Alteromonas phage vB_AmaP_AD45-P1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 120 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4W0W4|R4W0W4_9CAUD Uncharacterized protein OS=Alteromonas phage vB_AmaP_AD45-P1 OX=1300004 GN=AD45P1_00185 PE=4 SV=1
MM1 pKa = 7.28NLVEE5 pKa = 4.79TIVPVIKK12 pKa = 10.43VAEE15 pKa = 4.14FHH17 pKa = 7.21AITGYY22 pKa = 10.38QIEE25 pKa = 4.88TISAMFDD32 pKa = 3.32EE33 pKa = 5.4YY34 pKa = 10.77VTEE37 pKa = 4.1LDD39 pKa = 3.23APIRR43 pKa = 11.84HH44 pKa = 6.88DD45 pKa = 4.27SGDD48 pKa = 3.23IDD50 pKa = 4.52AEE52 pKa = 4.39MVTEE56 pKa = 5.73LFDD59 pKa = 4.89QMLTTYY65 pKa = 10.67RR66 pKa = 11.84EE67 pKa = 4.22AQPCSS72 pKa = 3.36

Molecular weight: 8.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4W0X9|R4W0X9_9CAUD Uncharacterized protein OS=Alteromonas phage vB_AmaP_AD45-P1 OX=1300004 GN=AD45P1_00310 PE=4 SV=1
MM1 pKa = 7.25KK2 pKa = 10.43VDD4 pKa = 3.89WKK6 pKa = 10.82QVASSPGYY14 pKa = 10.12KK15 pKa = 9.65SLKK18 pKa = 9.03AALVRR23 pKa = 11.84DD24 pKa = 3.28IGGNRR29 pKa = 11.84RR30 pKa = 11.84NMSGRR35 pKa = 11.84SKK37 pKa = 10.97EE38 pKa = 3.73EE39 pKa = 3.44MYY41 pKa = 11.32ARR43 pKa = 11.84FNWIIARR50 pKa = 11.84AQHH53 pKa = 5.66YY54 pKa = 10.27AHH56 pKa = 6.2VQNRR60 pKa = 11.84PLSSILNEE68 pKa = 4.11WEE70 pKa = 4.59DD71 pKa = 4.49KK72 pKa = 10.73RR73 pKa = 11.84SSWWFGHH80 pKa = 5.36YY81 pKa = 10.32AEE83 pKa = 5.22SSHH86 pKa = 6.83PKK88 pKa = 10.17LGSGKK93 pKa = 9.07PKK95 pKa = 10.17NVKK98 pKa = 8.2PQSMRR103 pKa = 11.84NYY105 pKa = 10.02YY106 pKa = 8.53KK107 pKa = 9.78TDD109 pKa = 2.51PWLRR113 pKa = 11.84RR114 pKa = 11.84KK115 pKa = 9.7SPKK118 pKa = 9.94EE119 pKa = 3.35RR120 pKa = 11.84FKK122 pKa = 10.98QIRR125 pKa = 11.84NTKK128 pKa = 7.37TRR130 pKa = 11.84EE131 pKa = 3.72AKK133 pKa = 10.09FNRR136 pKa = 11.84EE137 pKa = 3.37HH138 pKa = 7.44RR139 pKa = 11.84LGKK142 pKa = 9.73KK143 pKa = 9.46PRR145 pKa = 11.84WSPDD149 pKa = 2.59RR150 pKa = 11.84KK151 pKa = 10.19RR152 pKa = 11.84INATYY157 pKa = 10.41RR158 pKa = 11.84RR159 pKa = 11.84IKK161 pKa = 10.07RR162 pKa = 11.84QEE164 pKa = 3.7NLL166 pKa = 3.18

Molecular weight: 19.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

120

0

120

27410

60

2201

228.4

25.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.282 ± 0.221

0.817 ± 0.097

6.611 ± 0.229

7.205 ± 0.392

3.597 ± 0.16

6.06 ± 0.439

1.926 ± 0.141

5.688 ± 0.156

7.107 ± 0.368

7.935 ± 0.302

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.298 ± 0.169

5.815 ± 0.31

3.408 ± 0.175

3.594 ± 0.195

4.528 ± 0.157

6.727 ± 0.325

6.29 ± 0.298

7.019 ± 0.247

1.175 ± 0.096

3.918 ± 0.207
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski