Streptococcus phage Javan618

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 38 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6BG54|A0A4D6BG54_9CAUD Uncharacterized protein OS=Streptococcus phage Javan618 OX=2548284 GN=Javan618_0037 PE=4 SV=1
MM1 pKa = 7.63PVGLIPKK8 pKa = 8.64EE9 pKa = 3.94FLVTSVGCDD18 pKa = 3.29MVNHH22 pKa = 6.01YY23 pKa = 10.68CLGQSTFDD31 pKa = 3.38FTLNTEE37 pKa = 3.95WMNFF41 pKa = 3.37

Molecular weight: 4.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6BEY4|A0A4D6BEY4_9CAUD Uncharacterized protein OS=Streptococcus phage Javan618 OX=2548284 GN=Javan618_0036 PE=4 SV=1
MM1 pKa = 8.11DD2 pKa = 4.52EE3 pKa = 4.27SQRR6 pKa = 11.84KK7 pKa = 8.66QIWKK11 pKa = 8.66MRR13 pKa = 11.84AEE15 pKa = 4.01GLGYY19 pKa = 10.92GLIGKK24 pKa = 7.93ATGLSRR30 pKa = 11.84DD31 pKa = 3.81SVKK34 pKa = 10.54KK35 pKa = 9.25FCKK38 pKa = 10.07RR39 pKa = 11.84NPALLGHH46 pKa = 6.6GAATKK51 pKa = 10.58QMAKK55 pKa = 10.35ADD57 pKa = 3.83QNDD60 pKa = 3.76GLRR63 pKa = 11.84CQQCYY68 pKa = 7.92QALKK72 pKa = 6.82THH74 pKa = 7.18KK75 pKa = 9.72IGRR78 pKa = 11.84PKK80 pKa = 10.36KK81 pKa = 9.58FCSDD85 pKa = 3.07KK86 pKa = 10.71CRR88 pKa = 11.84KK89 pKa = 9.06VWWTTHH95 pKa = 5.85SDD97 pKa = 3.15EE98 pKa = 4.68HH99 pKa = 6.99DD100 pKa = 3.59KK101 pKa = 11.69SKK103 pKa = 9.37TAYY106 pKa = 9.78EE107 pKa = 4.67DD108 pKa = 3.74LTCQQCGRR116 pKa = 11.84SFLSYY121 pKa = 11.06ANPNRR126 pKa = 11.84KK127 pKa = 8.69FCSHH131 pKa = 5.91SCYY134 pKa = 9.75IQSRR138 pKa = 11.84FYY140 pKa = 10.91KK141 pKa = 10.77GEE143 pKa = 4.11NNDD146 pKa = 3.05KK147 pKa = 9.83STNNGNN153 pKa = 3.32

Molecular weight: 17.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

38

0

38

11931

39

1039

314.0

35.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.739 ± 0.454

0.897 ± 0.15

6.068 ± 0.29

7.149 ± 0.322

3.897 ± 0.24

6.906 ± 0.327

2.037 ± 0.146

6.295 ± 0.317

7.242 ± 0.293

9.178 ± 0.264

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.33 ± 0.157

4.719 ± 0.247

2.95 ± 0.267

4.132 ± 0.206

4.392 ± 0.306

7.04 ± 0.426

6.362 ± 0.544

6.471 ± 0.233

1.433 ± 0.105

3.763 ± 0.301
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski