Amino acid dipepetide frequency for Pseudoalteromonas phage vB_PspS-H40/1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.33AlaAla: 7.33 ± 1.089
0.705AlaCys: 0.705 ± 0.208
3.383AlaAsp: 3.383 ± 0.464
5.568AlaGlu: 5.568 ± 0.834
3.031AlaPhe: 3.031 ± 0.496
5.145AlaGly: 5.145 ± 0.607
1.48AlaHis: 1.48 ± 0.336
5.639AlaIle: 5.639 ± 0.651
5.216AlaLys: 5.216 ± 0.724
6.907AlaLeu: 6.907 ± 0.828
2.678AlaMet: 2.678 ± 0.361
5.498AlaAsn: 5.498 ± 0.713
2.255AlaPro: 2.255 ± 0.445
3.806AlaGln: 3.806 ± 0.592
2.819AlaArg: 2.819 ± 0.466
4.863AlaSer: 4.863 ± 0.811
5.216AlaThr: 5.216 ± 0.686
4.652AlaVal: 4.652 ± 0.5
0.846AlaTrp: 0.846 ± 0.218
2.608AlaTyr: 2.608 ± 0.442
0.0AlaXaa: 0.0 ± 0.0
Cys
0.916CysAla: 0.916 ± 0.306
0.211CysCys: 0.211 ± 0.127
0.634CysAsp: 0.634 ± 0.24
0.987CysGlu: 0.987 ± 0.284
0.352CysPhe: 0.352 ± 0.154
0.846CysGly: 0.846 ± 0.197
0.352CysHis: 0.352 ± 0.147
0.634CysIle: 0.634 ± 0.21
1.128CysLys: 1.128 ± 0.276
1.269CysLeu: 1.269 ± 0.394
0.211CysMet: 0.211 ± 0.113
0.846CysAsn: 0.846 ± 0.215
0.423CysPro: 0.423 ± 0.269
0.493CysGln: 0.493 ± 0.19
0.423CysArg: 0.423 ± 0.183
1.128CysSer: 1.128 ± 0.347
0.846CysThr: 0.846 ± 0.222
0.987CysVal: 0.987 ± 0.289
0.282CysTrp: 0.282 ± 0.202
0.423CysTyr: 0.423 ± 0.175
0.0CysXaa: 0.0 ± 0.0
Asp
4.299AspAla: 4.299 ± 0.73
1.41AspCys: 1.41 ± 0.362
4.158AspAsp: 4.158 ± 0.578
4.581AspGlu: 4.581 ± 0.569
3.454AspPhe: 3.454 ± 0.431
5.357AspGly: 5.357 ± 0.6
0.705AspHis: 0.705 ± 0.304
4.934AspIle: 4.934 ± 0.64
3.947AspLys: 3.947 ± 0.581
4.44AspLeu: 4.44 ± 0.526
1.551AspMet: 1.551 ± 0.39
3.383AspAsn: 3.383 ± 0.585
2.114AspPro: 2.114 ± 0.47
1.48AspGln: 1.48 ± 0.334
1.762AspArg: 1.762 ± 0.339
5.286AspSer: 5.286 ± 0.508
3.242AspThr: 3.242 ± 0.483
3.736AspVal: 3.736 ± 0.478
0.916AspTrp: 0.916 ± 0.266
2.819AspTyr: 2.819 ± 0.374
0.0AspXaa: 0.0 ± 0.0
Glu
5.075GluAla: 5.075 ± 0.78
0.846GluCys: 0.846 ± 0.23
2.608GluAsp: 2.608 ± 0.449
2.749GluGlu: 2.749 ± 0.44
2.89GluPhe: 2.89 ± 0.419
3.736GluGly: 3.736 ± 0.627
1.339GluHis: 1.339 ± 0.276
5.145GluIle: 5.145 ± 0.633
4.793GluLys: 4.793 ± 0.811
7.189GluLeu: 7.189 ± 0.716
1.903GluMet: 1.903 ± 0.347
2.819GluAsn: 2.819 ± 0.415
2.044GluPro: 2.044 ± 0.344
2.537GluGln: 2.537 ± 0.379
3.524GluArg: 3.524 ± 0.443
4.511GluSer: 4.511 ± 0.697
2.96GluThr: 2.96 ± 0.448
4.158GluVal: 4.158 ± 0.45
0.987GluTrp: 0.987 ± 0.262
3.595GluTyr: 3.595 ± 0.45
0.0GluXaa: 0.0 ± 0.0
Phe
3.172PheAla: 3.172 ± 0.423
0.775PheCys: 0.775 ± 0.235
3.665PheAsp: 3.665 ± 0.61
2.396PheGlu: 2.396 ± 0.361
1.128PhePhe: 1.128 ± 0.275
2.114PheGly: 2.114 ± 0.408
0.493PheHis: 0.493 ± 0.193
2.749PheIle: 2.749 ± 0.486
3.313PheLys: 3.313 ± 0.503
2.255PheLeu: 2.255 ± 0.34
0.705PheMet: 0.705 ± 0.261
3.313PheAsn: 3.313 ± 0.456
1.269PhePro: 1.269 ± 0.374
0.846PheGln: 0.846 ± 0.223
1.551PheArg: 1.551 ± 0.375
2.819PheSer: 2.819 ± 0.388
3.454PheThr: 3.454 ± 0.511
2.89PheVal: 2.89 ± 0.379
0.141PheTrp: 0.141 ± 0.096
1.269PheTyr: 1.269 ± 0.318
0.0PheXaa: 0.0 ± 0.0
Gly
5.498GlyAla: 5.498 ± 0.921
0.775GlyCys: 0.775 ± 0.22
3.806GlyAsp: 3.806 ± 0.444
4.581GlyGlu: 4.581 ± 0.564
3.665GlyPhe: 3.665 ± 0.521
6.202GlyGly: 6.202 ± 0.663
0.775GlyHis: 0.775 ± 0.298
4.37GlyIle: 4.37 ± 0.49
5.075GlyLys: 5.075 ± 0.527
5.004GlyLeu: 5.004 ± 0.499
1.692GlyMet: 1.692 ± 0.355
4.017GlyAsn: 4.017 ± 0.476
0.846GlyPro: 0.846 ± 0.21
2.396GlyGln: 2.396 ± 0.484
3.242GlyArg: 3.242 ± 0.477
4.722GlySer: 4.722 ± 0.814
2.89GlyThr: 2.89 ± 0.598
4.934GlyVal: 4.934 ± 0.747
1.128GlyTrp: 1.128 ± 0.294
2.396GlyTyr: 2.396 ± 0.447
0.0GlyXaa: 0.0 ± 0.0
His
0.775HisAla: 0.775 ± 0.207
0.352HisCys: 0.352 ± 0.167
0.775HisAsp: 0.775 ± 0.242
0.634HisGlu: 0.634 ± 0.177
1.057HisPhe: 1.057 ± 0.286
1.41HisGly: 1.41 ± 0.305
0.423HisHis: 0.423 ± 0.191
1.057HisIle: 1.057 ± 0.351
1.692HisLys: 1.692 ± 0.396
1.269HisLeu: 1.269 ± 0.312
0.564HisMet: 0.564 ± 0.162
0.634HisAsn: 0.634 ± 0.203
0.846HisPro: 0.846 ± 0.25
0.564HisGln: 0.564 ± 0.182
0.634HisArg: 0.634 ± 0.203
1.198HisSer: 1.198 ± 0.286
0.282HisThr: 0.282 ± 0.136
1.621HisVal: 1.621 ± 0.354
0.211HisTrp: 0.211 ± 0.118
0.634HisTyr: 0.634 ± 0.187
0.0HisXaa: 0.0 ± 0.0
Ile
4.934IleAla: 4.934 ± 0.548
0.916IleCys: 0.916 ± 0.231
6.132IleAsp: 6.132 ± 0.66
6.273IleGlu: 6.273 ± 0.796
2.608IlePhe: 2.608 ± 0.351
5.498IleGly: 5.498 ± 0.706
0.846IleHis: 0.846 ± 0.313
3.172IleIle: 3.172 ± 0.492
5.709IleLys: 5.709 ± 0.607
3.806IleLeu: 3.806 ± 0.58
1.48IleMet: 1.48 ± 0.312
4.44IleAsn: 4.44 ± 0.554
2.114IlePro: 2.114 ± 0.37
1.48IleGln: 1.48 ± 0.299
2.608IleArg: 2.608 ± 0.452
4.229IleSer: 4.229 ± 0.656
4.652IleThr: 4.652 ± 0.643
3.454IleVal: 3.454 ± 0.413
0.705IleTrp: 0.705 ± 0.248
1.903IleTyr: 1.903 ± 0.393
0.0IleXaa: 0.0 ± 0.0
Lys
6.625LysAla: 6.625 ± 0.928
0.775LysCys: 0.775 ± 0.253
3.242LysAsp: 3.242 ± 0.57
4.581LysGlu: 4.581 ± 0.691
2.114LysPhe: 2.114 ± 0.4
4.158LysGly: 4.158 ± 0.51
1.903LysHis: 1.903 ± 0.39
4.722LysIle: 4.722 ± 0.541
4.934LysLys: 4.934 ± 0.734
6.132LysLeu: 6.132 ± 0.92
2.044LysMet: 2.044 ± 0.333
3.101LysAsn: 3.101 ± 0.5
3.665LysPro: 3.665 ± 0.518
2.89LysGln: 2.89 ± 0.524
3.101LysArg: 3.101 ± 0.414
4.44LysSer: 4.44 ± 0.47
3.806LysThr: 3.806 ± 0.613
3.524LysVal: 3.524 ± 0.512
1.41LysTrp: 1.41 ± 0.324
3.524LysTyr: 3.524 ± 0.535
0.0LysXaa: 0.0 ± 0.0
Leu
6.766LeuAla: 6.766 ± 0.865
1.057LeuCys: 1.057 ± 0.317
5.145LeuAsp: 5.145 ± 0.502
4.44LeuGlu: 4.44 ± 0.482
2.467LeuPhe: 2.467 ± 0.428
4.863LeuGly: 4.863 ± 0.506
1.621LeuHis: 1.621 ± 0.315
5.991LeuIle: 5.991 ± 0.736
4.722LeuLys: 4.722 ± 0.614
5.004LeuLeu: 5.004 ± 0.618
2.044LeuMet: 2.044 ± 0.339
4.088LeuAsn: 4.088 ± 0.456
2.326LeuPro: 2.326 ± 0.332
3.313LeuGln: 3.313 ± 0.505
2.89LeuArg: 2.89 ± 0.516
4.793LeuSer: 4.793 ± 0.519
5.639LeuThr: 5.639 ± 0.924
4.511LeuVal: 4.511 ± 0.641
0.916LeuTrp: 0.916 ± 0.248
2.044LeuTyr: 2.044 ± 0.449
0.0LeuXaa: 0.0 ± 0.0
Met
2.819MetAla: 2.819 ± 0.469
0.141MetCys: 0.141 ± 0.089
1.198MetAsp: 1.198 ± 0.315
1.269MetGlu: 1.269 ± 0.29
0.564MetPhe: 0.564 ± 0.163
1.269MetGly: 1.269 ± 0.387
0.282MetHis: 0.282 ± 0.127
1.41MetIle: 1.41 ± 0.382
2.255MetLys: 2.255 ± 0.475
1.762MetLeu: 1.762 ± 0.342
0.493MetMet: 0.493 ± 0.19
1.198MetAsn: 1.198 ± 0.315
0.916MetPro: 0.916 ± 0.262
1.269MetGln: 1.269 ± 0.236
1.198MetArg: 1.198 ± 0.249
2.114MetSer: 2.114 ± 0.37
1.762MetThr: 1.762 ± 0.363
1.41MetVal: 1.41 ± 0.335
0.282MetTrp: 0.282 ± 0.129
1.057MetTyr: 1.057 ± 0.246
0.0MetXaa: 0.0 ± 0.0
Asn
4.581AsnAla: 4.581 ± 0.738
0.423AsnCys: 0.423 ± 0.212
4.088AsnAsp: 4.088 ± 0.514
3.736AsnGlu: 3.736 ± 0.495
1.41AsnPhe: 1.41 ± 0.389
3.877AsnGly: 3.877 ± 0.572
1.057AsnHis: 1.057 ± 0.277
3.947AsnIle: 3.947 ± 0.605
3.947AsnLys: 3.947 ± 0.663
3.242AsnLeu: 3.242 ± 0.508
0.916AsnMet: 0.916 ± 0.215
3.524AsnAsn: 3.524 ± 0.51
2.96AsnPro: 2.96 ± 0.49
1.973AsnGln: 1.973 ± 0.324
1.833AsnArg: 1.833 ± 0.334
4.299AsnSer: 4.299 ± 0.649
3.947AsnThr: 3.947 ± 0.44
3.806AsnVal: 3.806 ± 0.589
0.916AsnTrp: 0.916 ± 0.252
2.255AsnTyr: 2.255 ± 0.343
0.0AsnXaa: 0.0 ± 0.0
Pro
3.454ProAla: 3.454 ± 0.561
0.775ProCys: 0.775 ± 0.252
2.326ProAsp: 2.326 ± 0.455
2.819ProGlu: 2.819 ± 0.545
1.48ProPhe: 1.48 ± 0.31
0.634ProGly: 0.634 ± 0.188
0.423ProHis: 0.423 ± 0.171
2.185ProIle: 2.185 ± 0.358
2.537ProLys: 2.537 ± 0.502
2.89ProLeu: 2.89 ± 0.433
0.705ProMet: 0.705 ± 0.224
1.551ProAsn: 1.551 ± 0.331
1.128ProPro: 1.128 ± 0.276
1.48ProGln: 1.48 ± 0.341
1.621ProArg: 1.621 ± 0.284
2.608ProSer: 2.608 ± 0.433
1.973ProThr: 1.973 ± 0.334
1.973ProVal: 1.973 ± 0.31
0.282ProTrp: 0.282 ± 0.117
1.551ProTyr: 1.551 ± 0.305
0.0ProXaa: 0.0 ± 0.0
Gln
3.242GlnAla: 3.242 ± 0.454
0.352GlnCys: 0.352 ± 0.14
1.833GlnAsp: 1.833 ± 0.384
2.537GlnGlu: 2.537 ± 0.387
1.269GlnPhe: 1.269 ± 0.269
2.114GlnGly: 2.114 ± 0.391
0.352GlnHis: 0.352 ± 0.151
2.114GlnIle: 2.114 ± 0.491
1.621GlnLys: 1.621 ± 0.29
2.96GlnLeu: 2.96 ± 0.448
1.269GlnMet: 1.269 ± 0.304
2.255GlnAsn: 2.255 ± 0.475
1.692GlnPro: 1.692 ± 0.397
2.114GlnGln: 2.114 ± 0.504
1.41GlnArg: 1.41 ± 0.323
4.088GlnSer: 4.088 ± 0.439
2.114GlnThr: 2.114 ± 0.311
2.467GlnVal: 2.467 ± 0.405
0.705GlnTrp: 0.705 ± 0.211
1.48GlnTyr: 1.48 ± 0.321
0.0GlnXaa: 0.0 ± 0.0
Arg
2.96ArgAla: 2.96 ± 0.42
0.564ArgCys: 0.564 ± 0.169
2.185ArgAsp: 2.185 ± 0.437
2.819ArgGlu: 2.819 ± 0.599
1.903ArgPhe: 1.903 ± 0.441
2.044ArgGly: 2.044 ± 0.426
0.634ArgHis: 0.634 ± 0.266
2.96ArgIle: 2.96 ± 0.359
3.031ArgLys: 3.031 ± 0.44
3.242ArgLeu: 3.242 ± 0.547
1.339ArgMet: 1.339 ± 0.257
1.339ArgAsn: 1.339 ± 0.271
1.128ArgPro: 1.128 ± 0.306
1.762ArgGln: 1.762 ± 0.461
1.551ArgArg: 1.551 ± 0.316
2.467ArgSer: 2.467 ± 0.392
2.608ArgThr: 2.608 ± 0.47
2.96ArgVal: 2.96 ± 0.402
0.352ArgTrp: 0.352 ± 0.16
2.044ArgTyr: 2.044 ± 0.416
0.0ArgXaa: 0.0 ± 0.0
Ser
5.216SerAla: 5.216 ± 0.715
1.198SerCys: 1.198 ± 0.375
4.44SerAsp: 4.44 ± 0.464
3.454SerGlu: 3.454 ± 0.475
3.454SerPhe: 3.454 ± 0.372
5.991SerGly: 5.991 ± 0.945
1.057SerHis: 1.057 ± 0.312
4.652SerIle: 4.652 ± 0.649
5.427SerLys: 5.427 ± 0.753
4.863SerLeu: 4.863 ± 0.637
1.339SerMet: 1.339 ± 0.261
3.806SerAsn: 3.806 ± 0.715
2.185SerPro: 2.185 ± 0.337
2.396SerGln: 2.396 ± 0.417
2.819SerArg: 2.819 ± 0.542
4.652SerSer: 4.652 ± 0.615
4.158SerThr: 4.158 ± 0.612
5.568SerVal: 5.568 ± 0.685
0.775SerTrp: 0.775 ± 0.234
2.89SerTyr: 2.89 ± 0.461
0.0SerXaa: 0.0 ± 0.0
Thr
3.947ThrAla: 3.947 ± 0.655
0.564ThrCys: 0.564 ± 0.206
3.595ThrAsp: 3.595 ± 0.463
4.44ThrGlu: 4.44 ± 0.443
2.749ThrPhe: 2.749 ± 0.397
5.357ThrGly: 5.357 ± 0.653
0.987ThrHis: 0.987 ± 0.285
3.947ThrIle: 3.947 ± 0.514
3.595ThrLys: 3.595 ± 0.529
4.722ThrLeu: 4.722 ± 0.533
1.269ThrMet: 1.269 ± 0.273
3.524ThrAsn: 3.524 ± 0.536
2.608ThrPro: 2.608 ± 0.398
2.185ThrGln: 2.185 ± 0.37
2.044ThrArg: 2.044 ± 0.356
4.017ThrSer: 4.017 ± 0.58
2.96ThrThr: 2.96 ± 0.468
4.229ThrVal: 4.229 ± 0.595
0.775ThrTrp: 0.775 ± 0.303
2.255ThrTyr: 2.255 ± 0.484
0.0ThrXaa: 0.0 ± 0.0
Val
4.793ValAla: 4.793 ± 0.589
0.705ValCys: 0.705 ± 0.214
6.343ValAsp: 6.343 ± 0.639
4.511ValGlu: 4.511 ± 0.594
2.185ValPhe: 2.185 ± 0.318
3.736ValGly: 3.736 ± 0.539
0.423ValHis: 0.423 ± 0.127
3.665ValIle: 3.665 ± 0.539
4.44ValLys: 4.44 ± 0.451
4.229ValLeu: 4.229 ± 0.524
1.48ValMet: 1.48 ± 0.379
4.581ValAsn: 4.581 ± 0.478
1.833ValPro: 1.833 ± 0.327
2.114ValGln: 2.114 ± 0.388
2.185ValArg: 2.185 ± 0.467
4.652ValSer: 4.652 ± 0.772
4.299ValThr: 4.299 ± 0.719
3.947ValVal: 3.947 ± 0.527
1.057ValTrp: 1.057 ± 0.255
2.608ValTyr: 2.608 ± 0.433
0.0ValXaa: 0.0 ± 0.0
Trp
0.634TrpAla: 0.634 ± 0.221
0.493TrpCys: 0.493 ± 0.19
0.705TrpAsp: 0.705 ± 0.207
0.564TrpGlu: 0.564 ± 0.216
0.987TrpPhe: 0.987 ± 0.276
0.423TrpGly: 0.423 ± 0.154
0.634TrpHis: 0.634 ± 0.238
1.057TrpIle: 1.057 ± 0.284
0.634TrpLys: 0.634 ± 0.28
1.198TrpLeu: 1.198 ± 0.28
0.352TrpMet: 0.352 ± 0.161
0.846TrpAsn: 0.846 ± 0.265
0.564TrpPro: 0.564 ± 0.181
0.634TrpGln: 0.634 ± 0.171
0.705TrpArg: 0.705 ± 0.187
0.634TrpSer: 0.634 ± 0.215
0.493TrpThr: 0.493 ± 0.195
1.057TrpVal: 1.057 ± 0.279
0.211TrpTrp: 0.211 ± 0.122
0.564TrpTyr: 0.564 ± 0.199
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.608TyrAla: 2.608 ± 0.341
0.352TyrCys: 0.352 ± 0.139
3.313TyrAsp: 3.313 ± 0.552
2.467TyrGlu: 2.467 ± 0.423
1.551TyrPhe: 1.551 ± 0.3
3.242TyrGly: 3.242 ± 0.523
0.846TyrHis: 0.846 ± 0.333
2.608TyrIle: 2.608 ± 0.475
2.678TyrLys: 2.678 ± 0.464
2.255TyrLeu: 2.255 ± 0.438
0.564TyrMet: 0.564 ± 0.202
1.973TyrAsn: 1.973 ± 0.319
1.48TyrPro: 1.48 ± 0.309
2.255TyrGln: 2.255 ± 0.443
1.973TyrArg: 1.973 ± 0.317
2.89TyrSer: 2.89 ± 0.367
2.467TyrThr: 2.467 ± 0.44
1.903TyrVal: 1.903 ± 0.324
0.493TyrTrp: 0.493 ± 0.207
1.833TyrTyr: 1.833 ± 0.472
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 73 proteins (14189 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski