Endocarpon pusillum (strain Z07020 / HMAS-L-300199) (Lichen-forming fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Chaetothyriomycetidae; Verrucariales; Verrucariaceae; Endocarpon; Endocarpon pusillum

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9228 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U1HHU0|U1HHU0_ENDPU MOSC domain-containing protein OS=Endocarpon pusillum (strain Z07020 / HMAS-L-300199) OX=1263415 GN=EPUS_03752 PE=4 SV=1
MM1 pKa = 7.73RR2 pKa = 11.84LLVLVLAALGICATASPIVYY22 pKa = 8.31EE23 pKa = 4.08ASGVAKK29 pKa = 10.32RR30 pKa = 11.84VDD32 pKa = 3.16ADD34 pKa = 3.7EE35 pKa = 4.54AVVYY39 pKa = 10.32PDD41 pKa = 3.27EE42 pKa = 4.07QFYY45 pKa = 11.59AEE47 pKa = 4.16DD48 pKa = 4.12FKK50 pKa = 11.54RR51 pKa = 11.84SVDD54 pKa = 3.33ADD56 pKa = 3.67EE57 pKa = 4.75SVVYY61 pKa = 10.15PDD63 pKa = 3.53EE64 pKa = 4.13EE65 pKa = 5.03FYY67 pKa = 11.48AEE69 pKa = 4.06DD70 pKa = 4.35FKK72 pKa = 11.99YY73 pKa = 10.77CLADD77 pKa = 3.47EE78 pKa = 4.58AVVV81 pKa = 3.28

Molecular weight: 8.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U1G9E1|U1G9E1_ENDPU CCT-epsilon OS=Endocarpon pusillum (strain Z07020 / HMAS-L-300199) OX=1263415 GN=EPUS_07169 PE=3 SV=1
MM1 pKa = 7.16FALHH5 pKa = 5.84VRR7 pKa = 11.84PPRR10 pKa = 11.84SPSTFALHH18 pKa = 5.53VRR20 pKa = 11.84PPRR23 pKa = 11.84SPSTFALHH31 pKa = 5.53VRR33 pKa = 11.84PPRR36 pKa = 11.84SPSTFALHH44 pKa = 5.53VRR46 pKa = 11.84PPRR49 pKa = 11.84SPSTFALHH57 pKa = 5.53VRR59 pKa = 11.84PPRR62 pKa = 11.84SPSTFALHH70 pKa = 5.53VRR72 pKa = 11.84PPRR75 pKa = 11.84SPSTFALHH83 pKa = 5.53VRR85 pKa = 11.84PPRR88 pKa = 11.84SPSTFALHH96 pKa = 5.53VRR98 pKa = 11.84PPRR101 pKa = 11.84SPSTFALHH109 pKa = 5.53VRR111 pKa = 11.84PPRR114 pKa = 11.84SPSTFALHH122 pKa = 5.53VRR124 pKa = 11.84PPRR127 pKa = 11.84SPSTFALHH135 pKa = 5.53VRR137 pKa = 11.84PPRR140 pKa = 11.84SPSTFALHH148 pKa = 5.53VRR150 pKa = 11.84PPRR153 pKa = 11.84SPSTFALHH161 pKa = 5.98VRR163 pKa = 11.84PPQLPTPILFPQAHH177 pKa = 6.77LVTT180 pKa = 4.57

Molecular weight: 20.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9228

0

9228

4890347

50

8000

529.9

58.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.425 ± 0.022

1.208 ± 0.01

5.661 ± 0.018

6.351 ± 0.024

3.524 ± 0.014

6.644 ± 0.02

2.478 ± 0.011

4.831 ± 0.015

4.937 ± 0.018

8.977 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.096 ± 0.008

3.709 ± 0.014

6.033 ± 0.024

4.266 ± 0.017

6.427 ± 0.022

8.649 ± 0.027

5.958 ± 0.017

5.805 ± 0.016

1.372 ± 0.008

2.623 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski