Nitratireductor indicus C115

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Nitratireductor; Nitratireductor indicus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4821 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K2NXR5|K2NXR5_9RHIZ Uncharacterized protein OS=Nitratireductor indicus C115 OX=1231190 GN=NA8A_04365 PE=4 SV=1
MM1 pKa = 7.73EE2 pKa = 4.42VRR4 pKa = 11.84QSSGAGIVDD13 pKa = 3.4WNGFSIGSDD22 pKa = 3.09NQVHH26 pKa = 6.2FEE28 pKa = 4.13NGSGATLNRR37 pKa = 11.84VTGNVSSQIDD47 pKa = 3.82GALTATGSVFLINPAGVVVGSGGMVATGGSFVASTHH83 pKa = 6.67DD84 pKa = 3.4VTDD87 pKa = 3.72ADD89 pKa = 4.16FLDD92 pKa = 4.49GGSLIFSGTSEE103 pKa = 4.45AEE105 pKa = 4.06VVNAGTIRR113 pKa = 11.84SQQGDD118 pKa = 3.14IALIARR124 pKa = 11.84RR125 pKa = 11.84VEE127 pKa = 3.89NSGTLEE133 pKa = 4.31APNGTAGLAAGYY145 pKa = 9.31KK146 pKa = 10.25VLMKK150 pKa = 10.68DD151 pKa = 3.07STDD154 pKa = 3.59ADD156 pKa = 3.82GLLSVQLGGEE166 pKa = 4.06DD167 pKa = 3.54TEE169 pKa = 4.72AVNSGTIAAANAEE182 pKa = 3.72IRR184 pKa = 11.84ANGGNVYY191 pKa = 10.71ALAGNTEE198 pKa = 4.37GVIKK202 pKa = 9.99ATGVTSSGGRR212 pKa = 11.84IFLTAGAAGKK222 pKa = 10.56VKK224 pKa = 9.67VTGRR228 pKa = 11.84LKK230 pKa = 11.01ARR232 pKa = 11.84TLPKK236 pKa = 9.2TAPVPTPRR244 pKa = 11.84PATEE248 pKa = 4.57GGHH251 pKa = 4.44VTVSGGDD258 pKa = 3.24ISISGTIDD266 pKa = 2.99ASAEE270 pKa = 4.01TSGTKK275 pKa = 10.34GGTVTAIASSGMSFSGAIEE294 pKa = 4.12AKK296 pKa = 10.26GGAGGSGGFVEE307 pKa = 5.03TSGEE311 pKa = 4.31SISIADD317 pKa = 3.92TARR320 pKa = 11.84VTTLSGGGEE329 pKa = 4.09AGTWLIDD336 pKa = 4.13PNDD339 pKa = 3.68LTIAASGGDD348 pKa = 3.3ITGEE352 pKa = 4.04TLSTNLAGGNVTLSSNDD369 pKa = 3.1GATDD373 pKa = 4.02GSGDD377 pKa = 3.34IFVNDD382 pKa = 5.11AISWTADD389 pKa = 2.91TTLTLNAVRR398 pKa = 11.84NVEE401 pKa = 4.05INDD404 pKa = 4.15TITATGTNAGLALTYY419 pKa = 10.78GGGYY423 pKa = 10.0SFANGGRR430 pKa = 11.84ITLSGDD436 pKa = 3.5SANLSLNGNAYY447 pKa = 10.57ALIHH451 pKa = 6.8DD452 pKa = 4.54VNDD455 pKa = 3.52LQAISGSGLYY465 pKa = 10.58ALAEE469 pKa = 4.52TIDD472 pKa = 3.77ASGTTSWNSGAGFNPISGFSGTLAGLGNTIEE503 pKa = 4.37SLTINRR509 pKa = 11.84PGEE512 pKa = 3.97ATVGMFSSTSGGFRR526 pKa = 11.84DD527 pKa = 4.17FTLSNVSIVSGGSVGALAYY546 pKa = 10.22RR547 pKa = 11.84FNAGQGASNIHH558 pKa = 4.5VTGTITSLGGGGQVGGLVGWSDD580 pKa = 3.23QTAISNSSSTATVTGFGEE598 pKa = 4.22VGGLVGRR605 pKa = 11.84LRR607 pKa = 11.84AASITNSYY615 pKa = 8.46ATGAVTGTTDD625 pKa = 3.37YY626 pKa = 11.62VGGLVGSTSQGGMLTNVYY644 pKa = 10.82ASGNVTGTTNVGGLVGGAILAGAVTATNAYY674 pKa = 8.65WDD676 pKa = 3.83VDD678 pKa = 3.74STGQSTSFAGTGIGNANAFTQSTYY702 pKa = 11.4SGFDD706 pKa = 3.34FTNTWVLIDD715 pKa = 3.73GEE717 pKa = 4.76TRR719 pKa = 11.84PMLQSEE725 pKa = 4.8YY726 pKa = 9.42STTIFTPHH734 pKa = 6.28QLQLMALDD742 pKa = 4.71LGADD746 pKa = 3.68YY747 pKa = 10.73MLGADD752 pKa = 4.91LDD754 pKa = 3.99MTGAFAANSGGYY766 pKa = 9.46YY767 pKa = 10.21GGVWSSAGFDD777 pKa = 3.33PVGINSANPFTGGLDD792 pKa = 3.97GQGHH796 pKa = 6.6TISGLKK802 pKa = 9.22IDD804 pKa = 5.07RR805 pKa = 11.84SGEE808 pKa = 4.11MNVGLLGYY816 pKa = 10.18ADD818 pKa = 4.01NATVSDD824 pKa = 4.04LTLSGGSIAGGQLVGALAGEE844 pKa = 4.52FNGGTITSVSSNISVAGSEE863 pKa = 4.38FVGGLVGASQNFTAISLASASGDD886 pKa = 3.36VSGSGDD892 pKa = 3.55TVGGLIGALFEE903 pKa = 4.53GTLSQSYY910 pKa = 8.07ATGNVSGSSLVGGLVGANGYY930 pKa = 10.76ASGGGTIDD938 pKa = 3.85QSFATGNVTGAGYY951 pKa = 11.09GLGGLVGSNEE961 pKa = 4.06GAISNAYY968 pKa = 10.48AMGNVTSTGASSATGGFVGVVLSSASIEE996 pKa = 3.95NAYY999 pKa = 9.07ATGKK1003 pKa = 8.29VTGAAPVGGFAGSSGADD1020 pKa = 2.98ASAIKK1025 pKa = 9.95SAYY1028 pKa = 8.63WNTEE1032 pKa = 3.36TSGQASGIVAGGGDD1046 pKa = 3.41ITGLTTAGLQGVLPSGFSNAVWGTGTDD1073 pKa = 3.91LYY1075 pKa = 10.51PYY1077 pKa = 9.92FNWLYY1082 pKa = 8.02PTGASAVSGFAYY1094 pKa = 10.57SDD1096 pKa = 3.4AGTTALTGAEE1106 pKa = 3.86IGIVSGGSFLGGTGTGANGYY1126 pKa = 9.38YY1127 pKa = 10.27YY1128 pKa = 11.17YY1129 pKa = 11.05LALPGALYY1137 pKa = 10.78ASGALAFLDD1146 pKa = 4.21GEE1148 pKa = 4.5AMQGAALGDD1157 pKa = 4.26SVTSTGVTGLDD1168 pKa = 3.13IYY1170 pKa = 11.19GSSLNLVTNEE1180 pKa = 3.91AALSDD1185 pKa = 3.8TVANLTTALGSYY1197 pKa = 9.21TDD1199 pKa = 3.97SDD1201 pKa = 4.23LAFIDD1206 pKa = 4.16TSATQLTASGQGLYY1220 pKa = 10.6LDD1222 pKa = 4.45AASDD1226 pKa = 3.79YY1227 pKa = 11.25TLDD1230 pKa = 3.58QDD1232 pKa = 4.08LTAGGLLSLTSGGTFTIDD1250 pKa = 3.69ADD1252 pKa = 4.24RR1253 pKa = 11.84TLKK1256 pKa = 8.03TTDD1259 pKa = 3.08AGSILVNSGVTWNGTGTLTLNAADD1283 pKa = 4.49NIALNGAII1291 pKa = 4.58

Molecular weight: 126.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K2NPJ2|K2NPJ2_9RHIZ Uncharacterized protein OS=Nitratireductor indicus C115 OX=1231190 GN=NA8A_24236 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.09QPSKK9 pKa = 8.8IVRR12 pKa = 11.84KK13 pKa = 9.24RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84RR29 pKa = 11.84VIAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4821

0

4821

1483311

32

1972

307.7

33.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.185 ± 0.043

0.823 ± 0.011

5.509 ± 0.032

6.182 ± 0.039

3.917 ± 0.023

8.674 ± 0.035

1.958 ± 0.018

5.455 ± 0.03

3.369 ± 0.029

10.075 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.651 ± 0.016

2.672 ± 0.017

4.942 ± 0.023

2.942 ± 0.021

7.07 ± 0.037

5.581 ± 0.027

5.152 ± 0.027

7.38 ± 0.027

1.257 ± 0.013

2.206 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski