Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Staphylococcaceae; Staphylococcus; Staphylococcus saprophyticus

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2404 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q4A0Y1|Q4A0Y1_STAS1 UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase GtfA subunit OS=Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41) OX=342451 GN=gtfA PE=3 SV=1
MM1 pKa = 7.61KK2 pKa = 9.12KK3 pKa = 8.51TVVASTLAVGLGVTGFAAGNSADD26 pKa = 3.73ASEE29 pKa = 4.26QGVDD33 pKa = 3.3KK34 pKa = 11.21AQLAQQAQSNPEE46 pKa = 4.1SLNAAPVQDD55 pKa = 3.48GAYY58 pKa = 10.29DD59 pKa = 3.67INFNYY64 pKa = 10.6NNTDD68 pKa = 2.93YY69 pKa = 11.33SFQSDD74 pKa = 3.48GQYY77 pKa = 7.48WTWSYY82 pKa = 11.62GQGSTNAPAQEE93 pKa = 4.18TAEE96 pKa = 4.07QPQLVEE102 pKa = 4.22QPQQTEE108 pKa = 4.06QASTEE113 pKa = 4.09QPAQEE118 pKa = 4.44AAPQTEE124 pKa = 4.74EE125 pKa = 3.96TQQPQQEE132 pKa = 4.53ATTQTTSSSNEE143 pKa = 3.89STSNEE148 pKa = 4.07SSSSEE153 pKa = 3.75ASEE156 pKa = 4.14GSSVNVNSHH165 pKa = 6.09LQAIAQRR172 pKa = 11.84EE173 pKa = 4.27SGGDD177 pKa = 3.4LKK179 pKa = 11.18AVNPSSGAAGKK190 pKa = 9.51YY191 pKa = 8.24QFLQSTWDD199 pKa = 3.33SVAPSEE205 pKa = 4.27YY206 pKa = 10.08QGVSPTEE213 pKa = 3.93APEE216 pKa = 4.12AVQDD220 pKa = 3.7AAAVKK225 pKa = 10.25LYY227 pKa = 8.79NTAGASQWVTAA238 pKa = 4.6

Molecular weight: 24.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q49UM5|CYSC_STAS1 Adenylyl-sulfate kinase OS=Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41) OX=342451 GN=cysC PE=3 SV=1
MM1 pKa = 7.49VKK3 pKa = 9.1RR4 pKa = 11.84TYY6 pKa = 10.35QPNKK10 pKa = 8.16RR11 pKa = 11.84KK12 pKa = 9.54HH13 pKa = 5.99SKK15 pKa = 8.87VHH17 pKa = 5.77GFRR20 pKa = 11.84KK21 pKa = 10.02RR22 pKa = 11.84MSTKK26 pKa = 10.18NGRR29 pKa = 11.84KK30 pKa = 8.49VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.05GRR40 pKa = 11.84KK41 pKa = 8.7VLSAA45 pKa = 4.05

Molecular weight: 5.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2404

0

2404

697497

17

2316

290.1

32.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.513 ± 0.049

0.615 ± 0.013

5.757 ± 0.048

6.799 ± 0.073

4.545 ± 0.047

6.281 ± 0.056

2.318 ± 0.026

8.779 ± 0.063

7.089 ± 0.055

9.319 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.724 ± 0.028

5.426 ± 0.046

3.218 ± 0.029

4.074 ± 0.042

3.397 ± 0.034

6.144 ± 0.092

5.652 ± 0.03

6.736 ± 0.042

0.769 ± 0.016

3.844 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski