Tetragenococcus muriaticus 3MR10-3

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Tetragenococcus; Tetragenococcus muriaticus

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2262 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A091C807|A0A091C807_9ENTE Di/tripeptide permease OS=Tetragenococcus muriaticus 3MR10-3 OX=1302648 GN=TMU3MR103_0517 PE=3 SV=1
MM1 pKa = 7.59APYY4 pKa = 10.44DD5 pKa = 3.66ATEE8 pKa = 4.05EE9 pKa = 4.19TGSVEE14 pKa = 4.24TQNDD18 pKa = 3.36EE19 pKa = 4.77TVTEE23 pKa = 4.38DD24 pKa = 3.69ANTEE28 pKa = 4.07PEE30 pKa = 4.24VNRR33 pKa = 11.84SYY35 pKa = 11.6SEE37 pKa = 4.17TEE39 pKa = 3.76EE40 pKa = 4.07QQPSSAEE47 pKa = 4.02DD48 pKa = 3.27QTSYY52 pKa = 11.65AQGQEE57 pKa = 3.94QSQTADD63 pKa = 2.96EE64 pKa = 4.47RR65 pKa = 11.84SQLEE69 pKa = 4.04EE70 pKa = 3.75QQEE73 pKa = 4.56GEE75 pKa = 4.13EE76 pKa = 4.45APAEE80 pKa = 4.07EE81 pKa = 4.73DD82 pKa = 2.96EE83 pKa = 4.55QYY85 pKa = 11.76ANEE88 pKa = 4.03EE89 pKa = 3.95DD90 pKa = 3.86QIAEE94 pKa = 4.21EE95 pKa = 4.21EE96 pKa = 4.27AQTTEE101 pKa = 4.19EE102 pKa = 4.45EE103 pKa = 4.12EE104 pKa = 4.53TYY106 pKa = 11.06DD107 pKa = 3.19ATEE110 pKa = 3.82EE111 pKa = 4.09MEE113 pKa = 4.34EE114 pKa = 4.43LEE116 pKa = 4.96LPEE119 pKa = 6.64DD120 pKa = 4.04EE121 pKa = 5.55DD122 pKa = 3.74AQLQEE127 pKa = 4.97EE128 pKa = 4.98VGQHH132 pKa = 6.22PGDD135 pKa = 3.72NNGPAVSTISSNAPEE150 pKa = 3.88PSAFIEE156 pKa = 4.2EE157 pKa = 4.39LAGHH161 pKa = 6.87AEE163 pKa = 4.23SVAAANNLYY172 pKa = 10.26ASVMIAQAVVEE183 pKa = 4.76SGWGPSTLSQAPNNNLFGIKK203 pKa = 8.58GTYY206 pKa = 9.41NGQSVKK212 pKa = 10.14MPTQEE217 pKa = 4.42YY218 pKa = 9.67INGRR222 pKa = 11.84YY223 pKa = 6.78VTVNADD229 pKa = 3.19FRR231 pKa = 11.84KK232 pKa = 9.07YY233 pKa = 10.31PSYY236 pKa = 9.78TASFQDD242 pKa = 3.43NAALLSTNLYY252 pKa = 9.79SGAWKK257 pKa = 10.76SNTSSYY263 pKa = 11.18RR264 pKa = 11.84DD265 pKa = 3.25ATAALTGLYY274 pKa = 8.38ATAPNYY280 pKa = 8.26NTVLNGVIEE289 pKa = 4.97DD290 pKa = 4.0YY291 pKa = 11.32NLTRR295 pKa = 11.84FDD297 pKa = 4.02TGDD300 pKa = 3.14SDD302 pKa = 5.79GIIDD306 pKa = 4.13TGTGNDD312 pKa = 3.89GNSSEE317 pKa = 5.27DD318 pKa = 3.65NNNEE322 pKa = 3.95DD323 pKa = 3.77NSNNEE328 pKa = 3.89NSSSNSGSNTYY339 pKa = 7.27TVRR342 pKa = 11.84PGDD345 pKa = 4.42SVWLIANNNGISMDD359 pKa = 4.5QLRR362 pKa = 11.84SWNNIQNDD370 pKa = 3.82FVYY373 pKa = 10.07PGQEE377 pKa = 3.84LTVSQGNNSSGSNNSNNSGSSNDD400 pKa = 3.75NNNSSSSGSDD410 pKa = 2.9TYY412 pKa = 9.58TVRR415 pKa = 11.84SGDD418 pKa = 4.32SVWLIANNNGISMDD432 pKa = 4.5QLRR435 pKa = 11.84SWNNIQNDD443 pKa = 3.82FVYY446 pKa = 10.07PGQEE450 pKa = 3.84LTVSQGNSSSGSNNSNSGGSSNSGNNSGGSYY481 pKa = 7.52TVRR484 pKa = 11.84SGDD487 pKa = 4.22SVWLIANNNGISMDD501 pKa = 4.5QLRR504 pKa = 11.84SWNNIQNDD512 pKa = 3.89FVYY515 pKa = 10.17PGQTLTVNNGSSSNRR530 pKa = 11.84NTNQSNNSSNNNHH543 pKa = 6.44RR544 pKa = 11.84VTSGDD549 pKa = 4.06SLWMISQEE557 pKa = 3.88YY558 pKa = 9.92GIAISQLKK566 pKa = 10.05SINNLNSDD574 pKa = 4.39TIFIGQTLRR583 pKa = 11.84VSS585 pKa = 3.47

Molecular weight: 63.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A091BWM5|A0A091BWM5_9ENTE DNA gyrase subunit B OS=Tetragenococcus muriaticus 3MR10-3 OX=1302648 GN=gyrB PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 9.14RR10 pKa = 11.84KK11 pKa = 8.54HH12 pKa = 5.23QKK14 pKa = 8.76VHH16 pKa = 5.58GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 10.16NGRR28 pKa = 11.84HH29 pKa = 4.05VLANRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.35GRR39 pKa = 11.84KK40 pKa = 8.82KK41 pKa = 10.58LSAA44 pKa = 3.95

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2262

0

2262

565595

37

1424

250.0

28.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.871 ± 0.046

0.572 ± 0.014

5.66 ± 0.046

7.806 ± 0.067

4.575 ± 0.044

6.399 ± 0.054

1.836 ± 0.027

7.435 ± 0.05

6.564 ± 0.054

9.647 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.683 ± 0.024

4.85 ± 0.039

3.371 ± 0.028

4.775 ± 0.051

3.923 ± 0.038

5.974 ± 0.041

5.75 ± 0.032

6.718 ± 0.041

0.914 ± 0.019

3.676 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski