Amino acid dipepetide frequency for Sphingomonas sp. Leaf339

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.004AlaAla: 21.004 ± 0.237	0.972AlaCys: 0.972 ± 0.032	8.365AlaAsp: 8.365 ± 0.101	6.742AlaGlu: 6.742 ± 0.089	4.356AlaPhe: 4.356 ± 0.072	12.481AlaGly: 12.481 ± 0.121	2.251AlaHis: 2.251 ± 0.045	7.346AlaIle: 7.346 ± 0.087	3.711AlaLys: 3.711 ± 0.072	13.509AlaLeu: 13.509 ± 0.145	4.067AlaMet: 4.067 ± 0.065	3.292AlaAsn: 3.292 ± 0.056	6.5AlaPro: 6.5 ± 0.103	4.337AlaGln: 4.337 ± 0.073	9.779AlaArg: 9.779 ± 0.119	6.445AlaSer: 6.445 ± 0.084	8.442AlaThr: 8.442 ± 0.093	9.475AlaVal: 9.475 ± 0.108	1.734AlaTrp: 1.734 ± 0.043	2.556AlaTyr: 2.556 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	0.861CysAla: 0.861 ± 0.032	0.078CysCys: 0.078 ± 0.01	0.478CysAsp: 0.478 ± 0.018	0.292CysGlu: 0.292 ± 0.017	0.256CysPhe: 0.256 ± 0.014	0.762CysGly: 0.762 ± 0.026	0.175CysHis: 0.175 ± 0.013	0.326CysIle: 0.326 ± 0.018	0.122CysLys: 0.122 ± 0.011	0.6CysLeu: 0.6 ± 0.025	0.122CysMet: 0.122 ± 0.011	0.184CysAsn: 0.184 ± 0.012	0.394CysPro: 0.394 ± 0.021	0.169CysGln: 0.169 ± 0.012	0.551CysArg: 0.551 ± 0.021	0.378CysSer: 0.378 ± 0.017	0.374CysThr: 0.374 ± 0.018	0.489CysVal: 0.489 ± 0.021	0.099CysTrp: 0.099 ± 0.009	0.15CysTyr: 0.15 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	8.358AspAla: 8.358 ± 0.093	0.415AspCys: 0.415 ± 0.022	3.66AspAsp: 3.66 ± 0.071	3.142AspGlu: 3.142 ± 0.062	2.002AspPhe: 2.002 ± 0.045	6.039AspGly: 6.039 ± 0.082	1.321AspHis: 1.321 ± 0.036	2.808AspIle: 2.808 ± 0.057	1.521AspLys: 1.521 ± 0.042	5.822AspLeu: 5.822 ± 0.081	1.273AspMet: 1.273 ± 0.037	1.365AspAsn: 1.365 ± 0.041	4.01AspPro: 4.01 ± 0.054	1.972AspGln: 1.972 ± 0.04	5.613AspArg: 5.613 ± 0.079	2.071AspSer: 2.071 ± 0.053	3.208AspThr: 3.208 ± 0.05	4.437AspVal: 4.437 ± 0.065	1.209AspTrp: 1.209 ± 0.038	1.643AspTyr: 1.643 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	6.628GluAla: 6.628 ± 0.093	0.232GluCys: 0.232 ± 0.015	2.246GluAsp: 2.246 ± 0.048	2.214GluGlu: 2.214 ± 0.058	1.27GluPhe: 1.27 ± 0.036	3.996GluGly: 3.996 ± 0.063	1.029GluHis: 1.029 ± 0.034	2.349GluIle: 2.349 ± 0.054	1.363GluLys: 1.363 ± 0.042	4.277GluLeu: 4.277 ± 0.067	1.176GluMet: 1.176 ± 0.035	1.112GluAsn: 1.112 ± 0.036	2.397GluPro: 2.397 ± 0.051	1.854GluGln: 1.854 ± 0.045	4.678GluArg: 4.678 ± 0.063	1.82GluSer: 1.82 ± 0.042	2.864GluThr: 2.864 ± 0.051	3.3GluVal: 3.3 ± 0.051	0.728GluTrp: 0.728 ± 0.026	0.886GluTyr: 0.886 ± 0.03	0.001GluXaa: 0.001 ± 0.001
Phe	5.061PheAla: 5.061 ± 0.068	0.264PheCys: 0.264 ± 0.015	2.768PheAsp: 2.768 ± 0.052	1.625PheGlu: 1.625 ± 0.034	1.211PhePhe: 1.211 ± 0.032	3.645PheGly: 3.645 ± 0.061	0.706PheHis: 0.706 ± 0.025	1.261PheIle: 1.261 ± 0.038	0.707PheLys: 0.707 ± 0.024	2.95PheLeu: 2.95 ± 0.064	0.705PheMet: 0.705 ± 0.026	1.021PheAsn: 1.021 ± 0.037	1.453PhePro: 1.453 ± 0.036	0.946PheGln: 0.946 ± 0.033	2.273PheArg: 2.273 ± 0.045	1.804PheSer: 1.804 ± 0.044	2.203PheThr: 2.203 ± 0.049	2.726PheVal: 2.726 ± 0.05	0.494PheTrp: 0.494 ± 0.024	0.914PheTyr: 0.914 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	10.242GlyAla: 10.242 ± 0.097	0.77GlyCys: 0.77 ± 0.026	5.271GlyAsp: 5.271 ± 0.073	4.538GlyGlu: 4.538 ± 0.076	3.762GlyPhe: 3.762 ± 0.065	8.573GlyGly: 8.573 ± 0.112	1.855GlyHis: 1.855 ± 0.042	4.697GlyIle: 4.697 ± 0.071	2.905GlyLys: 2.905 ± 0.059	8.525GlyLeu: 8.525 ± 0.086	2.277GlyMet: 2.277 ± 0.05	2.379GlyAsn: 2.379 ± 0.054	3.695GlyPro: 3.695 ± 0.062	3.066GlyGln: 3.066 ± 0.055	7.089GlyArg: 7.089 ± 0.078	4.911GlySer: 4.911 ± 0.064	5.387GlyThr: 5.387 ± 0.079	6.745GlyVal: 6.745 ± 0.089	1.751GlyTrp: 1.751 ± 0.042	2.527GlyTyr: 2.527 ± 0.042	0.001GlyXaa: 0.001 ± 0.001
His	2.444HisAla: 2.444 ± 0.052	0.183HisCys: 0.183 ± 0.014	1.332HisAsp: 1.332 ± 0.035	0.921HisGlu: 0.921 ± 0.028	0.704HisPhe: 0.704 ± 0.022	1.934HisGly: 1.934 ± 0.044	0.557HisHis: 0.557 ± 0.025	0.798HisIle: 0.798 ± 0.026	0.352HisLys: 0.352 ± 0.018	1.886HisLeu: 1.886 ± 0.044	0.37HisMet: 0.37 ± 0.018	0.398HisAsn: 0.398 ± 0.02	1.276HisPro: 1.276 ± 0.035	0.539HisGln: 0.539 ± 0.023	1.539HisArg: 1.539 ± 0.037	0.773HisSer: 0.773 ± 0.027	0.735HisThr: 0.735 ± 0.026	1.485HisVal: 1.485 ± 0.036	0.37HisTrp: 0.37 ± 0.02	0.508HisTyr: 0.508 ± 0.021	0.001HisXaa: 0.001 ± 0.001
Ile	8.26IleAla: 8.26 ± 0.104	0.354IleCys: 0.354 ± 0.022	4.351IleAsp: 4.351 ± 0.065	3.13IleGlu: 3.13 ± 0.058	1.38IlePhe: 1.38 ± 0.034	5.436IleGly: 5.436 ± 0.078	0.82IleHis: 0.82 ± 0.025	1.761IleIle: 1.761 ± 0.042	1.031IleLys: 1.031 ± 0.034	3.672IleLeu: 3.672 ± 0.067	0.836IleMet: 0.836 ± 0.032	1.249IleAsn: 1.249 ± 0.035	2.155IlePro: 2.155 ± 0.044	1.084IleGln: 1.084 ± 0.035	3.27IleArg: 3.27 ± 0.053	2.232IleSer: 2.232 ± 0.053	2.615IleThr: 2.615 ± 0.052	4.598IleVal: 4.598 ± 0.074	0.589IleTrp: 0.589 ± 0.025	0.94IleTyr: 0.94 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	3.415LysAla: 3.415 ± 0.067	0.108LysCys: 0.108 ± 0.011	1.276LysAsp: 1.276 ± 0.043	0.963LysGlu: 0.963 ± 0.039	0.711LysPhe: 0.711 ± 0.028	2.231LysGly: 2.231 ± 0.05	0.419LysHis: 0.419 ± 0.021	1.224LysIle: 1.224 ± 0.035	0.847LysLys: 0.847 ± 0.035	2.79LysLeu: 2.79 ± 0.055	0.613LysMet: 0.613 ± 0.023	0.626LysAsn: 0.626 ± 0.022	1.855LysPro: 1.855 ± 0.046	0.814LysGln: 0.814 ± 0.03	2.043LysArg: 2.043 ± 0.047	1.313LysSer: 1.313 ± 0.038	1.607LysThr: 1.607 ± 0.041	1.919LysVal: 1.919 ± 0.052	0.314LysTrp: 0.314 ± 0.015	0.529LysTyr: 0.529 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	14.229LeuAla: 14.229 ± 0.157	0.678LeuCys: 0.678 ± 0.024	6.149LeuAsp: 6.149 ± 0.084	3.788LeuGlu: 3.788 ± 0.072	3.554LeuPhe: 3.554 ± 0.061	8.22LeuGly: 8.22 ± 0.095	1.757LeuHis: 1.757 ± 0.047	4.742LeuIle: 4.742 ± 0.074	2.622LeuLys: 2.622 ± 0.052	8.989LeuLeu: 8.989 ± 0.133	2.165LeuMet: 2.165 ± 0.047	2.319LeuAsn: 2.319 ± 0.049	5.675LeuPro: 5.675 ± 0.076	2.265LeuGln: 2.265 ± 0.047	6.591LeuArg: 6.591 ± 0.09	5.799LeuSer: 5.799 ± 0.073	6.12LeuThr: 6.12 ± 0.082	7.296LeuVal: 7.296 ± 0.094	1.273LeuTrp: 1.273 ± 0.038	1.954LeuTyr: 1.954 ± 0.043	0.002LeuXaa: 0.002 ± 0.001
Met	3.444MetAla: 3.444 ± 0.056	0.128MetCys: 0.128 ± 0.011	1.051MetAsp: 1.051 ± 0.034	0.866MetGlu: 0.866 ± 0.027	0.637MetPhe: 0.637 ± 0.026	1.773MetGly: 1.773 ± 0.04	0.429MetHis: 0.429 ± 0.018	1.39MetIle: 1.39 ± 0.04	0.85MetLys: 0.85 ± 0.03	2.648MetLeu: 2.648 ± 0.053	0.682MetMet: 0.682 ± 0.027	0.673MetAsn: 0.673 ± 0.024	1.578MetPro: 1.578 ± 0.037	0.674MetGln: 0.674 ± 0.024	1.752MetArg: 1.752 ± 0.037	1.229MetSer: 1.229 ± 0.03	1.899MetThr: 1.899 ± 0.039	1.741MetVal: 1.741 ± 0.038	0.235MetTrp: 0.235 ± 0.015	0.269MetTyr: 0.269 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.217AsnAla: 3.217 ± 0.048	0.221AsnCys: 0.221 ± 0.013	1.551AsnAsp: 1.551 ± 0.048	1.017AsnGlu: 1.017 ± 0.027	0.889AsnPhe: 0.889 ± 0.033	2.548AsnGly: 2.548 ± 0.058	0.465AsnHis: 0.465 ± 0.02	1.219AsnIle: 1.219 ± 0.036	0.531AsnLys: 0.531 ± 0.023	2.349AsnLeu: 2.349 ± 0.055	0.513AsnMet: 0.513 ± 0.023	0.772AsnAsn: 0.772 ± 0.036	1.706AsnPro: 1.706 ± 0.039	0.744AsnGln: 0.744 ± 0.028	1.84AsnArg: 1.84 ± 0.041	1.248AsnSer: 1.248 ± 0.067	1.304AsnThr: 1.304 ± 0.041	1.921AsnVal: 1.921 ± 0.048	0.415AsnTrp: 0.415 ± 0.022	0.747AsnTyr: 0.747 ± 0.029	0.0AsnXaa: 0.0 ± 0.0
Pro	7.835ProAla: 7.835 ± 0.104	0.311ProCys: 0.311 ± 0.017	3.948ProAsp: 3.948 ± 0.062	2.735ProGlu: 2.735 ± 0.053	1.906ProPhe: 1.906 ± 0.041	4.79ProGly: 4.79 ± 0.062	1.043ProHis: 1.043 ± 0.033	2.665ProIle: 2.665 ± 0.046	1.305ProLys: 1.305 ± 0.039	4.974ProLeu: 4.974 ± 0.073	1.248ProMet: 1.248 ± 0.034	1.281ProAsn: 1.281 ± 0.039	2.852ProPro: 2.852 ± 0.08	1.538ProGln: 1.538 ± 0.042	3.202ProArg: 3.202 ± 0.056	2.665ProSer: 2.665 ± 0.051	3.15ProThr: 3.15 ± 0.058	4.742ProVal: 4.742 ± 0.079	0.714ProTrp: 0.714 ± 0.025	1.135ProTyr: 1.135 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	4.055GlnAla: 4.055 ± 0.065	0.18GlnCys: 0.18 ± 0.013	1.378GlnAsp: 1.378 ± 0.039	1.062GlnGlu: 1.062 ± 0.032	1.075GlnPhe: 1.075 ± 0.035	2.428GlnGly: 2.428 ± 0.051	0.575GlnHis: 0.575 ± 0.021	1.62GlnIle: 1.62 ± 0.04	0.72GlnLys: 0.72 ± 0.027	2.932GlnLeu: 2.932 ± 0.051	0.801GlnMet: 0.801 ± 0.03	0.771GlnAsn: 0.771 ± 0.029	1.889GlnPro: 1.889 ± 0.045	1.102GlnGln: 1.102 ± 0.035	2.537GlnArg: 2.537 ± 0.047	1.679GlnSer: 1.679 ± 0.033	1.834GlnThr: 1.834 ± 0.044	2.409GlnVal: 2.409 ± 0.051	0.414GlnTrp: 0.414 ± 0.019	0.625GlnTyr: 0.625 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	8.955ArgAla: 8.955 ± 0.102	0.475ArgCys: 0.475 ± 0.024	4.589ArgAsp: 4.589 ± 0.069	3.429ArgGlu: 3.429 ± 0.057	3.359ArgPhe: 3.359 ± 0.06	5.511ArgGly: 5.511 ± 0.077	1.751ArgHis: 1.751 ± 0.044	4.205ArgIle: 4.205 ± 0.058	1.621ArgLys: 1.621 ± 0.043	8.399ArgLeu: 8.399 ± 0.109	2.034ArgMet: 2.034 ± 0.044	1.815ArgAsn: 1.815 ± 0.045	3.948ArgPro: 3.948 ± 0.07	2.619ArgGln: 2.619 ± 0.049	6.367ArgArg: 6.367 ± 0.093	3.721ArgSer: 3.721 ± 0.062	4.042ArgThr: 4.042 ± 0.054	5.252ArgVal: 5.252 ± 0.068	1.447ArgTrp: 1.447 ± 0.039	2.053ArgTyr: 2.053 ± 0.05	0.0ArgXaa: 0.0 ± 0.0
Ser	6.195SerAla: 6.195 ± 0.077	0.345SerCys: 0.345 ± 0.019	2.898SerAsp: 2.898 ± 0.051	2.013SerGlu: 2.013 ± 0.04	2.163SerPhe: 2.163 ± 0.042	5.069SerGly: 5.069 ± 0.073	0.85SerHis: 0.85 ± 0.026	2.699SerIle: 2.699 ± 0.049	1.231SerLys: 1.231 ± 0.033	4.813SerLeu: 4.813 ± 0.076	1.142SerMet: 1.142 ± 0.031	1.377SerAsn: 1.377 ± 0.045	2.824SerPro: 2.824 ± 0.054	1.321SerGln: 1.321 ± 0.036	3.363SerArg: 3.363 ± 0.065	2.616SerSer: 2.616 ± 0.054	2.903SerThr: 2.903 ± 0.061	3.849SerVal: 3.849 ± 0.063	0.692SerTrp: 0.692 ± 0.027	1.343SerTyr: 1.343 ± 0.043	0.0SerXaa: 0.0 ± 0.0
Thr	7.69ThrAla: 7.69 ± 0.101	0.356ThrCys: 0.356 ± 0.018	3.375ThrAsp: 3.375 ± 0.055	2.268ThrGlu: 2.268 ± 0.05	1.855ThrPhe: 1.855 ± 0.047	6.086ThrGly: 6.086 ± 0.088	1.009ThrHis: 1.009 ± 0.034	3.417ThrIle: 3.417 ± 0.054	1.347ThrLys: 1.347 ± 0.039	6.488ThrLeu: 6.488 ± 0.076	1.417ThrMet: 1.417 ± 0.038	1.438ThrAsn: 1.438 ± 0.044	3.901ThrPro: 3.901 ± 0.06	1.57ThrGln: 1.57 ± 0.041	4.076ThrArg: 4.076 ± 0.057	2.788ThrSer: 2.788 ± 0.056	3.525ThrThr: 3.525 ± 0.07	4.769ThrVal: 4.769 ± 0.07	0.725ThrTrp: 0.725 ± 0.025	1.341ThrTyr: 1.341 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	11.095ValAla: 11.095 ± 0.126	0.474ValCys: 0.474 ± 0.021	4.772ValAsp: 4.772 ± 0.059	4.137ValGlu: 4.137 ± 0.066	2.286ValPhe: 2.286 ± 0.041	6.165ValGly: 6.165 ± 0.076	1.29ValHis: 1.29 ± 0.033	3.711ValIle: 3.711 ± 0.06	1.885ValLys: 1.885 ± 0.046	6.749ValLeu: 6.749 ± 0.092	1.778ValMet: 1.778 ± 0.04	2.044ValAsn: 2.044 ± 0.05	4.166ValPro: 4.166 ± 0.063	2.107ValGln: 2.107 ± 0.047	5.437ValArg: 5.437 ± 0.081	4.086ValSer: 4.086 ± 0.067	5.117ValThr: 5.117 ± 0.071	6.118ValVal: 6.118 ± 0.09	0.901ValTrp: 0.901 ± 0.028	1.437ValTyr: 1.437 ± 0.037	0.0ValXaa: 0.0 ± 0.0
Trp	1.48TrpAla: 1.48 ± 0.042	0.121TrpCys: 0.121 ± 0.01	0.748TrpAsp: 0.748 ± 0.027	0.516TrpGlu: 0.516 ± 0.019	0.568TrpPhe: 0.568 ± 0.025	1.034TrpGly: 1.034 ± 0.033	0.355TrpHis: 0.355 ± 0.02	0.692TrpIle: 0.692 ± 0.024	0.413TrpLys: 0.413 ± 0.021	1.724TrpLeu: 1.724 ± 0.045	0.357TrpMet: 0.357 ± 0.014	0.436TrpAsn: 0.436 ± 0.023	0.723TrpPro: 0.723 ± 0.026	0.651TrpGln: 0.651 ± 0.021	1.53TrpArg: 1.53 ± 0.045	0.952TrpSer: 0.952 ± 0.033	0.953TrpThr: 0.953 ± 0.029	0.882TrpVal: 0.882 ± 0.032	0.321TrpTrp: 0.321 ± 0.018	0.334TrpTyr: 0.334 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.892TyrAla: 2.892 ± 0.054	0.195TyrCys: 0.195 ± 0.014	1.684TyrAsp: 1.684 ± 0.042	1.024TyrGlu: 1.024 ± 0.031	0.794TyrPhe: 0.794 ± 0.027	2.245TyrGly: 2.245 ± 0.053	0.447TyrHis: 0.447 ± 0.018	0.835TyrIle: 0.835 ± 0.03	0.533TyrLys: 0.533 ± 0.021	2.09TyrLeu: 2.09 ± 0.042	0.371TyrMet: 0.371 ± 0.017	0.662TyrAsn: 0.662 ± 0.032	1.039TyrPro: 1.039 ± 0.03	0.713TyrGln: 0.713 ± 0.029	2.061TyrArg: 2.061 ± 0.045	1.177TyrSer: 1.177 ± 0.035	1.163TyrThr: 1.163 ± 0.033	1.599TyrVal: 1.599 ± 0.041	0.365TyrTrp: 0.365 ± 0.02	0.626TyrTyr: 0.626 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.002XaaVal: 0.002 ± 0.001	0.001XaaTrp: 0.001 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3477 proteins (1136623 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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