Amino acid dipepetide frequency for Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.231AlaAla: 4.231 ± 0.111
0.499AlaCys: 0.499 ± 0.029
3.024AlaAsp: 3.024 ± 0.065
3.752AlaGlu: 3.752 ± 0.08
3.253AlaPhe: 3.253 ± 0.095
4.102AlaGly: 4.102 ± 0.091
1.571AlaHis: 1.571 ± 0.046
6.331AlaIle: 6.331 ± 0.11
4.45AlaLys: 4.45 ± 0.099
6.938AlaLeu: 6.938 ± 0.108
1.89AlaMet: 1.89 ± 0.049
3.011AlaAsn: 3.011 ± 0.072
1.831AlaPro: 1.831 ± 0.053
2.74AlaGln: 2.74 ± 0.065
2.173AlaArg: 2.173 ± 0.059
4.093AlaSer: 4.093 ± 0.119
3.736AlaThr: 3.736 ± 0.084
4.592AlaVal: 4.592 ± 0.096
0.489AlaTrp: 0.489 ± 0.03
2.42AlaTyr: 2.42 ± 0.064
0.0AlaXaa: 0.0 ± 0.0
Cys
0.411CysAla: 0.411 ± 0.027
0.075CysCys: 0.075 ± 0.012
0.344CysAsp: 0.344 ± 0.023
0.383CysGlu: 0.383 ± 0.026
0.249CysPhe: 0.249 ± 0.018
0.601CysGly: 0.601 ± 0.032
0.188CysHis: 0.188 ± 0.018
0.604CysIle: 0.604 ± 0.032
0.298CysLys: 0.298 ± 0.022
0.548CysLeu: 0.548 ± 0.027
0.153CysMet: 0.153 ± 0.015
0.257CysAsn: 0.257 ± 0.02
0.277CysPro: 0.277 ± 0.019
0.194CysGln: 0.194 ± 0.017
0.199CysArg: 0.199 ± 0.016
0.377CysSer: 0.377 ± 0.026
0.361CysThr: 0.361 ± 0.024
0.368CysVal: 0.368 ± 0.025
0.036CysTrp: 0.036 ± 0.008
0.227CysTyr: 0.227 ± 0.018
0.0CysXaa: 0.0 ± 0.0
Asp
3.723AspAla: 3.723 ± 0.079
0.333AspCys: 0.333 ± 0.021
3.386AspAsp: 3.386 ± 0.086
4.87AspGlu: 4.87 ± 0.1
2.609AspPhe: 2.609 ± 0.061
3.19AspGly: 3.19 ± 0.078
1.227AspHis: 1.227 ± 0.041
5.371AspIle: 5.371 ± 0.109
4.615AspLys: 4.615 ± 0.103
5.015AspLeu: 5.015 ± 0.09
1.577AspMet: 1.577 ± 0.052
2.975AspAsn: 2.975 ± 0.081
1.576AspPro: 1.576 ± 0.049
2.07AspGln: 2.07 ± 0.058
1.9AspArg: 1.9 ± 0.059
2.88AspSer: 2.88 ± 0.075
2.972AspThr: 2.972 ± 0.068
4.125AspVal: 4.125 ± 0.085
0.467AspTrp: 0.467 ± 0.025
2.69AspTyr: 2.69 ± 0.073
0.0AspXaa: 0.0 ± 0.0
Glu
5.048GluAla: 5.048 ± 0.111
0.264GluCys: 0.264 ± 0.018
4.076GluAsp: 4.076 ± 0.095
4.672GluGlu: 4.672 ± 0.109
2.228GluPhe: 2.228 ± 0.056
3.609GluGly: 3.609 ± 0.069
1.725GluHis: 1.725 ± 0.058
5.551GluIle: 5.551 ± 0.113
5.193GluLys: 5.193 ± 0.102
5.742GluLeu: 5.742 ± 0.105
2.092GluMet: 2.092 ± 0.059
3.936GluAsn: 3.936 ± 0.084
1.815GluPro: 1.815 ± 0.054
3.449GluGln: 3.449 ± 0.087
2.939GluArg: 2.939 ± 0.069
4.36GluSer: 4.36 ± 0.612
4.09GluThr: 4.09 ± 0.08
4.598GluVal: 4.598 ± 0.089
0.556GluTrp: 0.556 ± 0.031
2.043GluTyr: 2.043 ± 0.054
0.0GluXaa: 0.0 ± 0.0
Phe
2.67PheAla: 2.67 ± 0.075
0.323PheCys: 0.323 ± 0.021
2.886PheAsp: 2.886 ± 0.066
3.242PheGlu: 3.242 ± 0.071
2.003PhePhe: 2.003 ± 0.07
3.296PheGly: 3.296 ± 0.079
0.837PheHis: 0.837 ± 0.034
4.562PheIle: 4.562 ± 0.118
3.422PheLys: 3.422 ± 0.072
3.805PheLeu: 3.805 ± 0.087
1.16PheMet: 1.16 ± 0.044
2.915PheAsn: 2.915 ± 0.063
1.24PhePro: 1.24 ± 0.046
1.329PheGln: 1.329 ± 0.043
1.117PheArg: 1.117 ± 0.045
2.913PheSer: 2.913 ± 0.071
2.409PheThr: 2.409 ± 0.053
3.276PheVal: 3.276 ± 0.076
0.338PheTrp: 0.338 ± 0.026
1.708PheTyr: 1.708 ± 0.059
0.0PheXaa: 0.0 ± 0.0
Gly
4.414GlyAla: 4.414 ± 0.108
0.472GlyCys: 0.472 ± 0.028
3.207GlyAsp: 3.207 ± 0.076
3.702GlyGlu: 3.702 ± 0.081
3.105GlyPhe: 3.105 ± 0.082
4.404GlyGly: 4.404 ± 0.114
1.484GlyHis: 1.484 ± 0.044
5.756GlyIle: 5.756 ± 0.113
4.262GlyLys: 4.262 ± 0.098
6.133GlyLeu: 6.133 ± 0.118
1.761GlyMet: 1.761 ± 0.053
2.642GlyAsn: 2.642 ± 0.062
1.622GlyPro: 1.622 ± 0.049
2.313GlyGln: 2.313 ± 0.055
2.179GlyArg: 2.179 ± 0.059
3.589GlySer: 3.589 ± 0.065
3.655GlyThr: 3.655 ± 0.079
4.776GlyVal: 4.776 ± 0.103
0.556GlyTrp: 0.556 ± 0.031
2.779GlyTyr: 2.779 ± 0.065
0.0GlyXaa: 0.0 ± 0.0
His
1.444HisAla: 1.444 ± 0.042
0.148HisCys: 0.148 ± 0.015
1.319HisAsp: 1.319 ± 0.044
1.392HisGlu: 1.392 ± 0.05
1.265HisPhe: 1.265 ± 0.048
1.415HisGly: 1.415 ± 0.045
0.777HisHis: 0.777 ± 0.037
2.001HisIle: 2.001 ± 0.051
1.392HisLys: 1.392 ± 0.051
2.239HisLeu: 2.239 ± 0.064
0.642HisMet: 0.642 ± 0.029
1.114HisAsn: 1.114 ± 0.042
1.029HisPro: 1.029 ± 0.033
0.992HisGln: 0.992 ± 0.038
0.813HisArg: 0.813 ± 0.035
1.233HisSer: 1.233 ± 0.047
1.227HisThr: 1.227 ± 0.04
1.498HisVal: 1.498 ± 0.046
0.204HisTrp: 0.204 ± 0.016
1.038HisTyr: 1.038 ± 0.042
0.0HisXaa: 0.0 ± 0.0
Ile
6.356IleAla: 6.356 ± 0.128
0.645IleCys: 0.645 ± 0.031
5.415IleAsp: 5.415 ± 0.105
6.009IleGlu: 6.009 ± 0.108
3.858IlePhe: 3.858 ± 0.1
6.288IleGly: 6.288 ± 0.127
1.768IleHis: 1.768 ± 0.05
7.822IleIle: 7.822 ± 0.17
5.802IleLys: 5.802 ± 0.115
7.689IleLeu: 7.689 ± 0.147
2.156IleMet: 2.156 ± 0.057
4.647IleAsn: 4.647 ± 0.096
3.137IlePro: 3.137 ± 0.077
3.183IleGln: 3.183 ± 0.069
2.545IleArg: 2.545 ± 0.07
5.474IleSer: 5.474 ± 0.102
4.961IleThr: 4.961 ± 0.1
6.47IleVal: 6.47 ± 0.123
0.546IleTrp: 0.546 ± 0.031
3.024IleTyr: 3.024 ± 0.075
0.0IleXaa: 0.0 ± 0.0
Lys
4.902LysAla: 4.902 ± 0.091
0.301LysCys: 0.301 ± 0.022
4.879LysAsp: 4.879 ± 0.109
5.556LysGlu: 5.556 ± 0.113
2.295LysPhe: 2.295 ± 0.054
4.039LysGly: 4.039 ± 0.087
1.9LysHis: 1.9 ± 0.058
4.827LysIle: 4.827 ± 0.102
5.303LysLys: 5.303 ± 0.123
6.099LysLeu: 6.099 ± 0.097
2.129LysMet: 2.129 ± 0.05
3.867LysAsn: 3.867 ± 0.083
2.221LysPro: 2.221 ± 0.057
3.877LysGln: 3.877 ± 0.089
2.91LysArg: 2.91 ± 0.076
4.303LysSer: 4.303 ± 0.096
3.554LysThr: 3.554 ± 0.074
4.674LysVal: 4.674 ± 0.096
0.592LysTrp: 0.592 ± 0.026
2.665LysTyr: 2.665 ± 0.066
0.0LysXaa: 0.0 ± 0.0
Leu
6.043LeuAla: 6.043 ± 0.115
0.618LeuCys: 0.618 ± 0.031
5.057LeuAsp: 5.057 ± 0.095
5.768LeuGlu: 5.768 ± 0.107
4.615LeuPhe: 4.615 ± 0.105
6.014LeuGly: 6.014 ± 0.109
1.67LeuHis: 1.67 ± 0.054
8.218LeuIle: 8.218 ± 0.174
6.644LeuLys: 6.644 ± 0.106
8.886LeuLeu: 8.886 ± 0.173
2.487LeuMet: 2.487 ± 0.067
5.893LeuAsn: 5.893 ± 0.104
3.609LeuPro: 3.609 ± 0.076
2.986LeuGln: 2.986 ± 0.075
2.806LeuArg: 2.806 ± 0.06
6.89LeuSer: 6.89 ± 0.338
5.581LeuThr: 5.581 ± 0.095
5.696LeuVal: 5.696 ± 0.103
0.614LeuTrp: 0.614 ± 0.033
3.098LeuTyr: 3.098 ± 0.069
0.0LeuXaa: 0.0 ± 0.0
Met
1.815MetAla: 1.815 ± 0.052
0.152MetCys: 0.152 ± 0.014
1.448MetAsp: 1.448 ± 0.043
1.418MetGlu: 1.418 ± 0.052
1.104MetPhe: 1.104 ± 0.045
1.633MetGly: 1.633 ± 0.055
0.568MetHis: 0.568 ± 0.03
2.485MetIle: 2.485 ± 0.059
2.252MetLys: 2.252 ± 0.061
2.472MetLeu: 2.472 ± 0.06
0.885MetMet: 0.885 ± 0.047
1.735MetAsn: 1.735 ± 0.041
1.022MetPro: 1.022 ± 0.041
0.971MetGln: 0.971 ± 0.045
0.986MetArg: 0.986 ± 0.04
1.941MetSer: 1.941 ± 0.057
1.756MetThr: 1.756 ± 0.049
1.571MetVal: 1.571 ± 0.048
0.128MetTrp: 0.128 ± 0.014
0.897MetTyr: 0.897 ± 0.037
0.0MetXaa: 0.0 ± 0.0
Asn
3.384AsnAla: 3.384 ± 0.081
0.27AsnCys: 0.27 ± 0.021
3.494AsnAsp: 3.494 ± 0.077
4.411AsnGlu: 4.411 ± 0.101
2.011AsnPhe: 2.011 ± 0.057
3.189AsnGly: 3.189 ± 0.075
1.584AsnHis: 1.584 ± 0.047
4.535AsnIle: 4.535 ± 0.088
4.284AsnLys: 4.284 ± 0.085
4.321AsnLeu: 4.321 ± 0.077
1.359AsnMet: 1.359 ± 0.045
3.153AsnAsn: 3.153 ± 0.099
1.984AsnPro: 1.984 ± 0.056
2.876AsnGln: 2.876 ± 0.074
1.865AsnArg: 1.865 ± 0.056
2.576AsnSer: 2.576 ± 0.06
2.773AsnThr: 2.773 ± 0.067
3.515AsnVal: 3.515 ± 0.077
0.404AsnTrp: 0.404 ± 0.025
2.272AsnTyr: 2.272 ± 0.066
0.0AsnXaa: 0.0 ± 0.0
Pro
1.666ProAla: 1.666 ± 0.048
0.176ProCys: 0.176 ± 0.015
1.772ProAsp: 1.772 ± 0.056
2.644ProGlu: 2.644 ± 0.075
1.796ProPhe: 1.796 ± 0.058
1.851ProGly: 1.851 ± 0.056
0.797ProHis: 0.797 ± 0.03
2.899ProIle: 2.899 ± 0.073
2.239ProLys: 2.239 ± 0.059
3.047ProLeu: 3.047 ± 0.076
0.85ProMet: 0.85 ± 0.039
1.874ProAsn: 1.874 ± 0.051
0.687ProPro: 0.687 ± 0.031
1.181ProGln: 1.181 ± 0.042
0.915ProArg: 0.915 ± 0.036
2.053ProSer: 2.053 ± 0.055
1.871ProThr: 1.871 ± 0.052
2.258ProVal: 2.258 ± 0.058
0.261ProTrp: 0.261 ± 0.022
1.342ProTyr: 1.342 ± 0.042
0.0ProXaa: 0.0 ± 0.0
Gln
2.637GlnAla: 2.637 ± 0.058
0.212GlnCys: 0.212 ± 0.016
2.082GlnAsp: 2.082 ± 0.061
2.192GlnGlu: 2.192 ± 0.06
2.102GlnPhe: 2.102 ± 0.06
2.191GlnGly: 2.191 ± 0.06
1.039GlnHis: 1.039 ± 0.043
3.179GlnIle: 3.179 ± 0.071
2.585GlnLys: 2.585 ± 0.07
4.206GlnLeu: 4.206 ± 0.086
1.131GlnMet: 1.131 ± 0.041
2.198GlnAsn: 2.198 ± 0.073
1.227GlnPro: 1.227 ± 0.042
2.321GlnGln: 2.321 ± 0.072
1.577GlnArg: 1.577 ± 0.044
2.92GlnSer: 2.92 ± 0.073
2.364GlnThr: 2.364 ± 0.063
2.318GlnVal: 2.318 ± 0.057
0.409GlnTrp: 0.409 ± 0.027
1.852GlnTyr: 1.852 ± 0.054
0.0GlnXaa: 0.0 ± 0.0
Arg
1.986ArgAla: 1.986 ± 0.059
0.158ArgCys: 0.158 ± 0.014
1.915ArgAsp: 1.915 ± 0.065
2.301ArgGlu: 2.301 ± 0.072
1.663ArgPhe: 1.663 ± 0.058
1.921ArgGly: 1.921 ± 0.046
0.834ArgHis: 0.834 ± 0.037
2.929ArgIle: 2.929 ± 0.063
2.595ArgLys: 2.595 ± 0.067
3.272ArgLeu: 3.272 ± 0.07
0.986ArgMet: 0.986 ± 0.039
1.771ArgAsn: 1.771 ± 0.05
1.071ArgPro: 1.071 ± 0.038
1.462ArgGln: 1.462 ± 0.049
1.435ArgArg: 1.435 ± 0.046
1.773ArgSer: 1.773 ± 0.052
1.815ArgThr: 1.815 ± 0.054
2.227ArgVal: 2.227 ± 0.066
0.249ArgTrp: 0.249 ± 0.02
1.601ArgTyr: 1.601 ± 0.055
0.0ArgXaa: 0.0 ± 0.0
Ser
3.538SerAla: 3.538 ± 0.127
0.327SerCys: 0.327 ± 0.021
3.449SerAsp: 3.449 ± 0.076
4.84SerGlu: 4.84 ± 0.608
3.061SerPhe: 3.061 ± 0.073
3.966SerGly: 3.966 ± 0.086
1.366SerHis: 1.366 ± 0.044
5.432SerIle: 5.432 ± 0.098
4.303SerLys: 4.303 ± 0.088
5.931SerLeu: 5.931 ± 0.363
1.679SerMet: 1.679 ± 0.052
3.355SerAsn: 3.355 ± 0.078
1.821SerPro: 1.821 ± 0.061
2.321SerGln: 2.321 ± 0.063
2.042SerArg: 2.042 ± 0.051
3.71SerSer: 3.71 ± 0.091
3.527SerThr: 3.527 ± 0.081
3.858SerVal: 3.858 ± 0.076
0.487SerTrp: 0.487 ± 0.03
2.426SerTyr: 2.426 ± 0.064
0.0SerXaa: 0.0 ± 0.0
Thr
3.458ThrAla: 3.458 ± 0.07
0.366ThrCys: 0.366 ± 0.027
3.047ThrAsp: 3.047 ± 0.07
3.616ThrGlu: 3.616 ± 0.09
2.803ThrPhe: 2.803 ± 0.064
3.768ThrGly: 3.768 ± 0.066
1.385ThrHis: 1.385 ± 0.044
5.091ThrIle: 5.091 ± 0.096
3.521ThrLys: 3.521 ± 0.081
5.784ThrLeu: 5.784 ± 0.091
1.368ThrMet: 1.368 ± 0.041
2.81ThrAsn: 2.81 ± 0.064
2.227ThrPro: 2.227 ± 0.055
2.145ThrGln: 2.145 ± 0.06
1.715ThrArg: 1.715 ± 0.05
3.492ThrSer: 3.492 ± 0.08
3.285ThrThr: 3.285 ± 0.068
3.92ThrVal: 3.92 ± 0.086
0.454ThrTrp: 0.454 ± 0.023
2.27ThrTyr: 2.27 ± 0.06
0.0ThrXaa: 0.0 ± 0.0
Val
4.637ValAla: 4.637 ± 0.092
0.51ValCys: 0.51 ± 0.03
3.914ValAsp: 3.914 ± 0.073
4.386ValGlu: 4.386 ± 0.091
2.89ValPhe: 2.89 ± 0.08
4.492ValGly: 4.492 ± 0.106
1.299ValHis: 1.299 ± 0.044
6.317ValIle: 6.317 ± 0.122
4.681ValLys: 4.681 ± 0.084
6.206ValLeu: 6.206 ± 0.103
1.771ValMet: 1.771 ± 0.051
3.619ValAsn: 3.619 ± 0.071
2.356ValPro: 2.356 ± 0.067
2.232ValGln: 2.232 ± 0.054
2.079ValArg: 2.079 ± 0.057
4.336ValSer: 4.336 ± 0.076
4.118ValThr: 4.118 ± 0.074
4.856ValVal: 4.856 ± 0.095
0.47ValTrp: 0.47 ± 0.025
2.191ValTyr: 2.191 ± 0.052
0.0ValXaa: 0.0 ± 0.0
Trp
0.397TrpAla: 0.397 ± 0.025
0.057TrpCys: 0.057 ± 0.009
0.354TrpAsp: 0.354 ± 0.025
0.338TrpGlu: 0.338 ± 0.024
0.528TrpPhe: 0.528 ± 0.032
0.45TrpGly: 0.45 ± 0.026
0.198TrpHis: 0.198 ± 0.016
0.747TrpIle: 0.747 ± 0.038
0.422TrpLys: 0.422 ± 0.025
1.025TrpLeu: 1.025 ± 0.041
0.272TrpMet: 0.272 ± 0.019
0.396TrpAsn: 0.396 ± 0.026
0.201TrpPro: 0.201 ± 0.02
0.325TrpGln: 0.325 ± 0.021
0.28TrpArg: 0.28 ± 0.019
0.509TrpSer: 0.509 ± 0.03
0.383TrpThr: 0.383 ± 0.021
0.434TrpVal: 0.434 ± 0.029
0.077TrpTrp: 0.077 ± 0.011
0.298TrpTyr: 0.298 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.368TyrAla: 2.368 ± 0.062
0.245TyrCys: 0.245 ± 0.021
2.503TyrAsp: 2.503 ± 0.068
2.495TyrGlu: 2.495 ± 0.059
2.01TyrPhe: 2.01 ± 0.067
2.393TyrGly: 2.393 ± 0.063
0.965TyrHis: 0.965 ± 0.041
3.137TyrIle: 3.137 ± 0.071
2.628TyrLys: 2.628 ± 0.068
3.835TyrLeu: 3.835 ± 0.083
0.889TyrMet: 0.889 ± 0.038
2.098TyrAsn: 2.098 ± 0.06
1.229TyrPro: 1.229 ± 0.037
1.775TyrGln: 1.775 ± 0.059
1.491TyrArg: 1.491 ± 0.045
2.016TyrSer: 2.016 ± 0.051
2.085TyrThr: 2.085 ± 0.053
2.327TyrVal: 2.327 ± 0.064
0.353TyrTrp: 0.353 ± 0.022
1.604TyrTyr: 1.604 ± 0.056
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2404 proteins (697497 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski