Rhizopus microsporus

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Mucoromycota; Mucoromycotina; Mucoromycetes; Mucorales; Mucorineae; Rhizopodaceae; Rhizopus

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11322 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A1NS01|A0A0A1NS01_RHIZD Precorrin-2 dehydrogenase OS=Rhizopus microsporus OX=58291 GN=BCV71DRAFT_32954 PE=3 SV=1
MM1 pKa = 7.43FNNDD5 pKa = 2.77QTMSNGFLQEE15 pKa = 3.71PVAFEE20 pKa = 4.64DD21 pKa = 4.91FEE23 pKa = 4.45FTLGYY28 pKa = 10.89DD29 pKa = 4.09FAAPIDD35 pKa = 4.03FNEE38 pKa = 5.09LEE40 pKa = 5.03DD41 pKa = 3.93IPFNSEE47 pKa = 3.47AHH49 pKa = 5.44SHH51 pKa = 6.14VLDD54 pKa = 3.78EE55 pKa = 5.56DD56 pKa = 3.57EE57 pKa = 6.09RR58 pKa = 11.84DD59 pKa = 3.51MFNQFLDD66 pKa = 3.58QFDD69 pKa = 4.01TDD71 pKa = 4.51GDD73 pKa = 3.92VHH75 pKa = 6.67MDD77 pKa = 3.8PSAFSFPVQVDD88 pKa = 3.33EE89 pKa = 6.64DD90 pKa = 4.46GLCEE94 pKa = 4.32SFLLHH99 pKa = 7.35SLDD102 pKa = 4.06DD103 pKa = 4.1RR104 pKa = 11.84NNVIEE109 pKa = 5.07DD110 pKa = 3.34NSEE113 pKa = 4.2YY114 pKa = 10.98EE115 pKa = 4.27HH116 pKa = 6.67GASLLKK122 pKa = 10.51EE123 pKa = 3.82ATNRR127 pKa = 11.84LGYY130 pKa = 10.19LCSSTQSSGSISSDD144 pKa = 2.84LNTSNTNHH152 pKa = 6.19YY153 pKa = 10.93GEE155 pKa = 4.91LTVPMM160 pKa = 5.3

Molecular weight: 18.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A1NT65|A0A0A1NT65_RHIZD Uncharacterized protein OS=Rhizopus microsporus OX=58291 GN=BCV71DRAFT_228752 PE=4 SV=1
MM1 pKa = 7.57FGQLLTTATLRR12 pKa = 11.84FSSLTNAVRR21 pKa = 11.84PTLMGQSMFSNPLSNVLTTQMRR43 pKa = 11.84FVTRR47 pKa = 11.84GNTYY51 pKa = 10.12QPSQLVRR58 pKa = 11.84KK59 pKa = 9.04RR60 pKa = 11.84RR61 pKa = 11.84HH62 pKa = 5.16GFLTRR67 pKa = 11.84LATKK71 pKa = 10.26NGRR74 pKa = 11.84HH75 pKa = 5.88IINRR79 pKa = 11.84RR80 pKa = 11.84RR81 pKa = 11.84MKK83 pKa = 10.08GRR85 pKa = 11.84KK86 pKa = 8.57FLSHH90 pKa = 6.98

Molecular weight: 10.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11322

0

11322

4154363

49

5889

366.9

41.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.379 ± 0.019

1.475 ± 0.011

5.64 ± 0.018

6.599 ± 0.029

4.053 ± 0.016

4.848 ± 0.022

2.56 ± 0.009

6.408 ± 0.017

6.679 ± 0.022

9.268 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.431 ± 0.009

4.852 ± 0.016

4.69 ± 0.02

4.609 ± 0.021

5.056 ± 0.015

7.86 ± 0.027

5.986 ± 0.018

5.896 ± 0.022

1.163 ± 0.008

3.549 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski