Valsa sordida

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Sordariomycetidae; Diaporthales; Valsaceae; Valsa

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9818 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A423WCN8|A0A423WCN8_9PEZI Uncharacterized protein OS=Valsa sordida OX=252740 GN=VSDG_02845 PE=4 SV=1
MM1 pKa = 7.96PSTITTAGVLAFMSLSGALAATNCTFSGSSGFSEE35 pKa = 4.42ISAKK39 pKa = 10.33KK40 pKa = 9.89SSCSTIIIDD49 pKa = 4.18SLEE52 pKa = 4.22VPAGEE57 pKa = 4.2TLDD60 pKa = 5.86LEE62 pKa = 4.57DD63 pKa = 5.93LNDD66 pKa = 3.74FTTLTSNKK74 pKa = 9.82VIFQGQTTWAYY85 pKa = 10.59AEE87 pKa = 4.34WEE89 pKa = 4.42GSLISISGNNITVKK103 pKa = 10.92GEE105 pKa = 3.95AGSALDD111 pKa = 4.03GQGALWWDD119 pKa = 3.5GLGGNGGVTKK129 pKa = 10.56PKK131 pKa = 10.16FFKK134 pKa = 10.81ANKK137 pKa = 9.96LYY139 pKa = 10.95DD140 pKa = 3.76SVLDD144 pKa = 5.1GITILNAPKK153 pKa = 10.49NSFSVNYY160 pKa = 10.06VEE162 pKa = 6.1NLVLQNIVINNTAGDD177 pKa = 4.08EE178 pKa = 4.19LNSDD182 pKa = 4.34GKK184 pKa = 8.85TLGHH188 pKa = 6.01NTDD191 pKa = 4.18AFDD194 pKa = 4.58INNCDD199 pKa = 2.99GVLFQNITVYY209 pKa = 10.99NQDD212 pKa = 3.01DD213 pKa = 4.16CVAVNSGEE221 pKa = 4.02NVVFRR226 pKa = 11.84DD227 pKa = 3.78ALCSGGHH234 pKa = 6.67GISIGSVGGRR244 pKa = 11.84SNNVVNNITFDD255 pKa = 3.54NVLMQNSQQSVRR267 pKa = 11.84IKK269 pKa = 10.09TIADD273 pKa = 3.51TNGTVSNVTYY283 pKa = 10.49RR284 pKa = 11.84NIVIDD289 pKa = 4.22SPTDD293 pKa = 3.25NTDD296 pKa = 2.85YY297 pKa = 11.44GIIVSQSYY305 pKa = 10.77NGVDD309 pKa = 3.62GEE311 pKa = 4.39PTNGVIISNFVLQNVTGTVYY331 pKa = 10.88EE332 pKa = 4.19DD333 pKa = 4.61AINIYY338 pKa = 9.48IEE340 pKa = 4.62CGEE343 pKa = 4.52GSCIDD348 pKa = 3.69WTWTDD353 pKa = 3.49VNVSGGKK360 pKa = 10.1DD361 pKa = 3.0SADD364 pKa = 3.54CMNIPDD370 pKa = 5.8GISCC374 pKa = 4.99

Molecular weight: 39.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A423WNU7|A0A423WNU7_9PEZI Uncharacterized protein OS=Valsa sordida OX=252740 GN=VSDG_00025 PE=4 SV=1
MM1 pKa = 7.55PASIHH6 pKa = 6.89CEE8 pKa = 3.56MAQCQPLCPEE18 pKa = 5.44DD19 pKa = 5.42DD20 pKa = 5.01LISTLIAAASHH31 pKa = 7.23RR32 pKa = 11.84YY33 pKa = 7.04TPAAPSPLNPRR44 pKa = 11.84SSIEE48 pKa = 3.86PKK50 pKa = 9.75SRR52 pKa = 11.84KK53 pKa = 8.74HH54 pKa = 5.88APHH57 pKa = 6.41HH58 pKa = 5.49RR59 pKa = 11.84RR60 pKa = 11.84PRR62 pKa = 11.84SSRR65 pKa = 11.84RR66 pKa = 11.84KK67 pKa = 8.26PAEE70 pKa = 3.93TPTEE74 pKa = 3.63RR75 pKa = 11.84LLRR78 pKa = 11.84RR79 pKa = 11.84KK80 pKa = 9.39AALAYY85 pKa = 9.47EE86 pKa = 4.27RR87 pKa = 11.84TTRR90 pKa = 11.84LSPSLSPTHH99 pKa = 5.92STPPPPPPPRR109 pKa = 11.84RR110 pKa = 11.84KK111 pKa = 10.1NGGTVVAARR120 pKa = 11.84PCSSPEE126 pKa = 3.6KK127 pKa = 10.45GPAISCVANEE137 pKa = 4.16ARR139 pKa = 11.84RR140 pKa = 11.84AGQTRR145 pKa = 11.84DD146 pKa = 3.14KK147 pKa = 10.44PLAVEE152 pKa = 3.81WWEE155 pKa = 3.66IRR157 pKa = 11.84HH158 pKa = 6.16RR159 pKa = 11.84LLADD163 pKa = 3.43VEE165 pKa = 4.43GQPAAQLSGVRR176 pKa = 11.84QRR178 pKa = 11.84PASRR182 pKa = 11.84QRR184 pKa = 11.84LEE186 pKa = 3.84ILLMLVLLSACLTLLTVVGVDD207 pKa = 3.52SLKK210 pKa = 10.39GHH212 pKa = 6.76YY213 pKa = 9.41IAGG216 pKa = 3.81

Molecular weight: 23.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9818

0

9818

4954655

50

5484

504.7

55.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.151 ± 0.027

1.151 ± 0.009

5.993 ± 0.018

6.249 ± 0.024

3.535 ± 0.014

7.422 ± 0.025

2.334 ± 0.01

4.496 ± 0.015

4.707 ± 0.024

8.596 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.294 ± 0.009

3.503 ± 0.012

6.14 ± 0.026

3.976 ± 0.017

6.126 ± 0.021

7.956 ± 0.029

5.915 ± 0.016

6.282 ± 0.02

1.457 ± 0.008

2.715 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski