Cucumber mottle virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Virgaviridae; Tobamovirus

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0PA52|A0PA52_9VIRU Isoform of A0PA51 130 kDa replicase OS=Cucumber mottle virus OX=388038 GN=replicase PE=4 SV=1
MM1 pKa = 7.9AYY3 pKa = 10.47SPITPTNLSLFSSNYY18 pKa = 8.82VPFTEE23 pKa = 5.35FYY25 pKa = 10.18NYY27 pKa = 10.6LVTAQGEE34 pKa = 4.36AFQTQQGRR42 pKa = 11.84DD43 pKa = 3.42SVRR46 pKa = 11.84DD47 pKa = 3.6SLSGFFSSPVSPTVRR62 pKa = 11.84FPDD65 pKa = 3.56GVFYY69 pKa = 11.23VFLGNPVLDD78 pKa = 4.34PLFKK82 pKa = 10.9ALLQSLDD89 pKa = 3.02TRR91 pKa = 11.84NRR93 pKa = 11.84VIEE96 pKa = 4.0VDD98 pKa = 3.49NPSNPTTAEE107 pKa = 3.83SLNAVQRR114 pKa = 11.84TDD116 pKa = 3.3DD117 pKa = 3.72STVSSRR123 pKa = 11.84VGLINLRR130 pKa = 11.84AAITQGNGVVNRR142 pKa = 11.84SIFEE146 pKa = 4.15SSNGLTWATSGSSSSKK162 pKa = 10.73

Molecular weight: 17.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0PA54|A0PA54_9VIRU Capsid protein OS=Cucumber mottle virus OX=388038 GN=cp PE=3 SV=1
MM1 pKa = 7.58SISNLSVEE9 pKa = 4.59SQLKK13 pKa = 9.49PSNFVKK19 pKa = 10.73LSWVDD24 pKa = 3.76KK25 pKa = 10.66LLPDD29 pKa = 3.55YY30 pKa = 10.88FSILRR35 pKa = 11.84FLSVTDD41 pKa = 3.5HH42 pKa = 6.36SVIKK46 pKa = 10.49ARR48 pKa = 11.84EE49 pKa = 4.03YY50 pKa = 10.88EE51 pKa = 4.1SFLPVEE57 pKa = 4.47LLRR60 pKa = 11.84GVDD63 pKa = 3.87LTKK66 pKa = 10.6HH67 pKa = 6.03KK68 pKa = 10.85YY69 pKa = 7.96VTLLGVVISGVWTIPEE85 pKa = 4.58GCSGGATVGLVDD97 pKa = 3.4TRR99 pKa = 11.84MEE101 pKa = 4.01RR102 pKa = 11.84VVEE105 pKa = 4.04GTVCKK110 pKa = 10.39FSVPASVRR118 pKa = 11.84EE119 pKa = 3.9FNVRR123 pKa = 11.84FIPNYY128 pKa = 10.46SITAADD134 pKa = 4.09AARR137 pKa = 11.84HH138 pKa = 5.09PWSLFVRR145 pKa = 11.84LKK147 pKa = 10.62GVNIKK152 pKa = 10.87DD153 pKa = 3.64SFSPLTLEE161 pKa = 4.01IAALVATTNSIIKK174 pKa = 9.87KK175 pKa = 7.36SLKK178 pKa = 10.51AIVSDD183 pKa = 3.85VVVGSDD189 pKa = 3.19AAVAIADD196 pKa = 4.42RR197 pKa = 11.84DD198 pKa = 3.95SQVNSFFDD206 pKa = 3.57SVPITKK212 pKa = 10.2SVVNFDD218 pKa = 3.23KK219 pKa = 10.7SYY221 pKa = 10.83KK222 pKa = 10.24SRR224 pKa = 11.84VPKK227 pKa = 10.05KK228 pKa = 10.19DD229 pKa = 3.16SSGGSGKK236 pKa = 9.75VKK238 pKa = 10.5RR239 pKa = 11.84STGTSGPADD248 pKa = 3.57TEE250 pKa = 4.21FSDD253 pKa = 6.06DD254 pKa = 3.81GLLSNHH260 pKa = 6.27SDD262 pKa = 3.01

Molecular weight: 28.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

1

4

3262

162

1672

815.5

91.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.499 ± 0.256

1.594 ± 0.429

6.683 ± 0.324

4.997 ± 0.569

6.101 ± 0.26

4.844 ± 0.469

2.115 ± 0.482

4.966 ± 0.328

6.407 ± 0.91

8.768 ± 0.303

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.931 ± 0.447

4.69 ± 0.461

4.384 ± 0.322

2.606 ± 0.417

4.782 ± 0.173

8.829 ± 1.956

5.917 ± 0.504

9.718 ± 0.698

0.95 ± 0.059

3.188 ± 0.308
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski