Thiorhodococcus minor

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Chromatiaceae; Thiorhodococcus

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5194 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M0JXT8|A0A6M0JXT8_9GAMM Light-harvesting protein OS=Thiorhodococcus minor OX=57489 GN=G3446_06015 PE=4 SV=1
MM1 pKa = 7.75DD2 pKa = 5.01RR3 pKa = 11.84SRR5 pKa = 11.84FLSRR9 pKa = 11.84VGGGCLLAIGLAGAATPATAEE30 pKa = 3.83ISFSNVTGSSGITDD44 pKa = 3.38TGEE47 pKa = 4.03TFSTGWGDD55 pKa = 3.41FAGTGFPGIWLNKK68 pKa = 8.32HH69 pKa = 5.54QYY71 pKa = 7.86TPTRR75 pKa = 11.84IFYY78 pKa = 9.68NLSGSGFTEE87 pKa = 4.17VADD90 pKa = 3.92DD91 pKa = 3.67VLKK94 pKa = 11.23NEE96 pKa = 4.64EE97 pKa = 4.75GVPSPQTDD105 pKa = 3.86FNIPRR110 pKa = 11.84AEE112 pKa = 4.08TGPGEE117 pKa = 4.2FSYY120 pKa = 10.8FADD123 pKa = 3.37THH125 pKa = 6.05GQTSADD131 pKa = 3.45WDD133 pKa = 3.72NDD135 pKa = 2.85GDD137 pKa = 4.02QDD139 pKa = 4.02VLEE142 pKa = 4.38VTGAGYY148 pKa = 10.16IFPLWEE154 pKa = 4.05NDD156 pKa = 3.08GLGNFEE162 pKa = 4.09YY163 pKa = 10.61RR164 pKa = 11.84GDD166 pKa = 4.03EE167 pKa = 4.22LGFAYY172 pKa = 9.89PLTWEE177 pKa = 4.0RR178 pKa = 11.84RR179 pKa = 11.84EE180 pKa = 3.87RR181 pKa = 11.84PPIGGRR187 pKa = 11.84DD188 pKa = 3.54SLFFDD193 pKa = 4.16YY194 pKa = 11.05DD195 pKa = 3.55RR196 pKa = 11.84DD197 pKa = 3.56GWLDD201 pKa = 3.44AMVMARR207 pKa = 11.84EE208 pKa = 4.2NYY210 pKa = 8.86SAYY213 pKa = 8.47RR214 pKa = 11.84TPTAIFRR221 pKa = 11.84QEE223 pKa = 4.09RR224 pKa = 11.84IGGEE228 pKa = 4.1SFFTLDD234 pKa = 3.13EE235 pKa = 4.45STGVVPDD242 pKa = 4.12EE243 pKa = 4.46GTGPFPGTACEE254 pKa = 4.19FGAMAEE260 pKa = 4.06LSGDD264 pKa = 3.59SVLDD268 pKa = 4.17VICSDD273 pKa = 3.91SQISAIWDD281 pKa = 3.43VSQLPFADD289 pKa = 3.75LRR291 pKa = 11.84PVVGDD296 pKa = 3.92EE297 pKa = 3.72IFNRR301 pKa = 11.84YY302 pKa = 8.14PADD305 pKa = 3.53LAIGDD310 pKa = 4.31FNNDD314 pKa = 2.62LRR316 pKa = 11.84TDD318 pKa = 3.28IMAPTFAGADD328 pKa = 3.5YY329 pKa = 10.74VRR331 pKa = 11.84NLVGVVDD338 pKa = 4.62GQTLHH343 pKa = 6.07GWFNTSVPSNSGFNFSATGNVTFEE367 pKa = 4.18FDD369 pKa = 2.85WWTEE373 pKa = 3.84TPNIFIGADD382 pKa = 3.31GSNPDD387 pKa = 4.16LATNLPTMPFQEE399 pKa = 5.11LGHH402 pKa = 6.88GDD404 pKa = 3.78PNHH407 pKa = 5.9VVVTLSPTEE416 pKa = 4.08AQGLAPIFVPEE427 pKa = 5.18DD428 pKa = 3.46NDD430 pKa = 3.65GDD432 pKa = 4.83PITGVFIGYY441 pKa = 9.83VDD443 pKa = 3.81GEE445 pKa = 4.1WRR447 pKa = 11.84VRR449 pKa = 11.84FSGIEE454 pKa = 3.56RR455 pKa = 11.84WNVGMVITAQSGISGLTSVGGTKK478 pKa = 9.57IGQDD482 pKa = 3.07TGGNALLFLQSEE494 pKa = 4.32DD495 pKa = 3.76HH496 pKa = 6.62EE497 pKa = 4.96LVFSGGNVQAVNRR510 pKa = 11.84SCTAAAAADD519 pKa = 4.06FDD521 pKa = 4.55NDD523 pKa = 2.84MDD525 pKa = 5.34LDD527 pKa = 4.03VFFACSGQLSNLPDD541 pKa = 3.09IVYY544 pKa = 9.9EE545 pKa = 4.07NQGNGTFVALPAAGGAEE562 pKa = 3.96GQMPLGRR569 pKa = 11.84ADD571 pKa = 3.48SVMVADD577 pKa = 4.02YY578 pKa = 11.55DD579 pKa = 3.9EE580 pKa = 4.71DD581 pKa = 4.0CRR583 pKa = 11.84MDD585 pKa = 3.61VYY587 pKa = 10.09VTNGHH592 pKa = 6.1HH593 pKa = 6.29FRR595 pKa = 11.84PSSYY599 pKa = 11.24AGIHH603 pKa = 4.18QLFRR607 pKa = 11.84NTTSDD612 pKa = 3.58GNHH615 pKa = 7.35CIQIDD620 pKa = 4.01LEE622 pKa = 4.64GTVSNRR628 pKa = 11.84DD629 pKa = 3.31GIGAIVYY636 pKa = 7.54ATTPDD641 pKa = 3.19GTVQKK646 pKa = 10.36RR647 pKa = 11.84DD648 pKa = 3.55QNGGKK653 pKa = 9.2HH654 pKa = 6.14KK655 pKa = 10.44KK656 pKa = 8.6AQDD659 pKa = 3.54YY660 pKa = 10.16QRR662 pKa = 11.84VHH664 pKa = 6.8IGLGTNQSVDD674 pKa = 4.61LEE676 pKa = 4.55VHH678 pKa = 5.4WPSGIVEE685 pKa = 4.39TFANVAADD693 pKa = 3.49QVVEE697 pKa = 4.17LVEE700 pKa = 4.47GTGGTGPSYY709 pKa = 10.86SLSVDD714 pKa = 3.52DD715 pKa = 4.16PTVTEE720 pKa = 4.73GSDD723 pKa = 3.32TQALFTVNLSPAPGAGEE740 pKa = 4.2TVLVDD745 pKa = 3.38YY746 pKa = 8.5QTANGSAQAGSDD758 pKa = 3.68YY759 pKa = 10.85TSTSGQLSFGEE770 pKa = 4.64GDD772 pKa = 3.21SSLTVSVPILDD783 pKa = 5.22DD784 pKa = 3.55GTAEE788 pKa = 4.05PNEE791 pKa = 4.39TFTLQASSSEE801 pKa = 4.0ASPASGTATIQDD813 pKa = 4.0DD814 pKa = 4.48DD815 pKa = 5.15GSGGDD820 pKa = 4.22PTCGMPTFDD829 pKa = 3.99PAVDD833 pKa = 3.07RR834 pKa = 11.84GVYY837 pKa = 9.53LWNDD841 pKa = 3.25CGTNDD846 pKa = 2.56WHH848 pKa = 8.04LRR850 pKa = 11.84ITGGGSGQYY859 pKa = 10.34LNHH862 pKa = 7.12LGTLVADD869 pKa = 4.01APFVSVDD876 pKa = 3.45GFSVEE881 pKa = 5.15ANDD884 pKa = 4.01TLDD887 pKa = 3.53VAGSQIDD894 pKa = 3.33FGLIVGGVYY903 pKa = 10.46EE904 pKa = 4.88DD905 pKa = 5.46GIDD908 pKa = 3.54FTLAEE913 pKa = 4.42GASVCFTLTSPSGLAVFGGVDD934 pKa = 4.11ADD936 pKa = 3.91PVSLPVSMPGFGDD949 pKa = 3.64CGGGGGDD956 pKa = 4.26PTDD959 pKa = 4.24PTCGMPTFDD968 pKa = 4.21PASDD972 pKa = 3.05RR973 pKa = 11.84GVYY976 pKa = 8.89LWKK979 pKa = 10.69DD980 pKa = 3.27CGTDD984 pKa = 3.29DD985 pKa = 2.8WHH987 pKa = 8.33LRR989 pKa = 11.84ITGGSSGQYY998 pKa = 9.87LNHH1001 pKa = 6.94IGQLVADD1008 pKa = 4.53APFLDD1013 pKa = 3.76VSGVSIEE1020 pKa = 4.62GNDD1023 pKa = 3.68TLDD1026 pKa = 3.31IGASQVDD1033 pKa = 4.13FGLIVGGIYY1042 pKa = 10.25EE1043 pKa = 4.64DD1044 pKa = 5.54GIDD1047 pKa = 3.58FTLAEE1052 pKa = 4.42GASVCFSLTSPSGLSVFGGVDD1073 pKa = 3.81ADD1075 pKa = 3.96PVTLPVSMPGFGACDD1090 pKa = 3.54GGGGDD1095 pKa = 4.51PTDD1098 pKa = 4.23PTCGMPSFDD1107 pKa = 3.85PASDD1111 pKa = 3.07RR1112 pKa = 11.84GAFLWKK1118 pKa = 10.47DD1119 pKa = 3.33CGTDD1123 pKa = 3.29DD1124 pKa = 2.8WHH1126 pKa = 8.39LRR1128 pKa = 11.84ITGGGNGQYY1137 pKa = 9.88VGHH1140 pKa = 6.53VGSLVADD1147 pKa = 3.83APFVSVDD1154 pKa = 3.42GVSIEE1159 pKa = 4.65ANDD1162 pKa = 3.93TFDD1165 pKa = 3.17ITASQVDD1172 pKa = 4.11FGLIVGGIYY1181 pKa = 10.25EE1182 pKa = 4.64DD1183 pKa = 5.49GIDD1186 pKa = 3.73FTLSQGSSACFTLDD1200 pKa = 2.89SPTDD1204 pKa = 3.37VSVFGGVDD1212 pKa = 3.36GTDD1215 pKa = 2.88VGSPVGVPGFGACAVPP1231 pKa = 4.3

Molecular weight: 128.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M0K148|A0A6M0K148_9GAMM Peptide methionine sulfoxide reductase MsrA OS=Thiorhodococcus minor OX=57489 GN=msrA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LKK11 pKa = 10.48RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 6.41GFRR19 pKa = 11.84ARR21 pKa = 11.84CATRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 9.43VIAARR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.62GRR39 pKa = 11.84ARR41 pKa = 11.84LAPP44 pKa = 4.03

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5194

0

5194

1678515

23

3182

323.2

35.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.569 ± 0.039

1.041 ± 0.014

5.938 ± 0.027

6.428 ± 0.033

3.309 ± 0.018

8.081 ± 0.033

2.206 ± 0.018

4.733 ± 0.025

2.781 ± 0.027

11.43 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.185 ± 0.016

2.18 ± 0.017

5.278 ± 0.025

3.807 ± 0.022

7.914 ± 0.042

5.532 ± 0.026

4.859 ± 0.025

6.909 ± 0.033

1.451 ± 0.016

2.37 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski