± 0.066
4.528ValPro: 4.528 ± 0.08
2.559ValGln: 2.559 ± 0.051
5.673ValArg: 5.673 ± 0.099
5.229ValSer: 5.229 ± 0.076
7.407ValThr: 7.407 ± 0.148
8.023ValVal: 8.023 ± 0.126
1.07ValTrp: 1.07 ± 0.036
1.452ValTyr: 1.452 ± 0.044
0.0ValXaa: 0.0 ± 0.0
Trp
1.423TrpAla: 1.423 ± 0.044
0.127TrpCys: 0.127 ± 0.013
0.825TrpAsp: 0.825 ± 0.033
0.628TrpGlu: 0.628 ± 0.031
0.528TrpPhe: 0.528 ± 0.033
1.049TrpGly: 1.049 ± 0.038
0.362TrpHis: 0.362 ± 0.02
0.726TrpIle: 0.726 ± 0.032
0.316TrpLys: 0.316 ± 0.021
1.622TrpLeu: 1.622 ± 0.051
0.294TrpMet: 0.294 ± 0.021
0.511TrpAsn: 0.511 ± 0.026
0.633TrpPro: 0.633 ± 0.031
0.761TrpGln: 0.761 ± 0.031
1.201TrpArg: 1.201 ± 0.048
1.015TrpSer: 1.015 ± 0.039
0.954TrpThr: 0.954 ± 0.036
1.021TrpVal: 1.021 ± 0.042
0.396TrpTrp: 0.396 ± 0.025
0.313TrpTyr: 0.313 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.252TyrAla: 2.252 ± 0.057
0.129TyrCys: 0.129 ± 0.013
1.436TyrAsp: 1.436 ± 0.049
1.296TyrGlu: 1.296 ± 0.047
0.716TyrPhe: 0.716 ± 0.032
1.889TyrGly: 1.889 ± 0.042
0.39TyrHis: 0.39 ± 0.022
0.792TyrIle: 0.792 ± 0.034
0.492TyrLys: 0.492 ± 0.026
2.211TyrLeu: 2.211 ± 0.052
0.279TyrMet: 0.279 ± 0.02
0.591TyrAsn: 0.591 ± 0.029
1.128TyrPro: 1.128 ± 0.04
0.889TyrGln: 0.889 ± 0.032
1.804TyrArg: 1.804 ± 0.052
1.292TyrSer: 1.292 ± 0.04
1.298TyrThr: 1.298 ± 0.053
1.816TyrVal: 1.816 ± 0.052
0.321TyrTrp: 0.321 ± 0.021
0.536TyrTyr: 0.536 ± 0.027
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2348 proteins (839289 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski