Amino acid dipepetide frequency for Pseudomonas phage AAT-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.841AlaAla: 12.841 ± 1.31
1.07AlaCys: 1.07 ± 0.305
6.252AlaAsp: 6.252 ± 0.78
8.843AlaGlu: 8.843 ± 0.979
3.436AlaPhe: 3.436 ± 0.511
8.448AlaGly: 8.448 ± 0.73
2.253AlaHis: 2.253 ± 0.378
3.379AlaIle: 3.379 ± 0.418
7.491AlaLys: 7.491 ± 1.322
9.856AlaLeu: 9.856 ± 0.853
3.098AlaMet: 3.098 ± 0.385
3.154AlaAsn: 3.154 ± 0.477
4.112AlaPro: 4.112 ± 0.632
3.83AlaGln: 3.83 ± 0.51
6.984AlaArg: 6.984 ± 0.671
7.209AlaSer: 7.209 ± 0.919
6.928AlaThr: 6.928 ± 0.591
7.829AlaVal: 7.829 ± 0.546
2.14AlaTrp: 2.14 ± 0.325
3.098AlaTyr: 3.098 ± 0.436
0.0AlaXaa: 0.0 ± 0.0
Cys
1.014CysAla: 1.014 ± 0.301
0.169CysCys: 0.169 ± 0.085
0.62CysAsp: 0.62 ± 0.222
0.507CysGlu: 0.507 ± 0.189
0.282CysPhe: 0.282 ± 0.113
1.014CysGly: 1.014 ± 0.343
0.563CysHis: 0.563 ± 0.193
0.169CysIle: 0.169 ± 0.101
0.507CysLys: 0.507 ± 0.182
0.901CysLeu: 0.901 ± 0.236
0.282CysMet: 0.282 ± 0.123
0.225CysAsn: 0.225 ± 0.104
0.789CysPro: 0.789 ± 0.221
0.282CysGln: 0.282 ± 0.138
1.014CysArg: 1.014 ± 0.197
0.563CysSer: 0.563 ± 0.199
0.507CysThr: 0.507 ± 0.184
0.732CysVal: 0.732 ± 0.212
0.282CysTrp: 0.282 ± 0.129
0.62CysTyr: 0.62 ± 0.186
0.0CysXaa: 0.0 ± 0.0
Asp
6.646AspAla: 6.646 ± 0.63
0.394AspCys: 0.394 ± 0.181
4.112AspAsp: 4.112 ± 0.511
5.407AspGlu: 5.407 ± 0.548
2.703AspPhe: 2.703 ± 0.402
7.435AspGly: 7.435 ± 0.641
0.957AspHis: 0.957 ± 0.221
1.69AspIle: 1.69 ± 0.301
2.929AspLys: 2.929 ± 0.392
5.52AspLeu: 5.52 ± 0.54
1.521AspMet: 1.521 ± 0.31
1.352AspAsn: 1.352 ± 0.239
3.21AspPro: 3.21 ± 0.543
2.197AspGln: 2.197 ± 0.399
3.83AspArg: 3.83 ± 0.48
3.21AspSer: 3.21 ± 0.413
1.915AspThr: 1.915 ± 0.319
5.013AspVal: 5.013 ± 0.49
1.69AspTrp: 1.69 ± 0.413
1.521AspTyr: 1.521 ± 0.274
0.0AspXaa: 0.0 ± 0.0
Glu
10.363GluAla: 10.363 ± 1.052
1.014GluCys: 1.014 ± 0.314
4.562GluAsp: 4.562 ± 0.613
3.999GluGlu: 3.999 ± 0.522
1.802GluPhe: 1.802 ± 0.332
5.97GluGly: 5.97 ± 0.489
1.126GluHis: 1.126 ± 0.273
1.239GluIle: 1.239 ± 0.252
3.436GluLys: 3.436 ± 0.59
8.336GluLeu: 8.336 ± 0.576
2.478GluMet: 2.478 ± 0.343
1.859GluAsn: 1.859 ± 0.348
2.929GluPro: 2.929 ± 0.46
2.14GluGln: 2.14 ± 0.503
4.675GluArg: 4.675 ± 0.607
2.253GluSer: 2.253 ± 0.398
2.197GluThr: 2.197 ± 0.342
5.463GluVal: 5.463 ± 0.506
1.126GluTrp: 1.126 ± 0.192
1.802GluTyr: 1.802 ± 0.247
0.0GluXaa: 0.0 ± 0.0
Phe
2.816PheAla: 2.816 ± 0.343
0.282PheCys: 0.282 ± 0.107
2.872PheAsp: 2.872 ± 0.48
2.197PheGlu: 2.197 ± 0.281
1.239PhePhe: 1.239 ± 0.35
2.872PheGly: 2.872 ± 0.363
0.394PheHis: 0.394 ± 0.148
0.845PheIle: 0.845 ± 0.198
1.295PheLys: 1.295 ± 0.262
2.309PheLeu: 2.309 ± 0.351
0.845PheMet: 0.845 ± 0.26
1.408PheAsn: 1.408 ± 0.287
1.746PhePro: 1.746 ± 0.275
1.352PheGln: 1.352 ± 0.253
2.478PheArg: 2.478 ± 0.364
1.352PheSer: 1.352 ± 0.29
1.971PheThr: 1.971 ± 0.29
2.591PheVal: 2.591 ± 0.367
0.507PheTrp: 0.507 ± 0.191
0.676PheTyr: 0.676 ± 0.199
0.0PheXaa: 0.0 ± 0.0
Gly
7.04GlyAla: 7.04 ± 0.68
1.295GlyCys: 1.295 ± 0.334
4.562GlyAsp: 4.562 ± 0.528
5.351GlyGlu: 5.351 ± 0.56
2.76GlyPhe: 2.76 ± 0.434
6.083GlyGly: 6.083 ± 0.664
1.971GlyHis: 1.971 ± 0.304
2.76GlyIle: 2.76 ± 0.425
4.675GlyLys: 4.675 ± 0.588
7.435GlyLeu: 7.435 ± 0.716
2.309GlyMet: 2.309 ± 0.324
2.028GlyAsn: 2.028 ± 0.322
3.436GlyPro: 3.436 ± 0.516
2.872GlyGln: 2.872 ± 0.327
7.491GlyArg: 7.491 ± 0.868
4.956GlySer: 4.956 ± 0.681
4.618GlyThr: 4.618 ± 0.696
7.04GlyVal: 7.04 ± 0.516
1.69GlyTrp: 1.69 ± 0.36
2.816GlyTyr: 2.816 ± 0.58
0.0GlyXaa: 0.0 ± 0.0
His
2.703HisAla: 2.703 ± 0.516
0.394HisCys: 0.394 ± 0.143
1.183HisAsp: 1.183 ± 0.28
1.239HisGlu: 1.239 ± 0.203
0.732HisPhe: 0.732 ± 0.182
1.521HisGly: 1.521 ± 0.397
0.338HisHis: 0.338 ± 0.192
0.732HisIle: 0.732 ± 0.213
0.62HisLys: 0.62 ± 0.156
1.746HisLeu: 1.746 ± 0.345
0.394HisMet: 0.394 ± 0.142
0.394HisAsn: 0.394 ± 0.168
1.239HisPro: 1.239 ± 0.297
0.394HisGln: 0.394 ± 0.142
1.126HisArg: 1.126 ± 0.279
0.845HisSer: 0.845 ± 0.255
0.732HisThr: 0.732 ± 0.186
1.802HisVal: 1.802 ± 0.283
0.507HisTrp: 0.507 ± 0.151
0.62HisTyr: 0.62 ± 0.215
0.0HisXaa: 0.0 ± 0.0
Ile
4.28IleAla: 4.28 ± 0.429
0.169IleCys: 0.169 ± 0.105
2.253IleAsp: 2.253 ± 0.289
3.041IleGlu: 3.041 ± 0.379
0.901IlePhe: 0.901 ± 0.226
2.422IleGly: 2.422 ± 0.277
0.563IleHis: 0.563 ± 0.188
1.239IleIle: 1.239 ± 0.247
1.915IleLys: 1.915 ± 0.318
1.971IleLeu: 1.971 ± 0.347
0.676IleMet: 0.676 ± 0.195
1.295IleAsn: 1.295 ± 0.235
1.126IlePro: 1.126 ± 0.199
0.957IleGln: 0.957 ± 0.229
2.197IleArg: 2.197 ± 0.288
1.69IleSer: 1.69 ± 0.402
1.69IleThr: 1.69 ± 0.3
1.915IleVal: 1.915 ± 0.342
0.282IleTrp: 0.282 ± 0.116
0.789IleTyr: 0.789 ± 0.217
0.0IleXaa: 0.0 ± 0.0
Lys
8.054LysAla: 8.054 ± 1.636
0.338LysCys: 0.338 ± 0.148
2.422LysAsp: 2.422 ± 0.37
3.154LysGlu: 3.154 ± 0.549
1.746LysPhe: 1.746 ± 0.325
3.548LysGly: 3.548 ± 0.644
0.676LysHis: 0.676 ± 0.162
0.957LysIle: 0.957 ± 0.225
2.872LysLys: 2.872 ± 0.578
4.055LysLeu: 4.055 ± 0.589
1.295LysMet: 1.295 ± 0.267
0.957LysAsn: 0.957 ± 0.227
2.591LysPro: 2.591 ± 0.424
1.69LysGln: 1.69 ± 0.316
2.929LysArg: 2.929 ± 0.497
2.028LysSer: 2.028 ± 0.438
2.422LysThr: 2.422 ± 0.379
2.929LysVal: 2.929 ± 0.324
0.732LysTrp: 0.732 ± 0.199
1.408LysTyr: 1.408 ± 0.316
0.0LysXaa: 0.0 ± 0.0
Leu
8.843LeuAla: 8.843 ± 0.659
0.676LeuCys: 0.676 ± 0.219
6.139LeuAsp: 6.139 ± 0.488
5.407LeuGlu: 5.407 ± 0.563
2.478LeuPhe: 2.478 ± 0.315
7.153LeuGly: 7.153 ± 0.5
1.464LeuHis: 1.464 ± 0.293
2.14LeuIle: 2.14 ± 0.342
3.323LeuLys: 3.323 ± 0.515
7.209LeuLeu: 7.209 ± 0.648
2.309LeuMet: 2.309 ± 0.46
2.197LeuAsn: 2.197 ± 0.296
5.745LeuPro: 5.745 ± 0.554
3.154LeuGln: 3.154 ± 0.484
7.153LeuArg: 7.153 ± 0.653
4.844LeuSer: 4.844 ± 0.483
5.351LeuThr: 5.351 ± 0.592
7.209LeuVal: 7.209 ± 0.744
1.239LeuTrp: 1.239 ± 0.248
2.197LeuTyr: 2.197 ± 0.349
0.0LeuXaa: 0.0 ± 0.0
Met
3.323MetAla: 3.323 ± 0.355
0.225MetCys: 0.225 ± 0.146
1.746MetAsp: 1.746 ± 0.304
1.07MetGlu: 1.07 ± 0.269
0.676MetPhe: 0.676 ± 0.171
2.028MetGly: 2.028 ± 0.405
0.338MetHis: 0.338 ± 0.117
0.957MetIle: 0.957 ± 0.189
0.957MetLys: 0.957 ± 0.23
2.084MetLeu: 2.084 ± 0.391
0.394MetMet: 0.394 ± 0.156
0.732MetAsn: 0.732 ± 0.159
1.521MetPro: 1.521 ± 0.325
1.183MetGln: 1.183 ± 0.337
1.802MetArg: 1.802 ± 0.357
1.521MetSer: 1.521 ± 0.285
2.591MetThr: 2.591 ± 0.43
1.408MetVal: 1.408 ± 0.292
0.169MetTrp: 0.169 ± 0.091
0.338MetTyr: 0.338 ± 0.197
0.0MetXaa: 0.0 ± 0.0
Asn
2.197AsnAla: 2.197 ± 0.298
0.563AsnCys: 0.563 ± 0.141
1.239AsnAsp: 1.239 ± 0.238
1.802AsnGlu: 1.802 ± 0.268
0.732AsnPhe: 0.732 ± 0.179
2.816AsnGly: 2.816 ± 0.402
0.563AsnHis: 0.563 ± 0.151
0.957AsnIle: 0.957 ± 0.194
1.295AsnLys: 1.295 ± 0.325
2.309AsnLeu: 2.309 ± 0.288
0.676AsnMet: 0.676 ± 0.207
1.126AsnAsn: 1.126 ± 0.284
2.422AsnPro: 2.422 ± 0.418
0.676AsnGln: 0.676 ± 0.192
1.464AsnArg: 1.464 ± 0.244
1.521AsnSer: 1.521 ± 0.326
1.633AsnThr: 1.633 ± 0.412
2.14AsnVal: 2.14 ± 0.357
0.732AsnTrp: 0.732 ± 0.193
0.901AsnTyr: 0.901 ± 0.217
0.0AsnXaa: 0.0 ± 0.0
Pro
5.745ProAla: 5.745 ± 0.771
0.732ProCys: 0.732 ± 0.224
3.436ProAsp: 3.436 ± 0.412
3.661ProGlu: 3.661 ± 0.572
1.859ProPhe: 1.859 ± 0.263
4.224ProGly: 4.224 ± 0.618
1.014ProHis: 1.014 ± 0.27
2.028ProIle: 2.028 ± 0.282
1.746ProLys: 1.746 ± 0.33
3.267ProLeu: 3.267 ± 0.464
0.845ProMet: 0.845 ± 0.219
1.352ProAsn: 1.352 ± 0.26
2.76ProPro: 2.76 ± 0.441
1.07ProGln: 1.07 ± 0.287
3.83ProArg: 3.83 ± 0.547
3.492ProSer: 3.492 ± 0.371
4.224ProThr: 4.224 ± 0.518
3.605ProVal: 3.605 ± 0.51
0.676ProTrp: 0.676 ± 0.197
1.915ProTyr: 1.915 ± 0.343
0.0ProXaa: 0.0 ± 0.0
Gln
4.168GlnAla: 4.168 ± 0.665
0.282GlnCys: 0.282 ± 0.135
1.239GlnAsp: 1.239 ± 0.261
1.915GlnGlu: 1.915 ± 0.326
1.126GlnPhe: 1.126 ± 0.206
2.366GlnGly: 2.366 ± 0.377
0.563GlnHis: 0.563 ± 0.229
1.464GlnIle: 1.464 ± 0.341
1.183GlnLys: 1.183 ± 0.256
3.21GlnLeu: 3.21 ± 0.485
0.957GlnMet: 0.957 ± 0.229
1.014GlnAsn: 1.014 ± 0.273
2.084GlnPro: 2.084 ± 0.327
1.915GlnGln: 1.915 ± 0.481
2.872GlnArg: 2.872 ± 0.555
1.802GlnSer: 1.802 ± 0.383
1.69GlnThr: 1.69 ± 0.322
2.872GlnVal: 2.872 ± 0.357
0.732GlnTrp: 0.732 ± 0.238
0.901GlnTyr: 0.901 ± 0.256
0.0GlnXaa: 0.0 ± 0.0
Arg
7.716ArgAla: 7.716 ± 0.649
0.789ArgCys: 0.789 ± 0.231
4.618ArgAsp: 4.618 ± 0.527
5.914ArgGlu: 5.914 ± 0.612
2.366ArgPhe: 2.366 ± 0.34
5.689ArgGly: 5.689 ± 0.568
1.633ArgHis: 1.633 ± 0.357
2.647ArgIle: 2.647 ± 0.367
2.647ArgLys: 2.647 ± 0.297
6.252ArgLeu: 6.252 ± 0.706
1.859ArgMet: 1.859 ± 0.314
2.028ArgAsn: 2.028 ± 0.287
3.661ArgPro: 3.661 ± 0.498
2.76ArgGln: 2.76 ± 0.418
5.182ArgArg: 5.182 ± 0.65
4.675ArgSer: 4.675 ± 0.574
2.647ArgThr: 2.647 ± 0.429
4.844ArgVal: 4.844 ± 0.475
2.197ArgTrp: 2.197 ± 0.314
1.352ArgTyr: 1.352 ± 0.333
0.0ArgXaa: 0.0 ± 0.0
Ser
5.745SerAla: 5.745 ± 0.669
0.901SerCys: 0.901 ± 0.237
3.267SerAsp: 3.267 ± 0.373
2.816SerGlu: 2.816 ± 0.36
2.703SerPhe: 2.703 ± 0.501
5.632SerGly: 5.632 ± 0.666
1.239SerHis: 1.239 ± 0.252
2.366SerIle: 2.366 ± 0.363
2.422SerLys: 2.422 ± 0.392
4.675SerLeu: 4.675 ± 0.521
0.957SerMet: 0.957 ± 0.227
1.183SerAsn: 1.183 ± 0.307
2.534SerPro: 2.534 ± 0.418
1.746SerGln: 1.746 ± 0.349
3.492SerArg: 3.492 ± 0.552
3.661SerSer: 3.661 ± 0.588
4.112SerThr: 4.112 ± 0.696
4.449SerVal: 4.449 ± 0.611
1.014SerTrp: 1.014 ± 0.22
2.028SerTyr: 2.028 ± 0.373
0.0SerXaa: 0.0 ± 0.0
Thr
5.182ThrAla: 5.182 ± 0.535
0.507ThrCys: 0.507 ± 0.156
3.661ThrAsp: 3.661 ± 0.474
3.21ThrGlu: 3.21 ± 0.372
1.464ThrPhe: 1.464 ± 0.311
5.182ThrGly: 5.182 ± 0.641
0.507ThrHis: 0.507 ± 0.161
2.591ThrIle: 2.591 ± 0.302
2.084ThrLys: 2.084 ± 0.309
4.731ThrLeu: 4.731 ± 0.443
1.464ThrMet: 1.464 ± 0.269
1.971ThrAsn: 1.971 ± 0.357
3.605ThrPro: 3.605 ± 0.422
1.577ThrGln: 1.577 ± 0.269
2.703ThrArg: 2.703 ± 0.337
4.28ThrSer: 4.28 ± 0.661
3.605ThrThr: 3.605 ± 0.505
4.9ThrVal: 4.9 ± 0.568
1.239ThrTrp: 1.239 ± 0.238
1.239ThrTyr: 1.239 ± 0.221
0.0ThrXaa: 0.0 ± 0.0
Val
8.843ValAla: 8.843 ± 0.845
0.507ValCys: 0.507 ± 0.184
5.632ValAsp: 5.632 ± 0.549
5.689ValGlu: 5.689 ± 0.562
1.802ValPhe: 1.802 ± 0.293
5.351ValGly: 5.351 ± 0.638
1.802ValHis: 1.802 ± 0.286
2.366ValIle: 2.366 ± 0.381
3.661ValLys: 3.661 ± 0.487
6.815ValLeu: 6.815 ± 0.617
1.521ValMet: 1.521 ± 0.329
2.14ValAsn: 2.14 ± 0.355
3.83ValPro: 3.83 ± 0.436
2.591ValGln: 2.591 ± 0.325
5.914ValArg: 5.914 ± 0.617
4.224ValSer: 4.224 ± 0.482
4.28ValThr: 4.28 ± 0.54
6.026ValVal: 6.026 ± 0.469
1.577ValTrp: 1.577 ± 0.316
1.464ValTyr: 1.464 ± 0.315
0.0ValXaa: 0.0 ± 0.0
Trp
1.802TrpAla: 1.802 ± 0.344
0.225TrpCys: 0.225 ± 0.109
1.802TrpAsp: 1.802 ± 0.361
1.802TrpGlu: 1.802 ± 0.238
0.732TrpPhe: 0.732 ± 0.225
1.014TrpGly: 1.014 ± 0.224
0.507TrpHis: 0.507 ± 0.203
0.451TrpIle: 0.451 ± 0.179
0.676TrpLys: 0.676 ± 0.252
1.408TrpLeu: 1.408 ± 0.373
0.338TrpMet: 0.338 ± 0.14
0.451TrpAsn: 0.451 ± 0.143
0.676TrpPro: 0.676 ± 0.177
0.789TrpGln: 0.789 ± 0.27
1.69TrpArg: 1.69 ± 0.436
1.295TrpSer: 1.295 ± 0.255
1.183TrpThr: 1.183 ± 0.259
1.295TrpVal: 1.295 ± 0.261
0.169TrpTrp: 0.169 ± 0.101
0.957TrpTyr: 0.957 ± 0.166
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.929TyrAla: 2.929 ± 0.428
0.507TyrCys: 0.507 ± 0.171
1.633TyrAsp: 1.633 ± 0.295
1.746TyrGlu: 1.746 ± 0.305
0.507TyrPhe: 0.507 ± 0.185
2.422TyrGly: 2.422 ± 0.366
0.789TyrHis: 0.789 ± 0.208
0.394TyrIle: 0.394 ± 0.165
1.07TyrLys: 1.07 ± 0.245
2.197TyrLeu: 2.197 ± 0.361
0.789TyrMet: 0.789 ± 0.237
0.901TyrAsn: 0.901 ± 0.243
1.464TyrPro: 1.464 ± 0.275
1.183TyrGln: 1.183 ± 0.266
2.647TyrArg: 2.647 ± 0.41
1.521TyrSer: 1.521 ± 0.269
1.408TyrThr: 1.408 ± 0.335
1.971TyrVal: 1.971 ± 0.331
0.563TyrTrp: 0.563 ± 0.234
1.07TyrTyr: 1.07 ± 0.282
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 76 proteins (17756 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski